##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939600_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2255327 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.493187018999905 31.0 31.0 34.0 30.0 34.0 2 31.68892803571278 31.0 31.0 34.0 30.0 34.0 3 31.737144103715337 31.0 31.0 34.0 30.0 34.0 4 35.539359037514295 37.0 35.0 37.0 33.0 37.0 5 35.30306691668215 37.0 35.0 37.0 33.0 37.0 6 35.31242387467538 37.0 35.0 37.0 32.0 37.0 7 35.21801583539771 37.0 35.0 37.0 32.0 37.0 8 35.182733590295335 37.0 35.0 37.0 32.0 37.0 9 36.773678495402216 39.0 35.0 39.0 32.0 39.0 10-11 36.65539187887167 39.0 35.0 39.0 32.0 39.0 12-13 36.599547870441846 39.0 35.0 39.0 32.0 39.0 14-15 37.639011549101305 39.0 36.0 41.0 32.0 41.0 16-17 37.604698786473094 39.0 36.0 41.0 32.0 41.0 18-19 37.61534979184837 39.0 36.5 41.0 32.0 41.0 20-21 37.5674101804306 39.0 36.0 41.0 32.0 41.0 22-23 37.42371749196458 39.0 36.0 41.0 32.0 41.0 24-25 37.28618377734138 39.0 36.0 41.0 31.0 41.0 26-27 36.99747375879418 39.0 36.0 40.0 31.0 41.0 28-29 36.798922284883744 39.0 36.0 40.0 30.5 41.0 30-31 36.52776204958305 38.0 35.0 40.0 30.0 41.0 32-33 36.31957228375309 38.0 35.0 40.0 30.0 41.0 34-35 36.250979126308515 38.0 35.0 40.0 30.0 41.0 36-37 36.133918274378836 38.0 35.0 40.0 30.0 41.0 38-39 36.15042120277902 38.0 35.0 40.0 30.0 41.0 40-41 36.037446676246944 38.0 35.0 40.0 29.5 41.0 42-43 35.822295613895456 38.0 35.0 40.0 29.0 41.0 44-45 35.56402619221071 38.0 35.0 40.0 27.5 41.0 46-47 35.2019749242571 38.0 34.0 40.0 26.5 41.0 48-49 34.843359078306605 38.0 34.0 40.0 25.5 41.0 50-51 34.578530962472406 38.0 33.0 40.0 24.0 41.0 52-53 34.35442820486786 38.0 33.0 40.0 24.0 41.0 54-55 34.08201781825873 38.0 33.0 40.0 23.5 41.0 56-57 33.36869731085559 37.0 32.5 40.0 20.5 41.0 58-59 30.327611472748742 35.5 26.5 39.0 7.5 40.5 60-61 30.076344361593684 34.5 26.0 39.0 10.0 40.0 62-63 31.794085513985333 35.5 30.5 39.0 13.5 40.0 64-65 31.830902126387883 35.5 31.0 39.0 10.0 40.0 66-67 27.059973121414323 31.5 18.5 37.0 2.0 39.5 68-69 19.48860941229365 19.0 8.0 32.0 2.0 37.0 70-71 17.566676140533055 15.5 7.5 29.0 2.0 35.0 72-73 19.29929628829877 19.5 7.5 30.5 2.0 35.5 74-75 20.785663231983655 23.5 9.0 32.5 2.0 36.0 76-77 22.646815073823 27.5 11.5 33.0 2.0 35.5 78-79 24.90507895307421 30.5 13.0 34.5 2.0 36.5 80-81 26.480482652848124 32.0 22.5 35.0 2.0 36.5 82-83 27.50473767218678 33.0 26.0 35.0 2.0 36.0 84-85 27.516647475066808 34.0 26.5 35.0 2.0 36.0 86-87 27.259381233852118 33.0 26.0 35.0 2.0 36.0 88-89 26.834890683257903 33.0 25.5 35.0 2.0 35.0 90-91 25.1167283059175 32.5 16.0 34.5 2.0 35.0 92-93 23.627402146118946 31.5 2.0 34.0 2.0 35.0 94-95 19.79484172361702 27.5 2.0 34.0 2.0 35.0 96-97 17.093073199584804 17.5 2.0 33.0 2.0 35.0 98-99 17.3956579245493 21.5 2.0 33.0 2.0 35.0 100-101 16.626146674074313 10.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.321275091453721 1101 2 0.24155419394913835 1101 3 0.24945618669874747 1101 4 0.2918684577278299 1101 5 0.4195198768715258 1101 6 0.31583491181706336 1101 7 0.3707498696423528 1101 8 0.4372710289084125 1101 9 0.4320038556128267 1101 10-11 0.44823444356337916 1101 12-13 0.25838332607968795 1101 14-15 0.38499033720891873 1101 16-17 0.41920926541963865 1101 18-19 0.46283495508482275 1101 20-21 0.5114726301650379 1101 22-23 0.38607182047119437 1101 24-25 0.3975432559884311 1101 26-27 0.5071844118565494 1101 28-29 0.5401477259270351 1101 30-31 0.33540151882809965 1101 32-33 0.39729960783559193 1101 34-35 0.24741349302602345 1101 36-37 0.1271887126522131 1101 38-39 0.08286043288264011 1101 40-41 0.16317110794610556 1101 42-43 0.20153588816168622 1101 44-45 0.10467630699613295 1101 46-47 -0.02060875523876149 1101 48-49 -0.09238824843539817 1101 50-51 0.004160135458768366 1101 52-53 -0.02269861352213809 1101 54-55 0.02773069267166761 1101 56-57 -3.199978316119651 1101 58-59 -9.909212606473165 1101 60-61 -11.554941704866742 1101 62-63 -3.8911707080068254 1101 64-65 -0.9213677316092017 1101 66-67 -2.0621555072914717 1101 68-69 -4.759090802305236 1101 70-71 -4.664302275681521 1101 72-73 -3.511308177995943 1101 74-75 -6.044808840124023 1101 76-77 -4.756900509511862 1101 78-79 1.2443484359649268 1101 80-81 2.015178931070622 1101 82-83 0.5531910761405427 1101 84-85 0.319831523437589 1101 86-87 0.3171702441974844 1101 88-89 0.19981512524372746 1101 90-91 1.6537530292362987 1101 92-93 0.8636097632925832 1101 94-95 -8.589607261055455 1101 96-97 -9.53624542084961 1101 98-99 -8.819711513748736 1101 100-101 -9.748916948510125 1102 1 0.29450787130095435 1102 2 0.3244167171986305 1102 3 0.4000051891397014 1102 4 0.306433529030393 1102 5 0.4011418658335302 1102 6 0.4210354890426089 1102 7 0.37323347234812587 1102 8 0.38374539625261406 1102 9 0.31856503547940207 1102 10-11 0.4146220571028678 1102 12-13 0.3393312671524953 1102 14-15 0.4050899347302277 1102 16-17 0.43203521219949437 1102 18-19 0.37951791075283126 1102 20-21 0.3366291938651287 1102 22-23 0.4063276378852052 1102 24-25 0.44445735683478205 1102 26-27 0.07905491097370287 1102 28-29 0.33692627395353014 1102 30-31 0.4384461533701227 1102 32-33 0.16172255921102874 1102 34-35 0.1566837297988286 1102 36-37 0.03796088548548937 1102 38-39 0.22617650064714923 1102 40-41 0.13236627572951676 1102 42-43 0.1745782991584548 1102 44-45 0.1668862822929924 1102 46-47 0.229653967681692 1102 48-49 0.28586878567044494 1102 50-51 0.28119401879634864 1102 52-53 0.2240574286414727 1102 54-55 0.1820861450008806 1102 56-57 -3.1394556238281837 1102 58-59 -9.558202654910605 1102 60-61 -7.612809122542643 1102 62-63 -2.0569195506121147 1102 64-65 -0.5870905375657074 1102 66-67 -1.7227182666632395 1102 68-69 -4.703787028783598 1102 70-71 -4.5360365498160675 1102 72-73 -3.410751627244643 1102 74-75 -3.9400504223673103 1102 76-77 -3.7826272680943376 1102 78-79 2.0120011679510803 1102 80-81 2.246075795500957 1102 82-83 0.8027219915319392 1102 84-85 0.4506921837919009 1102 86-87 0.4930827282992887 1102 88-89 0.48445062838535335 1102 90-91 1.7034162757020006 1102 92-93 1.2882444042116568 1102 94-95 -8.666006282819158 1102 96-97 -9.66631941901422 1102 98-99 -8.706754873808393 1102 100-101 -9.46860051592349 1103 1 0.1360396701249975 1103 2 0.11344752221121013 1103 3 0.21126962240195013 1103 4 0.17295142675849462 1103 5 0.17619645455962285 1103 6 0.2248671477288937 1103 7 0.27607343520494254 1103 8 0.24960407465277257 1103 9 0.25061010093911307 1103 10-11 0.19511512863108038 1103 12-13 0.24552106068959745 1103 14-15 0.3484017483851787 1103 16-17 0.344310826588071 1103 18-19 0.2489443454785416 1103 20-21 0.2942680664651647 1103 22-23 0.2858212715895334 1103 24-25 0.34127105853987416 1103 26-27 0.26440983236026483 1103 28-29 0.26801669784229176 1103 30-31 0.3061507991953434 1103 32-33 0.20994820906714295 1103 34-35 0.2402288181779113 1103 36-37 0.26319359374092244 1103 38-39 0.27729505020518985 1103 40-41 0.21710048846143337 1103 42-43 0.26329519312547234 1103 44-45 0.3108288155379526 1103 46-47 0.291459152426377 1103 48-49 0.17087793885416147 1103 50-51 0.02659339609470379 1103 52-53 0.17707325741737634 1103 54-55 -0.04009897697079623 1103 56-57 0.3462810928640323 1103 58-59 -8.415405246961004 1103 60-61 -7.4095222856348855 1103 62-63 -2.177478194533567 1103 64-65 -0.37085065409115714 1103 66-67 -1.7578646984393238 1103 68-69 -3.398855470313986 1103 70-71 -3.7913863829492573 1103 72-73 -2.7979371240514936 1103 74-75 -3.495168823524345 1103 76-77 -0.4088754347363981 1103 78-79 3.26027458821245 1103 80-81 2.6168041117459495 1103 82-83 1.149417010761983 1103 84-85 0.7583137403865727 1103 86-87 0.5287714062993167 1103 88-89 0.3889768896137369 1103 90-91 1.6447545706572697 1103 92-93 1.318202089996145 1103 94-95 -7.262638484579519 1103 96-97 -8.817953892015126 1103 98-99 -7.984996634678268 1103 100-101 -9.308158412780806 1104 1 0.17816898900848344 1104 2 0.2524442733987762 1104 3 0.25955263951383856 1104 4 0.1389148620556 1104 5 0.27254892143203335 1104 6 0.2635077376062327 1104 7 0.3068770586385554 1104 8 0.2831748283143867 1104 9 0.33221563433667 1104 10-11 0.3036780699804922 1104 12-13 0.29482437810246154 1104 14-15 0.45632052067799833 1104 16-17 0.39356968932497693 1104 18-19 0.4004749269056376 1104 20-21 0.42558918239001997 1104 22-23 0.41523977361026 1104 24-25 0.38300575549920524 1104 26-27 0.4548338637105971 1104 28-29 0.4455642820657957 1104 30-31 0.46200997171276725 1104 32-33 0.49661545819022024 1104 34-35 0.49786901297105857 1104 36-37 0.45219838837419246 1104 38-39 0.46883422363482197 1104 40-41 0.38869153132960577 1104 42-43 0.30622230779665927 1104 44-45 0.2988968569250332 1104 46-47 0.2398421116624334 1104 48-49 0.2870332303628089 1104 50-51 0.33305314589115653 1104 52-53 0.48394773775223143 1104 54-55 0.333303582385831 1104 56-57 0.753323822975922 1104 58-59 -8.017704085314865 1104 60-61 -7.038015714355474 1104 62-63 -1.7204039994482656 1104 64-65 -0.11215448739397971 1104 66-67 -1.6123301051510701 1104 68-69 -3.482728808541186 1104 70-71 -3.636139945332804 1104 72-73 -2.9249251803050704 1104 74-75 -3.6893222708958646 1104 76-77 -0.017382904046492342 1104 78-79 3.8219944741372807 1104 80-81 3.1133858784564374 1104 82-83 1.5922184812641085 1104 84-85 1.2469233275574894 1104 86-87 1.173173214317984 1104 88-89 1.0440006686279268 1104 90-91 2.5903346510177627 1104 92-93 2.3427950022720445 1104 94-95 -5.796797627080405 1104 96-97 -7.417588454547214 1104 98-99 -5.279721428645868 1104 100-101 -4.7141180513249985 1105 1 0.16152820441780946 1105 2 0.24081317577087447 1105 3 0.2871363365656059 1105 4 0.24802427612763012 1105 5 0.25759167389673365 1105 6 0.23362711084186571 1105 7 0.22914741427458551 1105 8 0.20738100852764063 1105 9 0.21187276163948354 1105 10-11 0.2092148167791521 1105 12-13 0.26789680108498715 1105 14-15 0.33961653092880795 1105 16-17 0.41542164594093833 1105 18-19 0.3890176395437237 1105 20-21 0.3513304122234828 1105 22-23 0.44927828375600853 1105 24-25 0.42199005753926855 1105 26-27 0.3807426319014766 1105 28-29 0.33984459937419587 1105 30-31 0.4245353180632421 1105 32-33 0.3023019311259958 1105 34-35 0.42138841919693704 1105 36-37 0.43550460615617936 1105 38-39 0.5112783456334782 1105 40-41 0.4604424788581909 1105 42-43 0.3611199314332225 1105 44-45 0.27495815793211165 1105 46-47 0.3433527497601432 1105 48-49 0.3077528722870113 1105 50-51 0.2967430288636095 1105 52-53 0.34814327767261233 1105 54-55 0.1974528669805835 1105 56-57 0.7878014451197686 1105 58-59 -7.936808616706077 1105 60-61 -7.105824610556013 1105 62-63 -1.941761917826902 1105 64-65 -0.11453608787214975 1105 66-67 -1.2399559180688193 1105 68-69 -3.3877856759361045 1105 70-71 -3.704685869188072 1105 72-73 -2.862106136156477 1105 74-75 -3.794836705379822 1105 76-77 -0.4320518633047996 1105 78-79 3.587865538749856 1105 80-81 3.1085040600294462 1105 82-83 1.552989339593612 1105 84-85 1.0192014603601365 1105 86-87 0.8429242226840827 1105 88-89 0.8681914669854365 1105 90-91 2.166578793839154 1105 92-93 1.9270072913242373 1105 94-95 -6.250193504931225 1105 96-97 -7.715384944912623 1105 98-99 -5.536859958171817 1105 100-101 -4.85791990040908 1106 1 0.15252759276813777 1106 2 0.1735677674684446 1106 3 0.0878743582645356 1106 4 0.1503341388532604 1106 5 0.1112581684779812 1106 6 0.20719294758039553 1106 7 0.2598613467098545 1106 8 0.182866193202031 1106 9 0.13375525214500783 1106 10-11 0.17670296091311144 1106 12-13 0.269200214730418 1106 14-15 0.17225669902818197 1106 16-17 0.3216963987143373 1106 18-19 0.30252830654905694 1106 20-21 0.31953255184937035 1106 22-23 0.37034815167492496 1106 24-25 0.28467834434298567 1106 26-27 0.35901411284228146 1106 28-29 0.4870638084398635 1106 30-31 0.3887825111927157 1106 32-33 0.5132507350946156 1106 34-35 0.3268300155459869 1106 36-37 0.42146940469093863 1106 38-39 0.43060227145102914 1106 40-41 0.3503411726635832 1106 42-43 0.4623037314317031 1106 44-45 0.48974420261858853 1106 46-47 0.35703451052217616 1106 48-49 0.2638605814819499 1106 50-51 0.3413171400920163 1106 52-53 0.43804882686950464 1106 54-55 0.5081977392204919 1106 56-57 1.1238971994173639 1106 58-59 -8.342574227313701 1106 60-61 -7.600539895960999 1106 62-63 -1.986463920910861 1106 64-65 -0.10111989268880706 1106 66-67 0.5401996799161459 1106 68-69 -2.1945235395083174 1106 70-71 -2.4806466628160493 1106 72-73 -1.0887169227592963 1106 74-75 -1.0439641385385414 1106 76-77 2.2834573928480353 1106 78-79 4.663377595265448 1106 80-81 3.746077296710382 1106 82-83 2.1732457603387374 1106 84-85 1.6202039320198018 1106 86-87 1.505452377890144 1106 88-89 1.6562594640615451 1106 90-91 3.113313291155446 1106 92-93 2.584544349646645 1106 94-95 -6.008551221523195 1106 96-97 -7.625305666513718 1106 98-99 -5.617385585613951 1106 100-101 -5.07757480905533 1107 1 0.016917794143843423 1107 2 -0.02211503057229436 1107 3 0.02455302636296608 1107 4 0.05350793421169442 1107 5 0.16201177518059495 1107 6 0.06821257172283168 1107 7 0.14327669125667342 1107 8 0.1909050721790848 1107 9 0.1715727909207203 1107 10-11 0.006454710782342943 1107 12-13 0.07009560925604319 1107 14-15 0.036482835084008514 1107 16-17 0.032164940315190904 1107 18-19 0.0950899870669204 1107 20-21 0.10059067987050696 1107 22-23 0.09185917396914789 1107 24-25 0.1327892447784933 1107 26-27 -0.06735224566039477 1107 28-29 0.05731376356317952 1107 30-31 0.07085496501154154 1107 32-33 -0.0383716135615515 1107 34-35 -0.13198251183845144 1107 36-37 -0.044481572591479335 1107 38-39 -0.07000074902367004 1107 40-41 -0.03116393684518215 1107 42-43 -0.12451360403169076 1107 44-45 -0.11286691303201479 1107 46-47 -0.10276707338372404 1107 48-49 -0.23924692827029048 1107 50-51 -0.10601499695849981 1107 52-53 -0.15218010215712496 1107 54-55 -0.23339045489644406 1107 56-57 0.2944165594468089 1107 58-59 -8.24315538849726 1107 60-61 -7.592575168074589 1107 62-63 -2.394241517823602 1107 64-65 -0.667509165647818 1107 66-67 0.5322837695048186 1107 68-69 -2.5479066468282667 1107 70-71 -2.5279148465614867 1107 72-73 -1.013544203798041 1107 74-75 -0.7651241093027465 1107 76-77 2.170730283016276 1107 78-79 4.038640904019008 1107 80-81 3.00710594444541 1107 82-83 1.4161091601968039 1107 84-85 1.0409870029236856 1107 86-87 0.9792841202188072 1107 88-89 0.9456071532176047 1107 90-91 2.3518374849995887 1107 92-93 1.5691778631086883 1107 94-95 -6.772907821677997 1107 96-97 -8.17668408864285 1107 98-99 -6.144487085428331 1107 100-101 -5.3576655950826275 1108 1 -0.05514760373180749 1108 2 -0.0350081591349749 1108 3 -0.10116723504691194 1108 4 -0.07224716965667 1108 5 -0.07416380936864897 1108 6 -0.09836244298667651 1108 7 -0.05184344112574735 1108 8 -0.10184189463512894 1108 9 -0.015238124575127188 1108 10-11 -0.09547500961402733 1108 12-13 -0.13041950732588248 1108 14-15 -0.30764855976838845 1108 16-17 -0.17802203283057594 1108 18-19 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2312 100-101 -0.261580185550093 2313 1 -0.28500214448240513 2313 2 -0.396316212004308 2313 3 -0.29709655908997235 2313 4 -0.2854685449219332 2313 5 -0.2703854234620877 2313 6 -0.2170245660307657 2313 7 -0.3357725958728821 2313 8 -0.4231063697151356 2313 9 -0.5160439509007375 2313 10-11 -0.4133454514525212 2313 12-13 -0.44450784002037835 2313 14-15 -0.5068969749660042 2313 16-17 -0.5462328724476677 2313 18-19 -0.5494494610667076 2313 20-21 -0.44188716110489423 2313 22-23 -0.42394628959592495 2313 24-25 -0.4749585539865038 2313 26-27 -0.7341153668193527 2313 28-29 -0.6008670600720976 2313 30-31 -0.6527241566882012 2313 32-33 -0.7096747918725868 2313 34-35 -0.8013795580670902 2313 36-37 -0.8621765238604411 2313 38-39 -0.9632817450334912 2313 40-41 -0.905264040346438 2313 42-43 -0.8060700543023032 2313 44-45 -0.9867074662630984 2313 46-47 -1.1120338789448567 2313 48-49 -1.2529242549917825 2313 50-51 -1.0724478770673187 2313 52-53 -0.9645125822269165 2313 54-55 -1.0349665850720697 2313 56-57 -0.23828502087634718 2313 58-59 2.897070391540577 2313 60-61 2.6043837891837924 2313 62-63 -0.16333966672637956 2313 64-65 -0.8606312269743803 2313 66-67 -2.738568974298655 2313 68-69 -5.095887017302797 2313 70-71 -3.630402766632759 2313 72-73 -2.935907185807448 2313 74-75 -3.856395806780643 2313 76-77 0.17947583574675008 2313 78-79 2.3825519471496293 2313 80-81 1.6000859227248156 2313 82-83 0.1315911265547527 2313 84-85 -0.19406103619634152 2313 86-87 -0.20730302968327408 2313 88-89 -0.05668368083156494 2313 90-91 1.6145682822653207 2313 92-93 2.937821830924456 2313 94-95 5.213304048506053 2313 96-97 1.1605534196235396 2313 98-99 2.575883369970793 2313 100-101 3.731471986967465 2314 1 -0.3560068351673422 2314 2 -0.25997330901880744 2314 3 -0.31992930711096434 2314 4 -0.28527666070735336 2314 5 -0.3691465259777047 2314 6 -0.32707311011747464 2314 7 -0.2473144641244005 2314 8 -0.3876257996290988 2314 9 -0.41685814902282203 2314 10-11 -0.47589806816707636 2314 12-13 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2314 78-79 3.2405031083602047 2314 80-81 2.3236763023182903 2314 82-83 0.7885842583441729 2314 84-85 0.626766341686217 2314 86-87 0.61825170725362 2314 88-89 0.594463776948178 2314 90-91 2.342187084298768 2314 92-93 4.158493589399203 2314 94-95 6.664560300706093 2314 96-97 3.820119551732219 2314 98-99 5.958269152201858 2314 100-101 6.388927474577672 2315 1 -0.4672813181148818 2315 2 -0.4183002505336688 2315 3 -0.5207336489819383 2315 4 -0.3701684563517844 2315 5 -0.34162735052228754 2315 6 -0.5378695763549786 2315 7 -0.5571833177060981 2315 8 -0.5766290183662548 2315 9 -0.7627022114800255 2315 10-11 -0.5669633331992614 2315 12-13 -0.5673359028917133 2315 14-15 -0.7764959796960298 2315 16-17 -0.801405577498322 2315 18-19 -0.7104882051205337 2315 20-21 -0.6689496979530318 2315 22-23 -0.7463137675274183 2315 24-25 -0.6423368608968616 2315 26-27 -0.7495039245797344 2315 28-29 -0.846820915430861 2315 30-31 -0.7704242706987046 2315 32-33 -0.7983523676081461 2315 34-35 -0.6988048488813732 2315 36-37 -0.4323715598942215 2315 38-39 -0.717384339681054 2315 40-41 -0.6956001357760542 2315 42-43 -0.7676028663101562 2315 44-45 -0.6400399852356813 2315 46-47 -0.5427288348886137 2315 48-49 -0.8580986930828658 2315 50-51 -0.6093500285949531 2315 52-53 -0.507212258770906 2315 54-55 -0.710209951060591 2315 56-57 -0.02121674312144961 2315 58-59 3.0976663723674385 2315 60-61 3.1382565692094744 2315 62-63 0.29425712442306917 2315 64-65 -0.4080682452564446 2315 66-67 -2.1030867717826176 2315 68-69 -4.949773957783123 2315 70-71 -3.5806361550195707 2315 72-73 -2.9192930487740902 2315 74-75 -0.3965587664233503 2315 76-77 1.709310883948831 2315 78-79 3.367971777035727 2315 80-81 2.2758537437646673 2315 82-83 0.5147366689728514 2315 84-85 0.3750133284003674 2315 86-87 0.1514490432349902 2315 88-89 0.3513946768249063 2315 90-91 1.9299469802034395 2315 92-93 3.5906321663064773 2315 94-95 6.04864998020205 2315 96-97 3.566117017832756 2315 98-99 5.772251825808212 2315 100-101 5.929165798094997 2316 1 -0.421317913631686 2316 2 -0.3589969739921699 2316 3 -0.5129522994822402 2316 4 -0.2078962688281365 2316 5 -0.5310744159541585 2316 6 -0.5340009115936581 2316 7 -0.35988468909209104 2316 8 -0.5382214064754791 2316 9 -0.5418212444084176 2316 10-11 -0.565304762327834 2316 12-13 -0.4446738729959705 2316 14-15 -0.5813314104660634 2316 16-17 -0.7091546214280697 2316 18-19 -0.6181766761066356 2316 20-21 -0.48591190480181723 2316 22-23 -0.8699726134327577 2316 24-25 -0.6808199615912898 2316 26-27 -0.5076214266315802 2316 28-29 -0.44351406926940484 2316 30-31 -0.42113903143376064 2316 32-33 -0.42391316756390296 2316 34-35 -0.5447231056029693 2316 36-37 -0.3488169140040185 2316 38-39 -0.5978468186533306 2316 40-41 -0.7053056312154453 2316 42-43 -0.5427184460350674 2316 44-45 -0.5818277492827875 2316 46-47 -0.5574684330771689 2316 48-49 -0.5922389501886443 2316 50-51 -0.4806163341765384 2316 52-53 -0.5771758276923578 2316 54-55 -0.2821036686458811 2316 56-57 0.5826578516037202 2316 58-59 3.683442571893618 2316 60-61 3.572352297016131 2316 62-63 0.9137460779554374 2316 64-65 0.3476932785187117 2316 66-67 -1.413171130065944 2316 68-69 -2.920567066801322 2316 70-71 -3.177713119401952 2316 72-73 -0.5675374156722306 2316 74-75 5.7021074646517 2316 76-77 5.810970473464362 2316 78-79 4.783168382622552 2316 80-81 3.141317416005741 2316 82-83 1.5324554373448258 2316 84-85 1.1921767174583842 2316 86-87 0.8331818805371576 2316 88-89 0.8752553234019693 2316 90-91 2.350318843507221 2316 92-93 3.716415248956345 2316 94-95 6.359016466520206 2316 96-97 3.443926656534245 2316 98-99 6.064684537089548 2316 100-101 6.210628803851792 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 13.0 4 33.0 5 110.0 6 301.0 7 640.0 8 1348.0 9 2404.0 10 4083.0 11 6393.0 12 8962.0 13 12314.0 14 15154.0 15 19908.0 16 26337.0 17 34645.0 18 35303.0 19 32220.0 20 33224.0 21 33842.0 22 33332.0 23 34160.0 24 36144.0 25 39306.0 26 48992.0 27 68363.0 28 94875.0 29 125236.0 30 153150.0 31 174703.0 32 185188.0 33 186388.0 34 189771.0 35 202044.0 36 202240.0 37 152206.0 38 58305.0 39 3683.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.799625497234025 20.095683593949236 14.440310406200673 22.664380502616062 2 15.301187401552454 22.219921171011368 39.15134669145298 23.3275447359832 3 18.860153388364672 26.625079090736975 31.138582933427216 23.37618458747114 4 12.347300413642898 17.684087495959567 38.26877432851201 31.699837761885526 5 12.39312940182635 39.04810962716081 34.7846881885306 13.774072782482227 6 29.91796754971674 37.9462933756391 17.76150420759384 14.37423486705032 7 26.308114078357598 34.300125879750475 22.051525122521035 17.340234919370896 8 25.550541252129406 34.73001242392452 20.55290454683713 19.166541777108943 9 26.297738642777745 16.28477821619659 21.163981985760824 36.25350115526484 10-11 24.264530154607293 26.86133762421148 28.344714535852226 20.529417685329 12-13 25.788976848579765 24.833638241212135 28.408553796657333 20.968831113550767 14-15 22.48629237436561 25.711209170873765 27.58533844954627 24.217160005214353 16-17 21.031979869863545 29.02570582953676 27.99055568487912 21.951758615720575 18-19 20.99823506237805 27.67342823337082 30.45148128614093 20.8768554181102 20-21 22.173480830052583 26.484319125341916 30.205021267425963 21.137178777179543 22-23 21.60859600403844 26.758071002564147 30.09658909772286 21.53674389567455 24-25 21.72454852989135 26.782197610855903 30.262669285356242 21.230584573896508 26-27 21.59185004559209 26.655974935768693 30.607621826649854 21.14455319198936 28-29 21.19819448324177 27.372312453720333 30.353742944428923 21.075750118608973 30-31 21.479946107026375 26.813350682785487 30.230969570246323 21.475733639941815 32-33 21.288618994725535 27.00240111209402 30.195281581940442 21.51369831124 34-35 21.9167844454421 26.805008230657958 30.122212799920188 21.15599452397976 36-37 21.81441153171065 26.563764137817792 30.225872234291128 21.39595209618043 38-39 21.4456454040386 27.149995151834126 30.16584277431355 21.238516669813723 40-41 21.562426496367536 27.033049452134435 30.076110931127374 21.328413120370655 42-43 21.318019110158687 27.286909126333462 29.384015929348468 22.011055834159382 44-45 21.64898168597013 26.967147122943313 29.39753747665476 21.986333714431797 46-47 21.933899199841296 27.317079557197733 29.084378698146068 21.664642544814903 48-49 21.9121232372772 27.329558716896667 28.94260151215412 21.81571653367201 50-51 21.385106390059537 27.61389188715476 29.131407614646232 21.86959410813947 52-53 21.888891304473212 27.95910435457386 28.343596362366636 21.80840797858629 54-55 21.4454546341801 27.392284958090485 28.65755573517551 22.504704672553903 56-57 21.728353045997004 27.671969621673043 28.493414420298603 22.10626291203135 58-59 21.154004242550965 27.051065194819586 29.90730365692167 21.887626905707787 60-61 21.172471393152158 27.523089272587796 29.738395738296063 21.566043595963983 62-63 21.58908859530297 27.548791600062966 28.836783423603983 22.025336381030083 64-65 21.37599289839868 27.87039311549059 28.679211232732637 22.074402753378095 66-67 21.477912244559768 27.485302747590705 28.675008246427243 22.361776761422284 68-69 20.849288888434394 26.914001915689223 30.119134053932445 22.117575141943934 70-71 21.847967934906677 27.047187887227004 29.003826413268534 22.10101776459778 72-73 21.23108868967424 26.967982585736245 29.515446665202827 22.285482059386688 74-75 20.993246450741506 27.668433779114167 29.09743816248463 22.2408816076597 76-77 19.975588310982758 27.592267606439254 29.510600141537758 22.92154394104023 78-79 21.11267067048542 27.66104370569145 28.596786762648318 22.629498861174813 80-81 21.36046811558106 27.692173595596703 28.232317194748667 22.71504109407357 82-83 21.463897394695746 27.645121184469446 28.253593056778588 22.637388364056218 84-85 21.319670686461542 27.58128362222722 28.091127667192467 23.00791802411877 86-87 21.26183049224159 27.64948709592315 28.11698691302025 22.971695498815013 88-89 21.277190082498052 27.552868188457012 27.80664524773802 23.363296481306918 90-91 21.145394048136502 27.346998064271872 28.198567178575036 23.309040709016596 92-93 21.162075901789603 27.70439277769743 28.210378089261045 22.923153231251924 94-95 20.065896198373753 27.340518549119313 28.9335585850342 23.660026667472735 96-97 19.717570308004852 27.509005663344837 28.815055218119635 23.95836881053068 98-99 20.387172619592235 27.31892735313769 28.35199912878573 23.94190089848435 100-101 19.476255053699848 27.217378059455083 29.08449003376604 24.22187685307903 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1284.0 1 982.0 2 705.0 3 784.5 4 848.5 5 900.0 6 1130.5 7 1662.0 8 2312.5 9 2857.0 10 3144.0 11 3255.5 12 3426.0 13 3613.5 14 3922.5 15 4223.0 16 4439.5 17 4777.5 18 5151.5 19 5633.5 20 6140.0 21 6885.5 22 8044.0 23 9259.5 24 10662.5 25 12830.5 26 15716.5 27 18870.5 28 22375.0 29 26064.5 30 29926.0 31 34365.5 32 39388.0 33 44387.0 34 49338.0 35 54366.0 36 59057.0 37 63014.0 38 65355.0 39 66748.5 40 68598.0 41 70848.5 42 73132.0 43 75518.0 44 78237.0 45 81066.5 46 84967.5 47 90657.0 48 95587.0 49 93877.5 50 86899.5 51 80706.0 52 75792.5 53 71666.5 54 67104.0 55 61579.5 56 55296.5 57 48938.0 58 43218.5 59 36886.0 60 29836.5 61 23477.5 62 18350.5 63 13872.5 64 10007.0 65 7087.0 66 4906.5 67 3487.0 68 2387.0 69 1399.0 70 871.5 71 630.0 72 432.0 73 292.0 74 201.0 75 130.0 76 77.0 77 42.0 78 22.0 79 12.0 80 9.0 81 5.5 82 2.5 83 3.5 84 3.0 85 0.5 86 1.5 87 1.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.026781038847138353 2 0.014144290384498567 3 2.660368097397849E-4 4 0.0 5 0.06353845805951865 6 0.0 7 0.0 8 4.433946828996416E-5 9 0.0 10-11 0.0 12-13 4.433946828996416E-5 14-15 5.764130877695341E-4 16-17 8.867893657992832E-5 18-19 2.8820654388476703E-4 20-21 0.0 22-23 0.0 24-25 2.216973414498208E-5 26-27 6.650920243494623E-5 28-29 7.537709609293907E-4 30-31 0.005165548055780825 32-33 0.005409415131375628 34-35 0.0017514089974535842 36-37 0.0914279836139061 38-39 0.084134141080207 40-41 0.09031949690665698 42-43 0.09433221878689875 44-45 0.09714777502331148 46-47 0.09251430058701021 48-49 0.0821610347413036 50-51 0.07579832104169373 52-53 0.06402619221070825 54-55 0.052542269923607526 56-57 0.07160824128829212 58-59 0.7560322738121789 60-61 0.3530751859929846 62-63 0.0031924417168774197 64-65 0.002704707565687814 66-67 1.1339153923133984 68-69 2.441974046335631 70-71 2.4022902222161133 72-73 1.860883144661506 74-75 1.3193873881703186 76-77 0.7562096316853387 78-79 0.5712918791820432 80-81 0.0410805173706518 82-83 2.2169734144982079E-4 84-85 0.0013080143145539427 86-87 0.0014188629852788532 88-89 4.433946828996416E-5 90-91 0.3367139221939878 92-93 0.058040363991563086 94-95 0.9874177890833568 96-97 1.7082888645415941 98-99 0.6551599834525105 100-101 0.588894648093159 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2255327.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.00598166646342 #Duplication Level Percentage of deduplicated Percentage of total 1 68.42754002407409 30.112210717805965 2 14.948977974787446 13.156889013817239 3 6.3061118258899125 8.32519924190339 4 3.2592296484118806 5.737024006192289 5 1.8844011663807831 4.146246159000752 6 1.1777298182098141 3.1096294072913024 7 0.7898030055408126 2.4329239607362694 8 0.5815297579838735 2.0472630294672998 9 0.4205425839805369 1.6655750316553197 >10 2.010304920144807 15.363673822515134 >50 0.12011202908430665 3.615562495345785 >100 0.06787142616343554 5.489317597014008 >500 0.002922909674182068 0.8628997544824928 >1k 0.002519749719122443 2.1475137657431485 >5k 3.0236996629469316E-4 1.1936890821067654 >10k+ 1.0078998876489772E-4 0.5943829149226307 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13401 0.5941932145538097 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9996 0.44321732502648176 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 9375 0.415682515218414 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7542 0.3344082698429097 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4940 0.21903697335242295 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3995 0.17713617581840682 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3472 0.15394663390275556 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2647 0.11736657256353512 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2610 0.11572601223680645 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2310 0.10242417174981722 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2296 0.10180341919375771 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 4.433946828996416E-5 0.0 0.0 0.0 0.0 6 8.867893657992832E-5 0.0 0.0 0.0 0.0 7 3.1037627802974914E-4 0.0 0.0 0.0 0.0 8 3.1037627802974914E-4 0.0 0.0 0.0 0.0 9 3.1037627802974914E-4 0.0 0.0 4.433946828996416E-5 0.0 10-11 3.1037627802974914E-4 0.0 0.0 4.433946828996416E-5 0.0 12-13 3.3254601217473125E-4 0.0 0.0 4.433946828996416E-5 0.0 14-15 3.547157463197133E-4 0.0 0.0 6.650920243494624E-5 0.0 16-17 3.547157463197133E-4 0.0 0.0 1.5518813901487457E-4 0.0 18-19 3.547157463197133E-4 0.0 0.0 1.7735787315985665E-4 0.0 20-21 3.547157463197133E-4 0.0 0.0 3.1037627802974914E-4 0.0 22-23 3.547157463197133E-4 0.0 0.0 7.316012267844087E-4 0.0 24-25 4.2122494875465957E-4 0.0 0.0 0.0015740511242937276 0.0 26-27 4.655644170446237E-4 0.0 0.0 0.0027933865022677423 0.0 28-29 4.877341511896058E-4 0.0 0.0 0.0064292229020448035 0.0 30-31 4.877341511896058E-4 0.0 0.0 0.01749192024039086 0.0 32-33 5.3207361947957E-4 0.0 0.0 0.039595145182937996 0.0 34-35 6.429222902044803E-4 0.0 0.0 0.069280419203069 0.0 36-37 7.981104292193549E-4 0.0 0.0 0.11102602859807026 0.0 38-39 8.867893657992832E-4 0.0 0.0 0.1817474805205631 0.0 40-41 8.867893657992832E-4 0.0 0.0 0.26330993243995215 0.0 42-43 9.311288340892474E-4 0.0 0.0 0.3421455070595084 0.0 44-45 9.754683023792116E-4 0.0 0.0 0.4337286788124294 0.0 46-47 0.001130656441394086 0.0 0.0 0.5439344272471354 0.0 48-49 0.0011528261755390681 0.0 0.0 0.654406212491581 0.0 50-51 0.0011528261755390681 0.0 0.0 0.7725930652184805 0.0 52-53 0.0011528261755390681 0.0 0.0 0.8858138974969041 0.0 54-55 0.0011528261755390681 4.433946828996416E-5 0.0 1.0115827993013873 0.0 56-57 0.0011971656438290322 4.433946828996416E-5 0.0 1.1351568974255175 0.0 58-59 0.0011971656438290322 4.433946828996416E-5 0.0 1.22474479310539 0.0 60-61 0.0011971656438290322 4.433946828996416E-5 2.216973414498208E-5 1.297816236847251 0.0 62-63 0.0011971656438290322 8.867893657992832E-5 4.433946828996416E-5 1.3640372327383123 0.0 64-65 0.0011971656438290322 1.7735787315985665E-4 4.433946828996416E-5 1.437552071163073 0.0 66-67 0.0012415051121189965 1.7735787315985665E-4 4.433946828996416E-5 1.498385821656904 0.0 68-69 0.0012415051121189965 1.7735787315985665E-4 4.433946828996416E-5 1.576046400366776 0.0 70-71 0.0012415051121189965 1.7735787315985665E-4 4.433946828996416E-5 1.6980242776324674 0.0 72-73 0.0012415051121189965 1.7735787315985665E-4 4.433946828996416E-5 1.8588435291201675 0.0 74-75 0.0012415051121189965 1.7735787315985665E-4 4.433946828996416E-5 2.0755083409190775 0.0 76-77 0.0012415051121189965 1.7735787315985665E-4 4.433946828996416E-5 2.359325277443138 0.0 78-79 0.0012415051121189965 1.7735787315985665E-4 4.433946828996416E-5 2.7171226168090037 0.0 80-81 0.0014188629852788532 1.7735787315985665E-4 4.433946828996416E-5 3.1407862363196113 0.0 82-83 0.0014188629852788532 1.7735787315985665E-4 4.433946828996416E-5 3.560304115545107 0.0 84-85 0.0014188629852788532 1.9952760730483873E-4 4.433946828996416E-5 3.9383867616536317 0.0 86-87 0.0014188629852788532 2.216973414498208E-4 4.433946828996416E-5 4.27527804172078 0.0 88-89 0.0014188629852788532 2.216973414498208E-4 4.433946828996416E-5 4.601062284981291 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 9970 0.0 27.207 1 TACATGG 10095 0.0 26.444258 2 ACATGGG 10255 0.0 25.200453 3 CGCATAG 425 0.0 22.906084 66-67 CATGGGA 7380 0.0 22.173618 4 GTATCAA 21560 0.0 21.902481 1 GAGTACT 9980 0.0 21.44067 12-13 AGTACTT 10260 0.0 20.993357 12-13 AGAGTAC 15800 0.0 20.07527 10-11 GGTATCA 16345 0.0 19.741457 1 ATGGGAG 3145 0.0 19.343388 5 GTACTTT 11095 0.0 19.073572 14-15 ATCAACG 24725 0.0 18.901697 3 TCAACGC 24775 0.0 18.882586 4 ACGCATA 635 0.0 18.779638 64-65 AACGCAG 24875 0.0 18.77647 6 CATGGGG 3420 0.0 18.753239 4 CAACGCA 24930 0.0 18.74627 5 TATCAAC 25095 0.0 18.644285 2 AACGCAT 705 0.0 17.260199 64-65 >>END_MODULE