FastQCFastQC Report
Fri 27 May 2016
SRR939599_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939599_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2176950
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156870.7205953283263281No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103890.4772273134431199No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT88840.4080938928317141No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80210.3684512735708216No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA76050.34934196926893135No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA59570.27363972530375064No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT46230.21236133122028525No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT35720.1640827763614231No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32730.15034796389443947No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT30700.1410229908817382No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC29780.13679689473805093No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29270.13445416752796344No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA26200.12035186843978962No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG26010.11947908771446289No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT25740.11823882036794597No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT23700.10886791152759595No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG1353.1286618E-1035.151692
GTACATG139750.026.2474841
TACATGG140900.025.327182
GAGTACT107000.023.9452912-13
ACATGGG142150.023.9360393
AGTACTT111850.022.6312512-13
CATGGGA101650.021.1942714
GTACTTT120550.021.176314-15
CATGGGG44300.020.8881954
GTCGGGA6850.020.7831172
GTATAGC6050.019.6085131
ACTTTTT139350.019.05452316-17
ATCACGC2251.6843114E-618.9819153
ATGGGAT36350.018.9288235
TACTTTT134300.018.84922414-15
AGTACAT103450.018.4864942
CCCTATA3409.0403773E-1018.1444762
ATGGGAG34850.017.8372765
GTATCAA340250.017.7398131
GATATAG6550.017.387211
Produced by FastQC (version 0.11.5)