FastQCFastQC Report
Fri 27 May 2016
SRR939599_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939599_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2176950
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158860.7297365580284343No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119340.5481981671604768No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT93920.4314292932772916No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92610.42541169985530214No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA75920.3487448035094972No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA65260.29977721123590345No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT51430.2362479615976481No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT42130.19352764188428764No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT40780.18732630515170307No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37690.1731321344082317No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC35890.16486368543145224No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31910.14658122602723994No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG31520.14478972874893775No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA31070.14272261650474288No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT27940.12834470245067642No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT26300.12081122671627736No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT25650.117825397919107No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC24780.11382898091366361No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC24470.11240497025655159No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23240.1067548634557523No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC22970.10551459610923539No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG134950.027.9077471
TACATGG135700.027.3674872
ACATGGG134050.025.9808123
CATGGGA97950.024.3602264
GAGTACT107700.022.45011712-13
AGTACTT111850.022.33141512-13
ATGGGAG34550.021.7946415
ATGGGAT35300.020.9316165
CATGGGG38650.020.4567954
GTACTTT120900.020.03762814-15
TACTTTT130800.019.2216214-15
GTCGGGA5350.018.4758012
GTATCAA313000.018.1088071
ACTTTTT137150.018.04020716-17
ATGGGAC20100.017.3266075
AGTACAT100050.017.2187542
GCTTACG4250.016.38806560-61
GTATATG7550.016.2119061
TCAACGC351800.016.0800154
AACGCAG352200.016.0381436
Produced by FastQC (version 0.11.5)