FastQCFastQC Report
Fri 27 May 2016
SRR939598_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939598_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2146756
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65680.30595000083847446No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44310.20640445397613888No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT37190.17323813232617027No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33190.15460536735427782No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA32660.15213652599550204No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA25580.11915653199525238No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG108000.034.6790921
TACATGG129900.027.275612
ACATGGG127850.026.6056843
CATGGGA92300.023.7469714
GAGTACT102500.023.64633212-13
AGTACTT110950.022.35592512-13
GTACTTT113450.021.44949214-15
CATGGGG40950.021.1100434
ATGGGAT34450.020.5498545
ATGGGAG33650.020.3371285
GTATCAA294350.020.249121
TTTGCGT5850.020.1895583
TACTTTT130750.018.90026314-15
ACTTTTT136050.018.86144416-17
CTTTGCG6500.018.1697542
GTCGGGA5750.017.2533662
TATCAAC347800.016.8156412
GACATAT8504.456524E-1016.582791
CATATAG4004.656613E-1016.5352483
AGTACAT91850.016.5100442
Produced by FastQC (version 0.11.5)