##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939598_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2146756 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 15.337873517064818 2.0 2.0 31.0 2.0 33.0 2 22.644763075076998 16.0 16.0 31.0 16.0 34.0 3 27.159856546342482 28.0 25.0 31.0 16.0 34.0 4 32.41975613437205 35.0 32.0 35.0 26.0 37.0 5 33.04832547341198 35.0 33.0 37.0 28.0 37.0 6 33.47457512637673 35.0 33.0 37.0 28.0 37.0 7 33.647433616116594 35.0 33.0 37.0 28.0 37.0 8 33.58028252861527 35.0 33.0 37.0 28.0 37.0 9 34.9378858146897 37.0 34.0 39.0 28.0 39.0 10-11 34.78802691130245 37.0 34.0 39.0 27.0 39.0 12-13 34.796738660565055 37.0 34.0 39.0 27.0 39.0 14-15 35.428405463872 38.0 34.0 40.0 27.0 41.0 16-17 35.07379529858075 38.0 33.0 40.0 26.0 41.0 18-19 35.39104537264598 38.0 34.0 40.0 27.0 41.0 20-21 35.4106889651176 38.0 34.0 40.0 27.0 41.0 22-23 35.226214809694255 38.0 34.0 40.0 26.0 41.0 24-25 35.00313915507864 38.0 34.0 40.0 25.0 41.0 26-27 35.05573036712137 38.0 34.0 40.0 25.0 41.0 28-29 34.79515510845201 38.0 33.5 40.0 24.5 41.0 30-31 34.63010281559711 38.0 33.0 40.0 24.0 41.0 32-33 34.27461830780955 38.0 33.0 40.0 24.0 41.0 34-35 33.982522000637246 38.0 33.0 40.0 23.0 41.0 36-37 33.655359062697386 38.0 32.5 40.0 20.5 41.0 38-39 33.414054974109774 37.0 32.0 40.0 19.0 41.0 40-41 33.005049712216945 37.0 31.0 40.0 16.5 41.0 42-43 32.710440310869046 37.0 31.0 40.0 15.0 41.0 44-45 32.303565239831634 37.0 30.5 40.0 13.5 41.0 46-47 31.864577995822536 36.5 30.0 39.0 9.0 41.0 48-49 31.414456044375793 36.0 30.0 39.0 8.0 40.0 50-51 30.712132398838058 35.5 28.5 38.5 6.5 40.0 52-53 30.718694159932475 35.5 28.5 38.5 2.0 40.0 54-55 30.828689660119736 36.0 29.0 39.0 2.0 40.0 56-57 30.548846957921626 35.0 28.0 39.0 2.0 40.0 58-59 30.145585478740948 35.0 28.0 38.5 2.0 40.0 60-61 29.52922269694367 34.5 26.5 38.0 2.0 40.0 62-63 29.14144527836419 34.0 26.0 38.0 2.0 40.0 64-65 28.54684859387839 34.0 26.0 37.5 2.0 39.5 66-67 27.947280221878966 33.0 25.0 37.0 2.0 39.0 68-69 27.621702000599974 33.0 24.5 36.0 2.0 39.0 70-71 27.088724335695346 33.0 23.5 36.0 2.0 39.0 72-73 26.493679067392847 32.0 22.0 35.5 2.0 38.0 74-75 25.948841181764486 32.0 20.5 35.0 2.0 37.5 76-77 25.320870886118406 31.0 19.0 35.0 2.0 37.0 78-79 24.663446148514318 31.0 16.5 34.0 2.0 36.0 80-81 23.93647345110483 30.0 9.5 34.0 2.0 36.0 82-83 23.263655487628775 30.0 6.5 34.0 2.0 35.0 84-85 22.588821924801884 29.0 2.0 34.0 2.0 35.0 86-87 21.918992656827324 29.0 2.0 34.0 2.0 35.0 88-89 21.111607933086013 28.5 2.0 33.0 2.0 35.0 90-91 20.15131319069331 26.5 2.0 33.0 2.0 34.0 92-93 19.12394235767828 25.5 2.0 32.0 2.0 34.0 94-95 18.13760879205648 24.5 2.0 32.0 2.0 34.0 96-97 17.220414662868066 21.5 2.0 31.5 2.0 34.0 98-99 16.171064853201763 10.0 2.0 31.0 2.0 34.0 100-101 14.598037923266547 2.0 2.0 30.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 15.041739676976434 1101 2 8.18017818900261 1101 3 3.8784262064823167 1101 4 2.541155658709812 1101 5 1.8378120630513735 1101 6 1.494721513441874 1101 7 1.0484947378438179 1101 8 0.9751789335684578 1101 9 1.1575670823368895 1101 10-11 1.1118768739760299 1101 12-13 0.8369848638451387 1101 14-15 1.0104602521011472 1101 16-17 1.1391860389026363 1101 18-19 1.0764168199332218 1101 20-21 1.0647738515203855 1101 22-23 1.013003327130356 1101 24-25 1.2184068698408552 1101 26-27 1.0613689945432867 1101 28-29 1.194613409173357 1101 30-31 1.2011555028136982 1101 32-33 1.2187814932028331 1101 34-35 1.1723182335459867 1101 36-37 1.190760905458795 1101 38-39 1.1075937757030587 1101 40-41 1.2274080814273205 1101 42-43 1.021869405277478 1101 44-45 1.0326427621848424 1101 46-47 1.1150844960807333 1101 48-49 0.7838478154055792 1101 50-51 0.9736471107067501 1101 52-53 1.1322113458481375 1101 54-55 1.2026562352676642 1101 56-57 1.0389854369304565 1101 58-59 1.0502048351191569 1101 60-61 1.0036758977693339 1101 62-63 0.8396704884462736 1101 64-65 0.8943886206192069 1101 66-67 0.6736859666959667 1101 68-69 0.5236437821677917 1101 70-71 0.29304860337248684 1101 72-73 0.5129230376824943 1101 74-75 0.2870841217519242 1101 76-77 0.272362563328695 1101 78-79 0.3583719972692947 1101 80-81 0.1795080465375385 1101 82-83 0.30438980410942307 1101 84-85 0.19064830099281238 1101 86-87 -0.07848487247406055 1101 88-89 -0.010755664467271941 1101 90-91 0.29663073852821853 1101 92-93 0.5039886398819604 1101 94-95 0.5000134179974509 1101 96-97 -0.12037652250435116 1101 98-99 -0.42857263454522965 1101 100-101 -0.6673651954217661 1102 1 14.719676065138884 1102 2 7.898233242081389 1102 3 3.6616932128807136 1102 4 2.1630464415054647 1102 5 1.5539291981412475 1102 6 1.1996225398727205 1102 7 0.9077029492906377 1102 8 0.7713140544557575 1102 9 1.0281501736199772 1102 10-11 0.8925463797800646 1102 12-13 0.5644964150684757 1102 14-15 0.6621489530924336 1102 16-17 0.850508718579718 1102 18-19 0.78999227242074 1102 20-21 0.7655225361797378 1102 22-23 0.5974579129918709 1102 24-25 0.5368559565964901 1102 26-27 0.793638512751329 1102 28-29 0.9223929901249548 1102 30-31 1.015051702021914 1102 32-33 0.731991234476105 1102 34-35 0.7836355381571778 1102 36-37 0.926375781987332 1102 38-39 0.7370176099856494 1102 40-41 0.7204013729316117 1102 42-43 0.6918484218471619 1102 44-45 0.6780972136697088 1102 46-47 0.5279449614373988 1102 48-49 0.41449892194557947 1102 50-51 0.30856984413449595 1102 52-53 0.442479789042995 1102 54-55 0.3971260160269985 1102 56-57 0.41434034632958827 1102 58-59 0.30414961665373497 1102 60-61 0.29273856897035344 1102 62-63 0.2004685823652146 1102 64-65 0.28564762643874886 1102 66-67 0.1782102216808248 1102 68-69 -0.13491011837281874 1102 70-71 0.17448446953334695 1102 72-73 -0.07404056691938976 1102 74-75 -0.283048290929095 1102 76-77 -0.22422561359366355 1102 78-79 -0.28373544202863954 1102 80-81 -0.3569799680991821 1102 82-83 -0.1848466264333517 1102 84-85 -0.3805549902013823 1102 86-87 -0.4686811657459238 1102 88-89 -0.6701896286817401 1102 90-91 -0.5889237718756384 1102 92-93 -0.3474968422717879 1102 94-95 -0.42999061330196753 1102 96-97 -1.0439136259640343 1102 98-99 -1.3561280132289593 1102 100-101 -1.3292845074133304 1103 1 14.929756065935408 1103 2 8.080234805258122 1103 3 3.8631259589441456 1103 4 2.268675968619611 1103 5 1.7080886919507705 1103 6 1.1772221177154378 1103 7 1.1201522082751723 1103 8 1.0259426008507475 1103 9 1.157592557613846 1103 10-11 0.949016587324131 1103 12-13 0.8074140794633919 1103 14-15 1.028935330554809 1103 16-17 1.041529214116693 1103 18-19 1.128069166776875 1103 20-21 1.006821366784152 1103 22-23 1.1694634090017857 1103 24-25 1.3754658245229905 1103 26-27 1.3120172893912923 1103 28-29 1.2625388534316144 1103 30-31 1.3601361085872554 1103 32-33 1.1828671610565848 1103 34-35 1.1251666548328885 1103 36-37 1.2791671476760982 1103 38-39 1.291160775070054 1103 40-41 1.0425108649987394 1103 42-43 1.2317339216405614 1103 44-45 1.0809004774234268 1103 46-47 0.9539431792167932 1103 48-49 0.8161264179256307 1103 50-51 0.7643753530879138 1103 52-53 0.8855393341286977 1103 54-55 0.8437702141023955 1103 56-57 0.6824273465735224 1103 58-59 0.736685696664491 1103 60-61 0.640919061122112 1103 62-63 0.41241921784135727 1103 64-65 0.5937227784004371 1103 66-67 0.6762446016124244 1103 68-69 0.6523897178421016 1103 70-71 0.5123720900697073 1103 72-73 0.4507851687444351 1103 74-75 0.28811617617200724 1103 76-77 0.25453200938204645 1103 78-79 0.185465993704387 1103 80-81 0.17003491194940779 1103 82-83 0.13828141964551222 1103 84-85 0.0936258106031076 1103 86-87 0.09593110170664687 1103 88-89 0.15090290242000393 1103 90-91 0.34125201083284296 1103 92-93 0.26329883459867176 1103 94-95 0.32261765162164124 1103 96-97 -0.34271933902651597 1103 98-99 -0.665728407507931 1103 100-101 -0.6114993396705266 1104 1 15.061236103525065 1104 2 8.14028010521066 1104 3 4.020686975126303 1104 4 2.3831356094050165 1104 5 1.7933166186698628 1104 6 1.4425352912822191 1104 7 1.1968412594989601 1104 8 1.1522919024304912 1104 9 1.184017661324404 1104 10-11 1.0581230721708081 1104 12-13 0.9540106899295253 1104 14-15 0.9894572221155826 1104 16-17 1.2402935960238324 1104 18-19 1.11151292498581 1104 20-21 1.2267458957702857 1104 22-23 1.1715359449003842 1104 24-25 1.2166418336807325 1104 26-27 1.238604325058894 1104 28-29 1.305543455957256 1104 30-31 1.2542528895943903 1104 32-33 1.2759389133117267 1104 34-35 1.3393895667068918 1104 36-37 1.3178105654652725 1104 38-39 1.2696038789288266 1104 40-41 1.3037883536185646 1104 42-43 0.969657810467254 1104 44-45 1.0280947145693773 1104 46-47 1.111442702250713 1104 48-49 1.1237944755462834 1104 50-51 1.1104050834483417 1104 52-53 1.1224614956286239 1104 54-55 1.3515681168706912 1104 56-57 1.2047466000782414 1104 58-59 1.0978812732573608 1104 60-61 0.9169030697500702 1104 62-63 0.9840202037815011 1104 64-65 0.8217907149268946 1104 66-67 0.9068990936869028 1104 68-69 0.9383706005683621 1104 70-71 1.110380047125414 1104 72-73 0.7796520037551637 1104 74-75 0.6420200844557655 1104 76-77 0.6231079463247653 1104 78-79 0.7293903326924642 1104 80-81 0.7172300163064342 1104 82-83 0.6430893166271439 1104 84-85 0.6317364848675915 1104 86-87 0.3361118087832473 1104 88-89 0.25083370291888585 1104 90-91 0.1438714218546231 1104 92-93 0.3546830729587285 1104 94-95 0.3166681895290111 1104 96-97 -0.09436546330259077 1104 98-99 -0.06373847346327466 1104 100-101 -0.03679610323883331 1105 1 14.772773978461519 1105 2 7.991459566318948 1105 3 3.7328427375133835 1105 4 2.2930229818190853 1105 5 1.5664690178720804 1105 6 1.1772889184379807 1105 7 0.8921109008895982 1105 8 0.9041551459485362 1105 9 1.0170179350720119 1105 10-11 0.8061406506777118 1105 12-13 0.7282499648894429 1105 14-15 0.7647566824417353 1105 16-17 1.0557513411257133 1105 18-19 0.9759535022021879 1105 20-21 0.9770276602691297 1105 22-23 0.8897631358588924 1105 24-25 1.0135197422490378 1105 26-27 1.013803965276324 1105 28-29 1.3085394279567808 1105 30-31 1.2247738899463485 1105 32-33 0.5789328322042238 1105 34-35 0.8936738144875847 1105 36-37 0.9313689910254865 1105 38-39 0.8966200300375036 1105 40-41 0.945498298477979 1105 42-43 1.0186764253043705 1105 44-45 1.149873976232982 1105 46-47 0.9007045613880109 1105 48-49 0.9243569291069633 1105 50-51 0.9763535211316565 1105 52-53 0.9403955811160891 1105 54-55 0.8428649961479984 1105 56-57 0.8449120218450865 1105 58-59 0.8920987461759005 1105 60-61 0.9056117631918639 1105 62-63 0.8375101924405293 1105 64-65 0.8695937926321058 1105 66-67 0.9309599438687499 1105 68-69 0.8175457403751771 1105 70-71 0.800001199993897 1105 72-73 0.7131652609605936 1105 74-75 0.5056317930318883 1105 76-77 0.35997490498887785 1105 78-79 0.41074782751081074 1105 80-81 0.4092924112069056 1105 82-83 0.41691161532488863 1105 84-85 0.3013859901665654 1105 86-87 0.16368826257856028 1105 88-89 0.2549517467967135 1105 90-91 0.5823171220618839 1105 92-93 0.6958862914484243 1105 94-95 0.7326171160473365 1105 96-97 0.3018525180043561 1105 98-99 -2.544485913489325E-4 1105 100-101 0.15185637417699027 1106 1 14.770452394204451 1106 2 7.897956016686365 1106 3 3.5056574380700205 1106 4 2.089498642165708 1106 5 1.4525467936272776 1106 6 1.033189931113185 1106 7 0.8322664907226098 1106 8 0.8856621829394413 1106 9 0.7387736858600178 1106 10-11 0.7862469959453406 1106 12-13 0.48445468431997085 1106 14-15 0.6535905251465479 1106 16-17 0.8946278177441229 1106 18-19 0.8073572597349781 1106 20-21 0.6558844965453261 1106 22-23 0.4372932430664136 1106 24-25 0.5935150477282107 1106 26-27 0.5228666898229193 1106 28-29 0.8942616656145006 1106 30-31 0.5641680093404702 1106 32-33 0.6460640967548485 1106 34-35 0.6086159952362138 1106 36-37 0.9095214575650417 1106 38-39 0.8238797711682153 1106 40-41 0.9860342403042424 1106 42-43 0.745268873832309 1106 44-45 0.694175306985926 1106 46-47 0.8789078053977022 1106 48-49 0.8992370272128625 1106 50-51 0.8428230498592484 1106 52-53 0.7516762272354462 1106 54-55 0.7385634737819622 1106 56-57 0.8151684959725252 1106 58-59 0.5336292557709257 1106 60-61 0.5823485921867544 1106 62-63 0.47870403782792437 1106 64-65 0.5847971420290001 1106 66-67 0.47976983493670744 1106 68-69 0.4891984991247078 1106 70-71 0.6608313637272865 1106 72-73 0.5032491874961345 1106 74-75 0.32164269581698335 1106 76-77 0.21100434634089993 1106 78-79 0.3583832261139541 1106 80-81 0.5891717907637073 1106 82-83 0.5952754311080604 1106 84-85 0.314908701108628 1106 86-87 0.2813292179614528 1106 88-89 0.17304548392271002 1106 90-91 0.586303249273822 1106 92-93 0.8715534403413798 1106 94-95 0.5030812987695796 1106 96-97 0.251096316925576 1106 98-99 -0.005049087256955076 1106 100-101 -0.04679026253416296 1107 1 14.6877482193246 1107 2 7.851121364003657 1107 3 3.5982074449315498 1107 4 1.999193705452683 1107 5 1.239119761273777 1107 6 0.942865759924878 1107 7 0.7127747173446792 1107 8 0.5872341204318374 1107 9 0.6053668792970655 1107 10-11 0.5564442121989188 1107 12-13 0.4469078788888936 1107 14-15 0.5495914742939263 1107 16-17 0.8006517779820257 1107 18-19 0.5656236453810308 1107 20-21 0.5817973665916583 1107 22-23 0.5389111028685463 1107 24-25 0.613250821521909 1107 26-27 0.5514805610606857 1107 28-29 0.5834772097337648 1107 30-31 0.48433106590572805 1107 32-33 0.6067096205410465 1107 34-35 0.6077805901896127 1107 36-37 0.4656075229644827 1107 38-39 0.2872476205208514 1107 40-41 0.6050819562287941 1107 42-43 0.39595915106397683 1107 44-45 0.6416997745331159 1107 46-47 0.39622419661066033 1107 48-49 0.5022491090525989 1107 50-51 0.33664314241406146 1107 52-53 0.3031086592979868 1107 54-55 0.38426573372183626 1107 56-57 0.4642654558527397 1107 58-59 0.447935137192605 1107 60-61 0.19533356451315598 1107 62-63 0.01226828966786897 1107 64-65 0.1347272506039161 1107 66-67 0.11184400677232631 1107 68-69 0.23021923573853087 1107 70-71 0.06787160705819417 1107 72-73 0.021130498947044885 1107 74-75 -0.1767213565762482 1107 76-77 -0.122754118215326 1107 78-79 0.046297021634462254 1107 80-81 -0.1785701632148502 1107 82-83 -0.08925901506296796 1107 84-85 0.02040280703658226 1107 86-87 -0.19604188423686608 1107 88-89 0.09756561150646448 1107 90-91 0.33663957517093124 1107 92-93 0.6079376512044945 1107 94-95 0.590587249435373 1107 96-97 -0.05989518698475749 1107 98-99 -0.1387089936220498 1107 100-101 -0.1923595456689675 1108 1 14.367565103544646 1108 2 7.476620076566213 1108 3 3.3896271380472562 1108 4 -0.15029985700100212 1108 5 0.33042413173832585 1108 6 0.5937855491876789 1108 7 0.6258144719693917 1108 8 0.6319273483546795 1108 9 0.7320607424550616 1108 10-11 0.6065956506079502 1108 12-13 0.36761471355593045 1108 14-15 0.390982610555092 1108 16-17 0.7618157126407823 1108 18-19 0.542074609195069 1108 20-21 0.5875626014177016 1108 22-23 -0.12147374406186628 1108 24-25 0.3582490628649424 1108 26-27 0.5558894126262715 1108 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64-65 0.3220726687020026 2312 66-67 0.24606156710004612 2312 68-69 0.21005256854285292 2312 70-71 0.2065181493786774 2312 72-73 -0.06628346155546083 2312 74-75 0.3030177238093401 2312 76-77 0.4280451140815309 2312 78-79 0.6037238124568702 2312 80-81 0.501862999388468 2312 82-83 0.36436368113107065 2312 84-85 0.3582999324171645 2312 86-87 0.3676357199424771 2312 88-89 0.8716232548397933 2312 90-91 1.0490206928053567 2312 92-93 1.4995446364284675 2312 94-95 1.4952778634462334 2312 96-97 1.280831627008407 2312 98-99 0.9926611561696426 2312 100-101 1.171727849335248 2313 1 -14.244368878681339 2313 2 -7.434330530814433 2313 3 -3.6915340962664374 2313 4 -1.1246959327655048 2313 5 -0.5749783241722781 2313 6 -0.9125937196536142 2313 7 -0.5143389013810946 2313 8 -0.2078491184437894 2313 9 -2.4544197483059733 2313 10-11 -0.5606141285397612 2313 12-13 -0.8400487430361281 2313 14-15 -0.9944315339852849 2313 16-17 -0.8937463171032931 2313 18-19 -0.16793429325277032 2313 20-21 -1.2314089114510196 2313 22-23 -2.112513484701317 2313 24-25 -1.2040379819504778 2313 26-27 -0.4298284222366533 2313 28-29 -0.10861573031777993 2313 30-31 -1.32111279385078 2313 32-33 -0.48789575380346406 2313 34-35 -1.3906262055587035 2313 36-37 -1.397975495333931 2313 38-39 -0.8080291599101912 2313 40-41 -0.5533767402478063 2313 42-43 -0.06846415385113147 2313 44-45 -0.18606838157504058 2313 46-47 -0.6879691943752988 2313 48-49 0.0358906524317284 2313 50-51 -0.7225464843355169 2313 52-53 0.058187300176467005 2313 54-55 -0.7186077682352128 2313 56-57 -0.2788275221929659 2313 58-59 -0.031279417948532284 2313 60-61 -1.3586372299377274 2313 62-63 -0.1593122885610576 2313 64-65 -0.007322541448406383 2313 66-67 -1.2425066061649659 2313 68-69 -0.18744715230428355 2313 70-71 0.11259354960117562 2313 72-73 -0.7388619655442454 2313 74-75 -0.17762354201449426 2313 76-77 0.3369394412817712 2313 78-79 0.3468121943214477 2313 80-81 0.12034529696128615 2313 82-83 0.1946309855069437 2313 84-85 0.4235141770708708 2313 86-87 0.39227044325029325 2313 88-89 0.7368441164502819 2313 90-91 1.0658370681191442 2313 92-93 1.4902002527773135 2313 94-95 1.4765715198524845 2313 96-97 1.2089931882793543 2313 98-99 1.2359035890592267 2313 100-101 1.2558262682961363 2314 1 -14.244368878681339 2314 2 -7.281387385302658 2314 3 -3.435976139790597 2314 4 -1.1481405713713286 2314 5 -1.1229198307949915 2314 6 -5.548726769608763 2314 7 -1.6199056264353615 2314 8 -4.147143936840266 2314 9 -1.4708803185836956 2314 10-11 -0.3003746384813084 2314 12-13 -2.723088868329377 2314 14-15 -2.8458212666758698 2314 16-17 -2.645215488938895 2314 18-19 -1.3528128971559 2314 20-21 -1.9425973391544389 2314 22-23 -1.1472494948171885 2314 24-25 -3.09116924061032 2314 26-27 -4.705627354557848 2314 28-29 -1.9248051879811996 2314 30-31 -2.020536712166603 2314 32-33 -4.039186631211088 2314 34-35 -2.6763539001393752 2314 36-37 -0.986752132500591 2314 38-39 -1.4774340065679397 2314 40-41 -2.367235304469638 2314 42-43 -1.3278299177021822 2314 44-45 -2.489849430867679 2314 46-47 -2.0984382680863334 2314 48-49 -0.9656314962318646 2314 50-51 -1.9438695793096556 2314 52-53 -0.7432538957184498 2314 54-55 -0.17801560038513387 2314 56-57 -0.7925754005101489 2314 58-59 -1.311382782786115 2314 60-61 -0.11643247876006058 2314 62-63 -2.629303916656415 2314 64-65 -0.93911570181805 2314 66-67 -2.039516277350234 2314 68-69 -1.981009502116283 2314 70-71 -2.4998001400844068 2314 72-73 -2.4067880714811984 2314 74-75 -1.9038954904274163 2314 76-77 -0.5311674817892644 2314 78-79 -0.16341642200383788 2314 80-81 0.02575277836807288 2314 82-83 -0.11783026050804679 2314 84-85 0.16835550407691002 2314 86-87 0.2910770466857677 2314 88-89 0.5470329262131877 2314 90-91 0.9274718582195582 2314 92-93 1.2653782514397562 2314 94-95 1.2417286339880604 2314 96-97 1.2912742922016243 2314 98-99 1.380504406485592 2314 100-101 1.4350553153952337 2315 1 -14.244368878681339 2315 2 -7.422955852797362 2315 3 -4.550710671317923 2315 4 -2.8692831719338265 2315 5 -3.5500189744071733 2315 6 -2.816574206133673 2315 7 -2.936355532973522 2315 8 -3.5710178948418125 2315 9 -2.505367104580742 2315 10-11 -4.753271528148979 2315 12-13 -4.498573665447125 2315 14-15 -3.357069535464241 2315 16-17 -4.548520899149924 2315 18-19 -1.4335278183440465 2315 20-21 -3.3435314412148713 2315 22-23 -4.175344306550208 2315 24-25 -5.1404615246808945 2315 26-27 -4.742961526338547 2315 28-29 -2.576047609952276 2315 30-31 -3.131282001552904 2315 32-33 -4.458203404490774 2315 34-35 -3.6066869532978743 2315 36-37 -4.746526545766958 2315 38-39 -3.215510122372663 2315 40-41 -4.328520080258258 2315 42-43 -3.073917811176159 2315 44-45 -1.540735524575922 2315 46-47 -0.9540091508649482 2315 48-49 -0.5522693466113822 2315 50-51 -0.4196148126135135 2315 52-53 -0.17652647010379852 2315 54-55 -1.9827262051835497 2315 56-57 -3.977397675246113 2315 58-59 -3.164071651097597 2315 60-61 -3.9868896005271495 2315 62-63 -1.9047872894581381 2315 64-65 -2.7074450022598917 2315 66-67 -1.3639727093922893 2315 68-69 -0.7402941287077986 2315 70-71 -1.634504294511597 2315 72-73 -1.671062967323639 2315 74-75 -1.944646685384054 2315 76-77 -1.7820668038326382 2315 78-79 -1.1106821892268002 2315 80-81 -0.8571870490867255 2315 82-83 -0.4479777252878314 2315 84-85 -0.3402251098068625 2315 86-87 -0.9870898265363977 2315 88-89 -1.5897487711833946 2315 90-91 -0.8759187616979709 2315 92-93 0.19151847428468471 2315 94-95 0.3572397738307913 2315 96-97 -0.585139141163797 2315 98-99 -0.1807115392308738 2315 100-101 0.34009696684694113 2316 1 -14.244368878681339 2316 2 -7.350562225448607 2316 3 -5.030155958705652 2316 4 -2.8836989403265676 2316 5 -2.0516106990211966 2316 6 -1.159862320153941 2316 7 -5.094108134312485 2316 8 -3.042721367368653 2316 9 -1.326332782007519 2316 10-11 -4.528193955394187 2316 12-13 -3.6323018138403 2316 14-15 -1.986275632700746 2316 16-17 -3.382350260662335 2316 18-19 -2.5180938064213905 2316 20-21 -3.5663756736850445 2316 22-23 -3.2407476668976756 2316 24-25 -3.3903655250260627 2316 26-27 -4.123748651598323 2316 28-29 -3.711164427509786 2316 30-31 -2.737292137246797 2316 32-33 -2.8894118316065445 2316 34-35 -4.198987295588779 2316 36-37 -2.56024676609875 2316 38-39 -3.508868887824292 2316 40-41 -3.005781735047819 2316 42-43 -7.026121488646083 2316 44-45 -3.786102462582434 2316 46-47 -4.8730610374199586 2316 48-49 -4.645182091194773 2316 50-51 -3.6145609880879377 2316 52-53 -4.58980929874981 2316 54-55 -4.700818185077555 2316 56-57 -4.577148417715353 2316 58-59 -3.4528938036471573 2316 60-61 -3.3541582125950633 2316 62-63 -2.5695618880213793 2316 64-65 -2.8573018117634987 2316 66-67 -3.4088384357177652 2316 68-69 -3.1429566809323326 2316 70-71 -3.128148362308206 2316 72-73 -3.9785059068546396 2316 74-75 -4.355307860534886 2316 76-77 -4.075178289274486 2316 78-79 -4.494450067814203 2316 80-81 -4.368323997807806 2316 82-83 -3.8293381518570833 2316 84-85 -3.462367502103877 2316 86-87 -3.6185416685073015 2316 88-89 -3.5673587393273287 2316 90-91 -2.598776657040368 2316 92-93 -2.6002591732563616 2316 94-95 -1.7769321482476936 2316 96-97 -2.0648167872666434 2316 98-99 -1.9868045510312822 2316 100-101 -1.581865920477508 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14270.0 3 3990.0 4 4403.0 5 5517.0 6 6548.0 7 8090.0 8 10553.0 9 12828.0 10 15608.0 11 18694.0 12 22316.0 13 25243.0 14 27729.0 15 31469.0 16 35435.0 17 37521.0 18 32519.0 19 31056.0 20 32838.0 21 36253.0 22 39694.0 23 44442.0 24 49549.0 25 55272.0 26 62131.0 27 70138.0 28 78935.0 29 89616.0 30 102054.0 31 117049.0 32 133985.0 33 155129.0 34 180264.0 35 206015.0 36 211500.0 37 122653.0 38 15368.0 39 82.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.893077533334456 18.706913927642706 13.767883543615046 21.632124995407793 2 15.280669012393625 21.620658932636733 40.02162345627497 23.077048598694667 3 19.587451935072416 27.21945122619489 30.19510454777578 22.997992290956915 4 12.137122876386202 16.611897604489428 38.54673198816596 32.704247530958405 5 12.747204059360687 38.99896463916276 33.64395713046479 14.60987417101176 6 29.457292016053078 37.09631145915894 18.37459572250738 15.071800802280608 7 27.07404919195312 34.50570111596312 21.474732206173254 16.9455174859105 8 26.201847154647535 33.084613902504906 20.544405375661356 20.169133567186204 9 27.768992582185113 16.607844728742705 20.80332761440424 34.81983507466794 10-11 24.7220900729464 26.48189865860712 27.586215742243613 21.209795526202868 12-13 27.187318006778167 24.533753336080796 27.59013161469926 20.68879704244178 14-15 23.805117580829165 25.08860634896125 27.63718035529309 23.469095714916495 16-17 21.48192082897668 28.653647589284486 27.83121084696617 22.033220734772662 18-19 21.237734627333502 27.44264224590062 30.660218591700684 20.6594045350652 20-21 22.851381175379746 25.952979863745522 29.99749647161155 21.198142489263176 22-23 22.225956014065194 26.07899583402175 29.9141834617291 21.780864690183954 24-25 22.2075162162894 26.19594842533691 30.372967653322952 21.22356770505074 26-27 22.262957469181117 25.81587912158448 30.884075713563806 21.037087695670593 28-29 21.51097884755653 26.48766739606127 30.837660102115244 21.163693654266957 30-31 21.578367940358326 26.560000709664227 30.507834786433087 21.35379656354436 32-33 21.544304967528785 26.64875859631727 30.26146436289332 21.545472073260626 34-35 22.19081082405714 26.17394622512816 30.18634845024698 21.448894500567718 36-37 22.409925440650632 25.901457327134487 30.44406812014344 21.244549112071446 38-39 22.153921327730185 26.13492775202785 30.540743805374888 21.170407114867075 40-41 21.803577183107727 26.358606434486642 30.609069455894716 21.228746926510915 42-43 21.549080023006717 26.929478347203084 29.612327493376235 21.90911413641396 44-45 21.800477703476126 26.707288186815433 29.399556834928596 22.092677274779845 46-47 22.20021480381586 26.91254646165453 28.742744068559812 22.1444946659698 48-49 22.394387962814257 26.825252019705903 28.66684470207704 22.1135153154028 50-51 22.082156575675487 27.255843554597625 28.89793848634315 21.764061383383734 52-53 22.15915313993653 27.581475064553956 28.36469311504674 21.894678680462775 54-55 21.42330435139964 27.180183007415003 28.733747280900594 22.662765360284755 56-57 22.192985056752963 27.614227844307116 27.87591500046338 22.31687209847654 58-59 22.473004971994612 26.953400267500214 28.128590509062434 22.44500425144274 60-61 22.207875672431317 26.912841326277725 28.87401183049737 22.005271170793588 62-63 22.498666282070488 26.821639322531972 28.535869427731157 22.143824967666383 64-65 22.099006093551438 27.35592510023302 28.519822826386754 22.025245979828792 66-67 21.89040387468781 27.2635600771241 28.148732264467885 22.69730378372021 68-69 21.638608745241914 27.498536377295217 28.463068989889162 22.39978588757371 70-71 22.1089622072393 27.41331618213841 27.96866857548389 22.5090530351384 72-73 22.100982524724177 27.10839703494285 27.93569905188103 22.85492138845194 74-75 21.721425447603444 28.074651982031185 27.65473516684245 22.54918740352292 76-77 22.148764884684123 27.187317144699747 27.903070871620578 22.760847098995555 78-79 21.911390998263833 27.343849959234145 28.130472220847274 22.614286821654748 80-81 21.964869825675375 27.92238737803709 27.662694848201753 22.45004794808578 82-83 21.898703707983977 27.591994521874565 27.665840885483973 22.84346088465749 84-85 21.820476350299476 27.38353700577011 27.532399391853506 23.26358725207691 86-87 21.96061853677575 27.3849533828535 27.651873952800692 23.00255412757006 88-89 22.134377294909495 27.012132295098574 27.649326390811446 23.204164019180485 90-91 21.80668191245482 27.032238820183256 27.93531603267808 23.225763234683843 92-93 21.83977689206577 27.553031662739002 27.807643393270208 22.799548051925015 94-95 21.83208413234954 27.354361128317894 27.495504767713058 23.318049971619505 96-97 21.608833949578813 27.13493381988349 27.470184439223623 23.78604779131408 98-99 21.933782049769633 27.211203131748878 26.908386113539912 23.94662870494157 100-101 22.044443946117113 27.095801249618077 27.29496088286162 23.56479392140319 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 5264.0 1 3728.5 2 2076.0 3 1745.5 4 1569.5 5 1682.5 6 2295.0 7 4945.0 8 6665.5 9 5609.0 10 4568.0 11 4032.0 12 3978.0 13 4100.5 14 4189.5 15 4341.0 16 4417.0 17 4621.5 18 4955.0 19 5301.5 20 5767.5 21 6520.0 22 7467.0 23 8436.5 24 9587.5 25 11196.5 26 13576.0 27 16221.0 28 18868.5 29 21902.0 30 25382.0 31 29190.0 32 33241.0 33 37842.5 34 42164.0 35 45305.0 36 49535.0 37 53650.0 38 55266.5 39 56088.0 40 57581.5 41 59474.0 42 61359.5 43 64286.5 44 67786.5 45 70955.5 46 76766.5 47 94565.0 48 108027.0 49 97639.5 50 81046.0 51 71995.0 52 68524.0 53 66501.5 54 63465.0 55 59479.5 56 54640.5 57 50041.0 58 46233.0 59 41676.0 60 36130.0 61 30464.5 62 24655.5 63 18788.5 64 13997.0 65 10242.5 66 7346.0 67 5452.5 68 3738.0 69 2494.0 70 1768.5 71 1289.0 72 976.5 73 763.0 74 565.5 75 428.5 76 315.5 77 196.5 78 114.5 79 82.0 80 60.0 81 42.0 82 33.0 83 24.5 84 18.0 85 13.5 86 7.0 87 4.0 88 6.0 89 7.5 90 5.5 91 4.5 92 4.0 93 2.5 94 2.0 95 3.0 96 3.0 97 2.0 98 1.0 99 1.5 100 1.5 >>END_MODULE >>Per base N content fail #Base N-Count 1 53.0854461336081 2 0.0867821028565892 3 0.11743300123535233 4 0.19205722494778169 5 0.11999500641898754 6 0.1451958210434721 7 0.15446562161698862 8 0.15106514200961824 9 0.1396525734643341 10-11 0.1265164741591499 12-13 0.13790575174821917 14-15 0.14652340554771945 16-17 0.13995535589512734 18-19 0.14768795335846271 20-21 0.17610291994059876 22-23 0.19387391953254118 24-25 0.22238204993953667 26-27 0.2144398338702675 28-29 0.2128094669352269 30-31 0.22815820708082332 32-33 0.2191678979818852 34-35 0.20489054182217262 36-37 0.19100913191811272 38-39 0.18779497996046127 40-41 0.18802788952260993 42-43 0.18183249516945568 44-45 0.19627288802267234 46-47 0.2236630525313543 48-49 0.22638809440849356 50-51 0.23763762626027363 52-53 0.26633208431698807 54-55 0.2525671291940025 56-57 0.22757593317545172 58-59 0.1841848817471571 60-61 0.19368759188282228 62-63 0.19792654591392783 64-65 0.186281067806495 66-67 0.1972743991399116 68-69 0.18430133652823144 70-71 0.19762376348313454 72-73 0.21765398582791898 74-75 0.25058739791573886 76-77 0.25047094313466456 78-79 0.24492769555552657 80-81 0.25049423409087945 82-83 0.23978039423204126 84-85 0.21003784314565793 86-87 0.2204721915299177 88-89 0.22298761480112317 90-91 0.21078315374453363 92-93 0.20843076716683218 94-95 0.24783906508238476 96-97 0.2780707262492803 98-99 0.29600476253472685 100-101 0.2916959356349767 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2146756.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.745495342334124 #Duplication Level Percentage of deduplicated Percentage of total 1 79.37412088364837 50.597426530904734 2 11.737115629183835 14.963764993451505 3 4.07362622714974 7.790259650675519 4 1.834841483214572 4.6785151688870386 5 0.9300493817357708 2.9643229265789155 6 0.5489654279170197 2.0996443877032105 7 0.33714468444171897 1.5044018438240614 8 0.23286749394331097 1.187540300043548 9 0.1697527041918975 0.9738873192971904 >10 0.7144696726081623 7.642865446824176 >50 0.031033544205471407 1.3636550238090077 >100 0.01305482147728732 1.46867467471397 >500 0.0015660245026583193 0.7004528369419787 >1k 0.0013050204188819314 1.7003312373558281 >5k 8.700136125879544E-5 0.3642576589893043 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6568 0.30595000083847446 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4431 0.20640445397613888 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3719 0.17323813232617027 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3319 0.15460536735427782 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3266 0.15213652599550204 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2558 0.11915653199525238 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 4.658191242973118E-5 0.0 0.0 0.0 0.0 9 4.658191242973118E-5 0.0 0.0 0.0 4.658191242973118E-5 10-11 4.658191242973118E-5 0.0 0.0 0.0 4.658191242973118E-5 12-13 4.658191242973118E-5 0.0 0.0 0.0 4.658191242973118E-5 14-15 4.658191242973118E-5 0.0 0.0 0.0 9.316382485946237E-5 16-17 4.658191242973118E-5 0.0 0.0 1.6303669350405914E-4 9.316382485946237E-5 18-19 6.987286864459677E-5 0.0 0.0 2.794914745783871E-4 1.3974573728919355E-4 20-21 9.316382485946237E-5 0.0 0.0 7.453105988756989E-4 1.3974573728919355E-4 22-23 9.316382485946237E-5 0.0 0.0 0.0013508754604622045 1.8632764971892473E-4 24-25 9.316382485946237E-5 0.0 0.0 0.0027250418771392745 1.8632764971892473E-4 26-27 9.316382485946237E-5 0.0 0.0 0.004262244987320403 1.8632764971892473E-4 28-29 1.6303669350405914E-4 0.0 0.0 0.008105252762773227 1.8632764971892473E-4 30-31 1.8632764971892473E-4 0.0 0.0 0.018236818716239757 1.8632764971892473E-4 32-33 1.8632764971892473E-4 0.0 0.0 0.037894385761586324 1.8632764971892473E-4 34-35 1.8632764971892473E-4 0.0 0.0 0.06891793943978729 1.8632764971892473E-4 36-37 1.8632764971892473E-4 0.0 0.0 0.11552314282573334 1.8632764971892473E-4 38-39 1.8632764971892473E-4 0.0 0.0 0.1846739918276693 1.8632764971892473E-4 40-41 1.8632764971892473E-4 0.0 0.0 0.263048059490692 1.8632764971892473E-4 42-43 2.3290956214865591E-4 0.0 0.0 0.3406069436861944 1.8632764971892473E-4 44-45 2.3290956214865591E-4 0.0 0.0 0.430277125113427 1.8632764971892473E-4 46-47 2.3290956214865591E-4 0.0 0.0 0.5369497045775113 2.5620051836352153E-4 48-49 3.260733870081183E-4 0.0 0.0 0.6527057569653933 3.260733870081183E-4 50-51 3.260733870081183E-4 0.0 0.0 0.7838571314113015 3.260733870081183E-4 52-53 3.260733870081183E-4 0.0 0.0 0.9098844954899392 3.493643432229839E-4 54-55 3.260733870081183E-4 0.0 0.0 1.0429923102578962 3.9594625565271503E-4 56-57 3.7265529943784946E-4 0.0 0.0 1.1969688217943726 4.4252816808244627E-4 58-59 3.7265529943784946E-4 0.0 0.0 1.3731881965160455 5.124010367270431E-4 60-61 3.7265529943784946E-4 0.0 0.0 1.5867196830939334 5.124010367270431E-4 62-63 3.7265529943784946E-4 0.0 0.0 1.8006936978399035 5.124010367270431E-4 64-65 3.7265529943784946E-4 0.0 0.0 2.0033250169092343 5.124010367270431E-4 66-67 3.7265529943784946E-4 0.0 0.0 2.233346500487247 5.124010367270431E-4 68-69 4.1923721186758065E-4 0.0 0.0 2.4966041785838726 5.124010367270431E-4 70-71 4.1923721186758065E-4 0.0 0.0 2.7956600563827467 5.589829491567742E-4 72-73 4.1923721186758065E-4 0.0 0.0 3.1041953533610713 6.521467740162366E-4 74-75 4.1923721186758065E-4 0.0 0.0 3.4156886017786836 6.521467740162366E-4 76-77 4.1923721186758065E-4 0.0 0.0 3.7392698564718114 6.521467740162366E-4 78-79 4.1923721186758065E-4 0.0 0.0 4.118400041737393 6.521467740162366E-4 80-81 4.891100805121775E-4 0.0 0.0 4.543390119790046 6.521467740162366E-4 82-83 5.124010367270431E-4 0.0 0.0 4.966726539951443 6.521467740162366E-4 84-85 5.822739053716398E-4 0.0 0.0 5.413889608320647 6.521467740162366E-4 86-87 6.754377302311022E-4 0.0 0.0 5.871440443161681 6.521467740162366E-4 88-89 6.987286864459678E-4 0.0 0.0 6.3538660192401935 6.521467740162366E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 10800 0.0 34.679092 1 TACATGG 12990 0.0 27.27561 2 ACATGGG 12785 0.0 26.605684 3 CATGGGA 9230 0.0 23.746971 4 GAGTACT 10250 0.0 23.646332 12-13 AGTACTT 11095 0.0 22.355925 12-13 GTACTTT 11345 0.0 21.449492 14-15 CATGGGG 4095 0.0 21.110043 4 ATGGGAT 3445 0.0 20.549854 5 ATGGGAG 3365 0.0 20.337128 5 GTATCAA 29435 0.0 20.24912 1 TTTGCGT 585 0.0 20.189558 3 TACTTTT 13075 0.0 18.900263 14-15 ACTTTTT 13605 0.0 18.861444 16-17 CTTTGCG 650 0.0 18.169754 2 GTCGGGA 575 0.0 17.253366 2 TATCAAC 34780 0.0 16.815641 2 GACATAT 850 4.456524E-10 16.58279 1 CATATAG 400 4.656613E-10 16.535248 3 AGTACAT 9185 0.0 16.510044 2 >>END_MODULE