##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939598_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2146756 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.46305355615636 31.0 31.0 34.0 30.0 34.0 2 31.654718095582357 31.0 31.0 34.0 30.0 34.0 3 31.697462590066127 31.0 31.0 34.0 30.0 34.0 4 35.50131407574964 37.0 35.0 37.0 33.0 37.0 5 35.26638844843103 37.0 35.0 37.0 33.0 37.0 6 35.28356646027774 37.0 35.0 37.0 32.0 37.0 7 35.194201856196045 37.0 35.0 37.0 32.0 37.0 8 35.157237245406556 37.0 35.0 37.0 32.0 37.0 9 36.731470181054576 39.0 35.0 39.0 32.0 39.0 10-11 36.61362353243685 39.0 35.0 39.0 32.0 39.0 12-13 36.5453945394819 39.0 35.0 39.0 32.0 39.0 14-15 37.53802691130245 39.0 36.0 41.0 32.0 41.0 16-17 37.510942557048864 39.0 36.0 41.0 32.0 41.0 18-19 37.539650756769745 39.0 36.0 41.0 32.0 41.0 20-21 37.49880121448362 39.0 36.0 41.0 32.0 41.0 22-23 37.35036119614898 39.0 36.0 41.0 31.5 41.0 24-25 37.19946095410937 39.0 36.0 40.5 31.0 41.0 26-27 36.87833712820647 39.0 36.0 40.0 30.5 41.0 28-29 36.671236740458625 38.5 35.0 40.0 30.0 41.0 30-31 36.396013100697054 38.0 35.0 40.0 30.0 41.0 32-33 36.16867357072718 38.0 35.0 40.0 30.0 41.0 34-35 36.08969976094163 38.0 35.0 40.0 30.0 41.0 36-37 35.947267877672175 38.0 35.0 40.0 30.0 41.0 38-39 35.93729655349746 38.0 35.0 40.0 29.5 41.0 40-41 35.80134933825735 38.0 35.0 40.0 29.0 41.0 42-43 35.575256806083225 38.0 34.5 40.0 27.5 41.0 44-45 35.29712156388523 38.0 34.0 40.0 27.0 41.0 46-47 34.89300414206365 38.0 34.0 40.0 25.5 41.0 48-49 34.50639243584273 38.0 33.0 40.0 24.0 41.0 50-51 34.22896407416586 38.0 33.0 40.0 23.5 41.0 52-53 33.96430451341466 38.0 33.0 40.0 23.0 41.0 54-55 33.672649569862614 37.0 33.0 40.0 22.5 41.0 56-57 32.88213308825036 36.5 32.0 40.0 17.0 41.0 58-59 29.854201176100126 34.5 25.5 39.0 7.5 40.0 60-61 29.551151132219964 34.0 25.5 38.0 7.5 40.0 62-63 31.290180858933198 35.0 29.5 38.5 8.0 40.0 64-65 31.30535119035419 35.0 30.5 38.5 4.5 40.0 66-67 26.543782805311828 31.5 18.0 36.5 2.0 39.5 68-69 19.24374894026149 19.0 7.5 31.5 2.0 36.5 70-71 17.333374635962354 14.5 7.5 29.0 2.0 35.0 72-73 19.00817535854098 18.5 7.5 30.5 2.0 35.0 74-75 20.42066145384012 22.5 7.5 32.0 2.0 35.5 76-77 22.175607521301906 27.0 11.0 33.0 2.0 35.5 78-79 24.304105124196695 30.5 12.0 34.5 2.0 36.0 80-81 25.850783694094716 31.5 20.5 35.0 2.0 36.0 82-83 26.940885456940613 33.0 25.5 35.0 2.0 36.0 84-85 26.990528965564785 33.0 26.0 35.0 2.0 36.0 86-87 26.72470648736978 33.0 25.5 35.0 2.0 35.5 88-89 26.28077760118057 33.0 24.5 35.0 2.0 35.0 90-91 24.51152180312993 32.0 11.5 34.5 2.0 35.0 92-93 22.968086731794394 31.0 2.0 34.0 2.0 35.0 94-95 19.33056085554204 26.5 2.0 34.0 2.0 35.0 96-97 16.709704782471782 14.0 2.0 32.5 2.0 35.0 98-99 16.966879794443336 19.0 2.0 33.0 2.0 35.0 100-101 16.201324230606552 2.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.36397193833317587 1101 2 0.3524287518270377 1101 3 0.28986294519350864 1101 4 0.2856823691134025 1101 5 0.3683393608371972 1101 6 0.3532253645878569 1101 7 0.34729357063299915 1101 8 0.46451492704420616 1101 9 0.4619231294390005 1101 10-11 0.4120121841822808 1101 12-13 0.3446931815608991 1101 14-15 0.5250073283768728 1101 16-17 0.5258827146353227 1101 18-19 0.4726858451121245 1101 20-21 0.5655014722167238 1101 22-23 0.48124613244199566 1101 24-25 0.3617803618913129 1101 26-27 0.5092286857487807 1101 28-29 0.45554764561045147 1101 30-31 0.49135098092045126 1101 32-33 0.36981604629105647 1101 34-35 0.21677574705457658 1101 36-37 0.12461007951185366 1101 38-39 0.22808387701511634 1101 40-41 0.20550429080554267 1101 42-43 0.4182813082120518 1101 44-45 0.39353411287415696 1101 46-47 0.030221094907005863 1101 48-49 -0.004672376235994591 1101 50-51 -0.026343478742170134 1101 52-53 -0.21272910312851678 1101 54-55 -0.07472212169148662 1101 56-57 -3.2874457956312106 1101 58-59 -10.20980222603637 1101 60-61 -11.476892869905509 1101 62-63 -3.9640453924775585 1101 64-65 -1.3668796319024743 1101 66-67 -2.0162774633326705 1101 68-69 -4.725980125254651 1101 70-71 -4.606012789267108 1101 72-73 -3.5350649635598135 1101 74-75 -6.306279219516247 1101 76-77 -4.950882160033721 1101 78-79 0.9231465677008579 1101 80-81 1.6196029023530727 1101 82-83 0.2835189559331859 1101 84-85 0.0066873398117905936 1101 86-87 -0.04753319204898432 1101 88-89 -0.12433288900839301 1101 90-91 1.2197245073520868 1101 92-93 0.8139468570191148 1101 94-95 -8.301811622073611 1101 96-97 -9.2469813749675 1101 98-99 -8.317837003132047 1101 100-101 -9.377205519270612 1102 1 0.1761276498575235 1102 2 0.25796899763293624 1102 3 0.20510497820881923 1102 4 0.18527944590448442 1102 5 0.23329393119370678 1102 6 0.2056483822738855 1102 7 0.19530117841857475 1102 8 0.30741932322791143 1102 9 0.18671830841835657 1102 10-11 0.24727799152751828 1102 12-13 0.24936142629177738 1102 14-15 0.27810770773248805 1102 16-17 0.11032841096476886 1102 18-19 0.12480227730877402 1102 20-21 0.1256716137256646 1102 22-23 0.16158269426041727 1102 24-25 0.15190863990626013 1102 26-27 -0.13616467604484228 1102 28-29 0.14375702510044164 1102 30-31 0.1840438889344682 1102 32-33 -0.03649510800434541 1102 34-35 0.12812220106159344 1102 36-37 -0.0862876598594724 1102 38-39 -0.008879830953425483 1102 40-41 -0.017535619058740792 1102 42-43 0.06854923457364492 1102 44-45 -0.003317545643774622 1102 46-47 -0.026265162496628136 1102 48-49 0.029307033126961812 1102 50-51 0.15268611513025832 1102 52-53 -0.1494746040949977 1102 54-55 -0.4020065763807139 1102 56-57 -3.8079943624525825 1102 58-59 -10.030361129522927 1102 60-61 -7.863187795636566 1102 62-63 -2.4168351550443568 1102 64-65 -0.8055269758963561 1102 66-67 -2.235441950685761 1102 68-69 -5.166084215473067 1102 70-71 -4.913184987772427 1102 72-73 -3.631681049478173 1102 74-75 -4.016703332888792 1102 76-77 -4.111213656886207 1102 78-79 1.4778447887607769 1102 80-81 1.7738929285048357 1102 82-83 0.40554380527629164 1102 84-85 0.06296159221248132 1102 86-87 0.050820487286987515 1102 88-89 -0.16747451165809224 1102 90-91 1.2472274791433264 1102 92-93 0.536367807372109 1102 94-95 -8.750056249591882 1102 96-97 -9.72047754523305 1102 98-99 -8.818112785359183 1102 100-101 -9.795073065242931 1103 1 0.23508191449239035 1103 2 0.22132156368213174 1103 3 0.34925396786895035 1103 4 0.24104642304924795 1103 5 0.2219146019657856 1103 6 0.30480277335681194 1103 7 0.22788992852436252 1103 8 0.3094624051752106 1103 9 0.4298273375063886 1103 10-11 0.3616695722775418 1103 12-13 0.3934921954842565 1103 14-15 0.4029062620429258 1103 16-17 0.40379204221436993 1103 18-19 0.48583536786847503 1103 20-21 0.40665293027891636 1103 22-23 0.3070313110481351 1103 24-25 0.41430488031686963 1103 26-27 0.4503050834549498 1103 28-29 0.31469667353502473 1103 30-31 0.4455332877088125 1103 32-33 0.2580460199720349 1103 34-35 0.2081003962396366 1103 36-37 0.16300009907278934 1103 38-39 0.22233033666492474 1103 40-41 0.22380990771689113 1103 42-43 0.25957879056175415 1103 44-45 0.47208641606336954 1103 46-47 0.40038451167560396 1103 48-49 0.1294562816571201 1103 50-51 0.015094402953899078 1103 52-53 0.2755776428470966 1103 54-55 0.17739324340591622 1103 56-57 0.5716782665219426 1103 58-59 -8.202719385734564 1103 60-61 -7.438011788905946 1103 62-63 -2.11856639816812 1103 64-65 -0.4707325535498974 1103 66-67 -2.0458386690102763 1103 68-69 -3.589739261198474 1103 70-71 -4.072657092037311 1103 72-73 -3.0628441226637193 1103 74-75 -3.853935514416424 1103 76-77 -0.5216660322676638 1103 78-79 3.176427375768487 1103 80-81 2.6756030637644272 1103 82-83 1.226267347016595 1103 84-85 0.7882032125397522 1103 86-87 0.7035459406829148 1103 88-89 0.42998457940939616 1103 90-91 1.9824809975355429 1103 92-93 1.686852840203798 1103 94-95 -7.167289625904278 1103 96-97 -8.818674714588813 1103 98-99 -8.020248064864145 1103 100-101 -9.242971189928678 1104 1 0.16364954197636195 1104 2 0.2524723764165735 1104 3 0.17471571834236954 1104 4 0.12348729108022383 1104 5 0.24931216462967143 1104 6 0.2075834856366754 1104 7 0.2332615126982347 1104 8 0.21486564255295093 1104 9 0.16679827962872196 1104 10-11 0.15837509656969928 1104 12-13 0.2839125878736084 1104 14-15 0.3529369691983604 1104 16-17 0.2714298144595091 1104 18-19 0.38529200100823857 1104 20-21 0.2515754883308077 1104 22-23 0.37493738657119025 1104 24-25 0.38769115819967936 1104 26-27 0.3574509223397442 1104 28-29 0.3325727514389598 1104 30-31 0.33962907826630584 1104 32-33 0.43559014130590157 1104 34-35 0.43606604127911197 1104 36-37 0.42650533466250096 1104 38-39 0.3392539408887174 1104 40-41 0.3512851054804287 1104 42-43 0.37517644877653566 1104 44-45 0.38478013918967946 1104 46-47 0.47178603907080685 1104 48-49 0.5613750886269813 1104 50-51 0.6360599005674956 1104 52-53 0.3751429335914338 1104 54-55 0.3901103615216428 1104 56-57 0.8892182410101697 1104 58-59 -7.999615597914307 1104 60-61 -7.114260809351631 1104 62-63 -1.8403653841618741 1104 64-65 -0.20996267904322252 1104 66-67 -1.473528639477795 1104 68-69 -2.9307341414996966 1104 70-71 -3.4918014858331237 1104 72-73 -2.6514153451317384 1104 74-75 -3.0637876825229213 1104 76-77 0.10966332739944917 1104 78-79 3.768709177580803 1104 80-81 3.208879233435063 1104 82-83 1.6264982616719905 1104 84-85 1.2166609604399206 1104 86-87 1.1892300598408703 1104 88-89 0.9763991938079357 1104 90-91 2.240975329917049 1104 92-93 1.8992087846202992 1104 94-95 -6.589635900917404 1104 96-97 -7.602296290484759 1104 98-99 -5.678836824705019 1104 100-101 -5.299811159528987 1105 1 0.1837236493636354 1105 2 0.14657252893309902 1105 3 0.216385383197089 1105 4 0.21315449220649896 1105 5 0.3835939682353171 1105 6 0.23671394592372508 1105 7 0.24496656920756266 1105 8 0.16849846880143815 1105 9 0.3272245437560528 1105 10-11 0.2665099954786214 1105 12-13 0.19444600463478423 1105 14-15 0.34165876341837986 1105 16-17 0.3768032844059377 1105 18-19 0.383216896397542 1105 20-21 0.3043356463222935 1105 22-23 0.3836002272891932 1105 24-25 0.45283468015814066 1105 26-27 0.2883148893336056 1105 28-29 0.49013805979608804 1105 30-31 0.4475751890949837 1105 32-33 0.3788203630414628 1105 34-35 0.43736037861437893 1105 36-37 0.3730134884304377 1105 38-39 0.37279691926265457 1105 40-41 0.3905666157850689 1105 42-43 0.23046798997554419 1105 44-45 0.4004508208212201 1105 46-47 0.3966388997332757 1105 48-49 0.6033433339048315 1105 50-51 0.7083423689887596 1105 52-53 0.6693598199079602 1105 54-55 0.5003048200879832 1105 56-57 1.067652013643226 1105 58-59 -7.791358783554291 1105 60-61 -6.855411526961422 1105 62-63 -1.5615517867272786 1105 64-65 0.05963168566679755 1105 66-67 -1.0348962042973184 1105 68-69 -2.9325467417108726 1105 70-71 -3.585196347199517 1105 72-73 -2.67615567597527 1105 74-75 -3.450860636259577 1105 76-77 0.037473835396792055 1105 78-79 3.885922329197868 1105 80-81 3.301708708230727 1105 82-83 1.8403604493064165 1105 84-85 1.469809351195071 1105 86-87 1.2157939407997667 1105 88-89 1.1367185986024317 1105 90-91 2.531847802640055 1105 92-93 2.1008078616998063 1105 94-95 -5.94822639405416 1105 96-97 -7.437284558444558 1105 98-99 -5.311230454207191 1105 100-101 -4.614816243413076 1106 1 0.06838904499677412 1106 2 0.08051947126713799 1106 3 0.1480233877383199 1106 4 0.054622823352246996 1106 5 0.1413266104840929 1106 6 0.19433641064296125 1106 7 0.1992264157254766 1106 8 0.09330335876762064 1106 9 0.027459078184136843 1106 10-11 0.12737340408396847 1106 12-13 0.09810294054978641 1106 14-15 0.14390916341071147 1106 16-17 0.24523157799552564 1106 18-19 0.08262385386902338 1106 20-21 0.14402197568603725 1106 22-23 0.046222644105156974 1106 24-25 0.1268943867984902 1106 26-27 0.14083776314680563 1106 28-29 0.16244632694306205 1106 30-31 0.1935323444069752 1106 32-33 0.17372412536457915 1106 34-35 0.22763103211083546 1106 36-37 0.25101659256257847 1106 38-39 0.1867671425877191 1106 40-41 0.13779435752893932 1106 42-43 0.06588747894343072 1106 44-45 0.20541427062338613 1106 46-47 0.043419799739979226 1106 48-49 -0.10571172611796698 1106 50-51 -0.15469278243349294 1106 52-53 -0.05596079670297627 1106 54-55 -0.15852940584301933 1106 56-57 0.3685539364806374 1106 58-59 -8.407062147322168 1106 60-61 -7.56446783246934 1106 62-63 -2.288364302894635 1106 64-65 -0.711156610605368 1106 66-67 0.37346971922989525 1106 68-69 -2.64354677045284 1106 70-71 -2.378612042228113 1106 72-73 -1.1064361017395683 1106 74-75 -1.3379922840363854 1106 76-77 1.7484613134810445 1106 78-79 3.7876047879770987 1106 80-81 2.795491725562133 1106 82-83 1.135106514052481 1106 84-85 0.771134451552431 1106 86-87 0.9139129708542804 1106 88-89 0.8348290060676788 1106 90-91 2.185037202603649 1106 92-93 1.6259491284539749 1106 94-95 -6.291305233262033 1106 96-97 -7.663365783235459 1106 98-99 -5.642316325615642 1106 100-101 -4.927572501975787 1107 1 -0.02853014690373712 1107 2 0.04686709223996388 1107 3 -0.010563196437107791 1107 4 -0.0579545260556813 1107 5 -0.00931021709992308 1107 6 0.018181167713386515 1107 7 0.02030344277099516 1107 8 -0.00759071142859824 1107 9 -0.09923822554931405 1107 10-11 -0.14042357159070917 1107 12-13 0.0028353242672096712 1107 14-15 -4.974511773809809E-4 1107 16-17 0.01796866972066624 1107 18-19 0.05528093757486374 1107 20-21 0.09007389619291217 1107 22-23 0.03110212717123062 1107 24-25 0.06066629699272852 1107 26-27 0.0988986543170256 1107 28-29 -0.05168442828011166 1107 30-31 -0.05068288715207103 1107 32-33 -0.06366323736322244 1107 34-35 -0.1357537028917264 1107 36-37 0.006823369681988822 1107 38-39 -0.06722918954580592 1107 40-41 -0.1657825335806251 1107 42-43 -0.29615842561140937 1107 44-45 -0.29303633690862085 1107 46-47 -0.24830088682722362 1107 48-49 -0.3572428088450792 1107 50-51 -0.3979326966329353 1107 52-53 -0.23112944051341344 1107 54-55 -0.2568125809666171 1107 56-57 0.13520128236348228 1107 58-59 -8.379131562324101 1107 60-61 -7.733147800022262 1107 62-63 -2.744059631025536 1107 64-65 -0.9266118628932531 1107 66-67 0.13342429997566896 1107 68-69 -2.832649312029819 1107 70-71 -2.5146191094182058 1107 72-73 -1.231806041731705 1107 74-75 -1.0445135849231306 1107 76-77 1.8691160328260068 1107 78-79 3.693565496382824 1107 80-81 2.4366937656001753 1107 82-83 1.0267948719054587 1107 84-85 0.5890658096284476 1107 86-87 0.39610286395128824 1107 88-89 0.34527212399091667 1107 90-91 1.6655514769886182 1107 92-93 1.0524250692694963 1107 94-95 -6.70078594311774 1107 96-97 -8.127182221239972 1107 98-99 -5.920320981838234 1107 100-101 -4.997974010550477 1108 1 -0.058449040409563224 1108 2 -0.027320222412043904 1108 3 -0.11985919321676519 1108 4 -0.21821297232219905 1108 5 -0.10819826830511658 1108 6 -0.05203224652115779 1108 7 -0.16271448439816538 1108 8 -0.1205806957919009 1108 9 -0.12969360369108074 1108 10-11 -0.20939667931082795 1108 12-13 -0.18312860019331367 1108 14-15 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62-63 0.41145196739587675 2313 64-65 -0.34944800744274573 2313 66-67 -1.886920606097842 2313 68-69 -4.690842260454154 2313 70-71 -3.6493950423683614 2313 72-73 -2.681221828727992 2313 74-75 -3.544285180206508 2313 76-77 0.2761319186071134 2313 78-79 2.7608123479684856 2313 80-81 2.1259785677244665 2313 82-83 0.6530012744845592 2313 84-85 0.3241200322221722 2313 86-87 0.43936801183652463 2313 88-89 0.48299691398522526 2313 90-91 2.0061006227883986 2313 92-93 3.3664499807747603 2313 94-95 5.379321580359484 2313 96-97 1.2401101529800034 2313 98-99 2.5989553384412893 2313 100-101 3.7126819797567894 2314 1 -0.3389341879731802 2314 2 -0.2878594595568025 2314 3 -0.3825681936498988 2314 4 -0.1769892457173512 2314 5 -0.31658984291785686 2314 6 -0.44858143850720467 2314 7 -0.4548857731626157 2314 8 -0.4235844629541745 2314 9 -0.4178224696585673 2314 10-11 -0.3934358664647206 2314 12-13 -0.47067506816568283 2314 14-15 -0.5951493047199463 2314 16-17 -0.48764562799094335 2314 18-19 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1.027120328118329 2314 84-85 0.6505942146740189 2314 86-87 0.6164915562351077 2314 88-89 0.7326447790103572 2314 90-91 2.4980774605188962 2314 92-93 3.992448189479454 2314 94-95 6.307986063232573 2314 96-97 3.371479811889264 2314 98-99 5.6119825057241925 2314 100-101 6.045684925581591 2315 1 -0.4638292559406487 2315 2 -0.4278889535809931 2315 3 -0.47046985744686864 2315 4 -0.31548528549564736 2315 5 -0.38521178689202884 2315 6 -0.5783398335594185 2315 7 -0.48966685845937974 2315 8 -0.6923594852936787 2315 9 -0.586722315494157 2315 10-11 -0.552387933067159 2315 12-13 -0.631021273102327 2315 14-15 -0.7185712738332981 2315 16-17 -0.7075298453408578 2315 18-19 -0.6831577556792681 2315 20-21 -0.7921916956611383 2315 22-23 -0.7277014366811159 2315 24-25 -0.80059802205691 2315 26-27 -0.7483648161963359 2315 28-29 -0.6840560871685284 2315 30-31 -0.6660838205419708 2315 32-33 -0.6459712560310251 2315 34-35 -0.6684223494763728 2315 36-37 -0.6185478649383 2315 38-39 -0.6278995332029993 2315 40-41 -0.5236527075305659 2315 42-43 -0.5908126470793817 2315 44-45 -0.5715683691529918 2315 46-47 -0.4124926560253499 2315 48-49 -0.32605910074155986 2315 50-51 -0.24943884852093134 2315 52-53 -0.2485244341493953 2315 54-55 -0.41591313251520745 2315 56-57 0.34733535109491953 2315 58-59 3.386460291727662 2315 60-61 3.4518331079115505 2315 62-63 0.8320964996490474 2315 64-65 0.09311660549877132 2315 66-67 -1.8960623355780015 2315 68-69 -4.837952663748107 2315 70-71 -3.3714286130244844 2315 72-73 -2.9812757065598774 2315 74-75 -0.7280095543758236 2315 76-77 1.9043302882354247 2315 78-79 3.782873939318762 2315 80-81 2.635039119864068 2315 82-83 0.9536086181931118 2315 84-85 0.7914417111982424 2315 86-87 0.9226196225427152 2315 88-89 1.1293196420223097 2315 90-91 2.625246003929643 2315 92-93 4.266468344619515 2315 94-95 6.742998528668426 2315 96-97 4.283933603961556 2315 98-99 6.600892425034907 2315 100-101 6.768466379776209 2316 1 -0.48249166074997873 2316 2 -0.4044686332945062 2316 3 -0.5508738829604845 2316 4 -0.41699916769292145 2316 5 -0.6405967562943928 2316 6 -0.6351905055569986 2316 7 -0.6181438796655954 2316 8 -0.6764456548715003 2316 9 -0.7716886616825747 2316 10-11 -0.6109709134429622 2316 12-13 -0.594078394694705 2316 14-15 -0.7895565466952377 2316 16-17 -0.7422682372927056 2316 18-19 -0.7300444185586841 2316 20-21 -0.6204538861791136 2316 22-23 -0.8425093834390651 2316 24-25 -0.8115073760906952 2316 26-27 -0.8737851292918393 2316 28-29 -0.7304821674343103 2316 30-31 -0.7333751741011483 2316 32-33 -0.7759123618607546 2316 34-35 -0.7098881779138466 2316 36-37 -0.7327888024989662 2316 38-39 -0.9179690520541612 2316 40-41 -1.100324611631585 2316 42-43 -1.1296134963347484 2316 44-45 -1.09583992233393 2316 46-47 -1.2127320259512118 2316 48-49 -1.2498378916492783 2316 50-51 -1.2659712834604946 2316 52-53 -1.164499837383893 2316 54-55 -0.9851007489192725 2316 56-57 -0.2983300128048967 2316 58-59 2.8160676459484115 2316 60-61 2.7622425985740584 2316 62-63 0.13203267965379695 2316 64-65 -0.5016977052812344 2316 66-67 -2.423237608655672 2316 68-69 -3.3539427186153254 2316 70-71 -3.203854384077893 2316 72-73 -0.8009968940792014 2316 74-75 5.093969872214998 2316 76-77 5.339490970803755 2316 78-79 4.298423726106044 2316 80-81 2.689002431456654 2316 82-83 1.0411417866131778 2316 84-85 0.5967803886322116 2316 86-87 0.5833838085591267 2316 88-89 0.5590323441788883 2316 90-91 1.9100793168885168 2316 92-93 3.4334750250449346 2316 94-95 5.911888618052377 2316 96-97 3.508108438389117 2316 98-99 5.750457010870107 2316 100-101 5.926400463608717 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 13.0 4 45.0 5 133.0 6 299.0 7 712.0 8 1497.0 9 2640.0 10 4419.0 11 6645.0 12 9703.0 13 13006.0 14 16008.0 15 20940.0 16 27608.0 17 36075.0 18 36737.0 19 33152.0 20 33525.0 21 34130.0 22 34355.0 23 35019.0 24 36739.0 25 39044.0 26 48332.0 27 66839.0 28 94026.0 29 123701.0 30 149368.0 31 165014.0 32 171458.0 33 172192.0 34 176905.0 35 188137.0 36 182968.0 37 133686.0 38 48891.0 39 2792.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.896518160295926 19.664074945729485 14.546688019204238 21.89271887477035 2 15.464867524122946 22.241048700584763 38.82079884314215 23.473284932150136 3 19.382583549473807 27.107649136928718 30.715983960886028 22.793783352711444 4 12.399732433495004 17.331918485379802 37.97264337446827 32.29570570665693 5 12.667507525311988 39.26730635314385 33.7581906810116 14.306995440532555 6 29.673935929374366 37.5576451166318 17.750177477086357 15.018241476907482 7 26.79857422082435 34.56587520891988 21.324314453994774 17.311236116261 8 26.14124680389407 33.68095910428447 20.248347154982433 19.92944693683903 9 26.981687718585622 16.573378623374058 20.71302001717941 35.73191364086091 10-11 24.61569922245472 26.633231722655022 27.530422647007857 21.220646407882406 12-13 26.814581353116367 24.549255842130137 27.710980593737855 20.925182211015645 14-15 23.31316950103133 25.28245047858435 27.629046352902158 23.775333667482162 16-17 21.2049715943498 28.819553782544453 27.853235300145897 22.122239322959853 18-19 21.032530164257903 27.585191727386473 30.587712752230583 20.794565356125037 20-21 22.56793506108752 26.10648345689962 29.940663959947006 21.38491752206585 22-23 21.932581066502202 26.249559800927543 29.87770850529823 21.940150627272033 24-25 22.018162296116696 26.409930336717512 30.22324391931077 21.348663447855017 26-27 22.121364571178358 25.998518694494717 30.704551753693366 21.17556498063356 28-29 21.335070546733107 26.636335305787913 30.715394042948052 21.31320010453093 30-31 21.456106474612355 26.664515993785564 30.429513443258337 21.44986408834374 32-33 21.413907124342916 26.76480425899045 30.15437815497165 21.66691046169499 34-35 22.031038193328396 26.275673006600798 30.07956361129356 21.613725188777245 36-37 22.25823258112529 26.058373146627535 30.33683270723796 21.346561565009214 38-39 22.081285599336894 26.245399882706916 30.37576210202634 21.297552415929854 40-41 21.692645734223742 26.450302387208545 30.4611987699377 21.395853108630018 42-43 21.43623924204105 26.980852645538846 29.565420302578417 22.01748780984169 44-45 21.708106903432054 26.773065310888615 29.266543127305145 22.25228465837419 46-47 22.007106195239007 27.040210472430044 28.6564712672777 22.29621206505325 48-49 22.12566838687421 26.919896134894106 28.683365266725406 22.271070211506277 50-51 21.91558664797392 27.28541511525055 28.89786233796806 21.901135898807464 52-53 22.045757350547284 27.580746312170834 28.33055888233321 22.04293745494867 54-55 21.367447676300404 27.12751120785215 28.671968264051145 22.833072851796295 56-57 21.922745156626664 27.56138893984062 28.090458269598344 22.425407633934366 58-59 21.240926455086274 26.72028500536449 29.70873176084357 22.33005677870566 60-61 21.385160992539014 26.943963729207688 29.631063877025188 22.039811401228114 62-63 22.396558410477564 26.903233244785852 28.495178024696244 22.205030320040343 64-65 21.945094988488183 27.391505363446118 28.54128939317536 22.122110254890337 66-67 21.650798285343598 26.87800438671365 28.89763510689524 22.573562221047514 68-69 20.99614466083461 26.74836507004526 30.097783382691663 22.15770688642847 70-71 21.924502434924626 26.7984764584215 28.87523768083115 22.401783425822718 72-73 21.297328648465616 26.66353035355133 29.247645275398803 22.79149572258425 74-75 21.178221159255713 27.438233156550545 28.876611148709134 22.506934535484607 76-77 20.296627697092685 27.242263862659293 29.366737970499123 23.0943704697489 78-79 21.36047650054105 27.10811203290346 28.682971617019483 22.848439849536007 80-81 21.387089025049182 27.831532278602378 28.024223659938624 22.757155036409817 82-83 21.45886470790867 27.521326991037387 27.86102629950416 23.15878200154978 84-85 21.365337365513447 27.243534874794427 27.672000594392536 23.719127165299586 86-87 21.46778383711435 27.298178208468403 27.82791339211723 23.406124562300015 88-89 21.60106262683384 26.907256089480146 27.7835552302067 23.708126053479308 90-91 21.25487998627867 26.869151289564197 28.25301456792887 23.62295415622826 92-93 21.003510943676222 27.430872907381094 28.15422145185188 23.411394697090806 94-95 20.08278326701189 27.185239609477403 28.65460145417355 24.077375669337158 96-97 19.620377250196412 27.0838572130122 28.58228332454873 24.71348221224266 98-99 20.482605999269673 26.859531250512703 28.04722558035863 24.610637169858993 100-101 19.74127360562143 26.890797833406527 28.731512223686135 24.6364163372859 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2421.0 1 1795.0 2 1192.0 3 1244.0 4 1271.5 5 1417.0 6 1893.5 7 2739.5 8 3722.0 9 4381.0 10 4396.5 11 4310.5 12 4455.5 13 4531.0 14 4586.0 15 4736.0 16 4921.5 17 5125.0 18 5363.0 19 5668.5 20 6116.0 21 6628.5 22 7292.5 23 8271.5 24 9359.0 25 10819.5 26 13006.0 27 15566.5 28 18158.0 29 21078.5 30 24404.0 31 28357.5 32 32908.0 33 37442.5 34 41814.0 35 46019.0 36 50159.0 37 54161.5 38 56937.5 39 58426.5 40 60073.5 41 62289.0 42 64710.0 43 67646.5 44 71493.0 45 75978.5 46 81889.5 47 90283.5 48 100305.5 49 97788.0 50 85368.5 51 77422.0 52 72050.0 53 68178.0 54 64392.5 55 59894.5 56 55080.0 57 49943.0 58 44900.0 59 39680.0 60 33749.5 61 27719.5 62 22128.5 63 16919.0 64 12292.0 65 8861.0 66 6248.0 67 4345.0 68 2949.5 69 1854.5 70 1252.0 71 898.0 72 670.0 73 529.0 74 377.0 75 256.0 76 182.5 77 108.0 78 56.0 79 39.0 80 25.0 81 13.0 82 9.0 83 7.0 84 5.0 85 3.0 86 3.5 87 3.0 88 1.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02622561669793866 2 0.011738641932292258 3 1.8632764971892476E-4 4 0.0 5 0.061953943531542474 6 0.0 7 0.0 8 1.3974573728919355E-4 9 0.0 10-11 0.0 12-13 2.3290956214865595E-5 14-15 3.726552994378495E-4 16-17 0.0 18-19 4.658191242973119E-4 20-21 0.0 22-23 0.0 24-25 4.658191242973119E-5 26-27 4.658191242973119E-5 28-29 0.0010713839858838173 30-31 0.006568049652592097 32-33 0.00670779538988129 34-35 0.0021427679717676345 36-37 0.0861066651263581 38-39 0.07928241495540247 40-41 0.08496540827182968 42-43 0.08806310544840681 44-45 0.09132383931848799 46-47 0.08696843050630812 48-49 0.07760546610793216 50-51 0.07166627227314143 52-53 0.05992763034084917 54-55 0.048934299007432604 56-57 0.06891793943978729 58-59 0.7055762275731382 60-61 0.3397451783062444 62-63 0.003423770563585242 64-65 0.0022592227528419623 66-67 1.085288686744092 68-69 2.3376201114612 70-71 2.3136537175161034 72-73 1.775842247558642 74-75 1.262905518838657 76-77 0.7329431011256053 78-79 0.5601940788799472 80-81 0.03901235165989987 82-83 2.0961860593379032E-4 84-85 0.0012344206793878764 86-87 0.0010713839858838173 88-89 2.3290956214865595E-5 90-91 0.3270748981253575 92-93 0.05492007475465307 94-95 0.9371582052175469 96-97 1.6353511996705725 98-99 0.627132287041471 100-101 0.5621738101582109 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2146756.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.720776746805825 #Duplication Level Percentage of deduplicated Percentage of total 1 67.33177316495372 26.74470329851698 2 14.877864197568744 11.81920644521847 3 6.324541734738094 7.536471308141637 4 3.3871592200049623 5.381623807348081 5 2.0425512943512043 4.056586197841173 6 1.2973897633374047 3.091999748586978 7 0.9007489813341423 2.5044914434740364 8 0.6699570590197063 2.1288971817014692 9 0.4773779273720925 1.7065639869299796 >10 2.431580438277941 17.098093189293216 >50 0.16710596295885596 4.556299398041901 >100 0.08290975440948051 5.922521407566739 >500 0.004109318942546038 1.1217778101528857 >1k 0.004109318942546037 3.339030832764874 >5k 5.870455632208623E-4 1.73242712611455 >10k+ 2.3481822528834492E-4 1.2593068183070744 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15536 0.7236965915083037 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11467 0.5341547898317275 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 9281 0.43232672926033516 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9109 0.42431464032242133 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 7402 0.3447993158048702 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 6317 0.2942579408186119 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5039 0.23472625673341546 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4031 0.1877716890042464 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 4013 0.18693321458051124 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3833 0.17854847034315963 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3454 0.1608939255322915 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 3145 0.14650011459150458 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3111 0.14491632956889372 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 3093 0.14407785514515856 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2663 0.12404763280037415 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2537 0.11817831183422803 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2511 0.11696718211105502 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2468 0.11496415987657656 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2328 0.10844269213641419 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2273 0.10588068695277898 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2256 0.10508879444147355 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 4.658191242973118E-5 0.0 3 0.0 0.0 0.0 4.658191242973118E-5 0.0 4 0.0 0.0 0.0 4.658191242973118E-5 0.0 5 0.0 0.0 0.0 4.658191242973118E-5 0.0 6 0.0 0.0 0.0 4.658191242973118E-5 0.0 7 0.0 0.0 0.0 4.658191242973118E-5 0.0 8 0.0 0.0 0.0 4.658191242973118E-5 0.0 9 0.0 0.0 0.0 4.658191242973118E-5 0.0 10-11 0.0 0.0 0.0 1.1645478107432796E-4 0.0 12-13 0.0 0.0 0.0 1.8632764971892473E-4 0.0 14-15 4.658191242973118E-5 0.0 0.0 1.8632764971892473E-4 6.987286864459677E-5 16-17 4.658191242973118E-5 0.0 0.0 3.260733870081183E-4 9.316382485946237E-5 18-19 9.316382485946237E-5 0.0 0.0 4.6581912429731183E-4 9.316382485946237E-5 20-21 9.316382485946237E-5 0.0 0.0 9.549292048094892E-4 9.316382485946237E-5 22-23 1.1645478107432796E-4 0.0 0.0 0.0016536578912554572 1.1645478107432796E-4 24-25 1.3974573728919355E-4 0.0 0.0 0.00295795143928793 1.3974573728919355E-4 26-27 1.3974573728919355E-4 0.0 0.0 0.004448572637039329 1.3974573728919355E-4 28-29 2.5620051836352153E-4 0.0 0.0 0.008571071887070538 1.6303669350405914E-4 30-31 2.794914745783871E-4 0.0 0.0 0.01933149365833844 1.8632764971892473E-4 32-33 2.794914745783871E-4 0.0 0.0 0.03989740799606476 2.794914745783871E-4 34-35 2.794914745783871E-4 0.0 0.0 0.07217867330986846 2.794914745783871E-4 36-37 3.260733870081183E-4 0.0 0.0 0.1212294270983754 2.794914745783871E-4 38-39 4.1923721186758065E-4 0.0 0.0 0.19443290248169798 3.7265529943784946E-4 40-41 4.1923721186758065E-4 0.0 0.0 0.2765568140953141 3.7265529943784946E-4 42-43 4.6581912429731183E-4 0.0 0.0 0.35805186989112875 3.9594625565271503E-4 44-45 5.589829491567742E-4 0.0 0.0 0.4523336606489047 4.1923721186758065E-4 46-47 5.589829491567742E-4 0.0 0.0 0.5624067197203595 4.1923721186758065E-4 48-49 5.589829491567742E-4 0.0 0.0 0.6732716713031197 4.1923721186758065E-4 50-51 5.589829491567742E-4 0.0 0.0 0.7940119883209829 4.1923721186758065E-4 52-53 5.822739053716398E-4 0.0 0.0 0.9077883094306014 4.1923721186758065E-4 54-55 6.055648615865054E-4 0.0 0.0 1.0280395163679523 4.1923721186758065E-4 56-57 6.055648615865054E-4 0.0 0.0 1.1399525609803816 4.1923721186758065E-4 58-59 6.055648615865054E-4 0.0 0.0 1.2288075589400937 4.1923721186758065E-4 60-61 6.055648615865054E-4 0.0 2.329095621486559E-5 1.313167402350337 4.1923721186758065E-4 62-63 6.055648615865054E-4 0.0 4.658191242973118E-5 1.3844610193240405 4.1923721186758065E-4 64-65 6.055648615865054E-4 2.329095621486559E-5 4.658191242973118E-5 1.4560807096847523 4.1923721186758065E-4 66-67 6.055648615865054E-4 4.658191242973118E-5 4.658191242973118E-5 1.5201075483194177 4.1923721186758065E-4 68-69 6.055648615865054E-4 4.658191242973118E-5 4.658191242973118E-5 1.597317068171697 4.1923721186758065E-4 70-71 6.055648615865054E-4 4.658191242973118E-5 4.658191242973118E-5 1.7175216931966184 4.1923721186758065E-4 72-73 6.055648615865054E-4 4.658191242973118E-5 4.658191242973118E-5 1.8816297706865615 4.1923721186758065E-4 74-75 6.055648615865054E-4 4.658191242973118E-5 4.658191242973118E-5 2.0948118929212263 4.1923721186758065E-4 76-77 6.055648615865054E-4 4.658191242973118E-5 4.658191242973118E-5 2.3641485105899323 4.1923721186758065E-4 78-79 6.055648615865054E-4 4.658191242973118E-5 4.658191242973118E-5 2.7111604672352145 4.1923721186758065E-4 80-81 6.521467740162366E-4 4.658191242973118E-5 4.658191242973118E-5 3.1324938651621332 4.4252816808244627E-4 82-83 6.521467740162366E-4 4.658191242973118E-5 4.658191242973118E-5 3.538338777206166 4.6581912429731183E-4 84-85 6.754377302311022E-4 6.987286864459677E-5 4.658191242973118E-5 3.91714288908474 4.6581912429731183E-4 86-87 6.987286864459678E-4 9.316382485946237E-5 4.658191242973118E-5 4.259030835362752 4.6581912429731183E-4 88-89 6.987286864459678E-4 9.316382485946237E-5 4.658191242973118E-5 4.587130535561563 4.6581912429731183E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 13735 0.0 24.774357 1 TACATGG 13900 0.0 23.862995 2 ACATGGG 13620 0.0 23.41441 3 GTCGGGA 585 0.0 22.58326 2 AGTACTT 11140 0.0 22.030098 12-13 GAGTACT 10885 0.0 21.809814 12-13 CATGGGA 10000 0.0 21.747759 4 ATGGGAG 3420 0.0 21.104689 5 GTACTTT 11975 0.0 19.804934 14-15 CGGGAGT 745 0.0 18.996717 4 CATGGGG 3985 0.0 18.822721 4 TACTTTT 13110 0.0 18.683737 14-15 ACTTTTT 13595 0.0 17.913153 16-17 GTATCAA 32370 0.0 17.67078 1 TCGGGAG 830 0.0 17.619646 3 ATGGGAT 3720 0.0 17.500473 5 AGTACAT 10400 0.0 17.103794 2 CGCATAG 595 0.0 16.742504 66-67 ACGCATA 700 0.0 16.660234 64-65 GGTCGGG 895 0.0 15.819546 1 >>END_MODULE