FastQCFastQC Report
Fri 27 May 2016
SRR939597_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939597_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3305028
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68550.20741125339936603No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49920.15104259328514008No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33340.10087660376856111No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA117300.028.6857411
GGTATCA79550.026.1904051
ATCAACG133300.024.8161073
TCAACGC133500.024.7781774
CAACGCA135350.024.5088885
TATCAAC138300.024.0565552
AACGCAG139500.023.8101846
GTACATG87000.022.8021031
TACATGG88050.021.9921862
ACGCAGA155450.021.0008417
ACATGGG88100.020.901953
GTATAGG10450.020.891011
CGCAGAG155950.020.5388958
AGAGTAC104550.020.06150410-11
GAGTACT68450.018.89114612-13
AGTACTT72600.018.36686112-13
GCAGAGT162300.018.2152359
GTACTTT73800.017.49044414-15
CATGGGG57750.017.257794
TATACCG2755.9703416E-717.2572675
Produced by FastQC (version 0.11.5)