##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939597_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3305028 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.982929342807385 31.0 30.0 33.0 26.0 34.0 2 30.1358345526876 31.0 30.0 34.0 25.0 34.0 3 30.17632437607185 31.0 30.0 34.0 26.0 34.0 4 33.74696795307029 35.0 35.0 37.0 28.0 37.0 5 33.726221381483 35.0 33.0 37.0 28.0 37.0 6 33.813625482144175 35.0 35.0 37.0 28.0 37.0 7 33.60491620645877 35.0 33.0 37.0 28.0 37.0 8 33.594173483552936 35.0 33.0 37.0 28.0 37.0 9 34.85676853569773 37.0 34.0 39.0 27.0 39.0 10-11 34.73314931068663 37.0 34.0 39.0 27.0 39.0 12-13 34.577542913403455 37.0 34.0 39.0 27.0 39.0 14-15 35.26660848259077 38.0 33.5 40.0 27.0 41.0 16-17 34.964695155381435 38.0 33.0 40.0 25.5 41.0 18-19 34.88921939541814 38.0 33.0 40.0 25.0 41.0 20-21 35.00201117812013 38.0 34.0 40.0 25.0 41.0 22-23 34.84458981890622 38.0 33.0 40.0 25.0 41.0 24-25 34.721385870255865 38.0 33.0 40.0 25.0 41.0 26-27 34.85737185887684 38.0 34.0 40.0 25.0 41.0 28-29 34.730216052632535 38.0 33.0 40.0 24.0 41.0 30-31 34.518261267378065 38.0 33.0 40.0 24.0 41.0 32-33 34.29605029064807 38.0 33.0 40.0 24.0 41.0 34-35 34.07689707923806 38.0 33.0 40.0 23.0 41.0 36-37 33.79995494743161 38.0 32.5 40.0 21.0 41.0 38-39 33.63882484505426 38.0 32.0 40.0 20.0 41.0 40-41 33.362188156953586 38.0 31.5 40.0 18.5 41.0 42-43 33.05562207037278 37.0 31.0 40.0 17.0 41.0 44-45 32.81267087601073 37.0 31.0 40.0 15.5 41.0 46-47 32.39248547969942 37.0 30.5 40.0 14.0 41.0 48-49 32.05569786398179 37.0 30.0 39.5 10.5 41.0 50-51 31.451978319094422 35.5 29.5 38.5 9.0 40.0 52-53 31.500147048678556 36.0 30.0 39.0 8.5 40.0 54-55 31.52517663995585 36.0 30.0 39.0 8.0 40.0 56-57 31.359696347504467 36.0 29.5 39.0 7.0 40.0 58-59 30.935708714116792 35.5 28.5 39.0 4.0 40.0 60-61 30.490517629502683 35.0 28.0 39.0 2.0 40.0 62-63 30.052364306747172 35.0 28.0 38.0 2.0 40.0 64-65 29.407943291251996 34.0 26.5 38.0 2.0 40.0 66-67 28.843613427783367 34.0 26.0 37.5 2.0 39.5 68-69 28.30590013155713 34.0 26.0 37.0 2.0 39.0 70-71 27.733220565756177 33.0 25.0 36.0 2.0 39.0 72-73 26.965428280789148 32.0 23.0 36.0 2.0 38.5 74-75 26.486671519878197 32.0 22.0 35.0 2.0 38.0 76-77 26.087145403911858 32.0 21.5 35.0 2.0 37.0 78-79 25.493992940453154 31.0 20.0 35.0 2.0 36.5 80-81 24.80760510954824 31.0 18.0 34.0 2.0 36.0 82-83 24.128507988434592 30.5 12.0 34.0 2.0 35.5 84-85 23.446638424848445 30.0 7.0 34.0 2.0 35.0 86-87 22.756261520326 29.5 2.0 34.0 2.0 35.0 88-89 21.9353944959014 29.0 2.0 34.0 2.0 35.0 90-91 20.675594881495712 27.5 2.0 33.5 2.0 34.5 92-93 19.02395743697179 25.0 2.0 32.0 2.0 34.0 94-95 18.72011734847632 25.0 2.0 32.0 2.0 34.0 96-97 18.060572104078997 24.5 2.0 32.0 2.0 34.0 98-99 17.05565384014901 21.0 2.0 32.0 2.0 34.0 100-101 15.462590937202348 2.0 2.0 30.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.0285806830119455 1101 2 1.1146227191185822 1101 3 0.9766403809806725 1101 4 1.1719284855742913 1101 5 0.9350850149754706 1101 6 0.8939625913148035 1101 7 1.00032167393789 1101 8 0.9887214875733719 1101 9 1.2376406264043354 1101 10-11 0.9975756077492122 1101 12-13 0.9376064301912308 1101 14-15 1.0791988433555346 1101 16-17 0.9187145474890102 1101 18-19 1.0357232456647978 1101 20-21 1.243773756759417 1101 22-23 1.367918488522612 1101 24-25 1.2820433052979112 1101 26-27 1.2471680029656014 1101 28-29 1.357197011274586 1101 30-31 1.3398065739672163 1101 32-33 1.1245927384004801 1101 34-35 1.3381434437393764 1101 36-37 1.3511769454748475 1101 38-39 1.1464356399240572 1101 40-41 1.1968123585246744 1101 42-43 1.3207534873788305 1101 44-45 1.2659306077337575 1101 46-47 1.0930726727132125 1101 48-49 1.0167779281627602 1101 50-51 1.0057332596569104 1101 52-53 1.162867101796298 1101 54-55 1.4314332656029443 1101 56-57 1.2688908646218273 1101 58-59 1.146469464124035 1101 60-61 1.0714380305708708 1101 62-63 1.138219900885133 1101 64-65 0.9493182042612638 1101 66-67 0.9003547540159502 1101 68-69 0.8349399183835118 1101 70-71 0.8892253376922419 1101 72-73 0.9365290031890261 1101 74-75 0.6879866105661101 1101 76-77 0.06754008739885009 1101 78-79 0.27652840983936144 1101 80-81 0.22669895699692333 1101 82-83 0.2831867968112647 1101 84-85 -0.03878784543327285 1101 86-87 -0.6446058387059743 1101 88-89 -0.9502139198373563 1101 90-91 -4.414790869334055 1101 92-93 -9.548600414878528 1101 94-95 -7.8219652800501045 1101 96-97 -4.60672861786955 1101 98-99 -3.495754413746294 1101 100-101 -2.8456197177544844 1102 1 1.104702314642676 1102 2 1.0306558383565765 1102 3 1.1161750565817528 1102 4 1.100960908357571 1102 5 1.129016593958852 1102 6 0.9892248831087969 1102 7 1.1212189476572263 1102 8 1.013249801379125 1102 9 1.3739118878822865 1102 10-11 1.0554928186033834 1102 12-13 1.0905477274289481 1102 14-15 1.1829713195494733 1102 16-17 1.4661912546848157 1102 18-19 1.3746160432281016 1102 20-21 1.318148658384537 1102 22-23 1.3403102182413278 1102 24-25 1.3971960120746871 1102 26-27 1.2540885281650986 1102 28-29 1.2968608968601885 1102 30-31 1.3269251228777605 1102 32-33 1.4707228348776198 1102 34-35 1.284252562347774 1102 36-37 1.3610682606371824 1102 38-39 1.4320266209671217 1102 40-41 1.23230937345555 1102 42-43 1.133725588129444 1102 44-45 0.945035610784366 1102 46-47 1.0223315880442243 1102 48-49 1.1228657346252149 1102 50-51 1.0114310409511837 1102 52-53 1.0512096975035483 1102 54-55 1.1591861963191228 1102 56-57 1.03895591970387 1102 58-59 1.0971817377828295 1102 60-61 1.0548559070841357 1102 62-63 0.749629887403497 1102 64-65 0.7884737516046556 1102 66-67 0.8559249703906922 1102 68-69 0.7757629825631582 1102 70-71 0.8357501971247281 1102 72-73 0.5646534422218679 1102 74-75 0.4902430869895653 1102 76-77 0.4088233617993744 1102 78-79 0.1838307743636527 1102 80-81 0.04210674097067724 1102 82-83 -0.07748159440992808 1102 84-85 -0.2783679379885804 1102 86-87 -0.6808124030680283 1102 88-89 -1.4169939380620455 1102 90-91 -5.339270936673806 1102 92-93 -10.254761012176623 1102 94-95 -8.939391400839462 1102 96-97 -5.676584328561312 1102 98-99 -4.362471445722672 1102 100-101 -3.7005681522053564 1103 1 0.8992019028781826 1103 2 0.9322667989106286 1103 3 0.9430707150965709 1103 4 0.9812861841672245 1103 5 0.8587811807613477 1103 6 0.7603246389524045 1103 7 0.94232813599141 1103 8 0.7838062820443383 1103 9 0.8622019950136064 1103 10-11 0.7673129990541838 1103 12-13 0.7611198079824177 1103 14-15 0.8756204163884931 1103 16-17 0.8912582797861219 1103 18-19 0.9210125597367664 1103 20-21 0.9627582621110875 1103 22-23 0.9557176474160158 1103 24-25 0.8017428353206952 1103 26-27 0.8078931776573768 1103 28-29 0.9512118145326838 1103 30-31 1.0150021826978914 1103 32-33 1.1920471565545654 1103 34-35 1.0298555705866974 1103 36-37 1.127036378819028 1103 38-39 1.006298868040325 1103 40-41 0.9326423222334981 1103 42-43 0.8419745732297415 1103 44-45 0.8770078538668997 1103 46-47 0.8294815972953202 1103 48-49 0.7168137942530635 1103 50-51 0.5739108608631689 1103 52-53 0.843211489676758 1103 54-55 0.9642374764169226 1103 56-57 0.6002825269458505 1103 58-59 0.6481625354087477 1103 60-61 0.35497326903419335 1103 62-63 0.4830268900482224 1103 64-65 0.4440028236289777 1103 66-67 0.44559439787478894 1103 68-69 0.42683792370078777 1103 70-71 0.6485950725614344 1103 72-73 0.6434433080991084 1103 74-75 0.2810367947927759 1103 76-77 0.0936852120912377 1103 78-79 0.03164524815765546 1103 80-81 -0.15837449744277876 1103 82-83 -0.07378387670986442 1103 84-85 -0.41046770108794917 1103 86-87 -0.6082103417060445 1103 88-89 -0.978015349815653 1103 90-91 -2.5806293241532643 1103 92-93 -9.212180572985348 1103 94-95 -5.8832340705245265 1103 96-97 -3.240804624378729 1103 98-99 -2.4756409258110477 1103 100-101 -2.032492053773689 1104 1 0.8434277513206929 1104 2 0.8715811688317494 1104 3 0.9029988596376874 1104 4 0.9089526923110824 1104 5 0.8729286154248186 1104 6 0.6753939968628515 1104 7 0.9153137718531639 1104 8 0.9431412467279046 1104 9 1.1400232983492486 1104 10-11 0.801519994636763 1104 12-13 0.7975898685159493 1104 14-15 0.997725757744611 1104 16-17 1.1064413322629818 1104 18-19 0.9722416132270837 1104 20-21 1.0063938441358218 1104 22-23 1.0009577777867307 1104 24-25 0.9110321223738111 1104 26-27 0.9396705229081732 1104 28-29 0.8256163747788818 1104 30-31 0.9166636443415825 1104 32-33 0.948358604498047 1104 34-35 1.0119202241280973 1104 36-37 0.751536594070302 1104 38-39 0.6853717270075705 1104 40-41 0.5297176071894683 1104 42-43 0.5140966778669451 1104 44-45 0.5367260106963982 1104 46-47 0.42208699670018746 1104 48-49 0.456227291541758 1104 50-51 0.6004528692758804 1104 52-53 0.5785992909548163 1104 54-55 0.6433347167381207 1104 56-57 0.7225632908233486 1104 58-59 0.6362466987863478 1104 60-61 0.4259134589323743 1104 62-63 0.39646168502171975 1104 64-65 0.4436899589263916 1104 66-67 0.38428189413946967 1104 68-69 0.3355041395324534 1104 70-71 0.4218903340144884 1104 72-73 0.32833464075662633 1104 74-75 0.042302146573906185 1104 76-77 0.02373193891207137 1104 78-79 -0.15948964753636474 1104 80-81 0.10694946957778484 1104 82-83 0.01999248782957963 1104 84-85 0.012196492587666086 1104 86-87 -0.05143813898450489 1104 88-89 -0.2859492976471252 1104 90-91 -0.8181772077681231 1104 92-93 -4.392883021797488 1104 94-95 -1.9194449581457604 1104 96-97 -1.1627976706757508 1104 98-99 -0.9975775429166713 1104 100-101 -0.6110141846054429 1105 1 0.9168428164194893 1105 2 0.8731172102687914 1105 3 0.7839456369842601 1105 4 0.6239023938152002 1105 5 0.6765204564712022 1105 6 0.6387948191861454 1105 7 0.7871599481516753 1105 8 0.7337135874356662 1105 9 1.0406671877946607 1105 10-11 0.7347486669154364 1105 12-13 0.8763410335142297 1105 14-15 0.8450437308361884 1105 16-17 0.8938721584000646 1105 18-19 0.8289659198529904 1105 20-21 0.619245724535169 1105 22-23 0.641007249057246 1105 24-25 0.8856813973714068 1105 26-27 0.7199613195965497 1105 28-29 0.6873715666429447 1105 30-31 0.771850528663883 1105 32-33 0.8867874476244424 1105 34-35 0.8327385049371472 1105 36-37 0.7396534606044582 1105 38-39 0.7179024834367809 1105 40-41 0.620307631619589 1105 42-43 0.8291365499572763 1105 44-45 0.7393022848025552 1105 46-47 0.7473174482254237 1105 48-49 0.5314675170131054 1105 50-51 0.5457378874692331 1105 52-53 0.5188633057362999 1105 54-55 0.7167726092872009 1105 56-57 0.675438371506953 1105 58-59 0.4795016877924958 1105 60-61 0.4405602242049973 1105 62-63 0.5093386367795034 1105 64-65 0.6432539589412372 1105 66-67 0.657098664280511 1105 68-69 0.49756160308599107 1105 70-71 0.40011631230882116 1105 72-73 0.41323035987180035 1105 74-75 0.35545385625499293 1105 76-77 0.11069002538314265 1105 78-79 -0.02451826987945438 1105 80-81 -0.08149691486909916 1105 82-83 -0.14973784705558657 1105 84-85 -0.14372232868392487 1105 86-87 -0.4031428230651066 1105 88-89 -0.677014978823852 1105 90-91 -1.8444172971378059 1105 92-93 -7.4219357637367445 1105 94-95 -3.6227200801863155 1105 96-97 -1.8392549401484963 1105 98-99 -1.4549824700384182 1105 100-101 -1.2308481146888894 1106 1 0.8229607517200499 1106 2 0.8333304720171277 1106 3 0.819101144201543 1106 4 0.836795840060482 1106 5 0.7180402086909368 1106 6 0.7293544153221561 1106 7 0.7499721165427573 1106 8 0.670993452038104 1106 9 0.8109374120875117 1106 10-11 0.8175215138367307 1106 12-13 0.867672230563123 1106 14-15 0.9034715449495749 1106 16-17 0.9706602617416351 1106 18-19 0.8196788381551414 1106 20-21 0.786037285020825 1106 22-23 0.8182096520842563 1106 24-25 0.639940356931703 1106 26-27 0.766687579941987 1106 28-29 0.7550399639608543 1106 30-31 0.8594579430187608 1106 32-33 0.7891045563859294 1106 34-35 0.7394435782251989 1106 36-37 0.8106829807210971 1106 38-39 0.556939631952595 1106 40-41 0.672973223906439 1106 42-43 0.7918786870809527 1106 44-45 0.7486420553419393 1106 46-47 0.6124867027411725 1106 48-49 0.8597359641562221 1106 50-51 0.6287119443342384 1106 52-53 0.8016988500162903 1106 54-55 0.8629018270880344 1106 56-57 0.7333922223193703 1106 58-59 0.6586075520085792 1106 60-61 0.7013121974611813 1106 62-63 0.7322522248111696 1106 64-65 0.7815647669678576 1106 66-67 0.6820245029640049 1106 68-69 0.4651260687105605 1106 70-71 0.6938518311469473 1106 72-73 0.9502787432552786 1106 74-75 0.8918118031688387 1106 76-77 0.6650715727395919 1106 78-79 0.6859806457227542 1106 80-81 0.6574685295676588 1106 82-83 0.5836141329296431 1106 84-85 0.38837500475979425 1106 86-87 0.554299619927928 1106 88-89 0.651341503576468 1106 90-91 0.2868076414029055 1106 92-93 -3.6242162124056208 1106 94-95 -1.0643324797089306 1106 96-97 -0.02468993922419571 1106 98-99 0.14154504705166815 1106 100-101 0.2958113050174749 1107 1 0.5620905792677924 1107 2 0.6408966062108163 1107 3 0.4595624666417102 1107 4 0.5151468860408741 1107 5 0.40172538911997435 1107 6 0.5747156014727324 1107 7 0.40033364951002426 1107 8 0.4492820710140393 1107 9 0.39632236924158804 1107 10-11 0.26957677767049404 1107 12-13 0.3558396417921088 1107 14-15 0.5634151544302313 1107 16-17 0.5152620130721957 1107 18-19 0.2420577475759984 1107 20-21 0.3583133679793846 1107 22-23 0.4836387299455396 1107 24-25 0.4456098724944013 1107 26-27 0.2630971835773863 1107 28-29 0.4276884530586287 1107 30-31 0.43171926283437045 1107 32-33 0.2952860952397103 1107 34-35 0.22270025257181914 1107 36-37 0.30473193436577617 1107 38-39 0.2646507730170029 1107 40-41 0.1751397971513029 1107 42-43 0.15471540390793592 1107 44-45 -0.020988613001954093 1107 46-47 0.35169971942773515 1107 48-49 0.2892652026779956 1107 50-51 0.250690421077838 1107 52-53 0.4297666779877112 1107 54-55 0.3362931600451837 1107 56-57 0.2428486012156661 1107 58-59 0.1609097587000754 1107 60-61 0.30197625769105585 1107 62-63 0.10316124073287014 1107 64-65 0.1882557982882176 1107 66-67 0.16478471390353988 1107 68-69 0.14473962118205463 1107 70-71 0.05442420991576569 1107 72-73 0.09142352891319305 1107 74-75 0.001684362094866998 1107 76-77 -0.1407486206153976 1107 78-79 -0.2881703095228936 1107 80-81 -0.3423882913793186 1107 82-83 -0.44504290848026784 1107 84-85 -0.4299323704377507 1107 86-87 -0.4536028327222219 1107 88-89 -0.5935045191859558 1107 90-91 -1.0682698018269505 1107 92-93 -4.319516471299593 1107 94-95 -2.1341461641582313 1107 96-97 -1.4234898128704394 1107 98-99 -1.1531057741540174 1107 100-101 -1.0712524511385215 1108 1 0.28226667369683156 1108 2 0.35650659686416475 1108 3 0.009357018985248544 1108 4 -0.27117899005716595 1108 5 -0.9369767768182271 1108 6 -0.1250930186429713 1108 7 0.055299863999294985 1108 8 0.194629877459775 1108 9 0.17679804695703183 1108 10-11 0.2196191887827439 1108 12-13 0.23437936963785688 1108 14-15 0.30496449615407784 1108 16-17 0.2621084840146608 1108 18-19 0.15499049780675023 1108 20-21 0.1398914436993195 1108 22-23 0.08680998398040884 1108 24-25 0.033963918215278 1108 26-27 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0.8235961230412947 2311 86-87 0.8185020500700695 2311 88-89 0.9737468213026226 2311 90-91 1.7120562418231557 2311 92-93 2.757084924715077 2311 94-95 2.1227271345150918 2311 96-97 1.5316752540093717 2311 98-99 1.3516759060662302 2311 100-101 1.3393181598765835 2312 1 0.19524379666752267 2312 2 0.055560851793700294 2312 3 -0.10381192498365266 2312 4 -0.07181638374104438 2312 5 -0.11335684754136821 2312 6 0.01112482650205493 2312 7 0.18079670824084815 2312 8 -0.6161619239207354 2312 9 -0.08312532407368423 2312 10-11 0.013601874230957378 2312 12-13 0.15244837421960966 2312 14-15 -0.2309812658826118 2312 16-17 0.06299855617322692 2312 18-19 0.13945088998237054 2312 20-21 0.18224823154512393 2312 22-23 0.0734923211316243 2312 24-25 0.2512410496059587 2312 26-27 0.17160638943503415 2312 28-29 0.01747060843374726 2312 30-31 0.18146630679608933 2312 32-33 0.4197097751323824 2312 34-35 0.14136938102643626 2312 36-37 0.36512535640976296 2312 38-39 0.3040410897401884 2312 40-41 0.5239464527708648 2312 42-43 0.44030930414447056 2312 44-45 0.28998323558995054 2312 46-47 0.4246154524426018 2312 48-49 0.40326157430865806 2312 50-51 0.33454710822276823 2312 52-53 0.39077395955592564 2312 54-55 0.4461915990811285 2312 56-57 0.4521396526897483 2312 58-59 0.47951954048097534 2312 60-61 0.6467092811028685 2312 62-63 0.46778496531871383 2312 64-65 0.645439004889127 2312 66-67 0.5503733225985208 2312 68-69 0.36052990680725827 2312 70-71 0.48614940306720555 2312 72-73 0.6010181113515394 2312 74-75 0.6893651242071037 2312 76-77 0.8487433140576712 2312 78-79 0.8989491014389799 2312 80-81 0.7653670326238036 2312 82-83 0.9563225127610373 2312 84-85 0.8748195966928201 2312 86-87 0.7636887039455154 2312 88-89 1.0451595131071016 2312 90-91 1.370575119778863 2312 92-93 2.415696743226423 2312 94-95 1.7706214333720034 2312 96-97 1.5453111767292391 2312 98-99 1.6847358711734906 2312 100-101 1.6435316275084375 2313 1 -0.19010429769880588 2313 2 -1.0116447609979637 2313 3 -0.2716878750896079 2313 4 -0.14562154871831012 2313 5 -0.7771935856253194 2313 6 -0.24464087036151483 2313 7 -1.200892735590287 2313 8 -1.0090651847062944 2313 9 -1.1112767227150186 2313 10-11 -2.1302595963121576 2313 12-13 -0.6709697116218649 2313 14-15 -0.2573633246983391 2313 16-17 -1.6280348891713814 2313 18-19 -1.199906250650038 2313 20-21 -1.5509691683858975 2313 22-23 -1.1898194289782538 2313 24-25 -0.4880045411756626 2313 26-27 -1.1522334402063095 2313 28-29 -2.425594901436739 2313 30-31 -0.8911509157164446 2313 32-33 -0.486585235578481 2313 34-35 -0.1755798516790108 2313 36-37 -0.306667412345206 2313 38-39 -0.5446034123056691 2313 40-41 -0.3795795376522335 2313 42-43 -0.1467752534109863 2313 44-45 -0.6175609719504465 2313 46-47 -0.721837619907916 2313 48-49 -0.29964852712441825 2313 50-51 -0.3612751364555429 2313 52-53 -0.11692424907113619 2313 54-55 -0.11834800688373193 2313 56-57 -0.42032379944936693 2313 58-59 -0.1705086642124236 2313 60-61 -0.27700792445224565 2313 62-63 -0.27935770890150025 2313 64-65 -0.09583582418120073 2313 66-67 -0.2379367836795332 2313 68-69 -0.3281472642331593 2313 70-71 -0.34150377033570933 2313 72-73 -0.16516555715044845 2313 74-75 -0.053101800166249546 2313 76-77 0.13263076291142895 2313 78-79 0.1034529699232074 2313 80-81 0.1258411252574767 2313 82-83 0.1015323868262179 2313 84-85 0.29960050563376583 2313 86-87 0.24128344922079847 2313 88-89 0.4611880846345464 2313 90-91 1.0781272869259872 2313 92-93 2.269031530053155 2313 94-95 1.523786761325102 2313 96-97 1.2274813115926655 2313 98-99 1.1656778041120752 2313 100-101 0.888776223916004 2314 1 -0.7606199013737083 2314 2 -0.8287996436154614 2314 3 -1.9282620738383827 2314 4 -0.5815317554544066 2314 5 -0.5194425849200144 2314 6 -2.980152626931364 2314 7 -4.048290214820497 2314 8 -1.6996381743649067 2314 9 -2.044811571074959 2314 10-11 -1.16205729354499 2314 12-13 -2.1281765072581464 2314 14-15 -3.22561612071933 2314 16-17 -2.310381371360201 2314 18-19 -2.7516677896752526 2314 20-21 -2.226035805741283 2314 22-23 -3.401275329246637 2314 24-25 -2.0630688885875657 2314 26-27 -2.0144508611803644 2314 28-29 -1.4810869873920538 2314 30-31 -1.4932762122641279 2314 32-33 -2.381100019453612 2314 34-35 -0.929038238826017 2314 36-37 -0.6529613678103772 2314 38-39 -0.6349144324296887 2314 40-41 -0.9901594200407331 2314 42-43 -0.8518308761382158 2314 44-45 -1.4766167824530427 2314 46-47 -1.8699876890642102 2314 48-49 -2.224092185354934 2314 50-51 -1.4345881350907348 2314 52-53 -0.973255713810584 2314 54-55 -0.5400710977578491 2314 56-57 -0.46637112800129543 2314 58-59 -0.5189705091740926 2314 60-61 -0.6810657043062385 2314 62-63 -0.721889745210845 2314 64-65 -0.9601936553299275 2314 66-67 -0.7146271054623909 2314 68-69 -0.6152846516824368 2314 70-71 -0.8073658653592055 2314 72-73 -0.6829584180226433 2314 74-75 -0.41129075256290193 2314 76-77 -0.4776557777167483 2314 78-79 -0.4862130876184949 2314 80-81 -0.5668844112762805 2314 82-83 -0.4505068078464589 2314 84-85 -0.43605941027950834 2314 86-87 -0.31495160889796736 2314 88-89 -0.039714954578442985 2314 90-91 0.6327030519961063 2314 92-93 1.4183065339460619 2314 94-95 0.9152927884563518 2314 96-97 0.8103684494381014 2314 98-99 0.5870972484801875 2314 100-101 0.7962879255272064 2315 1 -7.24764287409441 2315 2 -3.107969460120799 2315 3 -1.5290266451923173 2315 4 -0.9967508055554788 2315 5 -1.2983542532778927 2315 6 -1.831150394165526 2315 7 -3.1576021699138117 2315 8 -2.758146697258457 2315 9 -3.4078572016865216 2315 10-11 -2.3005385260514473 2315 12-13 -2.9920892676627844 2315 14-15 -4.68226051671736 2315 16-17 -4.4237877701689 2315 18-19 -3.1911400969713632 2315 20-21 -4.30109700040655 2315 22-23 -5.777746434829229 2315 24-25 -3.699417626403317 2315 26-27 -4.423448440810436 2315 28-29 -2.767477674281764 2315 30-31 -2.5052462923393435 2315 32-33 -1.7061067273007637 2315 34-35 -2.508585643204924 2315 36-37 -2.914211759807422 2315 38-39 -2.15496442629944 2315 40-41 -3.3194738595789275 2315 42-43 -7.729065374773235 2315 44-45 -4.311564003787051 2315 46-47 -2.922440242790138 2315 48-49 -5.568824507597753 2315 50-51 -6.775252814201497 2315 52-53 -6.996446458799028 2315 54-55 -4.523769180869234 2315 56-57 -3.716288800922051 2315 58-59 -4.014481026400706 2315 60-61 -3.0793531778368113 2315 62-63 -3.3519984138749273 2315 64-65 -1.9574245312654064 2315 66-67 -2.5747642951840284 2315 68-69 -2.0503617788324107 2315 70-71 -1.7756497238915507 2315 72-73 -2.046661433057462 2315 74-75 -2.5655229112297526 2315 76-77 -2.551085255832376 2315 78-79 -1.6098104852510495 2315 80-81 -1.436257829040695 2315 82-83 -1.891509405346671 2315 84-85 -1.8114172904827477 2315 86-87 -2.1370938503755355 2315 88-89 -1.3926673545860844 2315 90-91 -0.5895175272784812 2315 92-93 0.20793674134560902 2315 94-95 0.0590715644275015 2315 96-97 -0.20323334718674957 2315 98-99 -0.13001389199861535 2315 100-101 0.033353486531188636 2316 1 -4.511005484928237 2316 2 -2.9990387498486477 2316 3 -4.675297523406591 2316 4 -4.592040732093441 2316 5 -4.207430594064853 2316 6 -4.600120999559312 2316 7 -3.208250742489213 2316 8 -1.8650743058606416 2316 9 -2.1275105163214576 2316 10-11 -3.33057666538971 2316 12-13 -4.268689104891713 2316 14-15 -3.3328890358071703 2316 16-17 -3.189681421374992 2316 18-19 -3.4668066727460136 2316 20-21 -5.631443749968451 2316 22-23 -6.722842334465184 2316 24-25 -4.541753575811075 2316 26-27 -4.324998665924653 2316 28-29 -4.627509962078733 2316 30-31 -6.495984976403776 2316 32-33 -4.6349349890575695 2316 34-35 -6.354448686966137 2316 36-37 -9.476695098723205 2316 38-39 -6.290982628990349 2316 40-41 -5.637004473844712 2316 42-43 -4.730806432997035 2316 44-45 -3.670675749127202 2316 46-47 -6.089474463072261 2316 48-49 -3.5597538562550852 2316 50-51 -4.580637347611695 2316 52-53 -5.3920729211828125 2316 54-55 -7.224780377278318 2316 56-57 -5.179925548280391 2316 58-59 -6.156559891567614 2316 60-61 -6.61280630780956 2316 62-63 -6.943633293747407 2316 64-65 -5.747749731555512 2316 66-67 -6.903516475669573 2316 68-69 -5.891457365187826 2316 70-71 -6.595355286723642 2316 72-73 -8.554934839984973 2316 74-75 -8.19972129420783 2316 76-77 -7.288110915466866 2316 78-79 -6.185087009095277 2316 80-81 -6.372164874915914 2316 82-83 -5.202718597626937 2316 84-85 -6.521726555514075 2316 86-87 -5.281030411386521 2316 88-89 -5.329117706280403 2316 90-91 -5.811742767643459 2316 92-93 -4.544103353749161 2316 94-95 -4.4160108362194705 2316 96-97 -5.313457583513266 2316 98-99 -5.910537680375679 2316 100-101 -5.0283431202007804 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 22161.0 3 8914.0 4 9147.0 5 10352.0 6 11488.0 7 13363.0 8 15974.0 9 19130.0 10 22375.0 11 26116.0 12 29855.0 13 32660.0 14 35110.0 15 37931.0 16 41658.0 17 45600.0 18 45037.0 19 45530.0 20 48358.0 21 52652.0 22 57954.0 23 64371.0 24 71385.0 25 79649.0 26 89376.0 27 101488.0 28 114015.0 29 128200.0 30 146190.0 31 166603.0 32 190077.0 33 219669.0 34 258560.0 35 308105.0 36 351708.0 37 295387.0 38 87389.0 39 1491.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.12132918085451 18.64136537573998 13.020286336915271 24.21701910649025 2 15.703653001918646 19.84590246849829 40.0261069324203 24.424337597162765 3 19.4405072982576 24.06735063981654 29.202933840195477 27.289208221730384 4 12.770581584779075 16.14329417415773 37.31426548303771 33.771858758025495 5 13.961914782076198 37.103967974909075 34.758750311562004 14.175366931452727 6 32.651666462777264 35.93038973560188 17.532348769577926 13.885595032042936 7 28.833660403871548 31.818179065062807 21.333448413707888 18.014712117357753 8 26.03955283881938 35.35789398773229 19.822237834874997 18.78031533857333 9 26.395483941885008 16.239924974616553 20.063930250860825 37.30066083263762 10-11 25.071238099160524 25.98708621600509 27.975242044492184 20.966433640342196 12-13 26.035461117477332 23.380419798967463 27.89983622801131 22.6842828555439 14-15 23.009531908604643 24.90092254241569 25.71937532897108 26.37017022000859 16-17 22.860242224844157 27.82281705700933 26.378974190625478 22.937966527521038 18-19 22.7465718503551 27.051525554416333 27.976490351876997 22.22541224335157 20-21 23.499951160302487 26.340301382579902 27.853983691327056 22.305763765790555 22-23 23.13715535776274 26.16742626135833 27.771783546106683 22.92363483477225 24-25 23.045814466785533 26.32408702067355 27.89436450094604 22.73573401159488 26-27 22.994767285078428 26.442374992046396 27.903050851566025 22.659806871309147 28-29 22.701860060658763 26.77290534869699 27.86515696439555 22.6600776262487 30-31 23.03193397592756 26.517586502370037 27.80592611984346 22.644553401858943 32-33 22.956922101775316 26.47008652665092 27.79336134132973 22.77963003024403 34-35 23.102858374641873 26.548159396146755 27.743250270892013 22.605731958319364 36-37 23.162836060477705 26.375858121836487 27.7799390520651 22.681366765620712 38-39 22.931818415923054 26.693466441507358 27.785452745325795 22.589262397243797 40-41 23.069609232981676 26.54161108217124 27.75247579792878 22.636303886918302 42-43 22.931373183130013 26.622226308645498 27.58515963886857 22.86124086935592 44-45 23.026740972810707 26.569914190489392 27.518824551319483 22.884520285380425 46-47 23.153766937455895 26.69479085462972 27.339249633172646 22.812192574741736 48-49 23.143822418099763 26.723451153795686 27.302598983461014 22.830127444643534 50-51 22.92421762145284 26.873784630593615 27.351484567147793 22.850513180805752 52-53 23.04617855461274 27.007914622039557 27.082503952613195 22.863402870734507 54-55 23.049047303418156 26.776336865049526 27.147692897986346 23.026922933545972 56-57 22.992194119449803 26.988846772687193 27.095875216196696 22.92308389166631 58-59 23.113977379210723 26.805738299166045 27.15409651508482 22.926187806538415 60-61 23.028088743069077 26.877625722251132 27.30304290298565 22.791242631694146 62-63 22.894218454987953 26.837171716559716 27.323451916894314 22.945157911558013 64-65 22.92153543046523 26.86391460952483 27.250003066838453 22.96454689317149 66-67 22.83790919250417 26.90942364744078 27.022435438170994 23.230231721884056 68-69 22.76650050279327 26.896433371295903 27.204001383192693 23.133064742718133 70-71 22.952625986217008 26.6465554262101 27.130149213487915 23.270669374084978 72-73 22.909118458135094 26.671990060304857 27.136837479923635 23.282054001636414 74-75 22.832929936189906 27.042368520111363 26.907119853504103 23.21758169019463 76-77 22.888336647689815 26.832633029661295 26.96948094479922 23.309549377849674 78-79 22.836767742527968 26.894729137362123 26.982320741055275 23.286182379054633 80-81 22.8624233900545 26.889090622518324 26.877254348050123 23.371231639377058 82-83 22.960130177032337 26.779450590969113 26.954599138366746 23.305820093631805 84-85 22.924503830626954 26.791934016609787 26.810237146748072 23.473325006015184 86-87 22.77474622923477 26.82130544099915 27.00991567389218 23.394032655873904 88-89 22.86521012213781 26.66264855008329 26.858770426015326 23.613370901763567 90-91 22.584462818642578 26.70062163469553 27.155489978925917 23.559425567735975 92-93 22.316466674149847 26.73636365572145 27.51884255300509 23.42832711712361 94-95 22.844437089324394 26.657243424265047 26.91122238761139 23.587097098799166 96-97 22.78612703621324 26.60720172483697 26.871247285996308 23.73542395295348 98-99 22.769632243490708 26.598460355308916 26.6373145462784 23.99459285492197 100-101 22.930232085687486 26.580567616372967 26.705431756880603 23.78376854105894 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2640.0 1 1507.0 2 393.5 3 416.5 4 382.0 5 403.5 6 553.5 7 908.0 8 1455.0 9 1729.0 10 1610.0 11 1485.5 12 1553.5 13 1719.0 14 1913.0 15 2158.5 16 2406.0 17 2657.0 18 2984.5 19 3402.0 20 3886.5 21 4674.5 22 5847.5 23 7292.0 24 9051.5 25 11554.0 26 15421.0 27 20037.5 28 24397.5 29 29156.5 30 34427.0 31 39790.5 32 45334.5 33 51227.5 34 58021.0 35 64983.0 36 72555.0 37 78782.0 38 83041.5 39 87303.5 40 90778.5 41 94159.5 42 98064.5 43 103025.5 44 108646.5 45 113597.5 46 118703.0 47 125129.5 48 132180.5 49 134291.0 50 131505.5 51 129536.0 52 128762.0 53 127093.5 54 123170.5 55 116513.5 56 107238.0 57 97121.5 58 87569.5 59 77450.5 60 66271.0 61 54338.5 62 42769.5 63 33160.0 64 25006.0 65 18308.5 66 13078.0 67 9315.5 68 6584.5 69 4607.0 70 3246.0 71 2132.5 72 1363.5 73 875.5 74 562.0 75 341.0 76 224.0 77 151.0 78 96.5 79 68.0 80 52.0 81 40.0 82 33.0 83 27.5 84 22.0 85 16.0 86 9.0 87 6.0 88 5.5 89 6.0 90 5.5 91 7.5 92 7.0 93 3.5 94 2.5 95 1.5 96 2.5 97 2.5 98 0.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08786612397837476 2 0.09751808456690836 3 0.09512778711708343 4 0.09697345983150521 5 0.09431085001397871 6 0.07818390646009656 7 0.09040770607692279 8 0.08662558985884537 9 0.08105831478583539 10-11 0.08766945393503474 12-13 0.08701892994552543 14-15 0.09276774659700311 16-17 0.08992359520100889 18-19 0.09272236120238618 20-21 0.1031912588940245 22-23 0.11213218163355952 24-25 0.13412896955789783 26-27 0.14033164015554483 28-29 0.13912136296576005 30-31 0.14087626489094798 32-33 0.13742697490006137 34-35 0.1318748282919237 36-37 0.13633772542925507 38-39 0.12871297913361096 40-41 0.12473419287219352 42-43 0.12479470673168276 44-45 0.11559660008931846 46-47 0.13400794183891937 48-49 0.1514964472313094 50-51 0.16225278575552157 52-53 0.17030112906759035 54-55 0.16638285666566213 56-57 0.13626208310489352 58-59 0.12886426378233407 60-61 0.12558138690504286 62-63 0.12255569393058091 64-65 0.10839545081009903 66-67 0.12238928081698551 68-69 0.12010488262126674 70-71 0.12769937198716622 72-73 0.15515753573040833 74-75 0.16792596008263774 76-77 0.17382606138283851 78-79 0.17019522981348417 80-81 0.1769425251465343 82-83 0.17635251501651425 84-85 0.15267646749134955 86-87 0.15434059862730362 88-89 0.17561122023777104 90-91 0.2662458532877785 92-93 0.5341860946412558 94-95 0.17364451980437082 96-97 0.18654910034045097 98-99 0.2057319937985397 100-101 0.21311468465622682 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3305028.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.573919492321195 #Duplication Level Percentage of deduplicated Percentage of total 1 77.95990044041349 48.00296633347397 2 12.297121783614577 15.14363973383106 3 4.2247127364276675 7.803963657329435 4 1.934155288878171 4.76374088172127 5 1.0636679238075015 3.2747101553543767 6 0.6284195456936221 2.3216552708364087 7 0.4076893795298685 1.757212312313255 8 0.28787438107705154 1.418044317147213 9 0.20607976068428158 1.1420224734033695 >10 0.9278832205534259 9.834748981777478 >50 0.04505128147499806 1.8826904675932823 >100 0.016600636760975857 1.7573003963465987 >500 4.4662292845079913E-4 0.1840993925671765 >1k 3.473733864096769E-4 0.5037448247833045 >5k 4.9624769487096686E-5 0.20946080152187013 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6855 0.20741125339936603 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4992 0.15104259328514008 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3334 0.10087660376856111 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.025692974461941E-5 2 0.0 0.0 0.0 0.0 3.025692974461941E-5 3 0.0 0.0 0.0 0.0 3.025692974461941E-5 4 0.0 0.0 0.0 3.025692974461941E-5 3.025692974461941E-5 5 0.0 0.0 0.0 3.025692974461941E-5 3.025692974461941E-5 6 0.0 0.0 0.0 3.025692974461941E-5 9.077078923385823E-5 7 0.0 0.0 0.0 3.025692974461941E-5 9.077078923385823E-5 8 3.025692974461941E-5 0.0 0.0 6.051385948923882E-5 9.077078923385823E-5 9 3.025692974461941E-5 0.0 0.0 1.2102771897847764E-4 9.077078923385823E-5 10-11 3.025692974461941E-5 0.0 0.0 1.5128464872309706E-4 9.077078923385823E-5 12-13 3.025692974461941E-5 0.0 0.0 1.8154157846771645E-4 1.8154157846771645E-4 14-15 3.025692974461941E-5 0.0 0.0 2.1179850821233587E-4 2.269269730846456E-4 16-17 3.025692974461941E-5 0.0 0.0 3.176977623185038E-4 2.420554379569553E-4 18-19 3.025692974461941E-5 0.0 0.0 3.782116218077426E-4 3.025692974461941E-4 20-21 3.025692974461941E-5 0.0 0.0 4.841108759139106E-4 3.328262271908135E-4 22-23 3.025692974461941E-5 0.0 0.0 6.807809192539367E-4 4.2359701642467174E-4 24-25 3.025692974461941E-5 3.025692974461941E-5 0.0 0.0011951487249124667 4.992393407862203E-4 26-27 3.025692974461941E-5 3.025692974461941E-5 0.0 0.0015279749521032802 5.294962705308397E-4 28-29 3.025692974461941E-5 6.051385948923882E-5 0.0 0.003025692974461941 5.748816651477687E-4 30-31 3.025692974461941E-5 6.051385948923882E-5 0.0 0.007518847041537923 5.900101300200784E-4 32-33 3.025692974461941E-5 6.051385948923882E-5 0.0 0.01703465144622073 6.505239895093173E-4 34-35 3.025692974461941E-5 6.051385948923882E-5 0.0 0.0316487485128719 6.959093841262464E-4 36-37 3.025692974461941E-5 7.564232436154853E-5 0.0 0.055445823757015074 6.959093841262464E-4 38-39 3.025692974461941E-5 9.077078923385823E-5 0.0 0.0905589907256459 7.261663138708658E-4 40-41 3.025692974461941E-5 9.077078923385823E-5 0.0 0.1290458053608018 7.261663138708658E-4 42-43 3.025692974461941E-5 9.077078923385823E-5 0.0 0.1684705848180409 7.412947787431756E-4 44-45 3.025692974461941E-5 9.077078923385823E-5 0.0 0.21598909298196567 7.564232436154853E-4 46-47 3.025692974461941E-5 9.077078923385823E-5 0.0 0.27627602549811986 8.320655679770338E-4 48-49 3.025692974461941E-5 9.077078923385823E-5 0.0 0.3376824644148249 9.984786815724405E-4 50-51 3.025692974461941E-5 9.077078923385823E-5 0.0 0.40237178020882125 0.0010589925410616793 52-53 3.025692974461941E-5 9.077078923385823E-5 0.0 0.47040448673959795 0.001119506400550918 54-55 3.025692974461941E-5 9.077078923385823E-5 0.0 0.5450029470249571 0.0011346348654232277 56-57 3.025692974461941E-5 9.077078923385823E-5 0.0 0.635319882312646 0.0011648917951678473 58-59 3.025692974461941E-5 9.077078923385823E-5 0.0 0.73438107029653 0.001180020260040157 60-61 3.025692974461941E-5 9.077078923385823E-5 0.0 0.8435480728151168 0.0012102771897847765 62-63 3.025692974461941E-5 9.077078923385823E-5 0.0 0.9538648386639992 0.0012405341195293959 64-65 3.025692974461941E-5 1.0589925410616793E-4 0.0 1.0749681999668383 0.0012405341195293959 66-67 3.025692974461941E-5 1.2102771897847764E-4 0.0 1.2149821423600646 0.0012405341195293959 68-69 3.025692974461941E-5 1.2102771897847764E-4 0.0 1.372832544837744 0.0012405341195293959 70-71 3.025692974461941E-5 1.2102771897847764E-4 0.0 1.542377250661719 0.0012556625844017057 72-73 3.025692974461941E-5 1.2102771897847764E-4 0.0 1.7169597352881731 0.0013010479790186347 74-75 3.025692974461941E-5 1.2102771897847764E-4 0.0 1.8976238627932955 0.001331304908763254 76-77 3.025692974461941E-5 1.2102771897847764E-4 0.0 2.1019337808938383 0.0013464333736355636 78-79 3.025692974461941E-5 1.2102771897847764E-4 0.0 2.3339287897107077 0.0013615618385078734 80-81 3.025692974461941E-5 1.2102771897847764E-4 0.0 2.58218689826531 0.0013615618385078734 82-83 3.025692974461941E-5 1.2102771897847764E-4 0.0 2.831685540939441 0.0014220756979971122 84-85 3.025692974461941E-5 1.2102771897847764E-4 0.0 3.0802613472563625 0.0014220756979971122 86-87 3.025692974461941E-5 1.3615618385078737E-4 0.0 3.3563255742462696 0.001437204162869422 88-89 3.025692974461941E-5 1.5128464872309706E-4 0.0 3.6584864031409112 0.0014523326277417316 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 11730 0.0 28.685741 1 GGTATCA 7955 0.0 26.190405 1 ATCAACG 13330 0.0 24.816107 3 TCAACGC 13350 0.0 24.778177 4 CAACGCA 13535 0.0 24.508888 5 TATCAAC 13830 0.0 24.056555 2 AACGCAG 13950 0.0 23.810184 6 GTACATG 8700 0.0 22.802103 1 TACATGG 8805 0.0 21.992186 2 ACGCAGA 15545 0.0 21.000841 7 ACATGGG 8810 0.0 20.90195 3 GTATAGG 1045 0.0 20.89101 1 CGCAGAG 15595 0.0 20.538895 8 AGAGTAC 10455 0.0 20.061504 10-11 GAGTACT 6845 0.0 18.891146 12-13 AGTACTT 7260 0.0 18.366861 12-13 GCAGAGT 16230 0.0 18.215235 9 GTACTTT 7380 0.0 17.490444 14-15 CATGGGG 5775 0.0 17.25779 4 TATACCG 275 5.9703416E-7 17.257267 5 >>END_MODULE