FastQCFastQC Report
Fri 27 May 2016
SRR939597_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939597_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3305028
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71370.21594370758734874No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57970.17539942172955872No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39410.1192425601235451No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT34390.10405358139174617No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75600.030.3213121
GTATCAA111100.026.9029851
TCAACGC125550.023.6484184
ATCAACG127000.023.3784163
CAACGCA128500.023.0689015
AACGCAG130300.022.8610696
TACATGG88450.022.5035862
GTACATG91300.022.4263881
TATCAAC133350.022.3367462
ACATGGG87500.020.8109743
CGCAGAG143950.020.171058
ACGCAGA146650.020.0559837
AGAGTAC108400.019.00576810-11
GAGTACT75600.018.79960312-13
GTACTTT79350.017.67431314-15
GCAGAGT157000.017.6262869
GAGTACA69800.017.3307971
CATGGGG56950.016.1939144
AGTACAT65600.015.9959562
AGTACTT81250.015.84427212-13
Produced by FastQC (version 0.11.5)