##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939597_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3305028 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.55197353849952 31.0 31.0 34.0 30.0 34.0 2 31.77000043569979 31.0 31.0 34.0 30.0 34.0 3 31.81931439007476 31.0 31.0 34.0 30.0 34.0 4 35.59996677789114 37.0 35.0 37.0 33.0 37.0 5 35.323783943736636 37.0 35.0 37.0 33.0 37.0 6 35.340589550224685 37.0 35.0 37.0 32.0 37.0 7 35.27308422197936 37.0 35.0 37.0 32.0 37.0 8 35.231322699837946 37.0 35.0 37.0 32.0 37.0 9 36.85859786967009 39.0 37.0 39.0 33.0 39.0 10-11 36.73864729739052 39.0 35.0 39.0 32.0 39.0 12-13 36.6821960358581 39.0 35.0 39.0 32.0 39.0 14-15 37.77742306570474 40.0 37.0 41.0 32.0 41.0 16-17 37.73132512039232 39.5 37.0 41.0 32.0 41.0 18-19 37.681698309363796 39.5 37.0 41.0 32.0 41.0 20-21 37.605800313945906 39.0 36.5 41.0 32.0 41.0 22-23 37.47292579669522 39.0 36.0 41.0 32.0 41.0 24-25 37.34646136129558 39.0 36.0 41.0 31.5 41.0 26-27 37.089375642203336 39.0 36.0 40.0 31.0 41.0 28-29 36.89099759517922 39.0 36.0 40.0 30.5 41.0 30-31 36.677140102897766 38.5 35.5 40.0 30.0 41.0 32-33 36.530818649645326 38.0 35.0 40.0 30.0 41.0 34-35 36.53470227786269 38.0 35.0 40.0 30.0 41.0 36-37 36.454563168602505 38.5 35.0 40.0 30.0 41.0 38-39 36.51502165791031 39.0 35.0 40.0 30.0 41.0 40-41 36.43558042473468 39.0 35.0 40.0 30.0 41.0 42-43 36.27444926336479 38.5 35.0 40.0 30.0 41.0 44-45 36.085062516868234 38.0 35.0 40.0 29.5 41.0 46-47 35.83618338483063 38.0 35.0 40.0 28.5 41.0 48-49 35.580487517806205 38.0 34.0 40.0 27.5 41.0 50-51 35.333613966356715 38.0 34.0 40.0 27.0 41.0 52-53 35.0987814626684 38.0 34.0 40.0 27.0 41.0 54-55 34.79866509451659 38.0 34.0 40.0 26.0 41.0 56-57 32.707668739871494 37.0 30.5 39.5 16.5 41.0 58-59 27.601233030400955 32.5 18.0 38.5 7.5 40.0 60-61 28.356846598576475 33.0 20.0 38.0 9.5 40.0 62-63 31.44841132964683 34.5 29.5 38.5 15.5 40.0 64-65 31.66661734787118 35.0 31.0 39.0 10.5 40.0 66-67 24.89342525993728 27.5 18.0 35.5 2.0 38.5 68-69 17.367591590752028 13.0 7.5 28.0 2.0 35.0 70-71 16.21248624822543 12.5 7.5 26.5 2.0 33.5 72-73 17.30113284970657 13.5 7.0 28.5 2.0 34.0 74-75 18.31299447387435 17.0 8.5 29.0 2.0 34.5 76-77 19.563160281849353 21.5 8.0 30.0 2.0 34.0 78-79 22.675606379129015 27.5 10.5 33.5 2.0 35.5 80-81 26.338725723352418 31.5 22.5 34.5 2.0 36.0 82-83 27.676226646188777 33.0 26.5 35.0 2.0 36.0 84-85 27.73640828458942 34.0 27.0 35.0 2.0 36.0 86-87 27.50473687363617 34.0 27.0 35.0 2.0 36.0 88-89 27.085977032569772 33.0 26.0 35.0 2.0 35.0 90-91 25.72784088364758 32.5 21.0 35.0 2.0 35.0 92-93 23.17885309897526 31.5 2.0 34.5 2.0 35.0 94-95 15.764328471649861 4.5 2.0 32.0 2.0 34.5 96-97 13.184015233758988 2.0 2.0 30.0 2.0 34.0 98-99 12.839378062757714 2.0 2.0 29.5 2.0 34.0 100-101 12.546236370765996 2.0 2.0 30.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3955793223053412 1101 2 0.4076292179785739 1101 3 0.37273734351078147 1101 4 0.31475021977917095 1101 5 0.4154134341400848 1101 6 0.4838894048023974 1101 7 0.4034118668929594 1101 8 0.4096045810440643 1101 9 0.4787849382823097 1101 10-11 0.521617740458872 1101 12-13 0.5315364558541233 1101 14-15 0.7289902232338576 1101 16-17 0.6998114948607039 1101 18-19 0.6109326713177197 1101 20-21 0.5516127260750991 1101 22-23 0.59299139158054 1101 24-25 0.5395027802291494 1101 26-27 0.5211064563842598 1101 28-29 0.6002729665365152 1101 30-31 0.6630693571729651 1101 32-33 0.6179265503383249 1101 34-35 0.5222938316045216 1101 36-37 0.3903914484925224 1101 38-39 0.46077430514935 1101 40-41 0.47909448213165007 1101 42-43 0.5650943922242888 1101 44-45 0.507402779305778 1101 46-47 0.6202704442211981 1101 48-49 0.574872831278725 1101 50-51 0.4982308150074033 1101 52-53 0.3240625120974485 1101 54-55 0.4750320264864385 1101 56-57 -1.684455896300637 1101 58-59 -9.436941737728972 1101 60-61 -9.771541298418999 1101 62-63 -3.518470518369142 1101 64-65 -5.003892259844456 1101 66-67 -9.438354036467295 1101 68-69 -4.500214790353649 1101 70-71 -4.258845047955122 1101 72-73 -4.526824417485853 1101 74-75 -6.614465719835803 1101 76-77 -8.051797957411416 1101 78-79 -11.292820819317956 1101 80-81 -8.044513992789128 1101 82-83 -4.09501503215122 1101 84-85 -3.1786894587920607 1101 86-87 -2.9882554685181084 1101 88-89 -3.178528206477818 1101 90-91 -9.422476330225884 1101 92-93 -16.77401336603902 1101 94-95 -10.95857397778913 1101 96-97 -9.13373433303645 1101 98-99 -9.235768736951066 1101 100-101 -9.058857123538608 1102 1 0.41117467000890784 1102 2 0.3802128280776387 1102 3 0.40465002504041436 1102 4 0.30527833038649277 1102 5 0.2850147914634604 1102 6 0.37057640710442996 1102 7 0.42619206883850325 1102 8 0.4549999520154344 1102 9 0.49936180598381696 1102 10-11 0.43523702293679634 1102 12-13 0.4217199450869771 1102 14-15 0.4821488796184781 1102 16-17 0.4313412973844706 1102 18-19 0.48274328511910625 1102 20-21 0.5939418275116566 1102 22-23 0.6797348788340543 1102 24-25 0.4784619857759438 1102 26-27 0.5379289567672316 1102 28-29 0.6366393654129254 1102 30-31 0.5229535788150699 1102 32-33 0.5503828139824947 1102 34-35 0.3501809469817445 1102 36-37 0.4177790168548441 1102 38-39 0.5506838844812307 1102 40-41 0.365610858292996 1102 42-43 0.5944983496452707 1102 44-45 0.6477192555923637 1102 46-47 0.4980606060482131 1102 48-49 0.5098014301939813 1102 50-51 0.4368756157334417 1102 52-53 0.3465810829071003 1102 54-55 0.31479762973044245 1102 56-57 -2.94176906561178 1102 58-59 -10.56538262146315 1102 60-61 -10.774058511062993 1102 62-63 -4.269493288174278 1102 64-65 -5.360003755242747 1102 66-67 -6.676245107896804 1102 68-69 -4.249121216951377 1102 70-71 -4.138315018543992 1102 72-73 -4.523230608510273 1102 74-75 -5.248632416771663 1102 76-77 -7.4248303938816385 1102 78-79 -9.95406640462155 1102 80-81 -5.6191710559612 1102 82-83 -3.094350623303349 1102 84-85 -2.579597982123822 1102 86-87 -2.499380121804208 1102 88-89 -2.7981208778580573 1102 90-91 -8.814519598330417 1102 92-93 -13.40303857685739 1102 94-95 -10.508554276490816 1102 96-97 -9.149115493278416 1102 98-99 -9.23999221100194 1102 100-101 -9.136918615804698 1103 1 0.24449984061001118 1103 2 0.16546857554810046 1103 3 0.18590331280755734 1103 4 0.21460307289764557 1103 5 0.23029183459534153 1103 6 0.1842384627343634 1103 7 0.19606015908566832 1103 8 0.2011172123848226 1103 9 0.346251623610236 1103 10-11 0.39116688511359854 1103 12-13 0.32062632036223704 1103 14-15 0.4671439638397388 1103 16-17 0.4405901264858798 1103 18-19 0.4418724848448292 1103 20-21 0.3247140582593957 1103 22-23 0.4236200854237353 1103 24-25 0.4027957254319503 1103 26-27 0.4473511985800158 1103 28-29 0.44028166723085604 1103 30-31 0.49888122798446943 1103 32-33 0.41714162882632877 1103 34-35 0.3961489200483541 1103 36-37 0.35568239187253425 1103 38-39 0.3335556606554846 1103 40-41 0.42980122409223753 1103 42-43 0.3430676040794509 1103 44-45 0.36303625983335763 1103 46-47 0.2637362263251717 1103 48-49 0.17822761914128193 1103 50-51 0.27493018269142055 1103 52-53 0.3168551330371514 1103 54-55 0.42485076004755484 1103 56-57 -2.850232186387565 1103 58-59 -10.321933667562632 1103 60-61 -10.609709153379324 1103 62-63 -4.337848461351179 1103 64-65 -5.517161292346902 1103 66-67 -6.596623550302379 1103 68-69 -4.180620928534838 1103 70-71 -4.08715328035365 1103 72-73 -4.4650761297097965 1103 74-75 -5.053756707974415 1103 76-77 -6.770245477885222 1103 78-79 -9.346029922650306 1103 80-81 -5.249426948546901 1103 82-83 -2.9389154226996688 1103 84-85 -2.5291985524267133 1103 86-87 -2.3416911001348772 1103 88-89 -2.358409911136608 1103 90-91 -5.603333843567917 1103 92-93 -10.840105725306653 1103 94-95 -8.749135843953077 1103 96-97 -8.262105393257736 1103 98-99 -8.46030253994191 1103 100-101 -8.476311417363235 1104 1 0.19378724052873508 1104 2 0.18970364137467755 1104 3 0.13425577142065137 1104 4 0.17632091444190223 1104 5 0.2107330596448307 1104 6 0.179243893967012 1104 7 0.21528305227923283 1104 8 0.19976523550151626 1104 9 0.1739425219099786 1104 10-11 0.28312271947917367 1104 12-13 0.26246429244621083 1104 14-15 0.3138038635804392 1104 16-17 0.2507983617326772 1104 18-19 0.2930709252127528 1104 20-21 0.19310619422531516 1104 22-23 0.3123302510664274 1104 24-25 0.45241455332959646 1104 26-27 0.45271540451671655 1104 28-29 0.35793725305625657 1104 30-31 0.37090746196924584 1104 32-33 0.3367018208880239 1104 34-35 0.42020957557582506 1104 36-37 0.32155606600437636 1104 38-39 0.38322818034342987 1104 40-41 0.27815008271479513 1104 42-43 0.2982279616526924 1104 44-45 0.23190854123537719 1104 46-47 0.31580850498098556 1104 48-49 0.3056904329554939 1104 50-51 0.26621103203569163 1104 52-53 0.26957726719603414 1104 54-55 0.1775628776068814 1104 56-57 -2.8961026231685167 1104 58-59 -10.2000565603249 1104 60-61 -10.283622539367581 1104 62-63 -4.1166901727602685 1104 64-65 -5.481961086663336 1104 66-67 -6.627341855796519 1104 68-69 -4.221061937056781 1104 70-71 -4.096092391310904 1104 72-73 -4.357470080282191 1104 74-75 -5.034379076801631 1104 76-77 -6.5701503465951525 1104 78-79 -9.237419440657344 1104 80-81 -5.0511778026204475 1104 82-83 -2.797618875108192 1104 84-85 -2.2597680436424277 1104 86-87 -2.1069060552172942 1104 88-89 -2.1702788070512007 1104 90-91 -4.800870781099103 1104 92-93 -7.048106620639263 1104 94-95 -8.268257074822406 1104 96-97 -7.968634385372172 1104 98-99 -8.11320659244861 1104 100-101 -8.19577911871471 1105 1 0.25095925861428725 1105 2 0.23285546238813737 1105 3 0.18764145340518112 1105 4 0.15227778376540613 1105 5 0.14214274229839674 1105 6 0.17330340603609073 1105 7 0.1355826278267429 1105 8 0.18681175784518445 1105 9 0.20212117531825413 1105 10-11 0.3136207971834679 1105 12-13 0.2868791351391806 1105 14-15 0.35872634388949365 1105 16-17 0.3387114037325105 1105 18-19 0.3649775662056598 1105 20-21 0.38183772761635026 1105 22-23 0.43950232179751936 1105 24-25 0.4879278089149679 1105 26-27 0.4525480175986871 1105 28-29 0.5110722904997473 1105 30-31 0.466974866208524 1105 32-33 0.4160663927024544 1105 34-35 0.3707091774115767 1105 36-37 0.29541889434531754 1105 38-39 0.3636305337888288 1105 40-41 0.32318562147326446 1105 42-43 0.36604615883301506 1105 44-45 0.30258806653142045 1105 46-47 0.3438489854293394 1105 48-49 0.22434902120757982 1105 50-51 0.2519550951509615 1105 52-53 0.26438486372271086 1105 54-55 0.20268027931313526 1105 56-57 1.3428770476277165 1105 58-59 -8.524118065157026 1105 60-61 -9.843574543898203 1105 62-63 -3.9743957145316102 1105 64-65 -5.423329837843003 1105 66-67 -6.889997519373239 1105 68-69 -4.039212642588971 1105 70-71 -4.1399354654926945 1105 72-73 -2.478861368930165 1105 74-75 -4.483685264714957 1105 76-77 -6.658008455997241 1105 78-79 -9.343140267240614 1105 80-81 -5.247558387394999 1105 82-83 -2.8167932429108014 1105 84-85 -2.266323923385176 1105 86-87 -2.1079586639029273 1105 88-89 -2.1499263874666106 1105 90-91 -4.897786382621405 1105 92-93 -7.014149687968503 1105 94-95 -8.391956352465218 1105 96-97 -8.386703429908312 1105 98-99 -8.754387554406858 1105 100-101 -8.546998557656545 1106 1 0.20754752806512045 1106 2 0.21935171396690833 1106 3 0.177043482780892 1106 4 0.10783854992448738 1106 5 0.15926552894175927 1106 6 0.1981886057924811 1106 7 0.21303513037187827 1106 8 0.1549941241799715 1106 9 0.19173651180668116 1106 10-11 0.30163410936187773 1106 12-13 0.3138674938822419 1106 14-15 0.31569411476306897 1106 16-17 0.36136699128255856 1106 18-19 0.3393416804074647 1106 20-21 0.3039455213308955 1106 22-23 0.399038898155041 1106 24-25 0.44319436814365076 1106 26-27 0.2876660613957327 1106 28-29 0.4378311149119867 1106 30-31 0.45278729695695574 1106 32-33 0.35150578645283304 1106 34-35 0.4555443553741654 1106 36-37 0.4507559951907183 1106 38-39 0.4289601135974337 1106 40-41 0.3254264330118488 1106 42-43 0.18393698339495046 1106 44-45 0.3890392089011172 1106 46-47 0.4666333605746402 1106 48-49 0.4724308638483663 1106 50-51 0.4349322897191854 1106 52-53 0.4478532222540679 1106 54-55 0.40011949765455057 1106 56-57 1.7778257027060462 1106 58-59 -8.594489659945719 1106 60-61 -9.894439737205293 1106 62-63 -3.702439541818819 1106 64-65 -5.240100540163052 1106 66-67 -7.321536473958577 1106 68-69 -4.192584142490494 1106 70-71 -4.109912891041237 1106 72-73 -4.603687519380387 1106 74-75 -5.170098905860755 1106 76-77 -6.751482713788668 1106 78-79 -9.327379546219099 1106 80-81 -5.10866809662064 1106 82-83 -2.4904358176873735 1106 84-85 -2.0124353923708718 1106 86-87 -1.8404053409051144 1106 88-89 -1.715005659837015 1106 90-91 -4.142026381759479 1106 92-93 -6.459785624801622 1106 94-95 -7.56615407388783 1106 96-97 -7.401661957410658 1106 98-99 -6.908538140344579 1106 100-101 -7.3989813781644225 1107 1 -0.012208369224648408 1107 2 -0.07388320466239762 1107 3 -0.05614209414398985 1107 4 -0.01908520476925446 1107 5 -0.07146392966801329 1107 6 0.020870604881388033 1107 7 0.09581296334870615 1107 8 0.14637413591550086 1107 9 0.13067877713832132 1107 10-11 -0.006707769409750597 1107 12-13 -0.026622276900049258 1107 14-15 0.036979426794978565 1107 16-17 0.02399247799760218 1107 18-19 0.04956293070976159 1107 20-21 0.03202103551006985 1107 22-23 0.0036349691905996906 1107 24-25 0.1859146857902445 1107 26-27 0.19893535424586872 1107 28-29 0.14142713533104967 1107 30-31 0.12461757986563526 1107 32-33 0.041635477148574296 1107 34-35 -0.051153331077294695 1107 36-37 0.003330593586369446 1107 38-39 -0.1564519796193906 1107 40-41 -0.08732048641232382 1107 42-43 -0.14655542131950483 1107 44-45 -0.1723085863757987 1107 46-47 -0.04288722627669728 1107 48-49 -0.17121095512082007 1107 50-51 -0.0021440549453757285 1107 52-53 -0.06287058418097757 1107 54-55 -0.24573885490992353 1107 56-57 1.0053059107210771 1107 58-59 -8.936251357510315 1107 60-61 -10.535223130497297 1107 62-63 -4.383319162682561 1107 64-65 -5.903643075824245 1107 66-67 -7.148741290773749 1107 68-69 -4.22255585349658 1107 70-71 -4.159644014371125 1107 72-73 -2.677028376448792 1107 74-75 -4.9683882090966485 1107 76-77 -7.102692354167878 1107 78-79 -7.806116871006369 1107 80-81 -2.96424167518774 1107 82-83 -1.878074145993427 1107 84-85 -1.6523029552618596 1107 86-87 -1.5954633811470984 1107 88-89 -1.4416542107993884 1107 90-91 -1.0207357268758344 1107 92-93 -4.030303909540002 1107 94-95 -6.348851482885944 1107 96-97 -6.966481247167568 1107 98-99 -6.694466428026212 1107 100-101 -7.393664584550349 1108 1 -0.13176622974300045 1108 2 -0.07100190881486057 1108 3 -0.11565961735165686 1108 4 -0.09466907965294524 1108 5 -0.12734635717119147 1108 6 -0.10279945037981264 1108 7 -0.17988129263653718 1108 8 -0.09355855488566789 1108 9 -0.30840868367659624 1108 10-11 -0.18931297951410642 1108 12-13 -0.12387667167622851 1108 14-15 -0.15354138831293085 1108 16-17 -0.22189094276925658 1108 18-19 -0.2384770109578156 1108 20-21 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11.99739389803593 2316 96-97 13.91819621088922 2316 98-99 13.589214976190375 2316 100-101 12.145695644187159 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 15.0 4 44.0 5 104.0 6 237.0 7 664.0 8 1427.0 9 2523.0 10 3987.0 11 6496.0 12 9747.0 13 14118.0 14 17449.0 15 24191.0 16 33482.0 17 45062.0 18 52089.0 19 55395.0 20 58794.0 21 59070.0 22 58235.0 23 50116.0 24 50430.0 25 58848.0 26 82959.0 27 123606.0 28 175335.0 29 239086.0 30 296933.0 31 322911.0 32 322760.0 33 293311.0 34 259161.0 35 231852.0 36 188227.0 37 119633.0 38 43708.0 39 3019.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.28701269695042 19.363015841983284 13.848434353491768 24.50153710757453 2 15.992628925717364 20.58109240773297 38.89379419694324 24.532484469606423 3 19.215120527367937 24.196032879709847 29.830497089727526 26.75834950319469 4 13.201128704507193 16.873170212173694 36.812668455456354 33.11303262786276 5 13.754483178215443 37.328190915445525 34.90035883605377 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27.40706019193387 22.90705678428605 52-53 22.98740769880473 26.93776106516556 27.143448541941684 22.93138269408803 54-55 23.007436312465927 26.668439055682036 27.206363159178448 23.117761472673592 56-57 21.995744064570463 26.802885200492653 28.3102920129102 22.891078722026688 58-59 21.56831464286779 26.25849800646305 29.543891778807925 22.629295571861235 60-61 22.733054327130716 26.694202307884158 27.870240825339277 22.70250253964585 62-63 22.901130502943168 26.757894026563633 27.382853083030987 22.95812238746221 64-65 22.46871330232395 26.828001509219735 27.6983929475634 23.004892240892918 66-67 22.18821445985794 26.25647013613694 28.73773631051349 22.817579093491634 68-69 22.22106636525793 25.741668252509342 29.492593673468814 22.54467170876391 70-71 22.17370631390014 25.70417736077331 29.405686692340822 22.716429632985726 72-73 21.406889643319484 25.941266785739863 29.831657510396635 22.82018606054401 74-75 21.990827359230376 26.230973948588755 28.909764802499556 22.86843388968132 76-77 20.359990528987943 26.80021734837135 29.294398860041245 23.54539326259946 78-79 21.217212938887076 26.74480132637932 28.616081020678024 23.42190471405558 80-81 22.389609540370746 26.80105013829081 27.306895407582505 23.50244491375594 82-83 22.800908013346373 26.694926728829728 27.12132386590994 23.38284139191396 84-85 22.785087609815786 26.657858862425638 26.98107895450668 23.5759745732519 86-87 22.595881024036007 26.68522025808917 27.182403255895082 23.536495461979737 88-89 22.673147107644066 26.602515807761645 27.0238443193419 23.700492765252395 90-91 22.06135518288637 26.676150646652236 27.528996030226395 23.733498140235003 92-93 21.1154595467147 26.77676425661876 28.241107160611286 23.86666903605526 94-95 20.129308714715833 26.52730386621789 29.36606005129436 23.977327367771924 96-97 20.05965374721705 26.544723878581788 29.082062114181383 24.313560260019774 98-99 20.447122400363277 26.600572384687077 28.453510126304298 24.498795088645352 100-101 20.950559814464718 26.589816421051214 28.034156524469484 24.42546724001458 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 235.0 1 192.0 2 147.5 3 175.5 4 210.5 5 243.0 6 323.5 7 481.5 8 673.5 9 865.5 10 1029.5 11 1182.0 12 1375.5 13 1613.5 14 1899.0 15 2167.0 16 2410.0 17 2695.5 18 3100.0 19 3643.0 20 4283.5 21 5153.0 22 6313.0 23 7732.0 24 9693.0 25 12448.5 26 15950.0 27 20086.5 28 24698.5 29 29773.5 30 35260.5 31 41261.0 32 47846.0 33 54943.5 34 62018.0 35 69123.0 36 76371.0 37 82966.0 38 88417.5 39 93425.0 40 98295.5 41 102706.5 42 107505.5 43 113130.5 44 118480.0 45 123696.0 46 128955.5 47 133737.5 48 137216.5 49 137754.0 50 135997.5 51 134008.0 52 131384.0 53 126168.5 54 119186.0 55 110588.5 56 99931.5 57 88301.0 58 76675.0 59 64792.0 60 52934.0 61 41921.5 62 32130.0 63 23862.0 64 17175.5 65 12219.0 66 8407.5 67 5627.5 68 3779.5 69 2479.0 70 1542.0 71 899.0 72 547.5 73 334.0 74 167.5 75 86.0 76 47.5 77 22.0 78 13.0 79 6.5 80 3.0 81 2.5 82 1.0 83 1.0 84 1.5 85 1.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023297835903356944 2 0.006596010684327031 3 3.328262271908135E-4 4 0.0 5 0.14024086936631097 6 0.0 7 6.051385948923882E-5 8 0.0011800202600401569 9 0.0 10-11 0.0 12-13 0.0 14-15 7.71551708487795E-4 16-17 0.0 18-19 2.1179850821233587E-4 20-21 0.0 22-23 3.025692974461941E-4 24-25 1.5128464872309705E-5 26-27 3.176977623185038E-4 28-29 0.003192106088057348 30-31 0.012496111984527816 32-33 0.0027533806067603665 34-35 0.0038577585424389746 36-37 0.10748774291776046 38-39 0.10721543055005889 40-41 0.11886434850173735 42-43 0.12161772910849772 44-45 0.11710944657654943 46-47 0.11120934527634864 48-49 0.11093703290864707 50-51 0.10984778343784078 52-53 0.0886830610814795 54-55 0.07617182063207936 56-57 0.5544279806404061 58-59 1.566249968230224 60-61 0.519450969855626 62-63 0.005189063451202229 64-65 0.12055873656743603 66-67 1.8950217668352582 68-69 2.854045412020715 70-71 2.7660885172531064 72-73 2.619024710229384 74-75 1.9984096957726227 76-77 1.4119244980677923 78-79 0.9334716680161257 80-81 0.055793778449078194 82-83 3.176977623185038E-4 84-85 1.3615618385078734E-4 86-87 2.269269730846456E-4 88-89 1.8154157846771645E-4 90-91 0.24933222956053624 92-93 0.3945352354049648 94-95 1.993341660040399 96-97 2.4078767260065574 98-99 1.419186161206501 100-101 0.9392204846676034 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3305028.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.32702571056147 #Duplication Level Percentage of deduplicated Percentage of total 1 73.08792525176044 40.43737519535745 2 14.525251637846774 16.07277941639047 3 5.195944008262024 8.624283832072525 4 2.4963269785749995 5.52457387702349 5 1.3631874817247527 3.771055442485047 6 0.8425964025182722 2.7971011695453027 7 0.5361612381530503 2.0764944625810196 8 0.36159419963015693 1.600474526378207 9 0.266101439432031 1.3250341062966056 >10 1.2331328784026656 11.86608511795535 >50 0.06540137062493424 2.4712722274299077 >100 0.025283003883532124 2.3784874377127565 >500 6.017600533511677E-4 0.23571864495572398 >1k 3.829382146382722E-4 0.42779241400536056 >5k 1.0941091846807778E-4 0.3914721298107636 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7137 0.21594370758734874 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5797 0.17539942172955872 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3941 0.1192425601235451 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3439 0.10405358139174617 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 3.025692974461941E-5 0.0 4 0.0 0.0 0.0 3.025692974461941E-5 0.0 5 0.0 0.0 0.0 3.025692974461941E-5 0.0 6 0.0 0.0 0.0 6.051385948923882E-5 0.0 7 0.0 0.0 0.0 6.051385948923882E-5 0.0 8 0.0 0.0 0.0 6.051385948923882E-5 0.0 9 0.0 0.0 0.0 1.2102771897847764E-4 6.051385948923882E-5 10-11 0.0 0.0 0.0 1.5128464872309706E-4 6.051385948923882E-5 12-13 0.0 0.0 0.0 2.1179850821233587E-4 1.2102771897847764E-4 14-15 0.0 0.0 0.0 2.7231236770157473E-4 1.6641311359540676E-4 16-17 0.0 0.0 0.0 4.0846855155236204E-4 2.723123677015747E-4 18-19 0.0 0.0 0.0 4.3872548129698143E-4 2.723123677015747E-4 20-21 0.0 0.0 0.0 5.446247354031494E-4 3.328262271908135E-4 22-23 0.0 0.0 0.0 7.412947787431756E-4 3.933400866800523E-4 24-25 0.0 3.025692974461941E-5 0.0 0.0012556625844017057 4.5385394616929113E-4 26-27 0.0 3.025692974461941E-5 0.0 0.0014825895574863512 5.294962705308397E-4 28-29 0.0 3.025692974461941E-5 0.0 0.0030559499042065604 5.446247354031494E-4 30-31 0.0 3.025692974461941E-5 0.0 0.007776030944367188 5.59753200275459E-4 32-33 0.0 3.025692974461941E-5 0.0 0.017700303900602357 5.748816651477687E-4 34-35 0.0 3.025692974461941E-5 0.0 0.03269261258906127 5.748816651477687E-4 36-37 0.0 3.025692974461941E-5 0.0 0.05723098261194762 5.748816651477687E-4 38-39 0.0 3.025692974461941E-5 0.0 0.09393263839217095 5.900101300200784E-4 40-41 0.0 3.025692974461941E-5 0.0 0.13384152872532395 6.051385948923882E-4 42-43 0.0 3.025692974461941E-5 0.0 0.1747337692751771 6.051385948923882E-4 44-45 0.0 3.025692974461941E-5 0.0 0.22424923480224676 6.353955246370076E-4 46-47 0.0 3.025692974461941E-5 0.0 0.28400667104787014 6.807809192539367E-4 48-49 0.0 3.025692974461941E-5 0.0 0.33615448946272164 6.959093841262464E-4 50-51 0.0 3.025692974461941E-5 1.5128464872309705E-5 0.3853522572274728 6.959093841262464E-4 52-53 0.0 3.025692974461941E-5 3.025692974461941E-5 0.43638056924177343 6.959093841262464E-4 54-55 0.0 3.025692974461941E-5 3.025692974461941E-5 0.4930971840480625 7.110378489985561E-4 56-57 0.0 4.538539461692911E-5 3.025692974461941E-5 0.54318753124028 7.412947787431756E-4 58-59 0.0 6.051385948923882E-5 3.025692974461941E-5 0.5772417056678492 7.564232436154853E-4 60-61 0.0 6.051385948923882E-5 3.025692974461941E-5 0.6044578139731343 7.564232436154853E-4 62-63 1.5128464872309705E-5 6.051385948923882E-5 3.025692974461941E-5 0.6288600278121699 8.169371031047241E-4 64-65 3.025692974461941E-5 6.051385948923882E-5 3.025692974461941E-5 0.6543514911220116 8.169371031047241E-4 66-67 3.025692974461941E-5 7.564232436154853E-5 3.025692974461941E-5 0.6766054629491792 8.471940328493435E-4 68-69 3.025692974461941E-5 9.077078923385823E-5 3.025692974461941E-5 0.7082844683917957 8.471940328493435E-4 70-71 3.025692974461941E-5 9.077078923385823E-5 3.025692974461941E-5 0.7557575911611036 8.471940328493435E-4 72-73 3.025692974461941E-5 9.077078923385823E-5 3.025692974461941E-5 0.8208705039715245 8.774509625939629E-4 74-75 3.025692974461941E-5 9.077078923385823E-5 3.025692974461941E-5 0.9060437612026282 8.925794274662726E-4 76-77 4.538539461692911E-5 9.077078923385823E-5 3.025692974461941E-5 1.0488110842026148 9.077078923385823E-4 78-79 6.051385948923882E-5 9.077078923385823E-5 3.025692974461941E-5 1.267553557791341 9.077078923385823E-4 80-81 6.051385948923882E-5 9.077078923385823E-5 3.025692974461941E-5 1.5245559190421383 9.379648220832018E-4 82-83 6.051385948923882E-5 1.0589925410616793E-4 3.025692974461941E-5 1.7846142301971422 9.83350216700131E-4 84-85 6.051385948923882E-5 1.2102771897847764E-4 3.025692974461941E-5 1.984597407344204 9.984786815724405E-4 86-87 6.051385948923882E-5 1.2102771897847764E-4 3.025692974461941E-5 2.141585487324162 0.0010589925410616793 88-89 6.051385948923882E-5 1.2102771897847764E-4 3.025692974461941E-5 2.2958655720919765 0.0010892494708062987 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7560 0.0 30.321312 1 GTATCAA 11110 0.0 26.902985 1 TCAACGC 12555 0.0 23.648418 4 ATCAACG 12700 0.0 23.378416 3 CAACGCA 12850 0.0 23.068901 5 AACGCAG 13030 0.0 22.861069 6 TACATGG 8845 0.0 22.503586 2 GTACATG 9130 0.0 22.426388 1 TATCAAC 13335 0.0 22.336746 2 ACATGGG 8750 0.0 20.810974 3 CGCAGAG 14395 0.0 20.17105 8 ACGCAGA 14665 0.0 20.055983 7 AGAGTAC 10840 0.0 19.005768 10-11 GAGTACT 7560 0.0 18.799603 12-13 GTACTTT 7935 0.0 17.674313 14-15 GCAGAGT 15700 0.0 17.626286 9 GAGTACA 6980 0.0 17.330797 1 CATGGGG 5695 0.0 16.193914 4 AGTACAT 6560 0.0 15.995956 2 AGTACTT 8125 0.0 15.844272 12-13 >>END_MODULE