FastQCFastQC Report
Fri 27 May 2016
SRR939596_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939596_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3224012
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA91550.033.879141
GGTATCA65850.032.1288531
GTACATG69750.027.8292431
TATCAAC118650.025.479682
ATCAACG122500.024.2554763
TCAACGC123750.023.8170154
TACATGG81050.023.5456182
CAACGCA126200.023.4094725
ACATGGG77950.023.1495463
AACGCAG130550.022.5936586
GGACCGT3350.022.5402416
ACGCAGA137600.021.0930847
GAGTACT65650.020.34346812-13
CGCAGAG145450.019.6302158
AGAGTAC98200.019.48613210-11
GTGTAGG14800.019.4303261
TGGACCG5650.019.211275
GAGTACA55650.019.1934031
CATGGGG51750.018.619494
GTACTTT73100.018.27399314-15
Produced by FastQC (version 0.11.5)