FastQCFastQC Report
Fri 27 May 2016
SRR939596_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939596_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3224012
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70260.2179272285587026No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56040.1738206929750882No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39500.12251815439892903No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT33040.10248100813520546No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA76150.028.2028521
GTATCAA106950.025.7299731
GTACATG85100.022.1861521
TCAACGC123250.022.0118834
ATCAACG123900.021.8964063
CAACGCA125450.021.6258645
TACATGG84950.021.4959962
AACGCAG127750.021.259926
ACATGGG81350.020.9375533
TATCAAC130400.020.878932
AGAGTAC104850.019.3827610-11
ACGCAGA142650.018.5434767
CGCAGAG142150.018.3765078
GAGTACT71400.017.76482212-13
AGTACTT76300.017.52004812-13
ACCGTGC6950.016.961158
CATGGGG52250.016.7958854
GTACTTT75000.016.7863714-15
GAGTACA64500.016.4654791
TAGATCG2355.4429787E-516.0619565
Produced by FastQC (version 0.11.5)