##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939596_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3224012 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.47257020135161 31.0 31.0 34.0 30.0 34.0 2 31.685636095647286 31.0 31.0 34.0 30.0 34.0 3 31.744092453750174 31.0 31.0 34.0 30.0 34.0 4 35.53909942022548 37.0 35.0 37.0 33.0 37.0 5 35.285073690792714 37.0 35.0 37.0 33.0 37.0 6 35.282860919872505 37.0 35.0 37.0 32.0 37.0 7 35.189156554007866 37.0 35.0 37.0 32.0 37.0 8 35.145681839893896 37.0 35.0 37.0 32.0 37.0 9 36.76127353123996 39.0 35.0 39.0 32.0 39.0 10-11 36.63655904506559 39.0 35.0 39.0 32.0 39.0 12-13 36.573404193284645 39.0 35.0 39.0 32.0 39.0 14-15 37.673369081752796 39.0 36.5 41.0 32.0 41.0 16-17 37.61376942765722 39.0 36.0 41.0 32.0 41.0 18-19 37.56019534046399 39.0 36.0 41.0 32.0 41.0 20-21 37.48695290216041 39.0 36.0 41.0 32.0 41.0 22-23 37.34899792556603 39.0 36.0 41.0 31.5 41.0 24-25 37.217707781484684 39.0 36.0 40.5 31.0 41.0 26-27 36.934975273044884 39.0 36.0 40.0 31.0 41.0 28-29 36.74379282707385 39.0 36.0 40.0 30.0 41.0 30-31 36.520309787928824 38.0 35.0 40.0 30.0 41.0 32-33 36.37307801583865 38.0 35.0 40.0 30.0 41.0 34-35 36.36208953316551 38.0 35.0 40.0 30.0 41.0 36-37 36.3120611833951 38.0 35.0 40.0 30.0 41.0 38-39 36.38318902038826 38.0 35.0 40.0 30.0 41.0 40-41 36.29260545556282 38.0 35.0 40.0 30.0 41.0 42-43 36.132413123772494 38.0 35.0 40.0 29.5 41.0 44-45 35.9435993104244 38.0 35.0 40.0 29.0 41.0 46-47 35.70395783266315 38.0 34.5 40.0 28.0 41.0 48-49 35.44019439133601 38.0 34.0 40.0 27.0 41.0 50-51 35.20760484142119 38.0 34.0 40.0 27.0 41.0 52-53 34.98565079782581 38.0 34.0 40.0 26.0 41.0 54-55 34.6976807158286 38.0 33.0 40.0 26.0 41.0 56-57 33.94013933570967 37.5 32.5 40.0 23.5 41.0 58-59 30.824227856472 35.5 27.5 39.0 8.5 40.5 60-61 30.537222411082837 34.5 26.5 39.0 10.5 40.0 62-63 32.2754299611788 35.5 30.5 39.0 20.5 40.0 64-65 32.311296452990874 35.5 31.0 39.0 19.0 40.0 66-67 27.42861022229446 32.0 19.0 37.0 5.0 39.5 68-69 19.800852788389125 20.5 8.0 32.0 2.0 37.0 70-71 17.83110236562395 15.5 7.5 29.0 2.0 35.0 72-73 19.54773989674976 20.0 7.5 31.0 2.0 35.5 74-75 21.033089361950267 23.5 9.5 32.5 2.0 36.0 76-77 22.955916882443365 27.5 12.0 33.0 2.0 35.5 78-79 25.19941427017021 30.5 16.5 34.5 2.0 36.0 80-81 26.81210569315499 32.0 23.0 35.0 2.0 36.0 82-83 27.878953614316572 33.0 26.5 35.0 2.0 36.0 84-85 27.91073777020681 34.0 27.0 35.0 2.0 36.0 86-87 27.63309209146864 33.5 27.0 35.0 2.0 36.0 88-89 27.201476607407166 33.0 26.0 35.0 2.0 35.0 90-91 25.407742744133706 32.5 16.0 34.5 2.0 35.0 92-93 23.840030837354206 31.5 4.5 34.0 2.0 35.0 94-95 20.002919499058937 28.0 2.0 34.0 2.0 35.0 96-97 17.264432018243106 18.5 2.0 33.0 2.0 35.0 98-99 17.529390089118777 21.5 2.0 33.0 2.0 35.0 100-101 16.730986423127458 17.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.29092140256654986 1101 2 0.32291501859196003 1101 3 0.2523436337926164 1101 4 0.2666177419845681 1101 5 0.35196128922838454 1101 6 0.354815160496436 1101 7 0.3315011805281216 1101 8 0.3599263294629509 1101 9 0.4860166733446647 1101 10-11 0.43788899212109555 1101 12-13 0.3939380490797433 1101 14-15 0.5151485175662742 1101 16-17 0.49881478485953323 1101 18-19 0.5003748876008345 1101 20-21 0.5470494533786692 1101 22-23 0.50827444873687 1101 24-25 0.43030153609071675 1101 26-27 0.43757232638991894 1101 28-29 0.45336024193439783 1101 30-31 0.3184885799217412 1101 32-33 0.2803776234248474 1101 34-35 0.231105335854366 1101 36-37 0.17799746498727842 1101 38-39 0.08434260416029105 1101 40-41 0.23182975512011694 1101 42-43 0.2953465266700448 1101 44-45 0.301610436891103 1101 46-47 0.32400872616134535 1101 48-49 0.2123316306794365 1101 50-51 0.15261234679949354 1101 52-53 0.10448594199336725 1101 54-55 0.26400906171775773 1101 56-57 -3.3252370971128684 1101 58-59 -10.48558669113434 1101 60-61 -11.898304030055055 1101 62-63 -3.922354260570316 1101 64-65 -0.9858993760270991 1101 66-67 -1.743298823355584 1101 68-69 -4.248542857614286 1101 70-71 -4.352600545080657 1101 72-73 -3.265473273933784 1101 74-75 -6.219062946780388 1101 76-77 -4.744272776986904 1101 78-79 1.542868267048 1101 80-81 2.2856905782575936 1101 82-83 0.8487188481924228 1101 84-85 0.499504558021723 1101 86-87 0.4798252200546429 1101 88-89 0.3176630591129346 1101 90-91 1.7883705295006465 1101 92-93 1.3924243615478957 1101 94-95 -8.42764404252573 1101 96-97 -9.28466143790725 1101 98-99 -8.653254944295062 1101 100-101 -9.812701359868617 1102 1 0.23223852946242474 1102 2 0.34132109069821936 1102 3 0.2604110249719618 1102 4 0.15134989787421915 1102 5 0.28893222468128954 1102 6 0.23155954198465167 1102 7 0.3211837307419856 1102 8 0.2972431555843329 1102 9 0.285617262508822 1102 10-11 0.3472189727034447 1102 12-13 0.30297986115760267 1102 14-15 0.3528888607145859 1102 16-17 0.42059616603370387 1102 18-19 0.4111253641957049 1102 20-21 0.3666245651738933 1102 22-23 0.37841749354785037 1102 24-25 0.2332774343227797 1102 26-27 0.10034153062022 1102 28-29 0.34288400090787263 1102 30-31 0.3188882119153078 1102 32-33 0.19488158878269957 1102 34-35 0.21860616519579423 1102 36-37 0.03701561318948876 1102 38-39 0.1805420088037195 1102 40-41 0.13197527689752064 1102 42-43 0.3987220568956644 1102 44-45 0.18860061306533993 1102 46-47 0.216417487545435 1102 48-49 0.3336788792562828 1102 50-51 0.24630868840854703 1102 52-53 0.24566971078624533 1102 54-55 0.032100647551281725 1102 56-57 -3.7005399726054975 1102 58-59 -10.200475470918757 1102 60-61 -8.235207070089022 1102 62-63 -2.4149344191846254 1102 64-65 -0.762082901546485 1102 66-67 -1.888977622734572 1102 68-69 -4.860678666144782 1102 70-71 -4.77752944287483 1102 72-73 -3.6126013198284355 1102 74-75 -4.159692715493648 1102 76-77 -4.247786309627568 1102 78-79 1.7305776481160144 1102 80-81 2.100508808730474 1102 82-83 0.6029418547873426 1102 84-85 0.3457658868257454 1102 86-87 0.22306354403300332 1102 88-89 0.10846665779129694 1102 90-91 1.412534885069178 1102 92-93 0.7143559088115126 1102 94-95 -9.251981122270779 1102 96-97 -10.106469800322824 1102 98-99 -9.22871329366233 1102 100-101 -9.853516016432799 1103 1 0.127932354104356 1103 2 0.11387836045044253 1103 3 0.19115011290600137 1103 4 0.11272437192751994 1103 5 0.136017948681463 1103 6 0.21524010639453195 1103 7 0.21853508439067326 1103 8 0.3127678272660859 1103 9 0.33209182581367713 1103 10-11 0.2745405758315016 1103 12-13 0.21637099550245864 1103 14-15 0.34165123905620476 1103 16-17 0.34872953336763146 1103 18-19 0.19948696052364312 1103 20-21 0.2684724975127111 1103 22-23 0.2501519489008146 1103 24-25 0.40532809520934876 1103 26-27 0.3232422063820408 1103 28-29 0.18304677875648423 1103 30-31 0.15403845204451017 1103 32-33 0.02172590855829526 1103 34-35 0.04451801865323546 1103 36-37 0.11582814240875194 1103 38-39 0.14220803795922876 1103 40-41 0.17863216839312202 1103 42-43 0.12389397240418987 1103 44-45 0.07211669959685452 1103 46-47 0.04170628009931221 1103 48-49 0.010004264950531194 1103 50-51 -0.12299982449042801 1103 52-53 0.03407083280588097 1103 54-55 0.024767144214969505 1103 56-57 0.5397265070578996 1103 58-59 -8.287231389147326 1103 60-61 -7.818840458673325 1103 62-63 -2.197996597772754 1103 64-65 -0.3846822470350304 1103 66-67 -1.8099143391271078 1103 68-69 -3.551591582120814 1103 70-71 -3.8618942692592917 1103 72-73 -3.082903925124027 1103 74-75 -3.8194105105550626 1103 76-77 -0.6759660027722028 1103 78-79 3.2595487051095127 1103 80-81 2.7006915619286573 1103 82-83 1.1582735730924156 1103 84-85 0.6593333992572106 1103 86-87 0.46212045252890377 1103 88-89 0.27730893753119545 1103 90-91 1.6731426457049885 1103 92-93 1.2702235614954347 1103 94-95 -7.268227031574192 1103 96-97 -9.113843679365706 1103 98-99 -8.51735029480985 1103 100-101 -9.697366583817729 1104 1 0.2679077390288782 1104 2 0.25374724749738675 1104 3 0.20488381797720834 1104 4 0.1546036575879839 1104 5 0.21682924147527416 1104 6 0.15163676075366084 1104 7 0.19452885526953168 1104 8 0.23890351159030132 1104 9 0.3371881511345549 1104 10-11 0.23852093400596885 1104 12-13 0.23075549302838994 1104 14-15 0.32344035316465636 1104 16-17 0.2860866780188758 1104 18-19 0.2594216552213524 1104 20-21 0.283056678559106 1104 22-23 0.3395177339459039 1104 24-25 0.4156134291455018 1104 26-27 0.2626059820332145 1104 28-29 0.42999551409418757 1104 30-31 0.3647284276806104 1104 32-33 0.2810447240205818 1104 34-35 0.3526369747730911 1104 36-37 0.32944027914800955 1104 38-39 0.17591235564440666 1104 40-41 0.1983837570701823 1104 42-43 0.1742986141041314 1104 44-45 0.20094019945870656 1104 46-47 0.24614603595794904 1104 48-49 0.07366136187867056 1104 50-51 0.21414558027685615 1104 52-53 0.22294557221260902 1104 54-55 0.20952965897145504 1104 56-57 0.5760473067966743 1104 58-59 -8.427425295660274 1104 60-61 -7.833111676617211 1104 62-63 -2.385669217268898 1104 64-65 -0.4417357220306357 1104 66-67 -1.5887757662991397 1104 68-69 -3.3184471318156348 1104 70-71 -3.7098738236761903 1104 72-73 -2.8005150069314766 1104 74-75 -3.640378536074362 1104 76-77 -0.2344373563501776 1104 78-79 3.7263128273811326 1104 80-81 3.010826442495304 1104 82-83 1.3866977059133525 1104 84-85 1.0674008646579622 1104 86-87 0.924889146171683 1104 88-89 0.7075445389679089 1104 90-91 2.3162107390413382 1104 92-93 1.8871509470227714 1104 94-95 -6.875959430539353 1104 96-97 -8.347767272162029 1104 98-99 -6.276673129059969 1104 100-101 -5.780832537149555 1105 1 0.22603370611291496 1105 2 0.27166456801780114 1105 3 0.24307696827026248 1105 4 0.29111946968065183 1105 5 0.28776194245638465 1105 6 0.3281299031069125 1105 7 0.301346094992752 1105 8 0.2325110429065873 1105 9 0.2628184088308956 1105 10-11 0.2923306740709677 1105 12-13 0.2712567070091225 1105 14-15 0.33174364251890154 1105 16-17 0.39184304372654566 1105 18-19 0.35245457067829733 1105 20-21 0.3365281341239381 1105 22-23 0.5514640780711275 1105 24-25 0.4002585494453186 1105 26-27 0.3175696064404079 1105 28-29 0.5093272962158721 1105 30-31 0.5438740711477053 1105 32-33 0.5468324157074065 1105 34-35 0.5843905991415141 1105 36-37 0.35364353001696003 1105 38-39 0.4273155177763641 1105 40-41 0.2900211383376785 1105 42-43 0.4215906945129291 1105 44-45 0.4228559272984356 1105 46-47 0.3144492039394038 1105 48-49 0.17582568310051983 1105 50-51 0.1571275950212936 1105 52-53 0.1951971823827705 1105 54-55 0.14176938135143047 1105 56-57 0.7271820074955926 1105 58-59 -8.48273571752005 1105 60-61 -7.7712925547729235 1105 62-63 -2.1899205351329343 1105 64-65 -0.3454973428428332 1105 66-67 -1.5518382764872918 1105 68-69 -3.2161757665386723 1105 70-71 -3.802398111298004 1105 72-73 -2.836381213325563 1105 74-75 -3.5476373367392853 1105 76-77 -0.01156467994368171 1105 78-79 3.9413753756668477 1105 80-81 3.332444309715836 1105 82-83 1.6755870639979698 1105 84-85 1.2825636297795349 1105 86-87 1.250567101687544 1105 88-89 1.282088628222759 1105 90-91 2.560205188259218 1105 92-93 2.2214695492398135 1105 94-95 -6.1473292589839765 1105 96-97 -7.532129838299662 1105 98-99 -5.430081546633604 1105 100-101 -4.639479218775417 1106 1 0.10575300523291631 1106 2 0.11011844006441507 1106 3 0.10480507999833577 1106 4 0.0652098833553012 1106 5 0.16689646210795672 1106 6 0.1211086262603871 1106 7 0.10231674808854052 1106 8 0.12505162766248645 1106 9 0.12989658759897083 1106 10-11 0.09726609997075997 1106 12-13 0.1253627748580186 1106 14-15 0.1471249505559058 1106 16-17 0.26987030458617767 1106 18-19 0.14533959104623762 1106 20-21 0.10670694059122354 1106 22-23 0.13643175187295498 1106 24-25 0.05491756475615972 1106 26-27 0.015520487322355336 1106 28-29 0.160421859248558 1106 30-31 0.2716221010740014 1106 32-33 0.20955500612722489 1106 34-35 0.1689968900080956 1106 36-37 0.20301807023768248 1106 38-39 0.19685675803081182 1106 40-41 0.16026517474048774 1106 42-43 0.15558309322173614 1106 44-45 0.16756273018802403 1106 46-47 0.046234241162061096 1106 48-49 0.08978147963228622 1106 50-51 0.13140386518233527 1106 52-53 0.1540013378361067 1106 54-55 0.02727640602953585 1106 56-57 0.6416663449865965 1106 58-59 -8.556168224826425 1106 60-61 -7.902186085340659 1106 62-63 -2.295889503599124 1106 64-65 -0.4995193812665306 1106 66-67 0.5209980436889836 1106 68-69 -2.454160888927305 1106 70-71 -2.6075012003580813 1106 72-73 -1.2651003341324483 1106 74-75 -1.2565873923968844 1106 76-77 2.008472225315142 1106 78-79 4.202542508265637 1106 80-81 3.27895159521265 1106 82-83 1.5730376999730034 1106 84-85 1.0975348092273585 1106 86-87 0.9821647754338088 1106 88-89 0.8848683344051729 1106 90-91 2.2542358325218537 1106 92-93 1.7355736706301315 1106 94-95 -6.443200897785921 1106 96-97 -7.841615742555115 1106 98-99 -5.859012403743844 1106 100-101 -5.303717470693968 1107 1 0.049534132478235904 1107 2 0.1499997170814673 1107 3 0.11069334900003724 1107 4 0.0735758056793685 1107 5 0.07282656236947815 1107 6 0.025772004113193248 1107 7 0.04209530941954398 1107 8 0.1555834203264581 1107 9 0.08137339753852757 1107 10-11 0.044149259064859336 1107 12-13 0.03235822896370877 1107 14-15 0.06878038228235539 1107 16-17 0.1346633659372074 1107 18-19 0.05912681497516559 1107 20-21 0.07769396218739644 1107 22-23 0.027110954402353116 1107 24-25 -0.007565860554841208 1107 26-27 0.05544840833676545 1107 28-29 0.0050562667288645 1107 30-31 -0.03933859550712526 1107 32-33 0.09891453789401083 1107 34-35 0.06665691064682733 1107 36-37 0.05362653541447315 1107 38-39 0.018395835234535696 1107 40-41 0.006964073563068496 1107 42-43 -0.13628063609276353 1107 44-45 -0.20042841173779635 1107 46-47 -0.18315108984467798 1107 48-49 -0.302301163312201 1107 50-51 -0.15400127749143877 1107 52-53 -0.2251741034235195 1107 54-55 -0.14807539766747624 1107 56-57 0.30783735737394835 1107 58-59 -8.753667562662965 1107 60-61 -8.150239934599458 1107 62-63 -2.5846564340928104 1107 64-65 -0.6121404887796267 1107 66-67 0.3704905141446275 1107 68-69 -2.67273672077685 1107 70-71 -2.6067877779462503 1107 72-73 -1.215350555279386 1107 74-75 -0.8697712770441406 1107 76-77 2.2326120432519154 1107 78-79 4.342163361791453 1107 80-81 3.212093894181553 1107 82-83 1.6037301507161992 1107 84-85 1.1618257306407926 1107 86-87 0.9922753151147496 1107 88-89 0.8982448192047521 1107 90-91 2.1589773761546738 1107 92-93 1.7618791133253424 1107 94-95 -6.901830474068884 1107 96-97 -8.299886176540426 1107 98-99 -6.14718363435259 1107 100-101 -5.449280695313824 1108 1 -0.056553013630804116 1108 2 -0.05848528784998308 1108 3 -0.04080583437517049 1108 4 -0.048023572333647735 1108 5 -0.08135103387142806 1108 6 -0.032022355239881506 1108 7 -0.04191660369507133 1108 8 0.032169077943123625 1108 9 -0.07172175437244732 1108 10-11 -0.10967044719605923 1108 12-13 -0.011343974871927287 1108 14-15 -0.142417728724908 1108 16-17 -0.06027632610354061 1108 18-19 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2315 86-87 0.4196628730574048 2315 88-89 0.4682281332501077 2315 90-91 2.240372883502644 2315 92-93 3.6217038609772843 2315 94-95 6.18996786623228 2315 96-97 3.6500603440621546 2315 98-99 6.005531793311594 2315 100-101 6.347403526243067 2316 1 -0.5119532018673105 2316 2 -0.5238986387965312 2316 3 -0.5799583673079063 2316 4 -0.31336790008040794 2316 5 -0.5227637579922941 2316 6 -0.5463377070882842 2316 7 -0.4484401220877814 2316 8 -0.5588636645734297 2316 9 -0.5853417608106284 2316 10-11 -0.6187816433034428 2316 12-13 -0.614402125855726 2316 14-15 -0.657732084008579 2316 16-17 -0.7872472476222612 2316 18-19 -0.675348803033863 2316 20-21 -0.705430559294534 2316 22-23 -0.8882367742147039 2316 24-25 -0.7522141367310908 2316 26-27 -0.7442758239887084 2316 28-29 -0.6845775210691585 2316 30-31 -0.7200030490165759 2316 32-33 -0.6088775199302958 2316 34-35 -0.5900224535391203 2316 36-37 -0.47196806507239586 2316 38-39 -0.7593278556666192 2316 40-41 -0.8548465757024672 2316 42-43 -0.7117402416085596 2316 44-45 -0.663607193110856 2316 46-47 -0.7161710288150047 2316 48-49 -0.7141610225085415 2316 50-51 -0.5944291265107111 2316 52-53 -0.6442132316081484 2316 54-55 -0.511191314455111 2316 56-57 0.301434590221092 2316 58-59 3.3435840144873374 2316 60-61 3.3994924921060807 2316 62-63 0.7094737945665059 2316 64-65 0.09156461702946928 2316 66-67 -1.8236871665228058 2316 68-69 -2.768300692139295 2316 70-71 -2.9970840764118414 2316 72-73 -0.4446610154591042 2316 74-75 5.724664131757013 2316 76-77 5.888664309190464 2316 78-79 4.813375492743759 2316 80-81 3.142396584660414 2316 82-83 1.4716557122534546 2316 84-85 1.0521092911112326 2316 86-87 0.9030340718515006 2316 88-89 0.9248122295084329 2316 90-91 2.5011379020362554 2316 92-93 4.213751594644734 2316 94-95 6.820340042210397 2316 96-97 3.8032472194749545 2316 98-99 6.057749914185404 2316 100-101 6.098909918109136 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 21.0 4 43.0 5 99.0 6 260.0 7 674.0 8 1509.0 9 2698.0 10 4118.0 11 6419.0 12 9220.0 13 12857.0 14 16105.0 15 21490.0 16 28968.0 17 37635.0 18 43520.0 19 45708.0 20 48662.0 21 49869.0 22 50459.0 23 50700.0 24 53496.0 25 58031.0 26 71786.0 27 101009.0 28 142308.0 29 187362.0 30 226692.0 31 256359.0 32 268684.0 33 266783.0 34 274131.0 35 291757.0 36 291706.0 37 216965.0 38 80964.0 39 4939.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.15296513518276 19.459653660098436 13.851920704692738 24.53546050002606 2 15.99123091651006 20.638584587780485 38.86201070977213 24.50817378593733 3 19.182575455861716 24.187904233369437 29.87058949058376 26.758930820185086 4 13.202835473317096 16.896432147274886 36.76419318538516 33.13653919402285 5 13.712727505218785 37.33453672144193 34.95226898455438 14.000466788784902 6 32.750126240224915 36.43587554885032 17.0458732783873 13.768124932537473 7 28.5378590402269 31.970879760993448 21.283326488859224 18.207934709920433 8 25.91105486645513 35.75696105160964 19.666719396031652 18.66526468590358 9 25.890722491107354 16.029592941961752 20.000763024455246 38.07892154247565 10-11 24.922658476457283 26.142629121727833 27.984619784293606 20.950092617521275 12-13 25.71249734802476 23.421981679968933 28.04344400703223 22.822076964974077 14-15 22.616544809608946 25.036135280564864 25.73484892661157 26.61247098321462 16-17 22.580037323067863 27.944642877359467 26.496136877923664 22.97918292164901 18-19 22.51973012440431 27.189141204539446 28.023507415003206 22.267621256053033 20-21 23.235847137045397 26.40717528346669 27.922290611821545 22.434686967666373 22-23 22.872511020430444 26.29608698726928 27.78412425263926 23.047277739661016 24-25 22.832759720199206 26.453889072235555 27.91405914625522 22.799292061310023 26-27 22.818108941676503 26.58160591648381 27.869737976888715 22.73054716495097 28-29 22.590077008912125 26.82704826569044 27.82668303361298 22.75619169178445 30-31 22.90785957329531 26.57320271706569 27.76432679543731 22.754610914201688 32-33 22.84559938272323 26.507663615179116 27.779781083879072 22.866955918218583 34-35 23.026625253706595 26.541427977879934 27.76864905338962 22.66329771502385 36-37 23.007244914621268 26.388277505871866 27.83809860261125 22.76637897689562 38-39 22.749556262335098 26.768835492059907 27.842932076940603 22.638676168664393 40-41 22.990620957610382 26.575257246150734 27.78570410802243 22.648417688216455 42-43 22.86549551473729 26.6289966273606 27.60131577640245 22.90419208149966 44-45 22.944214425428747 26.556198553262924 27.586759286898104 22.91282773441023 46-47 23.011973706615084 26.724674906593414 27.396708716897532 22.86664266989397 48-49 23.0344680090241 26.713219162179243 27.371045283145374 22.881267545651284 50-51 22.843941174672164 26.873117012653424 27.417366604221804 22.865575208452608 52-53 22.975683120823405 26.933677909354202 27.133809871997478 22.95682909782491 54-55 22.984383999324738 26.687156163730613 27.210311736609004 23.118148100335645 56-57 22.784812091007524 26.912963933472707 27.369252378784896 22.932971596734873 58-59 22.01358779947062 26.440462627459816 28.853534105545364 22.692415467524203 60-61 22.2691581763613 26.736786347125253 28.31028542146891 22.683770055044537 62-63 22.8626494409158 26.765894328449136 27.38304425060943 22.98841198002564 64-65 22.849661772604055 26.868439414437084 27.28570828152691 22.996190531431953 66-67 22.599145774696037 26.333025549604095 28.21033902419594 22.857489651503926 68-69 21.754511328900893 25.75037893272274 30.026611474850164 22.4684982635262 70-71 22.685829702069622 25.64393192250043 29.090003566944368 22.58023480848558 72-73 22.026809532910146 25.95165628706765 29.216452151443693 22.805082028578514 74-75 22.178886604015148 26.27189515744498 28.63957528056736 22.909642957972515 76-77 21.155907442904937 26.70696128204439 28.718358732180548 23.418772542870126 78-79 22.392269497628085 26.582702759189175 27.814679438538807 23.210348304643933 80-81 22.52190766154544 26.773457046213288 27.250412771031773 23.454222521209495 82-83 22.79650757582102 26.686263398311482 27.132230171956824 23.384998853910677 84-85 22.771910082038836 26.697562408478266 26.95727398687515 23.573253522607747 86-87 22.58787030592229 26.73715149958622 27.13707941423518 23.537898780256306 88-89 22.652559187918403 26.63823107303294 26.987252835055465 23.721956903993195 90-91 22.457261642324962 26.515621987564902 27.481468491261246 23.545647878848897 92-93 22.39987412645357 26.60875531824268 27.300206391030702 23.691164164273047 94-95 21.264302845960632 26.486902556044107 28.450712781911562 23.798081816083698 96-97 20.87104521041355 26.35928730485557 28.72537626985112 24.04429121487976 98-99 21.6758059506026 26.31389498954953 27.897527632480596 24.112771427367278 100-101 20.952301925074003 26.42468297311959 28.347308168751166 24.27570693305524 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 313.0 1 241.5 2 187.0 3 232.5 4 251.5 5 266.5 6 341.0 7 537.5 8 846.0 9 1113.5 10 1244.0 11 1295.0 12 1451.0 13 1658.0 14 1868.5 15 2080.5 16 2291.5 17 2667.0 18 3046.5 19 3366.5 20 3883.5 21 4656.5 22 5684.5 23 7176.5 24 9121.5 25 11588.5 26 15120.5 27 19322.0 28 23579.5 29 28477.5 30 33980.0 31 39723.5 32 46005.0 33 52787.5 34 59288.5 35 66026.5 36 73027.0 37 79042.0 38 84443.5 39 89274.5 40 94016.0 41 98239.0 42 102329.5 43 107468.5 44 112690.5 45 117964.5 46 123275.0 47 128366.5 48 132876.0 49 134083.5 50 132357.5 51 130229.0 52 127960.0 53 123976.0 54 117924.5 55 110422.5 56 100804.0 57 89595.0 58 78110.0 59 66840.5 60 55325.0 61 44048.5 62 33852.0 63 25488.5 64 18547.0 65 12963.0 66 9102.0 67 6366.0 68 4321.0 69 2765.0 70 1720.0 71 1083.5 72 660.5 73 407.5 74 234.0 75 119.0 76 56.5 77 26.5 78 15.5 79 11.5 80 5.5 81 4.5 82 4.0 83 1.0 84 2.0 85 2.0 86 0.0 87 0.5 88 0.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025434148508132103 2 0.012841143271178891 3 2.4813803422567906E-4 4 0.0 5 0.061972474047863345 6 0.0 7 0.0 8 6.203450855641976E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 4.962760684513581E-4 16-17 1.550862713910494E-5 18-19 2.4813803422567906E-4 20-21 0.0 22-23 0.0 24-25 1.2406901711283953E-4 26-27 7.754313569552471E-5 28-29 0.001070095272598241 30-31 0.005164372837321945 32-33 0.005412510871547625 34-35 0.0019075611381099077 36-37 0.08430489712817446 38-39 0.07797737725541964 40-41 0.0831572587198807 42-43 0.08672424296187482 44-45 0.08999656328822597 46-47 0.08515787162082523 48-49 0.07493768633615507 50-51 0.0693700891932164 52-53 0.05856057607726026 54-55 0.0480147096226689 56-57 0.06657853630817752 58-59 0.7104502092423973 60-61 0.33766933869973187 62-63 0.0024503630879785807 64-65 0.002217733680892007 66-67 1.1050207009155053 68-69 2.378573652951664 70-71 2.3467344414350815 72-73 1.809763735370712 74-75 1.2868283368672324 76-77 0.7443520681684808 78-79 0.5666697270357555 80-81 0.03973310273038686 82-83 1.5508627139104942E-4 84-85 0.0015818799681887041 86-87 0.0017369662395797533 88-89 3.101725427820988E-5 90-91 0.33400930269490314 92-93 0.0536753585284422 94-95 0.9515783440012009 96-97 1.653700420469899 98-99 0.645376009766713 100-101 0.5697559438364374 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3224012.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.77168402517019 #Duplication Level Percentage of deduplicated Percentage of total 1 73.4769325497593 40.97932265303918 2 14.417540354827347 16.081810101791415 3 5.108701682309412 8.547626880138475 4 2.385408864902381 5.32153077936702 5 1.3389897077769697 3.7338855447546058 6 0.8168418369110688 2.7333986896046243 7 0.533119216770393 2.08130695538252 8 0.34804859858902965 1.5529005172728525 9 0.2712144026604351 1.3613475571427758 >10 1.2126955965583912 11.792988664776956 >50 0.06502223480593448 2.465154165660781 >100 0.024426437399339702 2.314212227627125 >500 5.571140689537947E-4 0.21684583366115112 >1k 3.8997984754560313E-4 0.4252403030846366 >5k 1.1142281358445803E-4 0.3924291266958177 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7026 0.2179272285587026 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5604 0.1738206929750882 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3950 0.12251815439892903 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3304 0.10248100813520546 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 3.101725427820988E-5 0.0 7 0.0 0.0 0.0 3.101725427820988E-5 0.0 8 0.0 0.0 0.0 3.101725427820988E-5 3.101725427820988E-5 9 0.0 0.0 0.0 6.203450855641976E-5 3.101725427820988E-5 10-11 0.0 0.0 0.0 9.305176283462964E-5 3.101725427820988E-5 12-13 0.0 0.0 0.0 1.7059489853015435E-4 1.861035256692593E-4 14-15 0.0 0.0 0.0 1.861035256692593E-4 2.3262940708657412E-4 16-17 0.0 0.0 0.0 2.4813803422567906E-4 2.63646661364784E-4 18-19 0.0 0.0 0.0 2.9466391564299385E-4 2.791552885038889E-4 20-21 0.0 0.0 0.0 3.566984241994137E-4 2.791552885038889E-4 22-23 0.0 0.0 0.0 4.4975018703404326E-4 3.1017254278209883E-4 24-25 0.0 0.0 0.0 6.203450855641977E-4 3.411897970603087E-4 26-27 3.101725427820988E-5 1.550862713910494E-5 0.0 0.0010235693911809262 3.566984241994137E-4 28-29 3.101725427820988E-5 3.101725427820988E-5 0.0 0.0022642595623093213 3.8771567847762354E-4 30-31 3.101725427820988E-5 3.101725427820988E-5 0.0 0.006854813195484384 4.187329327558334E-4 32-33 3.101725427820988E-5 3.101725427820988E-5 0.0 0.016315075750338397 4.3424155989493833E-4 34-35 3.101725427820988E-5 3.101725427820988E-5 0.0 0.030815642125401516 4.6525881417314825E-4 36-37 3.101725427820988E-5 3.101725427820988E-5 0.0 0.053427220494216526 4.962760684513581E-4 38-39 3.101725427820988E-5 4.652588141731482E-5 0.0 0.08732907942029992 5.11784695590463E-4 40-41 3.101725427820988E-5 6.203450855641976E-5 0.0 0.12313849948449324 5.583105770077778E-4 42-43 3.101725427820988E-5 6.203450855641976E-5 0.0 0.16239083477356786 5.583105770077778E-4 44-45 3.101725427820988E-5 6.203450855641976E-5 0.0 0.2093354491236385 6.048364584250927E-4 46-47 3.101725427820988E-5 6.203450855641976E-5 0.0 0.2664382142498229 7.754313569552471E-4 48-49 3.101725427820988E-5 6.203450855641976E-5 0.0 0.3247506522928575 8.374658655116668E-4 50-51 3.101725427820988E-5 6.203450855641976E-5 0.0 0.3841797114899076 8.374658655116668E-4 52-53 3.101725427820988E-5 6.203450855641976E-5 0.0 0.4428953738385589 8.839917469289817E-4 54-55 3.101725427820988E-5 6.203450855641976E-5 0.0 0.5123119889131926 8.995003740680866E-4 56-57 3.101725427820988E-5 7.754313569552471E-5 0.0 0.5792472236455695 9.150090012071915E-4 58-59 3.101725427820988E-5 9.305176283462965E-5 0.0 0.6295261928305478 9.305176283462965E-4 60-61 3.101725427820988E-5 9.305176283462965E-5 0.0 0.6713529602247138 9.305176283462965E-4 62-63 7.754313569552471E-5 9.305176283462965E-5 0.0 0.7089148551556259 9.615348826245064E-4 64-65 9.305176283462965E-5 9.305176283462965E-5 0.0 0.7539208911133085 9.615348826245064E-4 66-67 9.305176283462965E-5 9.305176283462965E-5 0.0 0.791994570739811 9.615348826245064E-4 68-69 1.085603899737346E-4 9.305176283462965E-5 0.0 0.8402884356509839 9.615348826245064E-4 70-71 1.2406901711283953E-4 1.5508627139104942E-4 0.0 0.9119227844065096 9.925521369027162E-4 72-73 1.2406901711283953E-4 1.5508627139104942E-4 0.0 1.0028963912044992 0.001054586645459136 74-75 1.2406901711283953E-4 1.5508627139104942E-4 0.0 1.126717270283113 0.001101112526876451 76-77 1.2406901711283953E-4 1.5508627139104942E-4 0.0 1.2939312880969425 0.0011166211540155557 78-79 1.2406901711283953E-4 1.5508627139104942E-4 0.0 1.5055309967828903 0.0011166211540155557 80-81 1.2406901711283953E-4 1.5508627139104942E-4 0.0 1.7556696439095139 0.0011321297811546607 82-83 1.2406901711283953E-4 1.5508627139104942E-4 0.0 2.0052189632048516 0.0012251815439892903 84-85 1.2406901711283953E-4 1.5508627139104942E-4 0.0 2.2290704873306924 0.0012406901711283953 86-87 1.5508627139104942E-4 1.5508627139104942E-4 0.0 2.43738236706315 0.0012406901711283953 88-89 1.5508627139104942E-4 1.5508627139104942E-4 0.0 2.6485013083077855 0.0012406901711283953 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7615 0.0 28.202852 1 GTATCAA 10695 0.0 25.729973 1 GTACATG 8510 0.0 22.186152 1 TCAACGC 12325 0.0 22.011883 4 ATCAACG 12390 0.0 21.896406 3 CAACGCA 12545 0.0 21.625864 5 TACATGG 8495 0.0 21.495996 2 AACGCAG 12775 0.0 21.25992 6 ACATGGG 8135 0.0 20.937553 3 TATCAAC 13040 0.0 20.87893 2 AGAGTAC 10485 0.0 19.38276 10-11 ACGCAGA 14265 0.0 18.543476 7 CGCAGAG 14215 0.0 18.376507 8 GAGTACT 7140 0.0 17.764822 12-13 AGTACTT 7630 0.0 17.520048 12-13 ACCGTGC 695 0.0 16.96115 8 CATGGGG 5225 0.0 16.795885 4 GTACTTT 7500 0.0 16.78637 14-15 GAGTACA 6450 0.0 16.465479 1 TAGATCG 235 5.4429787E-5 16.061956 5 >>END_MODULE