FastQCFastQC Report
Fri 27 May 2016
SRR939595_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939595_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2810274
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75520.26872824500386794No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52050.18521325678563727No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37120.13208676449342663No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT33000.11742627231366053No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG93850.027.7589931
TACATGG95200.027.165972
ACATGGG96300.025.7223033
GTATCAA160250.023.5119191
CATGGGA53350.021.0819364
GAGTACT68450.021.00483712-13
TATCAAC181450.020.791022
CATGGGG47500.020.6810844
TCAACGC182150.020.5823274
CAACGCA183350.020.5256355
ATCAACG183300.020.4258523
AACGCAG189500.019.781556
GTGTAGC17150.019.0919631
AGTACTT75400.018.91138312-13
GGTATCA121300.018.191051
GTACTTT79850.018.03734414-15
ACGCAGA203450.018.0290137
CGCAGAG202350.017.8224878
GTAGCCC15600.017.3386753
TAGCCCT19250.017.2569434
Produced by FastQC (version 0.11.5)