##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939595_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2810274 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.02197508143334 31.0 30.0 33.0 26.0 34.0 2 30.16687162888743 31.0 30.0 34.0 25.0 34.0 3 30.20655565969724 31.0 30.0 34.0 26.0 34.0 4 33.784525281164754 35.0 35.0 37.0 28.0 37.0 5 33.77560657786394 35.0 35.0 37.0 28.0 37.0 6 33.87399627224961 35.0 35.0 37.0 28.0 37.0 7 33.67522206019769 35.0 33.0 37.0 28.0 37.0 8 33.67281197491775 35.0 33.0 37.0 28.0 37.0 9 34.92472015184285 37.0 34.0 39.0 28.0 39.0 10-11 34.80917892703701 37.0 34.0 39.0 27.0 39.0 12-13 34.64230961109131 37.0 34.0 39.0 27.0 39.0 14-15 35.31624104980511 38.0 33.5 40.0 27.0 41.0 16-17 35.036301264574206 38.0 33.0 40.0 25.5 41.0 18-19 35.004114901251626 38.0 33.0 40.0 25.5 41.0 20-21 35.12256972095959 38.0 34.0 40.0 25.5 41.0 22-23 34.977084618795175 38.0 34.0 40.0 25.0 41.0 24-25 34.849477666590516 38.0 33.0 40.0 25.0 41.0 26-27 34.96929018309247 38.0 34.0 40.0 25.0 41.0 28-29 34.84921897295423 38.0 34.0 40.0 24.5 41.0 30-31 34.624173835006836 38.0 33.0 40.0 24.0 41.0 32-33 34.40718769771204 38.0 33.0 40.0 24.0 41.0 34-35 34.18054271576366 38.0 33.0 40.0 23.5 41.0 36-37 33.88596841446777 38.0 32.5 40.0 21.5 41.0 38-39 33.726389312928205 38.0 32.5 40.0 21.0 41.0 40-41 33.45352019055793 38.0 31.5 40.0 18.5 41.0 42-43 33.142634846281894 37.0 31.0 40.0 17.5 41.0 44-45 32.89508265030385 37.0 31.0 40.0 16.0 41.0 46-47 32.469080950825436 37.0 30.5 40.0 14.5 41.0 48-49 32.12043149529192 37.0 30.0 39.5 11.0 41.0 50-51 31.52116021427092 35.5 30.0 38.5 9.0 40.5 52-53 31.571836945436637 36.0 30.0 39.0 8.5 40.0 54-55 31.597064378775876 36.0 30.0 39.0 8.0 40.0 56-57 31.451673395547907 36.0 30.0 39.0 7.5 40.0 58-59 31.04479314116702 36.0 29.0 39.0 4.5 40.0 60-61 30.61364959431002 35.0 28.0 39.0 2.0 40.0 62-63 30.187668355469967 35.0 28.0 38.0 2.0 40.0 64-65 29.562228095908086 34.0 27.0 38.0 2.0 40.0 66-67 29.007173143971016 34.0 26.0 38.0 2.0 40.0 68-69 28.47711415328185 34.0 26.0 37.0 2.0 39.0 70-71 27.908747510029272 33.0 25.5 36.0 2.0 39.0 72-73 27.154172511292494 32.5 23.5 36.0 2.0 38.5 74-75 26.691060551391075 32.0 23.0 35.0 2.0 38.0 76-77 26.28652010444533 32.0 22.0 35.0 2.0 37.5 78-79 25.69153096815471 31.5 20.5 35.0 2.0 37.0 80-81 25.004690645823146 31.0 18.5 34.5 2.0 36.0 82-83 24.32658007724514 30.5 15.0 34.0 2.0 36.0 84-85 23.65368394683223 30.0 7.5 34.0 2.0 35.0 86-87 22.96512742173895 29.5 4.5 34.0 2.0 35.0 88-89 22.151184902255082 29.0 2.0 34.0 2.0 35.0 90-91 20.90530763192486 28.0 2.0 33.5 2.0 35.0 92-93 19.280584028461284 25.0 2.0 32.0 2.0 34.0 94-95 18.98145340988103 25.0 2.0 32.0 2.0 34.0 96-97 18.32067157864322 25.0 2.0 32.0 2.0 34.0 98-99 17.313121958926423 22.0 2.0 32.0 2.0 34.0 100-101 15.674515367540675 10.0 2.0 30.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.1072143884794343 1101 2 1.0947771058576947 1101 3 1.180489574119047 1101 4 1.2925082320980081 1101 5 1.0695074946943137 1101 6 1.1827761439645599 1101 7 1.1204801636109778 1101 8 1.1586498164747283 1101 9 1.3049770950748396 1101 10-11 1.2336366389908946 1101 12-13 1.1024747246203646 1101 14-15 1.3374540395295753 1101 16-17 1.0968650745818493 1101 18-19 1.2782095086500007 1101 20-21 1.3017193232467577 1101 22-23 1.3161195174986489 1101 24-25 1.4531997635895166 1101 26-27 1.1033866802786179 1101 28-29 1.3498383836877323 1101 30-31 1.600474962927251 1101 32-33 1.4560866935000476 1101 34-35 1.4955966653377644 1101 36-37 1.428330367383957 1101 38-39 1.0341092829459981 1101 40-41 1.3029161984181528 1101 42-43 1.3025983838668296 1101 44-45 1.3066197045143824 1101 46-47 1.3397021985080926 1101 48-49 1.1712835995170607 1101 50-51 1.2849054433742246 1101 52-53 1.2781811413315758 1101 54-55 1.1641064507749554 1101 56-57 1.2221828739907288 1101 58-59 1.2272569266706803 1101 60-61 1.0577063708864713 1101 62-63 1.1730302990681487 1101 64-65 1.128221094646939 1101 66-67 0.9143795112101536 1101 68-69 0.953862686349062 1101 70-71 0.7972905123154952 1101 72-73 1.1320062378571976 1101 74-75 1.153343042277161 1101 76-77 0.1205374007527702 1101 78-79 0.46945954498312403 1101 80-81 0.4071354614086147 1101 82-83 0.4749234429157134 1101 84-85 0.12985976084265616 1101 86-87 -0.6280020517352689 1101 88-89 -1.2560069231740023 1101 90-91 -4.136141208037554 1101 92-93 -9.354672199177944 1101 94-95 -7.495966713479287 1101 96-97 -4.301791289621235 1101 98-99 -2.932911440619389 1101 100-101 -2.4125737145781887 1102 1 1.1367477899293448 1102 2 1.0448330587599486 1102 3 1.0772929604860018 1102 4 1.1565670069727219 1102 5 1.0867799123462376 1102 6 1.055617091758073 1102 7 1.1306489954116827 1102 8 1.0504417689877457 1102 9 1.317043460082381 1102 10-11 1.1096279784547178 1102 12-13 1.1205012900852651 1102 14-15 1.193776717484134 1102 16-17 1.232818463381534 1102 18-19 1.2407575551638956 1102 20-21 1.4206678952313752 1102 22-23 1.37079523393907 1102 24-25 1.3086466569870723 1102 26-27 1.273240229638816 1102 28-29 1.4637147034464064 1102 30-31 1.3640252962119277 1102 32-33 1.4493358542065167 1102 34-35 1.4781053015465844 1102 36-37 1.5184753583828368 1102 38-39 1.490453855514481 1102 40-41 1.5429906401054936 1102 42-43 1.3759818315386951 1102 44-45 1.202790579812394 1102 46-47 1.1302072342692995 1102 48-49 1.2669752260436056 1102 50-51 1.207070090870939 1102 52-53 1.3316769813549278 1102 54-55 1.2689086694291625 1102 56-57 1.2494781593505273 1102 58-59 1.1450426513824006 1102 60-61 1.1618030964253236 1102 62-63 1.0469294865346974 1102 64-65 0.9795809934451647 1102 66-67 1.1557189750353878 1102 68-69 0.8535245362006663 1102 70-71 0.9283541040052725 1102 72-73 0.994118824827467 1102 74-75 0.8111180629554084 1102 76-77 0.7085050940335584 1102 78-79 0.7619350959975719 1102 80-81 0.6931277739663884 1102 82-83 0.6701990717776809 1102 84-85 0.18344074658682885 1102 86-87 -0.2093648698153565 1102 88-89 -0.8499226532377442 1102 90-91 -5.064132255573194 1102 92-93 -10.466942913915451 1102 94-95 -8.98276912675229 1102 96-97 -5.595325080308726 1102 98-99 -4.11646128735709 1102 100-101 -3.3735404834197205 1103 1 0.9063630160355238 1103 2 0.9869607710686203 1103 3 0.8803040590124418 1103 4 0.8791663850402358 1103 5 0.9319829914596269 1103 6 0.7401759894562261 1103 7 0.7970127653946548 1103 8 0.613641118811799 1103 9 0.913183905003315 1103 10-11 0.8081905881502891 1103 12-13 0.8516509429500942 1103 14-15 0.8750158560010064 1103 16-17 1.0306372326007747 1103 18-19 0.9178009549846351 1103 20-21 1.0092156050615415 1103 22-23 1.086112843159114 1103 24-25 0.8519912877038536 1103 26-27 0.9211866708189262 1103 28-29 0.8727203319642314 1103 30-31 0.8635311797854754 1103 32-33 1.0193278999263313 1103 34-35 1.034633142968211 1103 36-37 0.9401210874243091 1103 38-39 0.8994444977073286 1103 40-41 0.7663040300986736 1103 42-43 0.8132923049624736 1103 44-45 0.898221335786026 1103 46-47 0.910170085475265 1103 48-49 0.83669002475132 1103 50-51 0.6781480687545844 1103 52-53 0.838367654961413 1103 54-55 0.9654707488393974 1103 56-57 0.53796505928511 1103 58-59 0.47954722365251357 1103 60-61 0.4713385779907 1103 62-63 0.5689455507222796 1103 64-65 0.5696871369738119 1103 66-67 0.6642368274950208 1103 68-69 0.7107146579069159 1103 70-71 0.8028940670582152 1103 72-73 0.8149178265076671 1103 74-75 0.6172669232742116 1103 76-77 0.5330885750686498 1103 78-79 0.4809654231317566 1103 80-81 0.3354233619346765 1103 82-83 0.32435691236353037 1103 84-85 0.08764167285699287 1103 86-87 -0.22086024566950613 1103 88-89 -0.76529660527574 1103 90-91 -2.2182366353266687 1103 92-93 -8.770054449639105 1103 94-95 -5.420042422562171 1103 96-97 -2.4825862717791995 1103 98-99 -1.7292967865025801 1103 100-101 -1.3582277735751465 1104 1 0.9628039956560066 1104 2 0.8926050361443636 1104 3 0.9239700321572712 1104 4 0.9456684447165316 1104 5 0.9685198943414974 1104 6 0.9107163515916099 1104 7 1.1143516469026906 1104 8 1.0652633750138065 1104 9 1.0600534476966885 1104 10-11 0.8615976186234775 1104 12-13 0.9262734748897046 1104 14-15 1.2039900285793195 1104 16-17 1.2007514548118579 1104 18-19 0.9532946506204567 1104 20-21 1.0775471419226434 1104 22-23 1.0635575076365953 1104 24-25 1.1759407692336126 1104 26-27 0.9658732908608698 1104 28-29 1.1324171041631956 1104 30-31 1.2154003464730394 1104 32-33 1.2548358002262958 1104 34-35 1.1246063212200355 1104 36-37 0.9423921625502771 1104 38-39 0.9266556207949108 1104 40-41 0.9011848362551333 1104 42-43 0.9273962831040024 1104 44-45 0.9202243605439264 1104 46-47 0.9028417321300637 1104 48-49 0.7083065990876705 1104 50-51 0.9313721097308694 1104 52-53 1.0253749828969738 1104 54-55 1.0709583448253177 1104 56-57 1.0817831058931198 1104 58-59 0.8990301738777013 1104 60-61 0.9316102213032309 1104 62-63 0.9883040012440922 1104 64-65 1.0607306784472499 1104 66-67 1.0048884873518027 1104 68-69 0.9110572409833146 1104 70-71 1.0195757739575058 1104 72-73 0.9898261228670968 1104 74-75 0.7104424177218611 1104 76-77 0.5495839501971176 1104 78-79 0.4443265808954422 1104 80-81 0.4648909376998489 1104 82-83 0.5142778675854167 1104 84-85 0.3649915003121045 1104 86-87 0.325184590935077 1104 88-89 0.17759939151568105 1104 90-91 -0.4584370882047857 1104 92-93 -3.7110996622495023 1104 94-95 -1.3859432907178224 1104 96-97 -0.5225419030929643 1104 98-99 -0.3091102876180649 1104 100-101 -0.29209879522472626 1105 1 1.0381921081069017 1105 2 0.9683270087680711 1105 3 1.0213575464320641 1105 4 0.9357187185141385 1105 5 0.7744055361195734 1105 6 0.691312981467938 1105 7 0.8352100092934478 1105 8 0.8033946398957639 1105 9 1.032022934740965 1105 10-11 0.876389691487681 1105 12-13 0.8516974289149815 1105 14-15 1.115249002863841 1105 16-17 1.2173808937989108 1105 18-19 1.0522491210069802 1105 20-21 0.9692970436107444 1105 22-23 0.9275350584675266 1105 24-25 1.0378050473063993 1105 26-27 0.9345418758493764 1105 28-29 1.0760071023624604 1105 30-31 1.287555308735726 1105 32-33 1.2617213376880088 1105 34-35 1.1878659531875897 1105 36-37 1.2877404591285213 1105 38-39 1.2829550448562443 1105 40-41 1.0909685715769157 1105 42-43 1.1584464621445818 1105 44-45 1.129678661670269 1105 46-47 1.1033584028534804 1105 48-49 0.9882582120660643 1105 50-51 1.0427403473446724 1105 52-53 0.9562315732238993 1105 54-55 1.276040320402867 1105 56-57 1.2964787966473033 1105 58-59 1.1093856226394259 1105 60-61 1.05081724245197 1105 62-63 1.0478019109137655 1105 64-65 1.190370292283113 1105 66-67 1.1115999491156714 1105 68-69 1.1167787368346147 1105 70-71 1.1012078722352356 1105 72-73 1.3161694231334522 1105 74-75 1.2104316634571681 1105 76-77 0.851223509544706 1105 78-79 0.672329120813778 1105 80-81 0.8548296419758543 1105 82-83 0.7800907890256319 1105 84-85 0.7145306740954034 1105 86-87 0.5038700445852129 1105 88-89 0.40653599906854154 1105 90-91 -0.7188563874258271 1105 92-93 -7.085678169755919 1105 94-95 -2.6610153950752924 1105 96-97 -0.6174859289376116 1105 98-99 -0.11344343179583305 1105 100-101 0.010584883732750683 1106 1 0.8638597360865177 1106 2 1.0083260301917392 1106 3 0.9265100369517469 1106 4 0.9564911471546083 1106 5 0.9789324245072208 1106 6 1.0342715095746797 1106 7 1.135673860197329 1106 8 1.0871282691292166 1106 9 1.118867149108695 1106 10-11 0.8737621281971144 1106 12-13 1.006159407933957 1106 14-15 0.9850545008032512 1106 16-17 1.1505088644004147 1106 18-19 1.0928035989522513 1106 20-21 1.0535969595161276 1106 22-23 1.242995019387422 1106 24-25 1.1718596324086334 1106 26-27 1.0922981090515549 1106 28-29 1.0878706951167558 1106 30-31 1.1865474180341167 1106 32-33 1.163768422088495 1106 34-35 1.1159946004946661 1106 36-37 1.2873164809181574 1106 38-39 1.1808482587155211 1106 40-41 0.9708186405207613 1106 42-43 1.0137449236337375 1106 44-45 0.942804632199099 1106 46-47 1.1517248241798015 1106 48-49 1.0633606549874486 1106 50-51 0.903206736252077 1106 52-53 1.0007478546452795 1106 54-55 1.2052273037353096 1106 56-57 1.0340189733575862 1106 58-59 1.0174977342955565 1106 60-61 0.9383638714711076 1106 62-63 0.9671779511876188 1106 64-65 0.9734194188178975 1106 66-67 0.7360046842805836 1106 68-69 0.8960070521410692 1106 70-71 1.2451655353606164 1106 72-73 1.2081578444580643 1106 74-75 0.9859612034433134 1106 76-77 1.075488668822313 1106 78-79 1.036592929541591 1106 80-81 0.9791247165035948 1106 82-83 0.9409768927552982 1106 84-85 0.6511652098854022 1106 86-87 0.7329752223699266 1106 88-89 0.7557235088864971 1106 90-91 0.1595880510416059 1106 92-93 -3.425354532816895 1106 94-95 -1.1075771871633044 1106 96-97 -0.16431459767607492 1106 98-99 0.1285070625425888 1106 100-101 0.349962523492481 1107 1 0.4783527050232337 1107 2 0.522801558732624 1107 3 0.4846156223999962 1107 4 0.5521194212659566 1107 5 0.4000307818803037 1107 6 0.5647340676285921 1107 7 0.3373429706725446 1107 8 0.2400030277089229 1107 9 0.2404363971692618 1107 10-11 0.4384692264382153 1107 12-13 0.41249802290816007 1107 14-15 0.5757267957655614 1107 16-17 0.5300554787028346 1107 18-19 0.4991535178867039 1107 20-21 0.3990104583825058 1107 22-23 0.5419234039546126 1107 24-25 0.5783258206486366 1107 26-27 0.40546625384559576 1107 28-29 0.4843280339707263 1107 30-31 0.4508259346961552 1107 32-33 0.40669052811318096 1107 34-35 0.5461312815227402 1107 36-37 0.5633252786289376 1107 38-39 0.5280235605312598 1107 40-41 0.30813042958239834 1107 42-43 0.24534692088203514 1107 44-45 0.19777675986279064 1107 46-47 0.13464615507579225 1107 48-49 0.11663460484954413 1107 50-51 0.1049323665286046 1107 52-53 0.33440684969091805 1107 54-55 0.48417997566854254 1107 56-57 0.11578051793071964 1107 58-59 -0.04927637382197858 1107 60-61 -0.10730251313483663 1107 62-63 -0.22991789993599454 1107 64-65 0.12892154073272621 1107 66-67 -0.052993978622403404 1107 68-69 0.12113672471378223 1107 70-71 -0.0038558646510082895 1107 72-73 -0.03153940318910031 1107 74-75 0.12813434135808777 1107 76-77 0.011151371566914037 1107 78-79 -0.04706445065257725 1107 80-81 -0.09879186672907991 1107 82-83 -0.026554903670071184 1107 84-85 -0.0920717921751475 1107 86-87 -0.27516685872145175 1107 88-89 -0.33717243654444573 1107 90-91 -0.553325270191106 1107 92-93 -4.034969035474941 1107 94-95 -1.570921673737427 1107 96-97 -0.5579459464646135 1107 98-99 -0.515697558516365 1107 100-101 -0.47635858649182516 1108 1 0.37175930063991913 1108 2 0.5521328037102435 1108 3 0.2233558871435548 1108 4 -0.23509544845837382 1108 5 -0.8104815967446299 1108 6 -0.11083496386478942 1108 7 0.14859776735060137 1108 8 0.34726503962765776 1108 9 0.46561747092793127 1108 10-11 0.3545129469352375 1108 12-13 0.26545039892302924 1108 14-15 0.4373857625702726 1108 16-17 0.5634428384449777 1108 18-19 0.4665606323893172 1108 20-21 0.3395707009940736 1108 22-23 0.2521971218683774 1108 24-25 0.26629269507824205 1108 26-27 0.3265784976763939 1108 28-29 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0.611660419377003 2311 74-75 0.6384036926876995 2311 76-77 0.739264002350172 2311 78-79 0.7583731830203817 2311 80-81 0.7575094710576167 2311 82-83 0.8381089671688251 2311 84-85 0.8246977759236671 2311 86-87 1.0331758245202813 2311 88-89 1.0655341259596618 2311 90-91 1.917144162370377 2311 92-93 2.8756330154586998 2311 94-95 2.2197358550980653 2311 96-97 1.9651123462905034 2311 98-99 1.7501708911151823 2311 100-101 1.8443799611445737 2312 1 0.24970424686535964 2312 2 0.06578565029701622 2312 3 -0.06662354034522266 2312 4 -0.12428043260995025 2312 5 -0.029247571295428543 2312 6 0.054085673107522325 2312 7 0.17085487704203217 2312 8 -0.6862729694836176 2312 9 -0.23050831742787636 2312 10-11 -0.16540703124972111 2312 12-13 -0.12266874652137716 2312 14-15 -0.4081568009297456 2312 16-17 -0.06910236145529325 2312 18-19 -0.26018422635916494 2312 20-21 0.05803287242388677 2312 22-23 -0.13110266056501985 2312 24-25 -0.04559422615830755 2312 26-27 0.018224219352916293 2312 28-29 0.0905051120157978 2312 30-31 0.08106089555349882 2312 32-33 -0.005090109481663774 2312 34-35 -0.052146358855182484 2312 36-37 0.06249340783045909 2312 38-39 0.31108542239324777 2312 40-41 0.3392034499720964 2312 42-43 0.3278383695498235 2312 44-45 0.0759059930906929 2312 46-47 0.242086784507201 2312 48-49 0.29628715227344316 2312 50-51 0.10057302677247648 2312 52-53 0.04750873822788648 2312 54-55 0.0858611463145671 2312 56-57 -0.08973311909741355 2312 58-59 0.067071330677134 2312 60-61 0.1791687287701933 2312 62-63 0.13963764072481055 2312 64-65 0.25912222300236465 2312 66-67 0.27990720030952687 2312 68-69 -0.17643230563736623 2312 70-71 -0.02818677340752984 2312 72-73 0.12849671671094853 2312 74-75 0.2922947166009209 2312 76-77 0.4596045215941764 2312 78-79 0.5286640021388109 2312 80-81 0.6578478191719057 2312 82-83 0.8124613128901323 2312 84-85 0.7200924266431734 2312 86-87 0.665198020716872 2312 88-89 0.7382055764586752 2312 90-91 1.1757538298095405 2312 92-93 2.4002454178209476 2312 94-95 1.6437223685510851 2312 96-97 1.1488051639812156 2312 98-99 0.9945338071895868 2312 100-101 1.1007079339778496 2313 1 -0.41881352249962944 2313 2 -1.0148822363936247 2313 3 -0.4892520678736858 2313 4 -0.22972495631685064 2313 5 -1.0597485468460732 2313 6 -0.6558054573482792 2313 7 -1.5413847157762461 2313 8 -1.358055296468173 2313 9 -1.5483102674621563 2313 10-11 -2.156529351050388 2313 12-13 -0.6253151014338556 2313 14-15 -0.25592632084308775 2313 16-17 -1.7280907497504003 2313 18-19 -1.3953998965640508 2313 20-21 -1.579390957728421 2313 22-23 -1.3597454268136033 2313 24-25 -0.40434406443861803 2313 26-27 -0.9581706046729721 2313 28-29 -2.5969799023012 2313 30-31 -0.8816828882287311 2313 32-33 -0.48141121702841616 2313 34-35 -0.16623284444435882 2313 36-37 -4.840628335145425E-4 2313 38-39 -0.4847520434917172 2313 40-41 -0.3873652515806114 2313 42-43 -0.1785530082178468 2313 44-45 -0.9821642787717835 2313 46-47 -0.8171165768411299 2313 48-49 -0.34070330500777857 2313 50-51 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-1.515788040011806 2314 9 -1.8182350545064807 2314 10-11 -1.1169402407442064 2314 12-13 -2.2164012195091303 2314 14-15 -3.016064305621626 2314 16-17 -2.0935377443706713 2314 18-19 -2.5284823251716446 2314 20-21 -2.0413391940004075 2314 22-23 -3.2686831038151283 2314 24-25 -1.8769623926375445 2314 26-27 -1.9476208727573265 2314 28-29 -1.3167401545946547 2314 30-31 -1.5842573001518687 2314 32-33 -2.199680225705009 2314 34-35 -0.9291214683053184 2314 36-37 -0.5925184967711985 2314 38-39 -0.6274798885316173 2314 40-41 -0.7841503110621488 2314 42-43 -0.6314635256007648 2314 44-45 -1.1776190396128001 2314 46-47 -1.5306746731365237 2314 48-49 -1.9787686996224565 2314 50-51 -1.044441164116396 2314 52-53 -0.8226001673604841 2314 54-55 -0.42874763751524014 2314 56-57 -0.5183491036102161 2314 58-59 -0.6259905320534465 2314 60-61 -0.5369326783671795 2314 62-63 -0.8200891856841928 2314 64-65 -1.0246758731587526 2314 66-67 -0.9360290577582511 2314 68-69 -0.7744021091064006 2314 70-71 -0.9877730268048417 2314 72-73 -0.6002207230715868 2314 74-75 -0.2655094972650325 2314 76-77 -0.3870719795746602 2314 78-79 -0.5595419170812193 2314 80-81 -0.5246878426256458 2314 82-83 -0.5314673096717932 2314 84-85 -0.6191144825534955 2314 86-87 -0.3934396886232143 2314 88-89 -0.07146391941193642 2314 90-91 0.5584267606176354 2314 92-93 1.443708839061106 2314 94-95 0.7181346948411864 2314 96-97 0.7915641191499958 2314 98-99 0.8269813316973185 2314 100-101 1.1239345521532034 2315 1 -7.445166063775861 2315 2 -3.194413053169761 2315 3 -1.732513567920389 2315 4 -1.111855030838477 2315 5 -1.4835877204784964 2315 6 -1.8019541861975554 2315 7 -3.101404126483576 2315 8 -3.0501688992275895 2315 9 -3.3894238314912926 2315 10-11 -2.2318614632406266 2315 12-13 -2.8494472582778414 2315 14-15 -4.713758723510466 2315 16-17 -4.372159434492723 2315 18-19 -3.24648992624968 2315 20-21 -4.640301734544018 2315 22-23 -5.7400141694235245 2315 24-25 -3.859260304502591 2315 26-27 -4.673981445963101 2315 28-29 -3.0541521814417507 2315 30-31 -2.7725751200727657 2315 32-33 -1.970360555537944 2315 34-35 -2.764140156038607 2315 36-37 -3.528602249436023 2315 38-39 -2.601709529030515 2315 40-41 -3.950778688255454 2315 42-43 -7.873492213062317 2315 44-45 -4.387932926834242 2315 46-47 -3.134483919920825 2315 48-49 -5.983766711040133 2315 50-51 -7.0524415025459355 2315 52-53 -7.203860805040787 2315 54-55 -4.9517318202573115 2315 56-57 -4.183597515832215 2315 58-59 -4.692785900756373 2315 60-61 -3.5767762720442313 2315 62-63 -3.6730418598951253 2315 64-65 -2.3795581515553366 2315 66-67 -2.825328632127526 2315 68-69 -2.377624662347298 2315 70-71 -2.1559107503350816 2315 72-73 -2.3792571128930113 2315 74-75 -3.0183947943542933 2315 76-77 -3.0692693506935314 2315 78-79 -2.159257586862921 2315 80-81 -1.9292414491234915 2315 82-83 -2.3357176264863035 2315 84-85 -2.277172193435856 2315 86-87 -2.7493906756270228 2315 88-89 -2.090401460634876 2315 90-91 -1.0745417728461284 2315 92-93 -0.21313163711172578 2315 94-95 -0.5318813620347349 2315 96-97 -0.579599213862199 2315 98-99 -0.6271759787512607 2315 100-101 -0.4729952496555434 2316 1 -4.434754884734296 2316 2 -3.133346923034882 2316 3 -4.927517277635957 2316 4 -4.478576452258924 2316 5 -4.320716627424577 2316 6 -4.37245627756996 2316 7 -2.9962672233994923 2316 8 -1.6299864265806647 2316 9 -1.9012016114290233 2316 10-11 -3.1354258681147726 2316 12-13 -4.194114177658754 2316 14-15 -3.254696001778015 2316 16-17 -3.105746778044786 2316 18-19 -3.28591010904632 2316 20-21 -5.94311836443752 2316 22-23 -6.829868340342593 2316 24-25 -4.697726815004813 2316 26-27 -4.288671271622668 2316 28-29 -4.754768739408895 2316 30-31 -6.298710633228488 2316 32-33 -4.674231943425156 2316 34-35 -6.333164031055212 2316 36-37 -9.520218940240142 2316 38-39 -6.264748126272039 2316 40-41 -5.361727533059749 2316 42-43 -4.731325382779374 2316 44-45 -3.457126249994211 2316 46-47 -5.774269242406888 2316 48-49 -3.3349223927368143 2316 50-51 -4.7069884150582055 2316 52-53 -5.591205005086792 2316 54-55 -7.325633728249887 2316 56-57 -5.1253818728202525 2316 58-59 -6.069014399795737 2316 60-61 -6.524977788977704 2316 62-63 -6.638015503095001 2316 64-65 -5.262846611909982 2316 66-67 -6.665116384558278 2316 68-69 -5.302446385404881 2316 70-71 -6.063722698535141 2316 72-73 -8.405144447697271 2316 74-75 -8.11834230754889 2316 76-77 -7.275585748932304 2316 78-79 -6.3515379765612465 2316 80-81 -6.275670726393141 2316 82-83 -5.201297783881923 2316 84-85 -6.574104631253572 2316 86-87 -5.4045230428143505 2316 88-89 -5.421027349589643 2316 90-91 -6.126462725125254 2316 92-93 -4.344011050296281 2316 94-95 -4.417307201742627 2316 96-97 -5.060465505345405 2316 98-99 -5.850171193380174 2316 100-101 -4.853356436634927 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 16002.0 3 6405.0 4 7060.0 5 7902.0 6 8872.0 7 10456.0 8 12570.0 9 15532.0 10 18491.0 11 21730.0 12 24924.0 13 27579.0 14 29979.0 15 32914.0 16 36249.0 17 39537.0 18 38259.0 19 38152.0 20 40468.0 21 44456.0 22 48748.0 23 53998.0 24 60096.0 25 67897.0 26 75885.0 27 85610.0 28 95867.0 29 108308.0 30 123993.0 31 140459.0 32 162720.0 33 187105.0 34 218807.0 35 261930.0 36 299731.0 37 260347.0 38 79970.0 39 1266.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.305956857735374 18.91111172050873 13.397009052759865 23.385922368996034 2 15.255659587381452 20.433390879906938 40.52481477827354 23.786134754438073 3 19.147157607553595 25.45982968083856 29.780814696883894 25.61219801472395 4 12.452116627600127 16.59896359991067 38.41030943477408 32.53861033771513 5 13.377996701891604 37.90060797743324 34.843304234472 13.87809108620315 6 31.099973612342158 37.00105158911746 18.03786931592579 13.861105482614594 7 27.658132430891353 32.9327453024426 21.91735205173893 17.49177021492712 8 25.542773338309782 35.325906177369944 20.307176837872085 18.824143646448192 9 26.424988372747286 16.635945553831153 20.558585328365726 36.380480745055834 10-11 24.51731119551688 26.47269366582795 28.372001502921258 20.637993635733913 12-13 25.880442987360087 23.921445654664954 28.280447545942405 21.917663812032558 14-15 22.871583497180396 25.306308377471577 26.58229426012034 25.239813865227696 16-17 22.20406654356974 28.45399865268877 27.018878918105166 22.323055885636318 18-19 22.077131360870876 27.550318449786616 28.829851740321626 21.54269844902088 20-21 23.014403447922742 26.74620991188741 28.55359106674562 21.685795573444228 22-23 22.520117839420635 26.677947143248993 28.471596353675 22.330338663655372 24-25 22.447633975321242 26.77660737783102 28.636315210563318 22.139443436284427 26-27 22.492656009030863 26.804622759031577 28.64494992830611 22.057771303631448 28-29 22.161056583066234 27.149021613060125 28.790320712798046 21.899601091075592 30-31 22.4582297145663 26.88349788213277 28.608240351116628 22.050032052184307 32-33 22.259331005017525 26.992529922445097 28.549578499773826 22.19856057276355 34-35 22.664531723432212 26.92176958005182 28.382434889932135 22.031263806583834 36-37 22.713018449353537 26.73983981437338 28.527176612527942 22.01996512374514 38-39 22.38569330795847 26.98682035525879 28.59049254920655 22.036993787576186 40-41 22.565929334541337 26.908139464043256 28.560839608797252 21.96509159261815 42-43 22.30511700354634 27.037915975200566 28.307422801030913 22.349544220222185 44-45 22.481461320991315 27.08547670481749 28.114277205262077 22.318784768929117 46-47 22.61013312391905 27.156395841454195 27.742808094470167 22.490662940156593 48-49 22.57367222497425 27.10478579905275 27.91342511252633 22.408116863446672 50-51 22.478526575295497 27.25752302738444 27.934511289464027 22.329439107856032 52-53 22.559567335822134 27.363984656575663 27.756213995964714 22.320234011637492 54-55 22.40499552507083 27.147208595855975 27.806864124327113 22.64093175474608 56-57 22.472999487435214 27.45777665180995 27.568823447353612 22.500400413401227 58-59 22.597787203174384 27.20485606060741 27.69284568693095 22.504511049287252 60-61 22.50427795312368 27.194385985913662 27.883614735896295 22.417721325066363 62-63 22.45979645773834 27.14531559417735 27.946474010535688 22.44841393754862 64-65 22.337463643827956 27.278839594698812 27.82580740534928 22.557889356123955 66-67 22.347717334244415 27.269061017450934 27.607794504059868 22.77542714424478 68-69 22.20561898983868 27.356941550407427 27.81164132600551 22.625798133748386 70-71 22.447975453477902 27.131431633432523 27.56362158110157 22.856971331988003 72-73 22.3562817459575 27.059579271677237 27.648716530038215 22.93542245232705 74-75 22.20972714797455 27.584805118425976 27.365240327208568 22.840227406390902 76-77 22.327256887820482 27.16337251102597 27.501253821616206 23.008116779537346 78-79 22.408090951199686 27.19478796059675 27.529184144955032 22.86793694324853 80-81 22.358327753200637 27.41766192647984 27.293006782245072 22.931003538074446 82-83 22.399070633891707 27.29390918005684 27.43496094787358 22.87205923817787 84-85 22.42693465904029 27.17797259492881 27.201849575025395 23.193243171005506 86-87 22.163103190824 27.16931306496936 27.514909934551312 23.15267380965533 88-89 22.467515987591327 27.072701524208 27.317599236060293 23.142183252140384 90-91 22.043381616194157 27.06760331884687 27.673426838006872 23.2155882269521 92-93 21.86766447144845 27.10250870157791 27.966567339302518 23.063259487671125 94-95 22.25603376893077 27.129459093276264 27.260277341815964 23.354229795976998 96-97 22.137741893394004 26.98172372906371 27.277867338215465 23.602667039326818 98-99 22.218995285106132 26.974894429236834 27.060916371696088 23.745193913960943 100-101 22.428966562190038 26.916065015259626 27.170656587367485 23.484311835182847 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2594.0 1 1569.5 2 560.0 3 586.5 4 542.5 5 518.5 6 750.0 7 1336.0 8 2068.5 9 2375.5 10 2081.5 11 1799.0 12 1816.5 13 1927.0 14 2046.0 15 2234.5 16 2489.5 17 2701.0 18 2974.0 19 3353.0 20 3935.0 21 4694.5 22 5659.0 23 7006.5 24 8742.0 25 11204.5 26 14754.0 27 19098.0 28 23561.5 29 28235.0 30 33220.0 31 38651.0 32 44597.0 33 50420.5 34 56414.0 35 62257.0 36 68744.5 37 74927.5 38 79047.0 39 81703.5 40 84431.0 41 87787.0 42 90336.0 43 93468.5 44 97013.0 45 99635.0 46 102256.5 47 107266.0 48 116922.5 49 116963.5 50 107837.0 51 102247.5 52 98315.5 53 95029.0 54 91092.0 55 85631.5 56 78520.0 57 70777.0 58 63682.5 59 57094.0 60 48715.5 61 39681.5 62 31714.0 63 24704.0 64 19105.0 65 14540.0 66 10427.0 67 7392.0 68 5142.5 69 3352.5 70 2299.5 71 1592.5 72 1098.0 73 724.5 74 491.0 75 353.5 76 219.5 77 141.0 78 97.5 79 61.0 80 42.0 81 28.0 82 21.0 83 20.0 84 18.5 85 14.5 86 13.5 87 12.0 88 7.5 89 8.0 90 8.0 91 5.5 92 3.5 93 2.5 94 2.0 95 2.0 96 3.0 97 3.0 98 2.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08543650903790877 2 0.09582695495172357 3 0.09266000397114303 4 0.09504411313629917 5 0.09266000397114303 6 0.07625590956611349 7 0.08842554142407466 8 0.08468925094136728 9 0.07885352104456719 10-11 0.08572117878897217 12-13 0.08481379395745753 14-15 0.08979551460106737 16-17 0.08751815659256001 18-19 0.09008018435213079 20-21 0.09915403266727728 22-23 0.10902851465728965 24-25 0.13276285515220224 26-27 0.13870533620565112 28-29 0.13767340835804623 30-31 0.13923909198889503 32-33 0.13559176080339497 34-35 0.1295959041716217 36-37 0.1345064573774657 38-39 0.12666024736378018 40-41 0.12251474411391913 42-43 0.12226565808173866 44-45 0.11280038885887995 46-47 0.13222909936895832 48-49 0.15172897731680257 50-51 0.16149670815016615 52-53 0.16982329836877116 54-55 0.16521520677343207 56-57 0.13520033989568278 58-59 0.12648232876936555 60-61 0.12244357667615328 62-63 0.11879624549065322 64-65 0.10600389855224082 66-67 0.11911649896059956 68-69 0.11589617240169464 70-71 0.1253258579056704 72-73 0.153241285369327 74-75 0.16809748800294919 76-77 0.17261662030108096 78-79 0.16971654721212237 80-81 0.1755878608278054 82-83 0.1751074806228859 84-85 0.15030562856148547 86-87 0.15155105872238792 88-89 0.17140677385906145 90-91 0.26020594433140687 92-93 0.5267813743428577 94-95 0.1727589551766127 96-97 0.18560467769334948 98-99 0.20485546960901324 100-101 0.21273726334158166 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2810274.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.02831189354755 #Duplication Level Percentage of deduplicated Percentage of total 1 75.26192689932246 42.920606409239376 2 13.015151547655224 14.844642436029403 3 4.856634784867083 8.308970497933567 4 2.3347427143640265 5.325857428237581 5 1.3324458535594468 3.799356885902616 6 0.8282048781323307 2.8338675661132857 7 0.5195121060139118 2.0738828889964576 8 0.35050209724106623 1.5990834336644844 9 0.25434795313483544 1.3054530960772928 >10 1.173070017150467 11.1428324954415 >50 0.04955701116416816 1.9304239548960827 >100 0.02188336239828114 2.2224150391978412 >500 0.001262984372984337 0.5249245167836248 >1k 6.314921864438114E-4 0.7082663605259562 >5k 1.2629843728876228E-4 0.4594169909609815 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7552 0.26872824500386794 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5205 0.18521325678563727 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3712 0.13208676449342663 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3300 0.11742627231366053 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.558371888292743E-5 2 0.0 0.0 0.0 0.0 3.558371888292743E-5 3 0.0 0.0 0.0 0.0 3.558371888292743E-5 4 0.0 0.0 0.0 0.0 3.558371888292743E-5 5 0.0 0.0 0.0 0.0 3.558371888292743E-5 6 0.0 0.0 0.0 0.0 7.116743776585486E-5 7 0.0 0.0 0.0 0.0 1.0675115664878229E-4 8 0.0 0.0 0.0 0.0 1.4233487553170972E-4 9 0.0 0.0 0.0 0.0 1.4233487553170972E-4 10-11 0.0 0.0 0.0 0.0 1.7791859441463714E-4 12-13 0.0 1.7791859441463715E-5 0.0 0.0 4.0921276715366546E-4 14-15 0.0 5.3375578324391146E-5 0.0 0.0 4.447964860365929E-4 16-17 0.0 7.116743776585486E-5 0.0 8.895929720731857E-5 4.981720643609841E-4 18-19 0.0 1.4233487553170972E-4 0.0 1.2454301609024601E-4 5.337557832439115E-4 20-21 0.0 2.1350231329756458E-4 0.0 1.9571045385610088E-4 5.871313615683026E-4 22-23 0.0 2.1350231329756458E-4 0.0 4.2700462659512917E-4 6.760906587756211E-4 24-25 0.0 2.1350231329756458E-4 0.0 8.71801112631722E-4 6.760906587756211E-4 26-27 0.0 2.1350231329756458E-4 0.0 0.001369973176992706 7.116743776585486E-4 28-29 0.0 2.1350231329756458E-4 0.0 0.0035583718882927426 7.47258096541476E-4 30-31 0.0 2.3129417273902832E-4 0.0 0.008967097158497712 7.47258096541476E-4 32-33 0.0 2.4908603218049203E-4 0.0 0.02126127203254914 7.47258096541476E-4 34-35 0.0 2.4908603218049203E-4 0.0 0.03933780122507627 7.47258096541476E-4 36-37 0.0 3.0246161050488315E-4 0.0 0.06798269492583285 7.47258096541476E-4 38-39 0.0 3.914209077122017E-4 0.0 0.10972239717550673 7.650499559829398E-4 40-41 0.0 4.2700462659512917E-4 0.0 0.1563192770527002 7.828418154244035E-4 42-43 0.0 4.6258834547805664E-4 0.0 0.20679478228813275 7.828418154244035E-4 44-45 0.0 5.159639238024478E-4 0.0 0.2665932218708923 8.006336748658672E-4 46-47 0.0 5.337557832439115E-4 0.0 0.3368176910863496 8.71801112631722E-4 48-49 0.0 5.337557832439115E-4 0.0 0.41382085874900454 9.429685503975769E-4 50-51 0.0 5.337557832439115E-4 0.0 0.4956989958986206 9.607604098390406E-4 52-53 0.0 5.337557832439115E-4 0.0 0.5777194679237683 0.0010319278476048955 54-55 0.0 5.871313615683026E-4 0.0 0.6692052091717747 0.0011386790042536778 56-57 0.0 6.049232210097663E-4 0.0 0.7768637506520717 0.0011386790042536778 58-59 0.0 6.760906587756211E-4 0.0 0.8997343319548201 0.0011386790042536778 60-61 0.0 6.760906587756211E-4 0.0 1.0378881205177857 0.0011742627231366052 62-63 0.0 6.938825182170849E-4 0.0 1.1748854382170564 0.0011742627231366052 64-65 0.0 7.116743776585486E-4 0.0 1.3245327679792078 0.0011742627231366052 66-67 0.0 8.006336748658672E-4 0.0 1.4953346186172594 0.0011742627231366052 68-69 0.0 8.184255343073309E-4 0.0 1.6861345192675161 0.0011742627231366052 70-71 0.0 8.71801112631722E-4 0.0 1.8929470934150905 0.0011920545825780688 72-73 0.0 9.251766909561132E-4 1.7791859441463715E-5 2.104972682379014 0.0012810138797853875 74-75 0.0 9.251766909561132E-4 3.558371888292743E-5 2.3335802843423807 0.0013165975986683149 76-77 0.0 9.251766909561132E-4 3.558371888292743E-5 2.579695075996148 0.0013521813175512423 78-79 0.0 9.251766909561132E-4 3.558371888292743E-5 2.8586002646005335 0.0013877650364341697 80-81 0.0 9.251766909561132E-4 3.558371888292743E-5 3.163303649395041 0.001441140614758561 82-83 0.0 9.251766909561132E-4 3.558371888292743E-5 3.464448662301256 0.001494516193082952 84-85 0.0 9.251766909561132E-4 3.558371888292743E-5 3.7767491710772685 0.001494516193082952 86-87 1.7791859441463715E-5 9.607604098390406E-4 3.558371888292743E-5 4.119135714168796 0.0015123080525244158 88-89 3.558371888292743E-5 9.607604098390406E-4 3.558371888292743E-5 4.479954623641681 0.0015300999119658796 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 9385 0.0 27.758993 1 TACATGG 9520 0.0 27.16597 2 ACATGGG 9630 0.0 25.722303 3 GTATCAA 16025 0.0 23.511919 1 CATGGGA 5335 0.0 21.081936 4 GAGTACT 6845 0.0 21.004837 12-13 TATCAAC 18145 0.0 20.79102 2 CATGGGG 4750 0.0 20.681084 4 TCAACGC 18215 0.0 20.582327 4 CAACGCA 18335 0.0 20.525635 5 ATCAACG 18330 0.0 20.425852 3 AACGCAG 18950 0.0 19.78155 6 GTGTAGC 1715 0.0 19.091963 1 AGTACTT 7540 0.0 18.911383 12-13 GGTATCA 12130 0.0 18.19105 1 GTACTTT 7985 0.0 18.037344 14-15 ACGCAGA 20345 0.0 18.029013 7 CGCAGAG 20235 0.0 17.822487 8 GTAGCCC 1560 0.0 17.338675 3 TAGCCCT 1925 0.0 17.256943 4 >>END_MODULE