FastQCFastQC Report
Fri 27 May 2016
SRR939595_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939595_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2810274
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80880.28780111832511707No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61960.22047672219861836No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45730.16272434645162714No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT39140.13927467570777796No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG94150.024.457111
ACATGGG91400.023.6908783
TACATGG95150.023.5009442
GTATCAA151750.022.9004611
GAGTACT71800.020.71975912-13
TCAACGC170750.020.0696664
ATCAACG171300.020.005233
GGTATCA113650.019.9293081
CATGGGA54400.019.7290174
CAACGCA173550.019.6916245
AACGCAG174500.019.609726
TATCAAC176250.019.4447632
AGTACTT77300.019.3367312-13
CATGGGG41200.018.7376944
GTACTTT79900.018.47202114-15
CGCAGAG186400.017.7781438
ACGCAGA190350.017.6805427
AGAGTAC143450.016.89131710-11
GCAGAGT196150.016.3669519
ACTTTTT96700.015.45738616-17
Produced by FastQC (version 0.11.5)