FastQCFastQC Report
Fri 27 May 2016
SRR939594_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939594_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2809546
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32140.11439570663730012No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG82900.031.1230491
GTACCGG2302.4385263E-630.673751
ACATGGG93150.025.3116073
TACATGG96150.025.3067722
GTATCAA147450.024.6751121
GTATAGA8950.022.5218281
GTATAGG8050.021.2838291
GAGTACT69450.021.1697312-13
GGTATCA118550.020.8286551
TATCAAC178500.020.1959882
CATGGGG47550.020.1701354
CATGGGA53850.019.8283254
GTACTTT75950.019.14268114-15
TCAACGC189900.019.0575524
ATCAACG190500.019.048833
CAACGCA191650.018.9638655
AGTACTT75350.018.94835912-13
AACGCAG196650.018.5777026
ACGCAGA201950.017.5058387
ATCTAGG10400.017.443591
Produced by FastQC (version 0.11.5)