FastQCFastQC Report
Fri 27 May 2016
SRR939594_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939594_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2809546
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79030.2812909986168584No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61500.21889657617280514No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45410.1616275369757249No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT38580.13731755949181826No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA28180.10030090270812438No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG93700.022.6642251
GTATCAA155250.022.6460591
TACATGG93650.022.0680852
ACATGGG91600.020.9617563
GGTATCA117750.020.800531
TCAACGC174600.019.8593064
GAGTACT70550.019.81285712-13
ATCAACG176400.019.7104993
CAACGCA175900.019.6857155
AACGCAG178000.019.4396216
TATCAAC179350.019.3630922
AGTACTT75000.018.60586512-13
GTACCGT3306.6211214E-1018.5712496
GTACTTT78500.017.98649614-15
ACGCAGA191250.017.7230787
CGCAGAG189200.017.6410268
CATGGGG45800.017.3047284
CATGGGA48600.017.2784484
AGTACAT74500.017.1637172
AGAGTAC144400.016.27457410-11
Produced by FastQC (version 0.11.5)