##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939594_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2809546 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.51008276782085 31.0 31.0 34.0 30.0 34.0 2 31.724725275898667 31.0 31.0 34.0 30.0 34.0 3 31.78247802313968 31.0 31.0 34.0 30.0 34.0 4 35.57222732783161 37.0 35.0 37.0 33.0 37.0 5 35.329282738207525 37.0 35.0 37.0 33.0 37.0 6 35.328357321787934 37.0 35.0 37.0 32.0 37.0 7 35.23813847504187 37.0 35.0 37.0 32.0 37.0 8 35.19824733248717 37.0 35.0 37.0 32.0 37.0 9 36.81240776979626 39.0 37.0 39.0 32.0 39.0 10-11 36.69280303650483 39.0 35.0 39.0 32.0 39.0 12-13 36.62938656281122 39.0 35.0 39.0 32.0 39.0 14-15 37.72500094321289 39.5 37.0 41.0 32.0 41.0 16-17 37.67934196485838 39.0 36.0 41.0 32.0 41.0 18-19 37.64296989620387 39.0 36.5 41.0 32.0 41.0 20-21 37.57932295822884 39.0 36.0 41.0 32.0 41.0 22-23 37.45142631585317 39.0 36.0 41.0 32.0 41.0 24-25 37.32669833489112 39.0 36.0 41.0 31.0 41.0 26-27 37.04776074141516 39.0 36.0 40.0 31.0 41.0 28-29 36.85586283335457 39.0 36.0 40.0 30.5 41.0 30-31 36.628146148879566 38.5 35.0 40.0 30.0 41.0 32-33 36.48233308869119 38.0 35.0 40.0 30.0 41.0 34-35 36.465670254197654 38.0 35.0 40.0 30.0 41.0 36-37 36.39109752963646 38.0 35.0 40.0 30.0 41.0 38-39 36.437940329149264 38.5 35.0 40.0 30.0 41.0 40-41 36.33552004487558 39.0 35.0 40.0 30.0 41.0 42-43 36.17495477917073 38.0 35.0 40.0 30.0 41.0 44-45 35.98304245596975 38.0 35.0 40.0 29.0 41.0 46-47 35.72490857953563 38.0 35.0 40.0 28.0 41.0 48-49 35.454736103270776 38.0 34.0 40.0 27.0 41.0 50-51 35.23100209072925 38.0 34.0 40.0 27.0 41.0 52-53 35.00904790311317 38.0 34.0 40.0 26.5 41.0 54-55 34.73107042917255 38.0 33.5 40.0 26.0 41.0 56-57 33.92871108001079 37.5 32.5 40.0 23.0 41.0 58-59 30.80599890516119 35.5 27.5 39.0 8.5 40.5 60-61 30.530265032143983 34.5 26.5 39.0 10.5 40.0 62-63 32.387596964064656 35.5 30.5 39.0 20.5 40.0 64-65 32.447778573477706 36.0 31.0 39.0 19.5 40.0 66-67 27.497624171307393 32.0 19.0 37.0 4.5 39.5 68-69 19.91094219493114 20.5 8.0 32.5 2.0 37.0 70-71 17.955615960728174 16.0 7.5 29.0 2.0 35.0 72-73 19.70157758584483 20.0 7.5 31.0 2.0 35.5 74-75 21.158000438505013 24.0 9.5 32.5 2.0 36.0 76-77 23.01039473993307 27.5 12.5 33.0 2.0 35.5 78-79 25.217931117696594 30.5 14.0 34.5 2.0 36.5 80-81 26.852719620892486 32.5 23.0 35.0 2.0 37.0 82-83 28.028039405654866 33.5 27.0 35.0 2.0 36.0 84-85 28.08691920331612 34.0 28.0 35.0 2.0 36.0 86-87 27.814446355389805 34.0 27.5 35.0 2.0 36.0 88-89 27.37981901702268 33.0 26.5 35.0 2.0 35.5 90-91 25.522126172698364 32.5 16.0 34.5 2.0 35.0 92-93 23.89345609575355 31.5 4.5 34.0 2.0 35.0 94-95 20.06408918024478 28.5 2.0 34.0 2.0 35.0 96-97 17.33083565814548 18.5 2.0 33.0 2.0 35.0 98-99 17.611631736942552 22.0 2.0 33.0 2.0 35.0 100-101 16.83579517829571 18.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.2825027681812351 1101 2 0.2231104854587187 1101 3 0.2116610726543513 1101 4 0.17992713721768183 1101 5 0.3837616088411835 1101 6 0.37767535564977806 1101 7 0.3427196421693566 1101 8 0.3851651144124375 1101 9 0.38459967038277654 1101 10-11 0.34306273619647953 1101 12-13 0.31056200795185873 1101 14-15 0.4367155345159688 1101 16-17 0.3463171263248128 1101 18-19 0.3259423340259531 1101 20-21 0.42994825893781297 1101 22-23 0.4615293601565327 1101 24-25 0.4299650505304271 1101 26-27 0.5042735457739411 1101 28-29 0.38053217742846357 1101 30-31 0.25241981739900865 1101 32-33 0.43885935899523076 1101 34-35 0.227722003116952 1101 36-37 0.28100779884760385 1101 38-39 0.2656691639656046 1101 40-41 0.23216807956524121 1101 42-43 0.353672441257622 1101 44-45 0.31937455828425954 1101 46-47 0.11875164184665721 1101 48-49 0.03930276584333825 1101 50-51 0.08671869074817806 1101 52-53 0.02621160203252515 1101 54-55 0.23013980430568637 1101 56-57 -3.413985054504412 1101 58-59 -10.58742999659199 1101 60-61 -12.259712011243458 1101 62-63 -4.093621013414992 1101 64-65 -1.0953712631901205 1101 66-67 -2.01636024018498 1101 68-69 -4.714441279670767 1101 70-71 -4.929252333354393 1101 72-73 -3.786087539826191 1101 74-75 -6.591144673155295 1101 76-77 -5.118024733321931 1101 78-79 1.1430997812432864 1101 80-81 1.8664509242877294 1101 82-83 0.5170504967549263 1101 84-85 0.08533404169020642 1101 86-87 0.1917619153133252 1101 88-89 0.08556832576114459 1101 90-91 1.4912908840196017 1101 92-93 0.8978472891770934 1101 94-95 -8.829944953318792 1101 96-97 -9.680296530902728 1101 98-99 -8.881102805649522 1101 100-101 -9.751101019475787 1102 1 0.31192983335061086 1102 2 0.3984682427766124 1102 3 0.3334511379864722 1102 4 0.17194516748900668 1102 5 0.34834334374620823 1102 6 0.3486638818407499 1102 7 0.32685015529391137 1102 8 0.2612782017621811 1102 9 0.36514679786179727 1102 10-11 0.390492463475546 1102 12-13 0.39771921773540697 1102 14-15 0.49369514438321005 1102 16-17 0.4506719715969538 1102 18-19 0.35328562992700085 1102 20-21 0.4039411037914533 1102 22-23 0.3909115240215897 1102 24-25 0.37831256628221155 1102 26-27 0.14900977604060728 1102 28-29 0.31028779227118264 1102 30-31 0.20162249229613138 1102 32-33 0.09370710143321048 1102 34-35 0.22862438855323575 1102 36-37 0.005820201488788257 1102 38-39 0.18869975497720048 1102 40-41 0.11730647439446784 1102 42-43 0.2238153988731213 1102 44-45 0.11269051234252458 1102 46-47 0.11392293523671526 1102 48-49 0.12717127622440216 1102 50-51 -0.03077523522424741 1102 52-53 -0.10762552684148119 1102 54-55 -0.24743642619349515 1102 56-57 -3.8626856776008296 1102 58-59 -10.28966459936802 1102 60-61 -8.011226885345433 1102 62-63 -2.4457445980793793 1102 64-65 -0.8090324487008047 1102 66-67 -2.083238480635096 1102 68-69 -5.1253739480579235 1102 70-71 -4.832699997905321 1102 72-73 -3.6122301254438227 1102 74-75 -4.370431512568469 1102 76-77 -4.248942550856981 1102 78-79 1.7624863916452753 1102 80-81 2.0576570982897273 1102 82-83 0.7378479381071372 1102 84-85 0.4348747115188516 1102 86-87 0.18745342446214863 1102 88-89 0.24295902232713829 1102 90-91 1.607585988760249 1102 92-93 0.9816448932783679 1102 94-95 -9.262662746441482 1102 96-97 -10.011578063533916 1102 98-99 -9.146856043193406 1102 100-101 -10.137227533623332 1103 1 0.18761325985624566 1103 2 0.14781519854282266 1103 3 0.1644216385466244 1103 4 0.1594821500474879 1103 5 0.21092886809532985 1103 6 0.3195492841020311 1103 7 0.18649732981247524 1103 8 0.29502277835204893 1103 9 0.2811478483201171 1103 10-11 0.2997060445605868 1103 12-13 0.24960424563121109 1103 14-15 0.3274608139526052 1103 16-17 0.32429394349719587 1103 18-19 0.3319293373743619 1103 20-21 0.3211469684779047 1103 22-23 0.3710795833836613 1103 24-25 0.4277888736977218 1103 26-27 0.528276215294234 1103 28-29 0.32983826036769415 1103 30-31 0.26538230891292613 1103 32-33 0.2974614422566475 1103 34-35 0.2883852695972138 1103 36-37 0.271224711754499 1103 38-39 0.31221137287857914 1103 40-41 0.3727875060786374 1103 42-43 0.3670901607738912 1103 44-45 0.3181714043380879 1103 46-47 0.33650380795525336 1103 48-49 0.3477783489379078 1103 50-51 0.10232984692661518 1103 52-53 0.24605903952317476 1103 54-55 0.37584261017862985 1103 56-57 0.732440866307492 1103 58-59 -8.244558449996202 1103 60-61 -7.682414224528923 1103 62-63 -2.230830185288376 1103 64-65 -0.49903444624787596 1103 66-67 -1.6563762918263905 1103 68-69 -3.277633830328366 1103 70-71 -3.9727765891680775 1103 72-73 -2.909053397006854 1103 74-75 -3.6135626745821092 1103 76-77 -0.37651980564061915 1103 78-79 3.489659178069779 1103 80-81 2.8638587136487708 1103 82-83 1.2637909060430133 1103 84-85 0.7743906625607906 1103 86-87 0.6438953740680873 1103 88-89 0.476014794400033 1103 90-91 1.8582100476082637 1103 92-93 1.3090882565330055 1103 94-95 -7.681021391350251 1103 96-97 -9.32192493828073 1103 98-99 -8.545473195836735 1103 100-101 -9.666020847567886 1104 1 0.19237564953716912 1104 2 0.1920059656847073 1104 3 0.22775429299333183 1104 4 0.11907685473180152 1104 5 0.29557516816321794 1104 6 0.18361038389836182 1104 7 0.20186935968348507 1104 8 0.23732895622034533 1104 9 0.18044995286865628 1104 10-11 0.18759945936032096 1104 12-13 0.26666229043773626 1104 14-15 0.33348389609788853 1104 16-17 0.31868294553385823 1104 18-19 0.2930977012575866 1104 20-21 0.3232236826666295 1104 22-23 0.29558162004353505 1104 24-25 0.35988595448522886 1104 26-27 0.39664077741235104 1104 28-29 0.42131042601603497 1104 30-31 0.3753364840656772 1104 32-33 0.3448833702946672 1104 34-35 0.2328095737384217 1104 36-37 0.14670977624873416 1104 38-39 0.08420729955881967 1104 40-41 0.09504378577992867 1104 42-43 0.0838970175288054 1104 44-45 0.08241410630685664 1104 46-47 0.31503130286360204 1104 48-49 0.19561915002412178 1104 50-51 0.1126438319911145 1104 52-53 0.24736555683478656 1104 54-55 0.19156494554862746 1104 56-57 0.7180742675294809 1104 58-59 -8.741240731055267 1104 60-61 -7.765490259831029 1104 62-63 -2.1858159286692285 1104 64-65 -0.4613952744895542 1104 66-67 -1.7342048729533381 1104 68-69 -3.539127720348734 1104 70-71 -4.130221259908065 1104 72-73 -3.138275392933533 1104 74-75 -3.634911622307836 1104 76-77 -0.29102897061007127 1104 78-79 3.6388540185314255 1104 80-81 2.938322393214282 1104 82-83 1.43794738941029 1104 84-85 0.9308411038370998 1104 86-87 0.9303862090986357 1104 88-89 0.8070358398854403 1104 90-91 2.2945352343020886 1104 92-93 2.185916498216642 1104 94-95 -6.404648343149301 1104 96-97 -7.7719744970044236 1104 98-99 -5.595727099434832 1104 100-101 -5.074897629645278 1105 1 0.1454239392755987 1105 2 0.14570750827357415 1105 3 0.12060271686992508 1105 4 0.11159127458424933 1105 5 0.09589173422626374 1105 6 0.2038960981840816 1105 7 0.05506788416369801 1105 8 0.16164887573744835 1105 9 0.1903600802999108 1105 10-11 0.16493748752933612 1105 12-13 0.1200321764202954 1105 14-15 0.15667370159755478 1105 16-17 0.29135564841782724 1105 18-19 0.23574223512748205 1105 20-21 0.2331143600643486 1105 22-23 0.29311314251167886 1105 24-25 0.269012044357801 1105 26-27 0.1437118706383771 1105 28-29 0.21569037236076838 1105 30-31 0.22745754375716132 1105 32-33 0.08289980221955062 1105 34-35 0.1467069580442555 1105 36-37 0.210293612600168 1105 38-39 0.17153124322079094 1105 40-41 0.17281407417697636 1105 42-43 0.0704593917742855 1105 44-45 0.11120250335581972 1105 46-47 0.11083479045582578 1105 48-49 0.05396489113747549 1105 50-51 0.05570385881874529 1105 52-53 -0.034084141354348674 1105 54-55 -0.21326604036686803 1105 56-57 0.49778855324377247 1105 58-59 -8.530059644536149 1105 60-61 -7.891776644807553 1105 62-63 -2.3468501339006167 1105 64-65 -0.4790998351870748 1105 66-67 -1.9349892458574338 1105 68-69 -3.6837330188061443 1105 70-71 -4.051433868895321 1105 72-73 -3.041999068319182 1105 74-75 -3.8232758074185043 1105 76-77 -0.596344865982303 1105 78-79 3.2720947965327625 1105 80-81 2.7559649150117345 1105 82-83 1.261268985654425 1105 84-85 0.8237649804299885 1105 86-87 0.7233660883742914 1105 88-89 0.6544885561832245 1105 90-91 2.0034091444295186 1105 92-93 1.8645845062649293 1105 94-95 -6.453798242545679 1105 96-97 -8.066313195864248 1105 98-99 -6.042401814391237 1105 100-101 -5.608594142053059 1106 1 0.10047486915812698 1106 2 0.2085310492967487 1106 3 0.21780891952622028 1106 4 0.12945924046201895 1106 5 0.23618163416098525 1106 6 0.12818340508007964 1106 7 0.1732996829833695 1106 8 0.11579829847229917 1106 9 0.05647224941269258 1106 10-11 0.08028284843501154 1106 12-13 0.19586128709325124 1106 14-15 0.1771204624301035 1106 16-17 0.2716205152105573 1106 18-19 0.11657707695435704 1106 20-21 0.11848455223184828 1106 22-23 0.19419533241811848 1106 24-25 0.10528060331466094 1106 26-27 0.10512572724513092 1106 28-29 0.2764090882233887 1106 30-31 0.18429690770000917 1106 32-33 0.1454714416436076 1106 34-35 0.26152417797477057 1106 36-37 0.21832014414170686 1106 38-39 0.28186616243507245 1106 40-41 0.1259451235725706 1106 42-43 0.22344161061688084 1106 44-45 0.19855067263907245 1106 46-47 0.22426318692157565 1106 48-49 0.11893520353583398 1106 50-51 0.0695752701182002 1106 52-53 0.1817797151402445 1106 54-55 0.10505323986300397 1106 56-57 0.8633563188115332 1106 58-59 -7.981366706529069 1106 60-61 -7.2949005162412845 1106 62-63 -1.8805026622255276 1106 64-65 -0.08121968920527678 1106 66-67 0.7458324737579183 1106 68-69 -2.1058339633810625 1106 70-71 -2.356002753776515 1106 72-73 -0.9334637987306351 1106 74-75 -0.8138045988964677 1106 76-77 2.3681215310621724 1106 78-79 4.486350116636217 1106 80-81 3.5572019918764894 1106 82-83 1.8079111575262345 1106 84-85 1.5381616166576073 1106 86-87 1.3617808579280677 1106 88-89 1.211978983698149 1106 90-91 2.588935457267528 1106 92-93 2.0055909686737223 1106 94-95 -6.5209554780433905 1106 96-97 -7.764229792433632 1106 98-99 -5.628351402667832 1106 100-101 -4.82582349363636 1107 1 -0.006893125166389069 1107 2 0.014839957779571478 1107 3 0.06110475412640071 1107 4 0.07185814590440032 1107 5 0.058721413222507124 1107 6 0.019254652673069472 1107 7 0.1233199525688562 1107 8 0.08329601814392618 1107 9 0.06348552599118307 1107 10-11 0.031194979781922427 1107 12-13 0.012216308883054694 1107 14-15 0.11592658384493149 1107 16-17 0.07905185198971765 1107 18-19 0.04312009914955439 1107 20-21 0.006165711652137418 1107 22-23 0.00859940660480163 1107 24-25 0.10411256845097228 1107 26-27 0.09771719374963794 1107 28-29 0.08017669742578448 1107 30-31 0.07970077919083707 1107 32-33 0.09487348887174107 1107 34-35 0.054784540800348225 1107 36-37 0.08751544159787272 1107 38-39 0.15525473038886162 1107 40-41 0.02543838393539488 1107 42-43 -0.05324329867672617 1107 44-45 -0.0770700834164586 1107 46-47 0.09873617769891041 1107 48-49 0.04816394580804939 1107 50-51 0.15170509681325228 1107 52-53 0.044159364476421104 1107 54-55 0.07872304831541754 1107 56-57 0.5931678116665111 1107 58-59 -8.269397516299005 1107 60-61 -7.788984330977275 1107 62-63 -2.1268870749142863 1107 64-65 -0.26736036012855635 1107 66-67 0.825899870129664 1107 68-69 -2.4991323316794336 1107 70-71 -2.542733776377103 1107 72-73 -0.92111202007451 1107 74-75 -0.586688960911264 1107 76-77 2.504558644673196 1107 78-79 4.525168906541964 1107 80-81 3.4416425513170488 1107 82-83 1.7204605646408595 1107 84-85 1.17774558341549 1107 86-87 1.2135443118654337 1107 88-89 1.1165852470000637 1107 90-91 2.4533876717197423 1107 92-93 1.854534416530214 1107 94-95 -6.647194456456283 1107 96-97 -8.118174760509035 1107 98-99 -5.860065702860393 1107 100-101 -4.906265877339614 1108 1 -0.06507565271715521 1108 2 -0.015573154472875217 1108 3 -0.03134332136429663 1108 4 -0.07212450223908462 1108 5 -0.1648651907774692 1108 6 -0.012797145101416163 1108 7 -0.05093847572907606 1108 8 0.006775005684126256 1108 9 0.03914638534840975 1108 10-11 -0.09899520030263176 1108 12-13 -0.0720780247296986 1108 14-15 -0.1753727464934869 1108 16-17 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0.44528263309049976 2313 64-65 -0.32850187347433035 2313 66-67 -2.2075669710920884 2313 68-69 -4.781524505458716 2313 70-71 -3.476694187150027 2313 72-73 -2.929511856554516 2313 74-75 -3.8311590842367735 2313 76-77 0.3406106232985593 2313 78-79 2.882222461847835 2313 80-81 2.214198027732049 2313 82-83 0.6446055958400727 2313 84-85 0.19974888725502993 2313 86-87 0.1368824019920396 2313 88-89 0.2881094439463503 2313 90-91 2.030892679310547 2313 92-93 3.4556609990626654 2313 94-95 5.657320776986065 2313 96-97 1.3372002068872852 2313 98-99 2.919282206825745 2313 100-101 3.9467009321144495 2314 1 -0.4231475626425265 2314 2 -0.4074050989663043 2314 3 -0.5023423082718139 2314 4 -0.31378655258030363 2314 5 -0.24154459167381503 2314 6 -0.3829638629119714 2314 7 -0.35293465018035164 2314 8 -0.5839622413780248 2314 9 -0.44301182476616674 2314 10-11 -0.4712761705313895 2314 12-13 -0.5491706140331516 2314 14-15 -0.731684754057575 2314 16-17 -0.7228379079024094 2314 18-19 -0.5767209971448963 2314 20-21 -0.5920857415855423 2314 22-23 -0.5992783027612205 2314 24-25 -0.562856728049347 2314 26-27 -0.8137429472579569 2314 28-29 -0.7711535495388091 2314 30-31 -0.7318295393073271 2314 32-33 -0.7039959585356144 2314 34-35 -0.6488847545557732 2314 36-37 -0.7050764894315478 2314 38-39 -0.6581375058448415 2314 40-41 -0.6350641267321322 2314 42-43 -0.8670028966987005 2314 44-45 -0.79460642548257 2314 46-47 -0.7060611169927284 2314 48-49 -0.669833889538431 2314 50-51 -0.6088429804887809 2314 52-53 -0.6547225207893135 2314 54-55 -0.5055071033416922 2314 56-57 0.1679014359652129 2314 58-59 3.3356797149616746 2314 60-61 3.318108861000969 2314 62-63 0.6166077874238383 2314 64-65 -0.21359935940431996 2314 66-67 -2.091312356602959 2314 68-69 -4.888637798616129 2314 70-71 -3.8115031297483135 2314 72-73 -3.100856501542946 2314 74-75 -4.251588688888731 2314 76-77 0.4005522452149535 2314 78-79 3.2092800933679158 2314 80-81 2.143901357511485 2314 82-83 0.6319039134801976 2314 84-85 0.3004188351547761 2314 86-87 0.3998191457342095 2314 88-89 0.42697730016205426 2314 90-91 2.2078521636470008 2314 92-93 3.8491809991279773 2314 94-95 6.548267653419803 2314 96-97 3.441451792263294 2314 98-99 6.0439347425293555 2314 100-101 6.336303667663611 2315 1 -0.485249566352298 2315 2 -0.47082045918575943 2315 3 -0.5304443253140967 2315 4 -0.2925103646118927 2315 5 -0.3201752636157167 2315 6 -0.473256628191443 2315 7 -0.483128913200801 2315 8 -0.6129577449886341 2315 9 -0.7495042785648494 2315 10-11 -0.5195849102424503 2315 12-13 -0.5770501447954288 2315 14-15 -0.7416667947483901 2315 16-17 -0.7264897660378224 2315 18-19 -0.7075141294850695 2315 20-21 -0.7483773536027982 2315 22-23 -0.6872953229616456 2315 24-25 -0.5034961698670983 2315 26-27 -0.5574900516031889 2315 28-29 -0.5963566724295646 2315 30-31 -0.4423664520310453 2315 32-33 -0.5542660252148295 2315 34-35 -0.6034076110629556 2315 36-37 -0.4635272703833948 2315 38-39 -0.5248924413738223 2315 40-41 -0.43399636966049115 2315 42-43 -0.48073942461281405 2315 44-45 -0.5286886570782912 2315 46-47 -0.4408978853920118 2315 48-49 -0.5160362903903817 2315 50-51 -0.27264761878608823 2315 52-53 -0.2197669992964748 2315 54-55 -0.3112213238814334 2315 56-57 0.3091165818645436 2315 58-59 3.553479044419692 2315 60-61 3.587697132224239 2315 62-63 0.8005821715034855 2315 64-65 0.001111202194380212 2315 66-67 -1.8786163582728577 2315 68-69 -5.040463644355642 2315 70-71 -3.6369691010134186 2315 72-73 -3.044785323848906 2315 74-75 -0.4725307932922931 2315 76-77 2.1140655889754214 2315 78-79 3.852935192970108 2315 80-81 2.782765398395629 2315 82-83 0.971954297873161 2315 84-85 0.6274352316436627 2315 86-87 0.7146297324147 2315 88-89 0.8887832492118655 2315 90-91 2.4683828163400854 2315 92-93 3.939778328959303 2315 94-95 6.571346907282479 2315 96-97 4.448433965862591 2315 98-99 6.549936976558261 2315 100-101 6.715212905760591 2316 1 -0.46615326202745777 2316 2 -0.4744404628867187 2316 3 -0.5377372036053067 2316 4 -0.33589423370356997 2316 5 -0.6101076549660363 2316 6 -0.6826897861696608 2316 7 -0.5510779192280779 2316 8 -0.6609099893578829 2316 9 -0.7417983464510769 2316 10-11 -0.608184129075056 2316 12-13 -0.538518832011242 2316 14-15 -0.6900292841742157 2316 16-17 -0.7806006429015184 2316 18-19 -0.7169358871777831 2316 20-21 -0.7210645826394995 2316 22-23 -0.8786301146503419 2316 24-25 -0.812571953678038 2316 26-27 -0.7917303960570266 2316 28-29 -0.6750326862288603 2316 30-31 -0.7046911519887473 2316 32-33 -0.6382522589570314 2316 34-35 -0.5467027561935538 2316 36-37 -0.45751981558800026 2316 38-39 -0.6752952514615842 2316 40-41 -0.7527789183017006 2316 42-43 -0.7130549382534994 2316 44-45 -0.7778146351897419 2316 46-47 -0.8414436121023812 2316 48-49 -0.8679407989554662 2316 50-51 -0.831763055763993 2316 52-53 -0.7428823173148729 2316 54-55 -0.5800830136350541 2316 56-57 0.21722945460431475 2316 58-59 3.2449782315297675 2316 60-61 3.207736355815232 2316 62-63 0.5074370475212504 2316 64-65 -0.07895267244874304 2316 66-67 -2.1097274069669467 2316 68-69 -3.1821625842943106 2316 70-71 -3.3161336748740524 2316 72-73 -0.7409726718450997 2316 74-75 5.4447452654472634 2316 76-77 5.795005468165442 2316 78-79 4.671768134177682 2316 80-81 2.8329588728061204 2316 82-83 1.0375307227436252 2316 84-85 0.93394016846295 2316 86-87 0.7125826376700672 2316 88-89 0.6608279317221708 2316 90-91 2.241668312533381 2316 92-93 3.803154168284671 2316 94-95 6.375872490184033 2316 96-97 3.564718530206463 2316 98-99 5.888148750905305 2316 100-101 5.923555601647241 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 13.0 4 43.0 5 84.0 6 231.0 7 579.0 8 1296.0 9 2307.0 10 3692.0 11 5733.0 12 8439.0 13 11496.0 14 14224.0 15 19252.0 16 25441.0 17 33482.0 18 37497.0 19 38268.0 20 40932.0 21 42106.0 22 42509.0 23 43288.0 24 45914.0 25 49062.0 26 60884.0 27 85212.0 28 121704.0 29 162357.0 30 197858.0 31 221425.0 32 231757.0 33 231070.0 34 236041.0 35 253131.0 36 257095.0 37 198951.0 38 80550.0 39 5617.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.23218119249887 19.774513343145152 14.258712971606254 23.734592492749723 2 15.552237052647316 21.166645604654764 39.53213855362714 23.748978789070772 3 18.860482904502906 25.527977701695757 30.36499556333223 25.246543830469104 4 12.767970341115612 17.326820774602016 37.89551763879289 32.009691245489485 5 13.16308155644928 38.19695240700297 34.96173413056269 13.678231905985061 6 31.198492567838365 37.52716631085592 17.5888204001643 13.685520721141422 7 27.40684793913323 33.00066985911603 21.877591610886597 17.714890590864147 8 25.469186458727822 35.85136947490411 20.056208409620922 18.623235656747145 9 26.015769095789853 16.495013785145357 20.460921444247575 37.028295674817215 10-11 24.43295108889479 26.618588910806228 28.392505408347112 20.555954591951867 12-13 25.58902755107053 23.969442037966278 28.445076179567803 21.996454231395393 14-15 22.433034244481203 25.464961614617092 26.621043426078284 25.480960714823425 16-17 21.92367618571351 28.619926553878432 27.09638668929194 22.360010571116113 18-19 21.865112338164685 27.773928775644187 28.79886870777876 21.562090178412372 20-21 22.776918406034284 26.907567984293546 28.529004330236983 21.786509279435183 22-23 22.2745596619525 26.78886553201122 28.500476589456092 22.436098216580188 24-25 22.25904711193244 26.898200169920873 28.64907210888034 22.193680609266348 26-27 22.38069407754004 26.932094270169298 28.645736564892072 22.041475087398588 28-29 22.109521989181065 27.21701729620526 28.755964546231773 21.91749616838191 30-31 22.341395680282993 27.01152596090905 28.542317087972968 22.104761270834988 32-33 22.220039065454657 27.026887533314646 28.534849678088197 22.2182237231425 34-35 22.5670650482249 26.968411554755882 28.369287335702086 22.095236061317124 36-37 22.549490590299655 26.84355866632395 28.568000944772997 22.038949798603397 38-39 22.26359193739399 27.063790996494202 28.60021163387873 22.072405432233076 40-41 22.45165363037738 26.95384027850017 28.573449004572794 22.021057086549657 42-43 22.224555703402075 27.081082236508113 28.334571325256995 22.359790734832817 44-45 22.39191992173444 27.137357340282037 28.16676606620793 22.30395667177559 46-47 22.48582210757279 27.2121840634041 27.805769197199442 22.496224631823672 48-49 22.438045578192884 27.136872968566966 27.989814359814847 22.435267093425303 50-51 22.396291007452678 27.3117533474363 27.929253873715158 22.362701771395862 52-53 22.522016527665837 27.35628577340722 27.768782919726178 22.35291477920077 54-55 22.386494932608382 27.137729886188232 27.841105458616795 22.634669722586594 56-57 22.26200143896167 27.393840959117817 27.821718348185982 22.522439253734532 58-59 21.34758437771708 26.967854494669304 29.39676700625008 22.287794121363536 60-61 21.6905505829885 27.112973953811615 28.87590303488289 22.320572428317003 62-63 22.422855767911397 27.114164048875562 28.021686718255438 22.44129346495761 64-65 22.284157313668977 27.2459429806023 27.882483695840783 22.587416009887942 66-67 21.98143066563324 27.005920626044855 28.488883803305963 22.523764905015945 68-69 21.2232062940427 26.68918426431014 29.869078197387104 22.218531244260053 70-71 22.08275172551244 26.589233122988965 28.840260341688445 22.48775480981014 72-73 21.398022367270766 26.71539935407598 29.204516938519447 22.682061340133806 74-75 21.55757249331433 27.09081843882961 28.682870185107877 22.668738882748187 76-77 20.554347377875946 27.289201879617693 29.008216604245813 23.148234138260545 78-79 21.892586534811596 27.156003464130695 28.10908682858978 22.84232317246793 80-81 21.93404317706671 27.455915763042256 27.662096484601783 22.947944575289256 82-83 22.253727967260126 27.343670610905946 27.463761422908835 22.938839998925094 84-85 22.272369477382746 27.15534969079056 27.309825383737728 23.262455448088957 86-87 22.038295002144505 27.225525492924913 27.58667452095654 23.149504983974044 88-89 22.247633603436284 27.20318514094448 27.33692561004518 23.212255645574054 90-91 21.92360833110437 27.15549273583494 27.687962480533006 23.23293645252768 92-93 21.749891516298554 27.26917900140152 27.685378925511895 23.295550556788037 94-95 20.674341523148623 27.353888448028897 28.383183250298543 23.588586778523936 96-97 20.273276354472227 27.334275482583116 28.390258217767467 24.00218994517719 98-99 21.057234448881548 27.095483402534587 27.931790840575392 23.915491308008473 100-101 20.4139706920648 27.2147797126334 28.4119347178173 23.9593148774845 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 532.0 1 407.5 2 312.0 3 373.5 4 399.0 5 436.0 6 582.5 7 904.5 8 1293.5 9 1570.0 10 1686.0 11 1756.5 12 1890.0 13 1969.5 14 2117.0 15 2365.5 16 2570.0 17 2855.0 18 3224.0 19 3645.0 20 4230.5 21 5005.5 22 6058.0 23 7418.5 24 9122.0 25 11586.5 26 15025.0 27 19266.5 28 23755.0 29 28597.0 30 34220.5 31 40017.0 32 46138.0 33 52572.0 34 59032.5 35 65175.0 36 71535.0 37 77803.5 38 82095.0 39 85320.5 40 88849.0 41 92449.5 42 95833.5 43 99022.0 44 102151.0 45 105587.5 46 109406.5 47 113566.0 48 117395.5 49 115731.0 50 109383.5 51 103690.0 52 99146.0 53 94778.0 54 89114.5 55 82386.5 56 74612.0 57 66315.0 58 58322.0 59 50070.5 60 41537.0 61 33326.5 62 26040.0 63 19822.5 64 14628.0 65 10521.5 66 7389.0 67 5077.5 68 3286.0 69 2036.5 70 1279.5 71 838.0 72 551.0 73 353.0 74 212.5 75 115.0 76 66.0 77 32.0 78 10.5 79 3.5 80 6.0 81 6.0 82 2.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0244879421799821 2 0.013560909840949393 3 1.7796469607545134E-4 4 0.0 5 0.06485033524989446 6 0.0 7 0.0 8 7.118587843018054E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 4.627082097961735E-4 16-17 1.2457528725281594E-4 18-19 5.516905578338992E-4 20-21 0.0 22-23 0.0 24-25 1.067788176452708E-4 26-27 1.4237175686036108E-4 28-29 9.966022980225275E-4 30-31 0.00530334794304845 32-33 0.005641480865591807 34-35 0.0019042222480073292 36-37 0.0897831891700652 38-39 0.08326968129370367 40-41 0.08933827742987657 42-43 0.09186537611414798 44-45 0.09581619236702299 46-47 0.09022810091025382 48-49 0.08045783909571154 50-51 0.07470957941247447 52-53 0.06296390947149468 54-55 0.05141400069619789 56-57 0.06997571849686747 58-59 0.708388472728334 60-61 0.334751593317924 62-63 0.0030076033636751275 64-65 0.0022601516401582317 66-67 1.0837338132210685 68-69 2.335537485415793 70-71 2.3135054560416526 72-73 1.7772800302967098 74-75 1.270810301735583 76-77 0.7360975759072819 78-79 0.5613540408307961 80-81 0.038707321396410664 82-83 1.9576116568299648E-4 84-85 0.0015126999166413363 86-87 0.0014593105078187008 88-89 0.0 90-91 0.33076518412583383 92-93 0.05411906407654475 94-95 0.9423408621891224 96-97 1.6375065579990504 98-99 0.6338924509511501 100-101 0.5622794572503885 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2809546.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.0121910178567 #Duplication Level Percentage of deduplicated Percentage of total 1 70.30801744600211 35.86566016042261 2 15.131403817957645 15.43772123859963 3 5.829982641919777 8.922005644812016 4 2.903282627956313 5.924112319845298 5 1.668196208253873 4.254917181535542 6 1.0374171740875484 3.175255382985547 7 0.6788929823906652 2.4242272948876527 8 0.4748076959091632 1.9376784704373322 9 0.3538826799406404 1.6247097780338695 >10 1.5096316074586893 13.05423731324822 >50 0.06994898649359234 2.440751649644466 >100 0.03181426127439377 2.904200383528891 >500 0.0015354140468185622 0.5500183502021866 >1k 0.0010468732136396887 0.984187149453048 >5k 1.395830951519585E-4 0.5003176823637564 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7903 0.2812909986168584 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6150 0.21889657617280514 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4541 0.1616275369757249 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3858 0.13731755949181826 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2818 0.10030090270812438 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.067788176452708E-4 2 0.0 0.0 0.0 0.0 1.067788176452708E-4 3 0.0 0.0 0.0 0.0 1.067788176452708E-4 4 0.0 0.0 0.0 0.0 1.067788176452708E-4 5 0.0 7.118587843018053E-5 0.0 0.0 1.067788176452708E-4 6 0.0 7.118587843018053E-5 0.0 0.0 1.7796469607545134E-4 7 0.0 7.118587843018053E-5 0.0 0.0 1.7796469607545134E-4 8 0.0 7.118587843018053E-5 0.0 0.0 1.7796469607545134E-4 9 0.0 7.118587843018053E-5 0.0 0.0 1.7796469607545134E-4 10-11 0.0 1.4237175686036106E-4 0.0 0.0 1.7796469607545134E-4 12-13 0.0 1.9576116568299648E-4 0.0 1.7796469607545132E-5 2.8474351372072217E-4 14-15 0.0 2.3135410489808674E-4 0.0 7.118587843018053E-5 3.9152233136599296E-4 16-17 0.0 2.491505745056319E-4 0.0 1.067788176452708E-4 4.627082097961735E-4 18-19 0.0 2.491505745056319E-4 0.0 1.067788176452708E-4 5.33894088226354E-4 20-21 0.0 2.847435137207221E-4 0.0 3.203364529358124E-4 5.694870274414442E-4 22-23 0.0 2.847435137207221E-4 0.0 5.516905578338992E-4 6.050799666565345E-4 24-25 0.0 3.025399833282672E-4 0.0 9.610093588074372E-4 6.406729058716248E-4 26-27 0.0 3.203364529358124E-4 0.0 0.0016728681431092424 6.940623146942603E-4 28-29 0.0 3.203364529358124E-4 0.0 0.0034347186342562107 7.118587843018054E-4 30-31 0.0 3.203364529358124E-4 0.0 0.009200774787100834 7.296552539093504E-4 32-33 0.0 3.3813292254335756E-4 0.0 0.020839665910435352 7.830446627319859E-4 34-35 0.0 3.559293921509027E-4 0.0 0.037052249722908964 8.542305411621664E-4 36-37 0.0 3.9152233136599296E-4 0.0 0.0650994858244001 8.542305411621664E-4 38-39 0.0 4.271152705810832E-4 0.0 0.10640509178351235 8.720270107697116E-4 40-41 0.0 4.271152705810832E-4 0.0 0.15413522327094842 9.25416419592347E-4 42-43 0.0 4.271152705810832E-4 0.0 0.2023102664985731 9.966022980225273E-4 44-45 0.0 4.271152705810832E-4 0.0 0.2604691291760306 0.0010499917068451628 46-47 0.0 4.271152705810832E-4 0.0 0.3295372277229133 0.0011033811156677983 48-49 0.0 4.271152705810832E-4 0.0 0.4004739555785881 0.0011389740548828884 50-51 0.0 4.271152705810832E-4 0.0 0.4740267644665722 0.0011745669940979788 52-53 0.0 4.4491174018862836E-4 0.0 0.5483270250780731 0.0012635493421357044 54-55 0.0 4.627082097961735E-4 0.0 0.633856858011935 0.0012813458117432496 56-57 0.0 4.627082097961735E-4 0.0 0.7172867075321065 0.0012991422813507948 58-59 0.0 4.627082097961735E-4 0.0 0.7783641912252015 0.00131693875095834 60-61 0.0 4.983011490112638E-4 0.0 0.8300985283743352 0.0013703281597809754 62-63 3.5592939215090264E-5 5.160976186188088E-4 0.0 0.877526119878443 0.0014237175686036107 64-65 3.5592939215090264E-5 6.050799666565346E-4 0.0 0.9314672192589123 0.0014237175686036107 66-67 3.5592939215090264E-5 6.050799666565346E-4 0.0 0.9752287380238658 0.0014237175686036107 68-69 3.5592939215090264E-5 6.050799666565346E-4 0.0 1.030896094956267 0.0014237175686036107 70-71 7.118587843018053E-5 6.050799666565346E-4 0.0 1.115126785608778 0.001459310507818701 72-73 7.118587843018053E-5 6.050799666565346E-4 0.0 1.228116571147082 0.0014949034470337912 74-75 7.118587843018053E-5 6.050799666565346E-4 0.0 1.380667908622959 0.0015660893254639719 76-77 7.118587843018053E-5 6.050799666565346E-4 0.0 1.5782621106755328 0.001601682264679062 78-79 7.118587843018053E-5 6.050799666565346E-4 0.0 1.8285338627664398 0.001601682264679062 80-81 7.118587843018053E-5 6.406729058716248E-4 0.0 2.1244535594007004 0.0016728681431092424 82-83 7.118587843018053E-5 6.406729058716248E-4 0.0 2.4245198334535187 0.0018152398999696036 84-85 7.118587843018053E-5 6.406729058716248E-4 0.0 2.7004362982488987 0.0018152398999696036 86-87 7.118587843018053E-5 6.406729058716248E-4 0.0 2.954890220697579 0.001886425778399784 88-89 7.118587843018053E-5 6.406729058716248E-4 0.0 3.203293343479694 0.0019220187176148744 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 9370 0.0 22.664225 1 GTATCAA 15525 0.0 22.646059 1 TACATGG 9365 0.0 22.068085 2 ACATGGG 9160 0.0 20.961756 3 GGTATCA 11775 0.0 20.80053 1 TCAACGC 17460 0.0 19.859306 4 GAGTACT 7055 0.0 19.812857 12-13 ATCAACG 17640 0.0 19.710499 3 CAACGCA 17590 0.0 19.685715 5 AACGCAG 17800 0.0 19.439621 6 TATCAAC 17935 0.0 19.363092 2 AGTACTT 7500 0.0 18.605865 12-13 GTACCGT 330 6.6211214E-10 18.571249 6 GTACTTT 7850 0.0 17.986496 14-15 ACGCAGA 19125 0.0 17.723078 7 CGCAGAG 18920 0.0 17.641026 8 CATGGGG 4580 0.0 17.304728 4 CATGGGA 4860 0.0 17.278448 4 AGTACAT 7450 0.0 17.163717 2 AGAGTAC 14440 0.0 16.274574 10-11 >>END_MODULE