FastQCFastQC Report
Fri 27 May 2016
SRR939593_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939593_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2518565
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51810.20571237986710686No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT46260.18367602186165535No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37340.14825902845469544No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25930.10295545280745186No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG71800.027.4285681
TACATGG74050.026.211692
ACATGGG74200.025.6460193
GTATCAA123200.023.7273641
CAACGCA138900.020.943365
CATGGGA46550.020.898434
ATCAACG139550.020.811393
TCAACGC139350.020.8072054
TATCAAC141500.020.5924842
AACGCAG143200.020.4752886
GAGTACT52000.018.93362812-13
AGAGTAC87150.018.56547510-11
ACGCAGA155450.018.3123917
GGTATCA93800.018.0105361
CGCAGAG157000.017.8603388
GAGTACA59800.017.8550421
GTCCTAG13050.017.818251
CATGGGG38550.017.6031824
GTACTTT57250.017.44789914-15
AGTACTT54650.017.23413312-13
Produced by FastQC (version 0.11.5)