##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939593_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2518565 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.132498863440095 31.0 30.0 33.0 26.0 34.0 2 30.276487206008184 31.0 30.0 34.0 26.0 34.0 3 30.320870416288642 31.0 30.0 34.0 26.0 34.0 4 33.891521163837346 35.0 35.0 37.0 28.0 37.0 5 33.871645163019416 35.0 35.0 37.0 28.0 37.0 6 33.96707847524284 36.0 35.0 37.0 29.0 37.0 7 33.77796959776698 35.0 35.0 37.0 28.0 37.0 8 33.76700105020121 35.0 33.0 37.0 28.0 37.0 9 35.042517465302666 37.0 34.0 39.0 28.0 39.0 10-11 34.921010376940835 37.0 34.0 39.0 27.0 39.0 12-13 34.761799874134674 37.0 34.0 39.0 27.0 39.0 14-15 35.47321172969528 38.0 34.0 40.0 27.0 41.0 16-17 35.19917254468318 38.0 33.5 40.0 26.5 41.0 18-19 35.134453945004395 38.0 33.5 40.0 26.5 41.0 20-21 35.25268654968206 38.0 34.0 40.0 26.0 41.0 22-23 35.108200503064246 38.0 34.0 40.0 25.0 41.0 24-25 34.98561859630385 38.0 34.0 40.0 25.0 41.0 26-27 35.12935163476027 38.0 34.0 40.0 25.0 41.0 28-29 35.01631325774797 38.0 34.0 40.0 25.0 41.0 30-31 34.815136397115026 38.0 34.0 40.0 24.0 41.0 32-33 34.60878535991725 38.0 33.0 40.0 24.0 41.0 34-35 34.38742299682558 38.0 33.0 40.0 24.0 41.0 36-37 34.117342216698795 38.0 33.0 40.0 23.0 41.0 38-39 33.96789640132377 38.0 33.0 40.0 22.5 41.0 40-41 33.712755477821695 38.0 32.5 40.0 20.5 41.0 42-43 33.40636711778335 38.0 31.5 40.0 18.5 41.0 44-45 33.16967122150908 37.0 31.0 40.0 18.5 41.0 46-47 32.7598424102614 37.0 31.0 40.0 15.0 41.0 48-49 32.434365799572376 37.0 30.5 40.0 14.0 41.0 50-51 31.828271853218006 36.5 30.0 38.5 12.5 40.5 52-53 31.892507638278147 36.5 30.0 39.0 10.0 40.0 54-55 31.928661956312425 37.0 30.0 39.0 9.0 40.0 56-57 31.77011929412185 36.0 30.0 39.0 8.0 40.0 58-59 31.355813330209862 36.0 29.5 39.0 7.5 40.0 60-61 30.92669337499727 35.5 29.0 39.0 4.0 40.0 62-63 30.503580411861517 35.0 28.0 38.5 2.0 40.0 64-65 29.873104128739975 34.5 28.0 38.0 2.0 40.0 66-67 29.326621111625073 34.0 26.5 38.0 2.0 40.0 68-69 28.80072719981418 34.0 26.0 37.0 2.0 39.0 70-71 28.22907647807382 33.5 26.0 36.5 2.0 39.0 72-73 27.47830312102328 32.5 25.0 36.0 2.0 39.0 74-75 27.000508424440106 32.0 24.0 35.5 2.0 38.0 76-77 26.59803300689083 32.0 22.5 35.0 2.0 37.5 78-79 26.000130232890555 31.5 21.5 35.0 2.0 37.0 80-81 25.30995447804603 31.0 19.5 34.5 2.0 36.0 82-83 24.62085433570307 31.0 17.5 34.0 2.0 36.0 84-85 23.940173273272677 30.0 10.5 34.0 2.0 35.0 86-87 23.249378316620774 30.0 6.0 34.0 2.0 35.0 88-89 22.42600588033265 29.0 2.0 34.0 2.0 35.0 90-91 21.119730679970537 28.0 2.0 33.5 2.0 35.0 92-93 19.390148358291327 25.0 2.0 32.0 2.0 34.0 94-95 19.15246936251397 25.0 2.0 32.0 2.0 34.0 96-97 18.536106671854807 25.0 2.0 32.0 2.0 34.0 98-99 17.541679289595464 23.5 2.0 32.0 2.0 34.0 100-101 15.927690768354202 10.0 2.0 31.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.0883853211236243 1101 2 1.1452611733447995 1101 3 1.1866971017148344 1101 4 1.2727169842707298 1101 5 1.1067561602835312 1101 6 0.9695457885055134 1101 7 1.0291244962305583 1101 8 1.0962142023730124 1101 9 1.4101858405121774 1101 10-11 1.2028740802886162 1101 12-13 1.2283697694187836 1101 14-15 1.3503970099766818 1101 16-17 1.1669025567186324 1101 18-19 1.2649656276930727 1101 20-21 1.4599409299074253 1101 22-23 1.6217391805760784 1101 24-25 1.4159624440079241 1101 26-27 1.298530587269255 1101 28-29 1.376908677484323 1101 30-31 1.5962482716767639 1101 32-33 1.3915250528991692 1101 34-35 1.3825270454170138 1101 36-37 1.4909294919887017 1101 38-39 1.298758388657923 1101 40-41 1.3907821005647705 1101 42-43 1.3983702704433156 1101 44-45 1.2886299616004209 1101 46-47 1.2104131011628567 1101 48-49 1.2674742546749869 1101 50-51 1.369523813913748 1101 52-53 1.4042152626533522 1101 54-55 1.5040401752433823 1101 56-57 1.5207006398440548 1101 58-59 1.2938446670245014 1101 60-61 1.2112309249117779 1101 62-63 1.2248481301928535 1101 64-65 1.0406592448633774 1101 66-67 1.002186517313632 1101 68-69 0.8716562525998661 1101 70-71 0.9604898503308874 1101 72-73 0.9512316002406429 1101 74-75 0.9323471073603429 1101 76-77 0.38257703451889213 1101 78-79 0.7478591624168516 1101 80-81 0.5096645013534697 1101 82-83 0.4198786738704392 1101 84-85 0.02802546604779721 1101 86-87 -0.45700859535461547 1101 88-89 -1.0838413243291072 1101 90-91 -4.7291022934231925 1101 92-93 -9.643497451721329 1101 94-95 -8.003915047458973 1101 96-97 -4.729894256739165 1101 98-99 -3.53528018828602 1101 100-101 -3.1221406321099217 1102 1 0.9831140364216218 1102 2 0.8355704106659694 1102 3 0.8471466177448619 1102 4 0.963042532437477 1102 5 0.7671697924533021 1102 6 0.7468728432506921 1102 7 0.835848587947531 1102 8 0.7991366165738896 1102 9 1.1160181095628374 1102 10-11 0.9339378681997772 1102 12-13 0.9683895707852983 1102 14-15 1.1500990108281073 1102 16-17 1.1082615328759786 1102 18-19 1.0281087711300998 1102 20-21 0.9010617677194404 1102 22-23 1.0619001251262645 1102 24-25 0.9997422867387513 1102 26-27 0.9939481095756904 1102 28-29 1.0802130373189769 1102 30-31 1.0274735913285582 1102 32-33 1.0220727112869952 1102 34-35 1.163449554220783 1102 36-37 1.220800957088663 1102 38-39 0.974157882043059 1102 40-41 1.1780633925142254 1102 42-43 1.0363081011117217 1102 44-45 0.8285140567317981 1102 46-47 0.7783026462914364 1102 48-49 0.8117174232850957 1102 50-51 0.8446038322036493 1102 52-53 0.8700304488117538 1102 54-55 0.9837494833536198 1102 56-57 1.0537746851023009 1102 58-59 0.7728507058432257 1102 60-61 0.6873822939082324 1102 62-63 0.6763815399696824 1102 64-65 0.5164376391911176 1102 66-67 0.7376835930508712 1102 68-69 0.4211876691913723 1102 70-71 0.454380623122443 1102 72-73 0.3395077450309323 1102 74-75 0.2873572826095021 1102 76-77 -0.06890825826350522 1102 78-79 -0.13707951152227693 1102 80-81 -0.1779828059705899 1102 82-83 -0.0072792645909167675 1102 84-85 -0.5872932995256654 1102 86-87 -0.8100364591721032 1102 88-89 -1.5211416184136546 1102 90-91 -5.3015248943832525 1102 92-93 -10.557634170316291 1102 94-95 -9.171940951256518 1102 96-97 -5.8152595136594485 1102 98-99 -4.318694450598127 1102 100-101 -3.565369374864508 1103 1 0.7289258012677458 1103 2 0.6624218734489702 1103 3 0.7701194779572234 1103 4 0.7190101864118859 1103 5 0.7813251272826491 1103 6 0.5994215753663497 1103 7 0.7069648447011829 1103 8 0.5047968730414496 1103 9 0.6115457216966149 1103 10-11 0.548316561663519 1103 12-13 0.5590604364154856 1103 14-15 0.6987702579113346 1103 16-17 0.7040618988324852 1103 18-19 0.7956662647452362 1103 20-21 0.839735667093322 1103 22-23 0.7574370500783658 1103 24-25 0.6079509028092431 1103 26-27 0.6406248489866471 1103 28-29 0.8658812804595328 1103 30-31 0.9663216031536024 1103 32-33 0.9009960898880749 1103 34-35 0.7759609903616749 1103 36-37 0.7173625155627263 1103 38-39 0.6669641396609478 1103 40-41 0.5746172027234522 1103 42-43 0.5488129065259741 1103 44-45 0.50909109385492 1103 46-47 0.4457234277337392 1103 48-49 0.5108146014266453 1103 50-51 0.3266213760632901 1103 52-53 0.5443597768881858 1103 54-55 0.5784958617848375 1103 56-57 0.4152292281185801 1103 58-59 0.2833359707295813 1103 60-61 0.0028926065507199894 1103 62-63 0.23519541472792227 1103 64-65 0.158718281343468 1103 66-67 0.18317387940885865 1103 68-69 0.21650078849378573 1103 70-71 0.5065177286687117 1103 72-73 0.5384329712951654 1103 74-75 0.303434634133378 1103 76-77 0.1586969467824062 1103 78-79 0.17207513788363826 1103 80-81 0.0398103391991107 1103 82-83 -0.2064637597169643 1103 84-85 -0.16189240132296234 1103 86-87 -0.5842010621182325 1103 88-89 -1.1284629091553704 1103 90-91 -2.95614211519986 1103 92-93 -9.635613974165986 1103 94-95 -6.251276850457552 1103 96-97 -3.6406170513800884 1103 98-99 -2.571140071189438 1103 100-101 -2.1918549556416593 1104 1 0.8011949887561975 1104 2 0.698135039461544 1104 3 0.7833244001659452 1104 4 0.7607512665881302 1104 5 0.7075594584568208 1104 6 0.6450555784679253 1104 7 0.7381714912505473 1104 8 0.6477791425648505 1104 9 0.8287944466362944 1104 10-11 0.6812850588558632 1104 12-13 0.6375393496330091 1104 14-15 0.7501837413593435 1104 16-17 0.8895758979031356 1104 18-19 0.851919658841048 1104 20-21 0.8173585683524252 1104 22-23 0.7638295671118271 1104 24-25 0.6960483336536711 1104 26-27 0.7820154625271982 1104 28-29 0.8542808908033379 1104 30-31 0.8211570349945632 1104 32-33 0.8481215356693141 1104 34-35 0.8140729773283439 1104 36-37 0.8650584493659608 1104 38-39 0.6160478037728225 1104 40-41 0.5453484799112118 1104 42-43 0.5868332179150215 1104 44-45 0.5881112428872228 1104 46-47 0.3554933850171267 1104 48-49 0.3627390887432185 1104 50-51 0.3039609927894027 1104 52-53 0.4070464171672086 1104 54-55 0.46002722183051503 1104 56-57 0.44723268239836145 1104 58-59 0.37074870397744775 1104 60-61 0.2085883891942224 1104 62-63 0.05533898847987473 1104 64-65 0.30088372846835654 1104 66-67 0.32142051050633214 1104 68-69 0.15948291805447212 1104 70-71 0.13828800506707495 1104 72-73 0.20209613142930394 1104 74-75 0.05533728726658538 1104 76-77 0.010511563368545751 1104 78-79 -0.06250447010879512 1104 80-81 -0.32888895930776485 1104 82-83 -0.2978081855619159 1104 84-85 -0.42495568126518535 1104 86-87 -0.5426382944395414 1104 88-89 -0.6025705068913325 1104 90-91 -0.9284969598692392 1104 92-93 -4.30738139532499 1104 94-95 -1.906668354160967 1104 96-97 -1.1652916380701939 1104 98-99 -0.9803664132919003 1104 100-101 -0.874045120181826 1105 1 0.9218672999306463 1105 2 0.973334380661683 1105 3 0.9621939863942686 1105 4 0.9581274087626639 1105 5 0.8309118424148494 1105 6 0.8107984788638944 1105 7 0.9011850074961743 1105 8 0.8880685016650176 1105 9 1.0661275892990076 1105 10-11 0.7839513244567513 1105 12-13 0.7031394936221957 1105 14-15 1.0346684129209862 1105 16-17 1.0706447710890927 1105 18-19 1.0650470843439024 1105 20-21 0.8955663960387881 1105 22-23 0.9576207729226809 1105 24-25 0.8671945270627148 1105 26-27 0.9959244731932415 1105 28-29 1.072254390874832 1105 30-31 1.2165885834207941 1105 32-33 1.1874342831507434 1105 34-35 1.0913051796232978 1105 36-37 0.9330415651539781 1105 38-39 0.9838230721499386 1105 40-41 0.966301126205714 1105 42-43 0.9405917302897038 1105 44-45 0.9238587785516756 1105 46-47 1.018133367659523 1105 48-49 0.7898063445163501 1105 50-51 0.8222690704843849 1105 52-53 0.801081221129877 1105 54-55 0.9906310888279037 1105 56-57 0.967439247713731 1105 58-59 0.8856999103800334 1105 60-61 0.7439717604151213 1105 62-63 0.9252223177861119 1105 64-65 0.9522868834095988 1105 66-67 0.8029225840493055 1105 68-69 0.6556612871303251 1105 70-71 0.746172646055669 1105 72-73 0.7000743653920161 1105 74-75 0.8552194331000216 1105 76-77 0.6702101229835193 1105 78-79 0.5538814505097669 1105 80-81 0.3248010102671195 1105 82-83 0.5004023049382482 1105 84-85 0.35215694096536154 1105 86-87 0.09130617109920891 1105 88-89 -0.2816928138743151 1105 90-91 -1.376630031523586 1105 92-93 -7.399328602575732 1105 94-95 -3.0650749559667467 1105 96-97 -1.3043570890473006 1105 98-99 -0.6180831983880353 1105 100-101 -0.428748684900528 1106 1 0.7969943006655384 1106 2 0.7734182602168218 1106 3 0.8302160646806414 1106 4 0.8612183506794793 1106 5 0.6086944837999155 1106 6 0.7117194273056882 1106 7 0.734266964697909 1106 8 0.7931023957907826 1106 9 1.0115240890769641 1106 10-11 0.7653057687403049 1106 12-13 0.8344387149266979 1106 14-15 0.7813533680973777 1106 16-17 0.9385311395859972 1106 18-19 0.9760332007477572 1106 20-21 0.8176969973240276 1106 22-23 1.0459586066915207 1106 24-25 0.8723453648586172 1106 26-27 0.8948087145603765 1106 28-29 0.8318604780647405 1106 30-31 0.8031031955482817 1106 32-33 1.0207001907311621 1106 34-35 0.9836943595544838 1106 36-37 0.9977101576071803 1106 38-39 0.9427595769321968 1106 40-41 0.9317650295221753 1106 42-43 0.926537739604008 1106 44-45 0.7852928735763953 1106 46-47 0.6739279787691856 1106 48-49 0.6332920625756273 1106 50-51 0.6699667145260939 1106 52-53 0.750163165608825 1106 54-55 0.8730166700262814 1106 56-57 0.8771476322159195 1106 58-59 0.8595625987303777 1106 60-61 0.6437303151300959 1106 62-63 0.617048862499761 1106 64-65 0.6227037699483411 1106 66-67 0.3144982049973244 1106 68-69 0.5029315816508664 1106 70-71 0.6208364765492966 1106 72-73 0.8358110393268312 1106 74-75 0.7849706085951915 1106 76-77 0.5595116780117202 1106 78-79 0.519008275548952 1106 80-81 0.49621534861222827 1106 82-83 0.5388840577271132 1106 84-85 0.39421092074581665 1106 86-87 0.34168558295478846 1106 88-89 0.23923754337564773 1106 90-91 -0.20401013283336766 1106 92-93 -3.584915299017773 1106 94-95 -1.2318878842629069 1106 96-97 -0.4126250262160873 1106 98-99 -0.257436098972736 1106 100-101 -0.30812297795248966 1107 1 0.607745467665918 1107 2 0.6423150394558661 1107 3 0.5656218155778667 1107 4 0.5004068251685183 1107 5 0.36706048168084493 1107 6 0.45055799437804467 1107 7 0.3098362053368575 1107 8 0.3678886952389604 1107 9 0.39554799768777826 1107 10-11 0.3725097170644176 1107 12-13 0.4765889364685236 1107 14-15 0.536843591263775 1107 16-17 0.6771952421029752 1107 18-19 0.4784784467442691 1107 20-21 0.4692465153156675 1107 22-23 0.4479734129068973 1107 24-25 0.47770809697652084 1107 26-27 0.37511586111475026 1107 28-29 0.3517880508166087 1107 30-31 0.439491565803273 1107 32-33 0.2623450876206874 1107 34-35 0.3029024624177268 1107 36-37 0.4086684586514835 1107 38-39 0.3897737131562593 1107 40-41 0.34210722018036677 1107 42-43 0.17202715640916466 1107 44-45 -0.03625745670561287 1107 46-47 0.08350961069018581 1107 48-49 0.2372661190885097 1107 50-51 0.313468999315095 1107 52-53 0.4864545057041241 1107 54-55 0.3795186245629196 1107 56-57 0.2342038711692105 1107 58-59 0.324425852314981 1107 60-61 0.2663906817042907 1107 62-63 0.11574163827382478 1107 64-65 0.20337228195689505 1107 66-67 0.14026607138326241 1107 68-69 0.2618400946317969 1107 70-71 0.12627793264796594 1107 72-73 0.3487914542352648 1107 74-75 0.2899065325221244 1107 76-77 0.08628954149832069 1107 78-79 0.10414045653673298 1107 80-81 -0.015446799071650474 1107 82-83 -0.08495005360646957 1107 84-85 -0.03994227247434523 1107 86-87 -0.22782097732753215 1107 88-89 -0.39948608757415727 1107 90-91 -0.6436685943791254 1107 92-93 -4.298102759033224 1107 94-95 -1.9960623511899378 1107 96-97 -1.0313145824743124 1107 98-99 -0.7328202915650053 1107 100-101 -0.7172380617103009 1108 1 0.4195494191359437 1108 2 0.4533937885307928 1108 3 0.16833929219870924 1108 4 -0.22461265746250803 1108 5 -0.8515944879318624 1108 6 -0.09112740738463998 1108 7 0.10398496854019612 1108 8 0.1364006987822748 1108 9 0.27661648684478024 1108 10-11 0.22374364038974903 1108 12-13 0.2949295208913796 1108 14-15 0.2386005321262772 1108 16-17 0.3222383141354541 1108 18-19 0.24560683307782938 1108 20-21 0.06491534422733736 1108 22-23 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2311 74-75 0.4246095744350704 2311 76-77 0.45385736257659204 2311 78-79 0.4383957947452686 2311 80-81 0.5557405649848981 2311 82-83 0.7497132467978496 2311 84-85 0.6891136641912468 2311 86-87 0.8027058029788492 2311 88-89 0.8740751355136602 2311 90-91 1.631513578812008 2311 92-93 2.719382309423846 2311 94-95 1.9376051895742883 2311 96-97 1.5999970715332452 2311 98-99 1.472964010482837 2311 100-101 1.3863307567026482 2312 1 0.33454496280796064 2312 2 0.08307293291581885 2312 3 0.11176620841974128 2312 4 0.11151702168187683 2312 5 0.18631772240681954 2312 6 0.11423132701821004 2312 7 0.32225591328172953 2312 8 -0.39431454185943693 2312 9 0.14816283265583508 2312 10-11 0.03453352452521585 2312 12-13 0.20349681144396925 2312 14-15 -0.1895300382836993 2312 16-17 0.054385150142579164 2312 18-19 0.16607808976232263 2312 20-21 0.3867067880776034 2312 22-23 0.20712519088493053 2312 24-25 0.3222779135178442 2312 26-27 0.25508429305135394 2312 28-29 0.19014079287693164 2312 30-31 0.5693262739214262 2312 32-33 0.4250989607014688 2312 34-35 0.33459479077178855 2312 36-37 0.5559518867178852 2312 38-39 0.6126550715360821 2312 40-41 0.6297208709850182 2312 42-43 0.724287103381748 2312 44-45 0.4663905926017975 2312 46-47 0.5077449588342233 2312 48-49 0.5457157748146599 2312 50-51 0.5951720140218235 2312 52-53 0.5393258853411353 2312 54-55 0.5863649663644708 2312 56-57 0.285826175031314 2312 58-59 0.6094167977972518 2312 60-61 0.6975753361018668 2312 62-63 0.6761757063661094 2312 64-65 0.7088621068162411 2312 66-67 0.451171999709608 2312 68-69 0.35059169758469366 2312 70-71 0.5291182640981944 2312 72-73 0.569420952612095 2312 74-75 1.036458956302436 2312 76-77 1.1608315446406863 2312 78-79 1.1742111597565028 2312 80-81 1.006397261049191 2312 82-83 1.2143553334705537 2312 84-85 1.1286635339698172 2312 86-87 1.1499225993080593 2312 88-89 1.484693561566889 2312 90-91 2.0754852486643465 2312 92-93 3.2360111112748875 2312 94-95 2.37275624364133 2312 96-97 1.9745857282963755 2312 98-99 1.8535195893254865 2312 100-101 1.780205878261281 2313 1 -0.37760925572592896 2313 2 -1.0911301979781847 2313 3 -0.5482976039780958 2313 4 -0.3773692476504493 2313 5 -0.9395608768638937 2313 6 -0.5167632662170476 2313 7 -1.0741683920118703 2313 8 -0.9548765939152659 2313 9 -1.3429238641881227 2313 10-11 -2.1302572776877824 2313 12-13 -0.7384346923910599 2313 14-15 -0.3338497918358172 2313 16-17 -1.5016966743261762 2313 18-19 -1.2996659690833994 2313 20-21 -1.5533606393082877 2313 22-23 -1.0957392942150364 2313 24-25 -0.42738652128664256 2313 26-27 -0.9512922315305659 2313 28-29 -2.3364309410599446 2313 30-31 -0.731256839369145 2313 32-33 -0.25410228411180213 2313 34-35 -0.22545094668577548 2313 36-37 -0.04485205519082314 2313 38-39 -0.325319592112848 2313 40-41 -0.37730163624495816 2313 42-43 -0.0881398109901852 2313 44-45 -0.5906932122517219 2313 46-47 -0.7716731852623475 2313 48-49 -0.39945035746247015 2313 50-51 -0.3267780362736623 2313 52-53 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-0.5473735423181303 2314 74-75 -0.42850576627627035 2314 76-77 -0.4774957621933922 2314 78-79 -0.42536048187923825 2314 80-81 -0.5016854739698253 2314 82-83 -0.26158520507013705 2314 84-85 -0.36682107172081757 2314 86-87 -0.17365409186925973 2314 88-89 -0.02423570149485954 2314 90-91 0.4634945384334621 2314 92-93 1.4651303752615696 2314 94-95 1.0509255030033593 2314 96-97 0.6847984044667008 2314 98-99 0.45509912466360447 2314 100-101 0.7163076437220681 2315 1 -7.1854530946901 2315 2 -3.000114712771829 2315 3 -1.4181580340044988 2315 4 -0.955606132941277 2315 5 -1.3221418735527735 2315 6 -1.5825052697183892 2315 7 -2.923460869935056 2315 8 -2.6304062279511236 2315 9 -3.3529152559222553 2315 10-11 -2.2286123573706647 2315 12-13 -2.827053887856728 2315 14-15 -4.441258865012529 2315 16-17 -4.280487615980185 2315 18-19 -3.3830186468344117 2315 20-21 -4.353498145877332 2315 22-23 -5.6674426024328675 2315 24-25 -3.7374880149980925 2315 26-27 -4.525558675091617 2315 28-29 -2.7818289040927624 2315 30-31 -2.796417704322547 2315 32-33 -1.8915774184749026 2315 34-35 -2.6265211765941743 2315 36-37 -3.2539208794048804 2315 38-39 -2.2861697147886986 2315 40-41 -3.3855471942830455 2315 42-43 -7.546123735779087 2315 44-45 -4.231365817654616 2315 46-47 -2.9542111406218794 2315 48-49 -5.552259166910282 2315 50-51 -6.659086921487109 2315 52-53 -6.980133127867877 2315 54-55 -4.568539851040349 2315 56-57 -3.788474524269386 2315 58-59 -4.160226753096296 2315 60-61 -3.201113475086945 2315 62-63 -3.279700362009958 2315 64-65 -2.1241944199902854 2315 66-67 -2.863751271463663 2315 68-69 -2.2024386054456073 2315 70-71 -1.8157195369896115 2315 72-73 -2.289917625476484 2315 74-75 -2.880348541755062 2315 76-77 -2.9025182999591586 2315 78-79 -2.1550429194975784 2315 80-81 -1.8237503690984376 2315 82-83 -2.171211877096656 2315 84-85 -1.888469333163055 2315 86-87 -2.272576429440967 2315 88-89 -2.027005156381829 2315 90-91 -1.0815368958577984 2315 92-93 -0.27852851576473725 2315 94-95 -0.2356499335148463 2315 96-97 -0.19396516525452157 2315 98-99 -0.2964755544196578 2315 100-101 -0.22445612640072454 2316 1 -4.385984395221399 2316 2 -2.8448069574640726 2316 3 -4.590866406712873 2316 4 -4.293139470474614 2316 5 -3.8398595912704927 2316 6 -4.253178740391856 2316 7 -3.1452399917141776 2316 8 -1.7554277529726505 2316 9 -1.94873205173905 2316 10-11 -3.2597133884716953 2316 12-13 -4.220366431169275 2316 14-15 -3.275372349126009 2316 16-17 -3.2927652782578463 2316 18-19 -3.330971144786915 2316 20-21 -5.523653966033152 2316 22-23 -6.310960945951209 2316 24-25 -4.370324697834775 2316 26-27 -4.090163139696081 2316 28-29 -4.880761053024912 2316 30-31 -6.525201383106225 2316 32-33 -4.499293876191363 2316 34-35 -5.973056173129173 2316 36-37 -9.372536348020347 2316 38-39 -6.331338659957645 2316 40-41 -5.572247880983731 2316 42-43 -4.759675049330404 2316 44-45 -3.5297026159914147 2316 46-47 -5.706815143225882 2316 48-49 -3.140890805541922 2316 50-51 -4.478611691011878 2316 52-53 -5.394285042019792 2316 54-55 -7.026026358526856 2316 56-57 -4.985428471223333 2316 58-59 -5.8842812271507725 2316 60-61 -6.128751602725071 2316 62-63 -6.413963996273594 2316 64-65 -5.054497450293315 2316 66-67 -6.10058215829455 2316 68-69 -5.029289232296232 2316 70-71 -5.988083509353579 2316 72-73 -7.980099065657921 2316 74-75 -7.652085663492183 2316 76-77 -6.937726935167795 2316 78-79 -6.011906776361432 2316 80-81 -5.914008459356527 2316 82-83 -5.075893831778611 2316 84-85 -6.554776899470621 2316 86-87 -5.049906906771447 2316 88-89 -4.995782325158995 2316 90-91 -5.4036152679361695 2316 92-93 -4.284875772111995 2316 94-95 -4.280921778786691 2316 96-97 -4.914015985305339 2316 98-99 -5.999136257080361 2316 100-101 -4.974934926879525 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14861.0 3 5946.0 4 6159.0 5 6709.0 6 7761.0 7 8885.0 8 10661.0 9 12936.0 10 15505.0 11 17750.0 12 20669.0 13 22796.0 14 24927.0 15 27521.0 16 30489.0 17 33591.0 18 32433.0 19 32878.0 20 35087.0 21 38264.0 22 42304.0 23 46889.0 24 52603.0 25 59014.0 26 66602.0 27 75241.0 28 84860.0 29 96709.0 30 110606.0 31 126967.0 32 145428.0 33 169187.0 34 198655.0 35 239182.0 36 277279.0 37 244490.0 38 75487.0 39 1234.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.86106652476751 18.930105259978674 13.417895269361345 23.790932945892468 2 15.293379797587972 20.47613407650704 40.27911143472164 23.951374691183343 3 19.3614629923876 24.621506280607182 29.587450390635244 26.429580336369973 4 12.612426781003164 16.575771377880372 37.90782653347546 32.90397530764101 5 13.742090198562742 37.41617259800762 34.7293637741626 14.112373429267041 6 31.866645897723018 36.47222537906634 17.834177998854 13.826950724356646 7 28.20537990202897 32.467588725144594 21.571232474940924 17.75579889788551 8 25.72011391156514 35.16912274901067 20.295564427354353 18.815198912069842 9 26.347556425187502 16.707688957519874 20.41137794365071 36.53337667364192 10-11 24.7438418927345 26.35470809123016 28.176418153992522 20.72503186204282 12-13 25.97686404436915 23.7844165123753 28.08993050629425 22.148788936961296 14-15 22.769220986449778 25.2087775391017 26.25776221636241 25.764239258086118 16-17 22.555651729501637 28.380732781883054 26.64922421008205 22.41439127853326 18-19 22.4006120309791 27.57124050532298 28.355971524662564 21.672175939035355 20-21 23.164286763301615 26.63878072952966 28.246614070514138 21.950318436654587 22-23 22.812448635674066 26.67429069778766 28.070525865360324 22.44273480117795 24-25 22.856010874755437 26.76606433806208 28.121075086782948 22.256849700399535 26-27 22.685880076292747 26.843413388764993 28.173009750505855 22.297696784436408 28-29 22.417649224622807 27.19219700409617 28.193932964723338 22.196220806557683 30-31 22.652981336613912 26.803232360021624 28.099313539995652 22.444472763368815 32-33 22.464778540375953 26.928621923862643 28.16195947176359 22.444640063997813 34-35 22.97204806640828 26.861674794158862 27.910645096459426 22.25563204297343 36-37 22.814394279757213 26.751553990278072 28.03365063865283 22.40040109131189 38-39 22.569133087492343 27.00850371812795 28.213026497910892 22.209336696468807 40-41 22.81001312913764 26.831087333115345 28.068883745014457 22.29001579273256 42-43 22.55052503337923 26.947439812531666 27.81624398234643 22.685791171742675 44-45 22.801110582634514 26.824001194140678 27.768382024843614 22.606506198381194 46-47 22.874303421821025 27.074566467553453 27.53276939127337 22.518360719352152 48-49 22.935029197791426 27.106088291258157 27.487717442523994 22.471165068426423 50-51 22.53430973001016 27.182023664104378 27.69274485197457 22.59092175391089 52-53 22.928787835098085 27.33509460452435 27.20740410037456 22.528713460003004 54-55 22.62116756838019 26.96289482156532 27.45866326065735 22.95727434939714 56-57 22.754908737549854 27.25762564751878 27.392591452801828 22.59487416212954 58-59 23.013788000888958 27.047377592282125 27.300726791165914 22.638107615663003 60-61 22.832225915272232 27.022975205987315 27.685815951471454 22.458982927269 62-63 22.699858554360368 27.01295670682111 27.656617851091692 22.63056688772683 64-65 22.560388585692763 27.152683650066184 27.479062242874008 22.807865521367045 66-67 22.54792052529411 27.28938602653106 27.183222836952343 22.97947061122249 68-69 22.499767444264144 27.16529266642444 27.420587185344136 22.914352703967282 70-71 22.62019014783887 26.97950070488442 27.390544132602557 23.00976501467416 72-73 22.67275014569451 26.847954182630428 27.52531684455216 22.9539788271229 74-75 22.53586246504036 27.25555297479088 27.19159950491742 23.016985055251343 76-77 22.6853170272274 26.934387916469127 27.25516181482059 23.125133241482885 78-79 22.60013502804467 27.124926793521436 27.20465101017483 23.070287168259064 80-81 22.620760536473522 27.11852609192058 27.203524676934776 23.057188694671122 82-83 22.637300440147357 27.09698435818274 27.26109496698298 23.00462023468692 84-85 22.561807663010917 27.075159366870977 27.130553285032327 23.232479685085774 86-87 22.49669889005957 26.987580350537016 27.340726043366477 23.17499471603694 88-89 22.535551875927503 26.92468169073915 27.136642585564942 23.403123847768402 90-91 22.260351074297382 26.879579999159965 27.570378188270624 23.289690738272025 92-93 22.094898450270502 26.964259230019767 27.868941793671613 23.07190052603812 94-95 22.554482551244913 26.838489660530996 27.140258450430988 23.46676933779311 96-97 22.48451402767347 26.7901354396059 27.229295815883603 23.49605471683703 98-99 22.483796363526412 26.82015644451376 26.856420920962538 23.83962627099729 100-101 22.470320602152334 26.717575475442317 27.09645717264134 23.71564674976401 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2012.0 1 1173.5 2 353.5 3 351.0 4 319.0 5 320.0 6 402.5 7 695.0 8 1110.0 9 1352.5 10 1278.0 11 1150.0 12 1218.0 13 1352.5 14 1476.5 15 1586.0 16 1742.0 17 1956.0 18 2102.5 19 2349.0 20 2698.0 21 3143.5 22 3839.0 23 4811.5 24 5912.5 25 7634.5 26 10336.5 27 13609.0 28 17272.5 29 21464.0 30 26359.0 31 31828.0 32 37031.0 33 42215.5 34 48394.5 35 54097.0 36 59825.5 37 66395.5 38 70873.0 39 73292.0 40 75671.0 41 78646.5 42 82371.0 43 86806.0 44 90096.5 45 91826.0 46 94225.5 47 99175.0 48 107523.0 49 108753.5 50 102391.5 51 97748.5 52 93657.5 53 90227.5 54 86311.0 55 80172.0 56 73392.5 57 66020.0 58 58457.5 59 51079.0 60 42883.0 61 34229.5 62 26752.0 63 20966.5 64 15884.5 65 11598.5 66 8330.0 67 6002.0 68 4257.0 69 2865.0 70 1900.0 71 1288.0 72 883.5 73 595.0 74 414.0 75 276.0 76 169.5 77 117.5 78 73.5 79 41.5 80 28.0 81 22.0 82 19.5 83 16.0 84 11.5 85 13.0 86 10.0 87 4.5 88 3.5 89 3.0 90 6.0 91 4.5 92 5.0 93 5.5 94 1.5 95 1.0 96 1.5 97 2.5 98 2.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08830425262004356 2 0.0996202202444646 3 0.09557029498940865 4 0.09858788635592093 5 0.09461737139998372 6 0.07889413217447237 7 0.09175860063170893 8 0.08842336806872168 9 0.0812764411480347 10-11 0.08925717620946849 12-13 0.08822484232092481 14-15 0.09410120445571188 16-17 0.09096449764052149 18-19 0.09340636433842288 20-21 0.10329294657870652 22-23 0.11258395157559961 24-25 0.13428281580979645 26-27 0.1396033058507523 28-29 0.1401988830941429 30-31 0.14137018500614437 32-33 0.13813421531705555 34-35 0.13247623150484503 36-37 0.1360695475399682 38-39 0.12918070409141713 40-41 0.12576606122931114 42-43 0.12521018913547993 44-45 0.11707063347580864 46-47 0.135513675446137 48-49 0.15167367131680143 50-51 0.16205656792657724 52-53 0.17003730298801104 54-55 0.16618590348075193 56-57 0.13831288849007273 58-59 0.1295181978626718 60-61 0.12624252302402358 62-63 0.1235227202792066 64-65 0.11018179002725759 66-67 0.12300655333493478 68-69 0.12066394951093182 70-71 0.1293990824139937 72-73 0.1542743586129403 74-75 0.1682307186830596 76-77 0.17204241304075932 78-79 0.1696402514924173 80-81 0.1749210363838138 82-83 0.17498059410815286 84-85 0.15280526807924355 86-87 0.15407583286514345 88-89 0.17557617135154344 90-91 0.2684266635961351 92-93 0.5442980427346524 94-95 0.17424604884130449 96-97 0.18570098448918332 98-99 0.20194039065896652 100-101 0.20835277231280508 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2518565.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.80413114960726 #Duplication Level Percentage of deduplicated Percentage of total 1 79.62610637296751 49.21222321207462 2 11.596856225121964 14.334672461211543 3 3.8110588230679245 7.066175379592736 4 1.74266043634894 4.308144566293668 5 0.9226469105496833 2.851169533219629 6 0.547769434518819 2.0312648382448373 7 0.3510354960368038 1.518681068465824 8 0.2568058492079894 1.269732990754947 9 0.17966619998188293 0.9993702048148673 >10 0.8843773831808436 9.665470575076746 >50 0.05217478228637728 2.2356261266288744 >100 0.02669907625541303 2.852725631313362 >500 0.0014286736505116598 0.5769868610821135 >1k 6.49397113857592E-4 0.8698149174457689 >5k 6.493971138575919E-5 0.20794163378029384 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5181 0.20571237986710686 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4626 0.18367602186165535 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3734 0.14825902845469544 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2593 0.10295545280745186 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 3.97051495593721E-5 0.0 4 0.0 0.0 0.0 3.97051495593721E-5 0.0 5 0.0 0.0 0.0 3.97051495593721E-5 0.0 6 0.0 0.0 0.0 3.97051495593721E-5 0.0 7 0.0 0.0 0.0 3.97051495593721E-5 0.0 8 0.0 0.0 0.0 3.97051495593721E-5 0.0 9 0.0 0.0 0.0 1.1911544867811631E-4 0.0 10-11 3.97051495593721E-5 0.0 0.0 1.1911544867811631E-4 0.0 12-13 3.97051495593721E-5 0.0 0.0 1.1911544867811631E-4 0.0 14-15 1.3896802345780238E-4 0.0 0.0 1.1911544867811631E-4 0.0 16-17 2.1837832257654656E-4 0.0 0.0 1.1911544867811631E-4 0.0 18-19 2.580834721359187E-4 0.0 0.0 1.3896802345780235E-4 0.0 20-21 2.779360469156047E-4 0.0 0.0 2.1837832257654656E-4 0.0 22-23 3.176411964749768E-4 0.0 0.0 3.9705149559372105E-4 0.0 24-25 4.7646179471246523E-4 0.0 0.0 6.154298181702677E-4 0.0 26-27 5.558720938312094E-4 0.0 0.0 0.0010124813137639887 0.0 28-29 5.558720938312094E-4 3.97051495593721E-5 0.0 0.002322751249223268 0.0 30-31 5.955772433905816E-4 3.97051495593721E-5 0.0 0.006650612551194827 0.0 32-33 5.955772433905816E-4 3.97051495593721E-5 0.0 0.018264368797311165 0.0 34-35 6.749875425093258E-4 3.97051495593721E-5 0.0 0.03422583892017875 0.0 36-37 7.543978416280699E-4 3.97051495593721E-5 0.0 0.058545243025294166 0.0 38-39 7.941029911874421E-4 3.97051495593721E-5 0.0 0.09656292372839295 0.0 40-41 7.941029911874421E-4 3.97051495593721E-5 0.0 0.1369232082554947 0.0 42-43 8.139555659671281E-4 3.97051495593721E-5 0.0 0.18089666139249932 0.0 44-45 8.536607155265003E-4 3.97051495593721E-5 0.0 0.23509419054104222 0.0 46-47 9.529235894249305E-4 3.97051495593721E-5 0.0 0.2974511279240361 0.0 48-49 9.529235894249305E-4 3.97051495593721E-5 0.0 0.3655851645679187 0.0 50-51 9.529235894249305E-4 3.97051495593721E-5 0.0 0.4399529096926226 0.0 52-53 9.926287389843025E-4 3.97051495593721E-5 0.0 0.5187080738436372 0.0 54-55 0.0010323338885436747 3.97051495593721E-5 0.0 0.6038756196484902 0.0 56-57 0.0010323338885436747 3.97051495593721E-5 0.0 0.702979672948683 0.0 58-59 0.0010323338885436747 3.97051495593721E-5 0.0 0.8147695215330952 0.0 60-61 0.0010918916128827328 3.97051495593721E-5 0.0 0.9349768618240942 0.0 62-63 0.001151449337221791 3.97051495593721E-5 0.0 1.0594525056927258 0.0 64-65 0.001151449337221791 3.97051495593721E-5 0.0 1.1903008260656365 0.0 66-67 0.0011911544867811632 3.97051495593721E-5 0.0 1.3434038827665753 0.0 68-69 0.0012308596363405351 3.97051495593721E-5 0.0 1.5188013809451015 0.0 70-71 0.0012705647858999073 3.97051495593721E-5 0.0 1.705514846748049 0.0 72-73 0.0012705647858999073 5.9557724339058154E-5 0.0 1.9023729782634158 0.0 74-75 0.0012705647858999073 7.94102991187442E-5 0.0 2.110269141356288 0.0 76-77 0.0012705647858999073 7.94102991187442E-5 0.0 2.3336106076277563 0.0 78-79 0.0013102699354592795 7.94102991187442E-5 0.0 2.5898279377343845 0.0 80-81 0.0013499750850186514 7.94102991187442E-5 0.0 2.8721911088258594 0.0 82-83 0.0013698276597983376 7.94102991187442E-5 0.0 3.1504845020874983 0.0 84-85 0.0014095328093577096 7.94102991187442E-5 0.0 3.427189689366762 0.0 86-87 0.0014293853841373958 7.94102991187442E-5 0.0 3.7288495631440917 0.0 88-89 0.0014293853841373958 7.94102991187442E-5 0.0 4.0623132617184785 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 7180 0.0 27.428568 1 TACATGG 7405 0.0 26.21169 2 ACATGGG 7420 0.0 25.646019 3 GTATCAA 12320 0.0 23.727364 1 CAACGCA 13890 0.0 20.94336 5 CATGGGA 4655 0.0 20.89843 4 ATCAACG 13955 0.0 20.81139 3 TCAACGC 13935 0.0 20.807205 4 TATCAAC 14150 0.0 20.592484 2 AACGCAG 14320 0.0 20.475288 6 GAGTACT 5200 0.0 18.933628 12-13 AGAGTAC 8715 0.0 18.565475 10-11 ACGCAGA 15545 0.0 18.312391 7 GGTATCA 9380 0.0 18.010536 1 CGCAGAG 15700 0.0 17.860338 8 GAGTACA 5980 0.0 17.855042 1 GTCCTAG 1305 0.0 17.81825 1 CATGGGG 3855 0.0 17.603182 4 GTACTTT 5725 0.0 17.447899 14-15 AGTACTT 5465 0.0 17.234133 12-13 >>END_MODULE