FastQCFastQC Report
Fri 27 May 2016
SRR939593_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939593_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2518565
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56660.2249693774034023No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT49810.19777134995523243No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44200.17549676105242468No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30210.11994925681886312No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT25830.10255840131185814No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG68550.025.004691
TACATGG70150.023.1566522
ACATGGG69550.021.8657253
GGTATCA93050.021.0525421
GTATCAA120050.020.2402151
GAGTACT57800.018.71028712-13
AGAGTAC91600.018.12006210-11
CATGGGA42250.017.8300154
TCAACGC135200.017.760374
AACGCAG135800.017.6241056
ATCAACG136550.017.5847823
CAACGCA136250.017.5543885
AGTACTT60300.017.46671112-13
GTACTTT63000.017.46448514-15
TATCAAC143900.016.7530382
GAGTACA58650.016.7002471
CATGGGG36350.016.3201684
AGTACAT53500.016.2817972
ACGCAGA148450.015.9005667
CGCAGAG146850.015.8496738
Produced by FastQC (version 0.11.5)