FastQCFastQC Report
Fri 27 May 2016
SRR939592_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939592_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2392275
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG55250.033.9106861
GTATCAA102300.026.8808461
TACATGG67600.025.4335612
ACATGGG67250.024.5134163
TATCAAC122800.020.3859042
CATGGGA41900.019.7268754
ATCAACG128650.019.6800273
GAGTACT48100.019.6217212-13
TCAACGC129350.019.535374
CAACGCA128950.019.4701715
AACGCAG132850.018.9337226
GGTATCA79650.018.8432731
AGAGTAC82200.018.54273410-11
GTATAAG9850.018.4073891
GTATAGG6656.4355845E-918.176721
AGTACTT52650.018.10527812-13
ACGCAGA138250.017.9210957
GAGTACA50750.017.8633271
GTACTTT52750.017.76081814-15
CATGGGG36400.017.3875224
Produced by FastQC (version 0.11.5)