FastQCFastQC Report
Fri 27 May 2016
SRR939592_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939592_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2392275
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53520.22372009906887796No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT48790.20394812469302234No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41560.17372584673584768No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28850.1205965033284217No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG68100.023.3507021
GTATCAA107900.022.4343281
TACATGG70650.021.768662
ACATGGG69000.021.7390563
GGTATCA81000.020.9134941
GAGTACT51250.019.63704512-13
TCAACGC122900.019.4589214
ATCAACG123150.019.419423
CAACGCA123600.019.3105555
AACGCAG124350.019.1429396
AGAGTAC85350.018.88798510-11
CATGGGG37150.018.791684
TATCAAC128100.018.745412
AGTACTT54600.018.51854112-13
GTACTTT54950.017.75721214-15
CGCAGAG134750.017.2806978
ACGCAGA136600.017.2191967
GAGTACA58000.017.16611
AGTACAT52150.016.8261572
TACACTG19500.016.207015
Produced by FastQC (version 0.11.5)