##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939592_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2392275 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.489437042146076 31.0 31.0 34.0 30.0 34.0 2 31.703697108401 31.0 31.0 34.0 30.0 34.0 3 31.759603306476052 31.0 31.0 34.0 30.0 34.0 4 35.55018716493714 37.0 35.0 37.0 33.0 37.0 5 35.3000043891275 37.0 35.0 37.0 33.0 37.0 6 35.30085002769331 37.0 35.0 37.0 32.0 37.0 7 35.21182598154476 37.0 35.0 37.0 32.0 37.0 8 35.17416099737697 37.0 35.0 37.0 32.0 37.0 9 36.787210082452894 39.0 37.0 39.0 32.0 39.0 10-11 36.661097072869964 39.0 35.0 39.0 32.0 39.0 12-13 36.60144297791851 39.0 35.0 39.0 32.0 39.0 14-15 37.69794818739484 39.0 36.5 41.0 32.0 41.0 16-17 37.64353366565298 39.0 36.0 41.0 32.0 41.0 18-19 37.59409244338548 39.0 36.0 41.0 32.0 41.0 20-21 37.528556917578456 39.0 36.0 41.0 32.0 41.0 22-23 37.393546519526396 39.0 36.0 41.0 32.0 41.0 24-25 37.2613317866884 39.0 36.0 40.5 31.0 41.0 26-27 36.988134934319845 39.0 36.0 40.0 31.0 41.0 28-29 36.788051958909406 39.0 36.0 40.0 30.0 41.0 30-31 36.57105245007368 38.0 35.0 40.0 30.0 41.0 32-33 36.43332560010869 38.0 35.0 40.0 30.0 41.0 34-35 36.425806815688 38.0 35.0 40.0 30.0 41.0 36-37 36.36814308555664 38.0 35.0 40.0 30.0 41.0 38-39 36.43882663991389 38.5 35.0 40.0 30.0 41.0 40-41 36.353693241788676 38.5 35.0 40.0 30.0 41.0 42-43 36.193758451683024 38.0 35.0 40.0 30.0 41.0 44-45 36.01608448025415 38.0 35.0 40.0 29.0 41.0 46-47 35.77288731437648 38.0 35.0 40.0 28.5 41.0 48-49 35.5224021067812 38.0 34.0 40.0 27.5 41.0 50-51 35.2941423958366 38.0 34.0 40.0 27.0 41.0 52-53 35.08433394990125 38.0 34.0 40.0 26.5 41.0 54-55 34.80077165041645 38.0 33.5 40.0 26.0 41.0 56-57 33.93632003009687 37.5 32.5 40.0 23.5 41.0 58-59 30.635561756069016 35.5 26.5 39.0 8.5 40.5 60-61 30.35792979486054 34.5 26.0 39.0 10.5 40.0 62-63 32.3303399483755 35.5 30.5 39.0 20.5 40.0 64-65 32.424972254443986 36.0 31.0 39.0 19.5 40.0 66-67 27.298819324701384 31.5 18.5 37.0 4.5 39.5 68-69 19.586394958773553 19.5 8.0 31.5 2.0 37.0 70-71 17.648531000825574 15.5 7.5 29.0 2.0 35.0 72-73 19.345631041581758 19.5 7.5 30.5 2.0 35.5 74-75 20.823081167507915 22.5 9.0 32.0 2.0 36.0 76-77 22.73849264821143 27.5 11.5 33.0 2.0 35.5 78-79 24.99596743685404 30.5 13.0 34.5 2.0 36.5 80-81 26.657551702876965 32.0 22.5 35.0 2.0 36.5 82-83 27.875764282952417 33.0 26.5 35.0 2.0 36.0 84-85 27.95144621751262 34.0 27.5 35.0 2.0 36.0 86-87 27.681929335047183 33.5 27.0 35.0 2.0 36.0 88-89 27.24776959170664 33.0 26.0 35.0 2.0 35.0 90-91 25.33800294698561 32.5 16.0 34.5 2.0 35.0 92-93 23.60548766341662 31.0 2.0 34.0 2.0 35.0 94-95 19.681856391928186 27.0 2.0 34.0 2.0 35.0 96-97 16.965539496922386 17.0 2.0 33.0 2.0 35.0 98-99 17.223293517676687 20.0 2.0 33.0 2.0 35.0 100-101 16.430222486963245 9.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.23692667730808736 1101 2 0.1860843739270308 1101 3 0.21396880301261945 1101 4 0.23375645727478656 1101 5 0.30160446128205365 1101 6 0.2567453063500409 1101 7 0.3362788193452104 1101 8 0.42233067162882065 1101 9 0.41126566212357574 1101 10-11 0.35514863991074463 1101 12-13 0.37893877467380577 1101 14-15 0.5373134940641435 1101 16-17 0.41167450116037685 1101 18-19 0.43681714460912247 1101 20-21 0.4160888774219771 1101 22-23 0.4016029767310485 1101 24-25 0.40445580631960354 1101 26-27 0.44805310173479285 1101 28-29 0.4004781469186014 1101 30-31 0.2054143242303681 1101 32-33 0.420183587299789 1101 34-35 0.20498994833555884 1101 36-37 0.13379000183520873 1101 38-39 0.07171179988681331 1101 40-41 0.1454585590567774 1101 42-43 0.22229202665445058 1101 44-45 0.13329514323180547 1101 46-47 0.09276268678336663 1101 48-49 -0.09593485130081092 1101 50-51 -0.03918924648669275 1101 52-53 -0.13541694310762153 1101 54-55 -0.13064280781295423 1101 56-57 -3.687559184525618 1101 58-59 -10.780212808592427 1101 60-61 -12.339095902479187 1101 62-63 -4.308707245771451 1101 64-65 -1.5184608232499386 1101 66-67 -2.4990472438374987 1101 68-69 -4.882658446414254 1101 70-71 -4.538475555590438 1101 72-73 -3.5514838411371343 1101 74-75 -6.339550067100902 1101 76-77 -5.014602293512443 1101 78-79 1.2140024410792165 1101 80-81 1.79356928318008 1101 82-83 0.5312905385789186 1101 84-85 0.23515123717651676 1101 86-87 0.308820296855739 1101 88-89 0.13845355623534417 1101 90-91 1.5884163459501899 1101 92-93 1.001527905844359 1101 94-95 -8.561801076792179 1101 96-97 -9.763204517632124 1101 98-99 -8.828748491157219 1101 100-101 -9.74291273544516 1102 1 0.24762853203219137 1102 2 0.30656575503303074 1102 3 0.30153418543271115 1102 4 0.21425504373124227 1102 5 0.33138875780631594 1102 6 0.2666671160544922 1102 7 0.32578839484430944 1102 8 0.30106521277411247 1102 9 0.2819580773811765 1102 10-11 0.26681646286645133 1102 12-13 0.2801721992572439 1102 14-15 0.4095456196767415 1102 16-17 0.30607345818142306 1102 18-19 0.2921447901621974 1102 20-21 0.33450475231749977 1102 22-23 0.2787074311827453 1102 24-25 0.32616404442172353 1102 26-27 0.10371219440821022 1102 28-29 0.20985090056250044 1102 30-31 0.26597028777968035 1102 32-33 0.026452767067183913 1102 34-35 0.066824762280568 1102 36-37 -0.04321238136363803 1102 38-39 0.16017979348456635 1102 40-41 0.039719107055688596 1102 42-43 0.04050192991360291 1102 44-45 -0.12054246654664524 1102 46-47 -0.01780257364667648 1102 48-49 0.17619629765682276 1102 50-51 0.16004737230620236 1102 52-53 -0.00841377496686846 1102 54-55 -0.2299415594384726 1102 56-57 -3.730336501980176 1102 58-59 -10.322246879679934 1102 60-61 -8.196696134879605 1102 62-63 -2.5484914429177437 1102 64-65 -0.9056146944753962 1102 66-67 -1.9534345577936598 1102 68-69 -5.161373529641862 1102 70-71 -4.828950364788344 1102 72-73 -3.6322343435359628 1102 74-75 -4.285483988819006 1102 76-77 -4.364877289627341 1102 78-79 1.5072939674945296 1102 80-81 1.7879032988607726 1102 82-83 0.3515980370497225 1102 84-85 0.0723496772593073 1102 86-87 -0.06656275839452164 1102 88-89 0.02082000197087197 1102 90-91 1.4016712494246626 1102 92-93 0.7665771505070076 1102 94-95 -9.374412615956675 1102 96-97 -10.099907292106504 1102 98-99 -9.101913098947673 1102 100-101 -9.979248013081992 1103 1 0.20893112638566436 1103 2 0.1693207100415428 1103 3 0.18407629257688995 1103 4 0.17105813009276005 1103 5 0.15033668909663334 1103 6 0.2356869065091729 1103 7 0.23977895558414986 1103 8 0.28245908097396466 1103 9 0.31393249784481014 1103 10-11 0.26520165986422484 1103 12-13 0.20870934080321035 1103 14-15 0.3814710073350014 1103 16-17 0.34394795470419837 1103 18-19 0.3541905011773565 1103 20-21 0.3868350095415778 1103 22-23 0.2198137880588007 1103 24-25 0.40520955599162534 1103 26-27 0.3761521192421853 1103 28-29 0.3905687656453196 1103 30-31 0.42787123226569435 1103 32-33 0.2131697846871461 1103 34-35 0.24527707856656633 1103 36-37 0.22278929546985893 1103 38-39 0.22577423727567947 1103 40-41 0.23394095250012015 1103 42-43 0.1720815234329649 1103 44-45 0.23539724728315292 1103 46-47 0.174483618954838 1103 48-49 0.021230706630454677 1103 50-51 -0.09747924516397433 1103 52-53 0.08653107030654184 1103 54-55 0.019149126697726615 1103 56-57 0.3105510681864345 1103 58-59 -8.016658765296285 1103 60-61 -7.623995969163424 1103 62-63 -2.1899536279389054 1103 64-65 -0.5285665934196864 1103 66-67 -1.8273968573271269 1103 68-69 -3.477611432080824 1103 70-71 -3.978279365751396 1103 72-73 -3.159880270624001 1103 74-75 -3.891682916868948 1103 76-77 -0.6603680032177728 1103 78-79 3.3289899069527706 1103 80-81 2.794779278228166 1103 82-83 1.1670337071951202 1103 84-85 0.6968605230464853 1103 86-87 0.6515995538609403 1103 88-89 0.45404697885890855 1103 90-91 1.9130418547546562 1103 92-93 1.5503194758491752 1103 94-95 -7.459565474449475 1103 96-97 -9.009881809349185 1103 98-99 -8.236074424458849 1103 100-101 -9.828467398127216 1104 1 0.25651768188976476 1104 2 0.2693981537003083 1104 3 0.338272627602219 1104 4 0.24037604214945674 1104 5 0.38855874527550327 1104 6 0.27455878027816993 1104 7 0.3088681256224888 1104 8 0.25691434850483574 1104 9 0.31532613777892493 1104 10-11 0.4106227765144368 1104 12-13 0.3164249950161988 1104 14-15 0.5198337679614511 1104 16-17 0.37265940205310244 1104 18-19 0.4056135662425717 1104 20-21 0.41707306123035437 1104 22-23 0.4768236066694129 1104 24-25 0.3854979611927618 1104 26-27 0.4070418046202988 1104 28-29 0.3870202375288585 1104 30-31 0.4024514647177426 1104 32-33 0.5122687832813995 1104 34-35 0.34626105840958843 1104 36-37 0.312780037687979 1104 38-39 0.34820007881064896 1104 40-41 0.3231313138562939 1104 42-43 0.32440100641546366 1104 44-45 0.3923927533024383 1104 46-47 0.17932655015624022 1104 48-49 0.18347084769812483 1104 50-51 0.2528849908268782 1104 52-53 0.2904930636743259 1104 54-55 0.20254812066525574 1104 56-57 0.7062090344407181 1104 58-59 -8.38293116547461 1104 60-61 -7.5911180875653095 1104 62-63 -2.1293882330173233 1104 64-65 -0.3628782420961585 1104 66-67 -1.5884959259709213 1104 68-69 -3.2257210673951313 1104 70-71 -3.8170542944154526 1104 72-73 -2.873304573231099 1104 74-75 -3.6927878402706398 1104 76-77 -0.23470721336164146 1104 78-79 3.7211025881248823 1104 80-81 3.1868314227602603 1104 82-83 1.4279985142194462 1104 84-85 1.0973735278301753 1104 86-87 1.037004342914532 1104 88-89 1.0658784759862314 1104 90-91 2.5836213503893184 1104 92-93 2.017174368225838 1104 94-95 -6.374648351271681 1104 96-97 -8.17098559120281 1104 98-99 -5.997564386835325 1104 100-101 -5.171182181044987 1105 1 0.19148180234757461 1105 2 0.24799648847725564 1105 3 0.17825405679861817 1105 4 0.1278758298531315 1105 5 0.2766368574370617 1105 6 0.27396039747833356 1105 7 0.24284250599541934 1105 8 0.2162331027910227 1105 9 0.20590305083231186 1105 10-11 0.1668915208153834 1105 12-13 0.1661371358474213 1105 14-15 0.3430034515784328 1105 16-17 0.3529125500414949 1105 18-19 0.33920317783693577 1105 20-21 0.28089053558934296 1105 22-23 0.42887941102648597 1105 24-25 0.3404105419605301 1105 26-27 0.3568982191023622 1105 28-29 0.40229942329487756 1105 30-31 0.41489898360332944 1105 32-33 0.3121109812305889 1105 34-35 0.3799549733821337 1105 36-37 0.35577538649522467 1105 38-39 0.3869964992311523 1105 40-41 0.2661092543653041 1105 42-43 0.21171653555452963 1105 44-45 0.2739669846872772 1105 46-47 0.1720704083504998 1105 48-49 0.12847943911057058 1105 50-51 0.19140368373746952 1105 52-53 0.2705375024184846 1105 54-55 0.2505906314660109 1105 56-57 0.981327935021028 1105 58-59 -8.69049480019844 1105 60-61 -7.9563296898959415 1105 62-63 -2.2539142265787433 1105 64-65 -0.12576946029051328 1105 66-67 -1.6566545379687945 1105 68-69 -3.5368907381279335 1105 70-71 -3.9824197310518805 1105 72-73 -2.9157092990350257 1105 74-75 -3.4887867427718717 1105 76-77 -0.0198662657645734 1105 78-79 3.936372227877058 1105 80-81 3.3232883230934362 1105 82-83 1.7400007525547174 1105 84-85 1.3416177353616874 1105 86-87 1.1342009919708502 1105 88-89 1.154571852165354 1105 90-91 2.441616634775613 1105 92-93 1.9326772118252364 1105 94-95 -6.643399899570705 1105 96-97 -7.955755336078269 1105 98-99 -5.71114400146919 1105 100-101 -5.1802898395834855 1106 1 0.10904818319517062 1106 2 0.18113849635872015 1106 3 0.2472734524175415 1106 4 0.11249064273547305 1106 5 0.24517621312927673 1106 6 0.1595682440804751 1106 7 0.23314857696921365 1106 8 0.21002858226082566 1106 9 0.13618054984876693 1106 10-11 0.08127848039734431 1106 12-13 0.09953935689025428 1106 14-15 4.05251146098351E-4 1106 16-17 0.18078314820790098 1106 18-19 0.07060976467008118 1106 20-21 0.16070518800068356 1106 22-23 0.11848346614301875 1106 24-25 0.2221908596668456 1106 26-27 0.1642938398200755 1106 28-29 0.23805514440248032 1106 30-31 0.3506242432791993 1106 32-33 0.13013026879426803 1106 34-35 0.132179807105409 1106 36-37 0.14729375081335405 1106 38-39 0.2787726738245553 1106 40-41 0.14101515376407292 1106 42-43 0.2096731122686819 1106 44-45 0.21758050150359765 1106 46-47 0.18866017271244573 1106 48-49 0.16373447935070828 1106 50-51 0.10756176438966492 1106 52-53 0.23162626950807663 1106 54-55 0.09484809190495014 1106 56-57 0.6965553375937006 1106 58-59 -8.510143871371032 1106 60-61 -7.8616883646729185 1106 62-63 -2.0229341893384856 1106 64-65 -0.3854141376142515 1106 66-67 0.5538964154546164 1106 68-69 -2.4117765637267574 1106 70-71 -2.487690633325162 1106 72-73 -1.0710311842827416 1106 74-75 -0.9227170763879791 1106 76-77 2.285012219838112 1106 78-79 4.692758084220888 1106 80-81 3.75515605510925 1106 82-83 1.8667309051633545 1106 84-85 1.4913835808283515 1106 86-87 1.404537691533676 1106 88-89 1.361111776789354 1106 90-91 2.8700030176649136 1106 92-93 2.224898606884185 1106 94-95 -6.512235675887979 1106 96-97 -7.874042135010132 1106 98-99 -5.869154955047819 1106 100-101 -5.417207244179954 1107 1 0.05812984572611413 1107 2 0.017041054985917015 1107 3 0.06427600483376139 1107 4 0.09487379501386073 1107 5 0.07431619970173386 1107 6 0.021286289337801634 1107 7 0.017969242371989935 1107 8 0.13242477759312976 1107 9 0.04976795161919512 1107 10-11 0.06477228864374496 1107 12-13 0.08500056637470976 1107 14-15 0.08281014184767344 1107 16-17 0.13292231256517084 1107 18-19 0.042899116391851067 1107 20-21 0.09666053994131829 1107 22-23 0.17647906522143586 1107 24-25 0.10796779386893007 1107 26-27 0.06047800714973306 1107 28-29 0.02598765768954081 1107 30-31 0.012925307131013142 1107 32-33 0.11379325225236414 1107 34-35 0.08473067057923345 1107 36-37 0.09842644096540454 1107 38-39 0.19624119747507507 1107 40-41 0.09992169629004621 1107 42-43 -0.029108456506072855 1107 44-45 -0.10681407601679638 1107 46-47 0.10741044998825089 1107 48-49 -0.004531711338145783 1107 50-51 0.02740887081329646 1107 52-53 -0.028093097800287126 1107 54-55 0.12190910145507416 1107 56-57 0.39334965957671386 1107 58-59 -8.351821974124267 1107 60-61 -7.651952138995554 1107 62-63 -2.293744441001401 1107 64-65 -0.4572089032385591 1107 66-67 0.6277762951390962 1107 68-69 -2.503673751336745 1107 70-71 -2.559760678369539 1107 72-73 -0.9629546206690165 1107 74-75 -1.0601002138783322 1107 76-77 2.0959634311267124 1107 78-79 4.338215345297872 1107 80-81 3.144799397088658 1107 82-83 1.5223882188857694 1107 84-85 1.1368570348123974 1107 86-87 0.8639405207820587 1107 88-89 0.8739507557045627 1107 90-91 2.438996632187955 1107 92-93 1.8537239778422432 1107 94-95 -6.626131112593676 1107 96-97 -8.21523733421986 1107 98-99 -5.8697997137217435 1107 100-101 -5.139383689193574 1108 1 -0.08679412703164857 1108 2 -0.10701415257888769 1108 3 -0.07201263708130767 1108 4 -0.14308530047799906 1108 5 -0.09440152215584163 1108 6 -0.1363528832835783 1108 7 -0.08232704782779621 1108 8 -0.11468683386695488 1108 9 -0.10638153971990505 1108 10-11 -0.13974518160230787 1108 12-13 -0.17320598140354804 1108 14-15 -0.21804786518922015 1108 16-17 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3.2758218098253487 2313 60-61 3.28943120065399 2313 62-63 0.5094099030454231 2313 64-65 -0.28653355028287564 2313 66-67 -2.152634138032422 2313 68-69 -5.1344520283956765 2313 70-71 -3.8967103412043755 2313 72-73 -3.1070115206744298 2313 74-75 -3.9554797577880016 2313 76-77 0.13300212240854137 2313 78-79 2.846011049073759 2313 80-81 2.1064702444830736 2313 82-83 0.6713180728911503 2313 84-85 0.24192368583893042 2313 86-87 0.24458615748584123 2313 88-89 0.3576218557472721 2313 90-91 2.139149725024172 2313 92-93 3.701470445277682 2313 94-95 5.893716516422376 2313 96-97 1.269272317546573 2313 98-99 2.9042244322367843 2313 100-101 4.1714290563113945 2314 1 -0.3655622410398145 2314 2 -0.3688957600696341 2314 3 -0.40395390976075163 2314 4 -0.30154597673430317 2314 5 -0.3599359354354448 2314 6 -0.4003228105034822 2314 7 -0.4232808521031046 2314 8 -0.506406754415039 2314 9 -0.4882677221771985 2314 10-11 -0.4755553509904402 2314 12-13 -0.5217997510616073 2314 14-15 -0.7084543128420719 2314 16-17 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2314 82-83 0.8685532857741372 2314 84-85 0.5613468692920272 2314 86-87 0.5233487287784335 2314 88-89 0.6882127162517655 2314 90-91 2.153768002454754 2314 92-93 3.7852412478898607 2314 94-95 6.400876672154265 2314 96-97 3.5280209229035613 2314 98-99 5.845326882907383 2314 100-101 6.300809795803781 2315 1 -0.4559760765410097 2315 2 -0.46586382452313657 2315 3 -0.48493818465882654 2315 4 -0.35842473813985265 2315 5 -0.5494140303037511 2315 6 -0.5061166693011288 2315 7 -0.6534528251827609 2315 8 -0.7383979535349496 2315 9 -0.6623746916976785 2315 10-11 -0.6494253115159054 2315 12-13 -0.7084285610014263 2315 14-15 -0.7942759979517575 2315 16-17 -0.7574596162877896 2315 18-19 -0.7322009808989804 2315 20-21 -0.8243480416083528 2315 22-23 -0.7336893851103525 2315 24-25 -0.6260927260133258 2315 26-27 -0.6017772066406124 2315 28-29 -0.7721516552875585 2315 30-31 -0.6563613515528104 2315 32-33 -0.8453358701345053 2315 34-35 -0.7167536381569803 2315 36-37 -0.5042867880921236 2315 38-39 -0.7751549882256725 2315 40-41 -0.6813838093727753 2315 42-43 -0.6124545748516326 2315 44-45 -0.7123200506712948 2315 46-47 -0.6887250461949534 2315 48-49 -0.7140041580776568 2315 50-51 -0.644982674863499 2315 52-53 -0.507461518196429 2315 54-55 -0.5630744339438252 2315 56-57 0.12907534934372222 2315 58-59 3.3260191050554972 2315 60-61 3.410850335683886 2315 62-63 0.6491795224771195 2315 64-65 0.005612593044432401 2315 66-67 -1.7347608125793066 2315 68-69 -5.0091507258415575 2315 70-71 -3.730007460288414 2315 72-73 -3.1456274989806445 2315 74-75 -0.5691838091485018 2315 76-77 2.143462246174362 2315 78-79 3.904736075727449 2315 80-81 2.7154939662649333 2315 82-83 0.834864519838721 2315 84-85 0.5625125005610379 2315 86-87 0.6299492270635518 2315 88-89 0.5386887565028466 2315 90-91 2.163361123531569 2315 92-93 3.6475619137799775 2315 94-95 6.219512940192832 2315 96-97 3.7391101935953373 2315 98-99 6.390509371744503 2315 100-101 6.653966545302259 2316 1 -0.4488650556374587 2316 2 -0.4398579077273119 2316 3 -0.5259559719494042 2316 4 -0.2846539186451835 2316 5 -0.48109308000811524 2316 6 -0.5693537060648097 2316 7 -0.359251633622911 2316 8 -0.5128298509279503 2316 9 -0.6123187220074158 2316 10-11 -0.5640658958968814 2316 12-13 -0.5207363573947603 2316 14-15 -0.525124830583934 2316 16-17 -0.749263029413811 2316 18-19 -0.6823810372376968 2316 20-21 -0.6375025589569034 2316 22-23 -0.8377327851972751 2316 24-25 -0.6011933894489232 2316 26-27 -0.6120949525177153 2316 28-29 -0.654091743116922 2316 30-31 -0.6079111789283544 2316 32-33 -0.5872868956304416 2316 34-35 -0.5204097095357625 2316 36-37 -0.3778416732414982 2316 38-39 -0.554987806965876 2316 40-41 -0.6474670483982123 2316 42-43 -0.6125880922903875 2316 44-45 -0.5217076336007409 2316 46-47 -0.7134662632589439 2316 48-49 -0.6266127659289964 2316 50-51 -0.6185413528307109 2316 52-53 -0.5286574756676714 2316 54-55 -0.5745635967356648 2316 56-57 0.1566491350269601 2316 58-59 3.4050601474448463 2316 60-61 3.504013217733398 2316 62-63 0.9092725182701642 2316 64-65 0.23469904673925157 2316 66-67 -2.148608749286403 2316 68-69 -3.303138186990804 2316 70-71 -3.347785986378902 2316 72-73 -0.5414331549946176 2316 74-75 5.8124203067764135 2316 76-77 5.840240554946636 2316 78-79 4.822543663003259 2316 80-81 3.323243051888305 2316 82-83 1.4171527780947635 2316 84-85 1.0859187995088924 2316 86-87 0.852561494471562 2316 88-89 0.9247184245390265 2316 90-91 2.499441174539843 2316 92-93 3.965252179974282 2316 94-95 6.771130884526567 2316 96-97 3.7693210127619814 2316 98-99 6.251301681140031 2316 100-101 6.501943999562927 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 13.0 4 26.0 5 96.0 6 195.0 7 472.0 8 1031.0 9 1847.0 10 3024.0 11 4666.0 12 6717.0 13 9341.0 14 11541.0 15 15734.0 16 21238.0 17 28474.0 18 32355.0 19 33661.0 20 36498.0 21 37700.0 22 37977.0 23 38466.0 24 40531.0 25 42573.0 26 52868.0 27 75133.0 28 107834.0 29 143285.0 30 174300.0 31 191891.0 32 198687.0 33 196642.0 34 199693.0 35 212125.0 36 212166.0 37 159072.0 38 60674.0 39 3722.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.933542979305926 19.74895166560673 14.222894561121166 24.094610793966176 2 15.637971738046193 21.198012189906347 39.01827733437459 24.145738737672865 3 19.153406243194222 24.794034450139176 30.15718550046002 25.895373806206578 4 13.008705102883239 17.28576355143117 37.394404907462565 32.311126438223035 5 13.531171047568055 37.60733347834089 34.9151705790821 13.946324895008951 6 31.912969871774777 37.02157987689542 17.36547846714947 13.699971784180331 7 28.017013094230386 32.563689375176345 21.479261372542872 17.94003615805039 8 25.650573471099047 35.60378117222358 20.05439176281647 18.691253593860903 9 25.854260066254923 16.573638064185765 20.309036377506768 37.26306549205255 10-11 24.637489419067624 26.507968356480756 28.16599262208567 20.68854960236595 12-13 25.646926043201557 23.888328055930025 28.193518721718863 22.271227179149555 14-15 22.420488532833946 25.369346623388296 26.248199927683597 25.96196491609416 16-17 22.280390958560766 28.53180697528795 26.75216133269015 22.435640733461135 18-19 22.147095805479662 27.64797279898874 28.443892480094167 21.761038915437428 20-21 22.852514865556845 26.746757793313897 28.28713254120032 22.113594799928936 22-23 22.55562174081157 26.790251956819343 28.112069055606064 22.54205724676302 24-25 22.68160866255649 26.843278338216415 28.151427533563268 22.323685465663825 26-27 22.574409358798096 26.979111497726223 28.144802035553635 22.301677107922046 28-29 22.31693545471095 27.209984907516997 28.166803601880318 22.306276035891727 30-31 22.594518489844496 26.84109406136987 28.075945038586607 22.48844241019902 32-33 22.379329307789405 26.966894294043346 28.154847091966378 22.498929306200868 34-35 22.886732092343927 26.90049581332238 27.94535728364025 22.26741481069345 36-37 22.693903867178168 26.765384050552093 28.06878739661996 22.471924685649782 38-39 22.442103043623508 27.056204566909997 28.229842826688902 22.2718495627776 40-41 22.706062162985376 26.87841198541988 28.084405532144206 22.331120319450534 42-43 22.420582121625966 27.04758043460797 27.848453734200945 22.683383709565124 44-45 22.70388491087011 26.897590273224548 27.76805182056948 22.630472995335865 46-47 22.70724087333482 27.12765846170196 27.619780616110827 22.54532004885239 48-49 22.77896799548512 27.170975924133977 27.50237520598715 22.547680874393752 50-51 22.428925813441765 27.180396241727532 27.758527647398406 22.632150297432293 52-53 22.832069287449556 27.275366247046932 27.286136988776676 22.606427476726832 54-55 22.559888081354316 26.948993579106844 27.494408772815817 22.996709566723023 56-57 22.532665456490225 27.18661585849846 27.61509277845091 22.665625906560408 58-59 21.76591972380147 26.754290389540063 29.06996696793243 22.40982291872604 60-61 21.96652219486957 26.897927802131385 28.734437963044137 22.401112039954917 62-63 22.711727946847454 27.017915240683404 27.6676703415128 22.602686470956343 64-65 22.536000503297455 27.119121603813355 27.514465099101315 22.830412793787872 66-67 22.291699868404866 26.775403504159005 28.27462843291882 22.658268194517305 68-69 21.575652146355555 26.176923345408078 29.9029202717984 22.34450423643797 70-71 22.458751080260058 26.17640801920006 28.91229636643106 22.452544534108824 72-73 21.81395472618015 26.296456762064945 29.257043096886825 22.63254541486808 74-75 21.91563735292947 26.650607593671573 28.694073812445954 22.739681240953008 76-77 20.865155434059105 26.948403291943634 28.89739092641348 23.289050347583782 78-79 22.022153881595553 26.936289146010512 27.9755865477565 23.065970424637435 80-81 22.117734483727652 27.070634501124076 27.608397287495983 23.203233727652286 82-83 22.46640432191657 27.046715423664196 27.38971571794512 23.097164536474114 84-85 22.359624536784484 27.04984230294138 27.212785479748103 23.377747680526035 86-87 22.295161596082007 26.979275329392404 27.459054683141844 23.266508391383745 88-89 22.317902899520938 26.942769318487493 27.197610474884886 23.541717307106687 90-91 22.091538246348076 26.80025064033657 27.71301597580499 23.395195137510363 92-93 21.987409846268125 27.011906733653547 27.61345442499862 23.38722899507971 94-95 20.890096708076005 26.824335047560517 28.488657736477247 23.79691050788623 96-97 20.582643705057798 26.827289109983678 28.66677250991537 23.92329467504316 98-99 21.28133098806518 26.690910360813234 27.966312962024453 24.061445689097134 100-101 20.474197238183592 26.741292512587776 28.48862375249564 24.29588649673299 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 240.0 1 177.0 2 119.0 3 142.0 4 175.5 5 222.0 6 276.5 7 410.0 8 623.5 9 792.5 10 872.5 11 950.0 12 1077.0 13 1223.0 14 1348.0 15 1479.0 16 1670.0 17 1873.5 18 2083.5 19 2348.0 20 2787.5 21 3327.5 22 3929.0 23 4811.5 24 6134.0 25 7946.0 26 10292.5 27 13314.5 28 16816.0 29 20778.5 30 25422.5 31 30574.0 32 36029.5 33 41641.0 34 47638.0 35 53630.0 36 59475.0 37 65072.5 38 69618.5 39 73113.5 40 76285.0 41 79610.5 42 82955.0 43 86619.0 44 89822.0 45 92362.0 46 95683.0 47 100003.0 48 103558.5 49 102602.0 50 98295.0 51 94276.5 52 90209.5 53 85495.0 54 80067.5 55 73707.0 56 66236.5 57 58075.5 58 50134.0 59 42285.5 60 34581.0 61 27017.5 62 20498.0 63 15694.5 64 11526.0 65 8062.0 66 5585.0 67 3876.0 68 2517.0 69 1554.5 70 993.0 71 634.5 72 417.5 73 287.0 74 185.0 75 98.5 76 56.0 77 36.5 78 15.5 79 9.0 80 5.0 81 3.0 82 3.0 83 1.0 84 0.0 85 0.5 86 0.5 87 0.5 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02608395773897232 2 0.012373159440281742 3 2.508072859516569E-4 4 0.0 5 0.06667293684881546 6 0.0 7 0.0 8 4.180121432527615E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 4.180121432527615E-4 16-17 4.180121432527615E-5 18-19 4.598133575780376E-4 20-21 0.0 22-23 0.0 24-25 8.36024286505523E-5 26-27 8.36024286505523E-5 28-29 0.0010241297509692656 30-31 0.005183350576334242 32-33 0.005622263326749643 34-35 0.0018392534303121504 36-37 0.09137745451505366 38-39 0.08425034747259408 40-41 0.09075043630017451 42-43 0.09442894316079882 44-45 0.09764763666384509 46-47 0.0927150933734625 48-49 0.08176317522024015 50-51 0.07586920400037621 52-53 0.0643320688466 54-55 0.052356020942408384 56-57 0.07166818196068596 58-59 0.7299119039408095 60-61 0.3422683428953611 62-63 0.002758880145468226 64-65 0.0021527625377517215 66-67 1.1155072054843194 68-69 2.392513402514343 70-71 2.342205641073873 72-73 1.8303706722680293 74-75 1.2955450355832838 76-77 0.7445423289546562 78-79 0.5645044988556918 80-81 0.04092338882444535 82-83 1.2540364297582846E-4 84-85 0.0012540364297582846 86-87 0.0012331358225956463 88-89 2.0900607162638075E-5 90-91 0.33365729274435424 92-93 0.05680785026805029 94-95 0.9648974302703494 96-97 1.6611593566793117 98-99 0.6510330125090134 100-101 0.5800545505846945 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2392275.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.13181000573749 #Duplication Level Percentage of deduplicated Percentage of total 1 76.05307558323817 43.45049864573557 2 13.438290031128822 15.355076657208963 3 4.564900961554189 7.824031632915669 4 2.0547718086256177 4.695713303021776 5 1.0834156204162249 3.0948747691433987 6 0.6419782942507736 2.2006429160965584 7 0.43599707664466003 1.7436511502143783 8 0.3088333499601412 1.4115366618686582 9 0.2205793125974076 1.1341885840660144 >10 1.094897870637472 11.083014578400267 >50 0.06598948208169027 2.594356334586655 >100 0.03441398100480942 3.4386049798703064 >500 0.0019776654415586585 0.7337498845177141 >1k 8.057155502629393E-4 1.0160933583862448 >5k 7.324686820572176E-5 0.2239665439678425 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5352 0.22372009906887796 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4879 0.20394812469302234 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4156 0.17372584673584768 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2885 0.1205965033284217 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 4.180121432527615E-5 0.0 0.0 0.0 0.0 7 4.180121432527615E-5 0.0 0.0 0.0 0.0 8 1.2540364297582846E-4 0.0 0.0 0.0 0.0 9 1.672048573011046E-4 0.0 0.0 4.180121432527615E-5 0.0 10-11 2.508072859516569E-4 0.0 0.0 6.270182148791423E-5 0.0 12-13 2.92608500276933E-4 0.0 0.0 8.36024286505523E-5 0.0 14-15 3.344097146022092E-4 0.0 0.0 8.36024286505523E-5 0.0 16-17 4.180121432527615E-4 0.0 0.0 8.36024286505523E-5 0.0 18-19 5.434157862285899E-4 0.0 0.0 1.672048573011046E-4 0.0 20-21 5.85217000553866E-4 0.0 0.0 3.5531032176484725E-4 0.0 22-23 6.479188220417803E-4 0.0 0.0 5.434157862285899E-4 0.0 24-25 7.733224650176087E-4 0.0 0.0 7.524218578549707E-4 0.0 26-27 7.942230721802468E-4 0.0 0.0 0.0012122352154330083 0.0 28-29 7.942230721802468E-4 0.0 0.0 0.0026752777168176736 0.0 30-31 8.987261079934372E-4 0.0 0.0 0.007628721614362897 0.0 32-33 9.614279294813514E-4 0.0 0.0 0.018204428838657763 0.0 34-35 0.0010659309652945419 0.0 0.0 0.034088890282262696 0.0 36-37 0.001128632786782456 0.0 0.0 0.06023554984272293 0.0 38-39 0.001170434001107732 0.0 0.0 0.09635179901976151 0.0 40-41 0.0012122352154330083 0.0 0.0 0.1372751878442069 0.0 42-43 0.0012331358225956463 0.0 0.0 0.18010053192045228 0.0 44-45 0.001379440072734113 0.0 0.0 0.23488102329372668 0.0 46-47 0.0015675455371978557 0.0 0.0 0.2971439320312255 0.0 48-49 0.0015884461443604937 0.0 0.0 0.35836181041059245 0.0 50-51 0.0015884461443604937 0.0 0.0 0.4214607434345968 0.0 52-53 0.00163024735868577 0.0 0.0 0.4852493964949682 0.0 54-55 0.00163024735868577 0.0 0.0 0.5567921748126783 0.0 56-57 0.00163024735868577 0.0 0.0 0.6283349531303885 0.0 58-59 0.00163024735868577 0.0 0.0 0.6791652297499242 0.0 60-61 0.001672048573011046 0.0 0.0 0.7213635556112905 0.0 62-63 0.0017138497873363222 0.0 0.0 0.7599460764335204 0.0 64-65 0.0017556510016615984 4.180121432527615E-5 0.0 0.8037537490464097 0.0 66-67 0.0017556510016615984 4.180121432527615E-5 0.0 0.8417719534752485 4.180121432527615E-5 68-69 0.0017556510016615984 4.180121432527615E-5 0.0 0.887878692876028 4.180121432527615E-5 70-71 0.0017556510016615984 4.180121432527615E-5 0.0 0.9633716859474768 4.180121432527615E-5 72-73 0.0017556510016615984 4.180121432527615E-5 0.0 1.0646142270432957 4.180121432527615E-5 74-75 0.0017556510016615984 4.180121432527615E-5 0.0 1.196726964918331 4.180121432527615E-5 76-77 0.0017556510016615984 4.180121432527615E-5 0.0 1.3754271561588864 4.180121432527615E-5 78-79 0.0017765516088242364 4.180121432527615E-5 0.0 1.6006729995506368 6.270182148791423E-5 80-81 0.0018810546446374266 4.180121432527615E-5 0.0 1.8748262637029605 1.0450303581319037E-4 82-83 0.0019228558589627028 4.180121432527615E-5 0.0 2.1440051833505764 1.2540364297582846E-4 84-85 0.001964657073287979 4.180121432527615E-5 0.0 2.38289912321953 1.2540364297582846E-4 86-87 0.0020064582876132553 4.180121432527615E-5 0.0 2.6051143785726976 1.2540364297582846E-4 88-89 0.0020064582876132553 4.180121432527615E-5 0.0 2.826619013282336 1.2540364297582846E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 6810 0.0 23.350702 1 GTATCAA 10790 0.0 22.434328 1 TACATGG 7065 0.0 21.76866 2 ACATGGG 6900 0.0 21.739056 3 GGTATCA 8100 0.0 20.913494 1 GAGTACT 5125 0.0 19.637045 12-13 TCAACGC 12290 0.0 19.458921 4 ATCAACG 12315 0.0 19.41942 3 CAACGCA 12360 0.0 19.310555 5 AACGCAG 12435 0.0 19.142939 6 AGAGTAC 8535 0.0 18.887985 10-11 CATGGGG 3715 0.0 18.79168 4 TATCAAC 12810 0.0 18.74541 2 AGTACTT 5460 0.0 18.518541 12-13 GTACTTT 5495 0.0 17.757212 14-15 CGCAGAG 13475 0.0 17.280697 8 ACGCAGA 13660 0.0 17.219196 7 GAGTACA 5800 0.0 17.1661 1 AGTACAT 5215 0.0 16.826157 2 TACACTG 1950 0.0 16.20701 5 >>END_MODULE