FastQCFastQC Report
Fri 27 May 2016
SRR939591_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939591_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2769116
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80130.2893703261257383No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56330.20342231961391288No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39630.14311426462452279No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT37640.13592785567668528No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG92900.027.1261352
GTACATG95750.026.9630641
GTATCAA147850.026.1283631
ACATGGG95550.025.1817783
ATCAACG167950.022.7470443
TATCAAC167850.022.7323232
CAACGCA168600.022.630795
TCAACGC168750.022.6106744
AACGCAG170200.022.4434956
GGTATCA105950.021.3661631
GAGTACT71200.020.5958512-13
CATGGGA59900.020.2029424
ACGCAGA187850.019.804287
AGTACTT76350.019.51739712-13
CGCAGAG187400.019.4734238
AGAGTAC124250.019.2307610-11
GTACTTT79250.018.62354714-15
GCAGAGT188450.017.980249
CATGGGG46500.017.86024
TACACCG3501.3296813E-917.6269255
Produced by FastQC (version 0.11.5)