##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939591_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2769116 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.023917019005342 31.0 30.0 33.0 26.0 34.0 2 30.17221019271132 31.0 30.0 34.0 25.0 34.0 3 30.212253296719965 31.0 30.0 34.0 26.0 34.0 4 33.78066574314691 35.0 35.0 37.0 28.0 37.0 5 33.78394476793316 35.0 35.0 37.0 28.0 37.0 6 33.87133980663865 35.0 35.0 37.0 28.0 37.0 7 33.68272726747453 35.0 33.0 37.0 28.0 37.0 8 33.67894880532271 35.0 33.0 37.0 28.0 37.0 9 34.93216318854104 37.0 34.0 39.0 28.0 39.0 10-11 34.819356971683376 37.0 34.0 39.0 27.0 39.0 12-13 34.66215355369728 37.0 34.0 39.0 27.0 39.0 14-15 35.33320218437942 38.0 34.0 40.0 27.0 41.0 16-17 35.06023727427815 38.0 33.0 40.0 26.0 41.0 18-19 35.02189326846546 38.0 33.5 40.0 26.0 41.0 20-21 35.154457234727616 38.0 34.0 40.0 26.0 41.0 22-23 35.00612957348121 38.0 34.0 40.0 25.0 41.0 24-25 34.88440805657835 38.0 33.0 40.0 25.0 41.0 26-27 35.00957363288501 38.0 34.0 40.0 25.0 41.0 28-29 34.88867638625467 38.0 34.0 40.0 25.0 41.0 30-31 34.66919370658361 38.0 33.0 40.0 24.0 41.0 32-33 34.442063640526435 38.0 33.0 40.0 24.0 41.0 34-35 34.214652257254656 38.0 33.0 40.0 23.5 41.0 36-37 33.93235277973187 38.0 33.0 40.0 22.5 41.0 38-39 33.77636635662789 38.0 32.5 40.0 21.5 41.0 40-41 33.514522685217955 38.0 32.0 40.0 19.0 41.0 42-43 33.204464348911344 37.0 31.0 40.0 17.5 41.0 44-45 32.95608273542892 37.0 31.0 40.0 16.5 41.0 46-47 32.5248741836745 37.0 30.5 40.0 14.5 41.0 48-49 32.18192033125373 37.0 30.0 40.0 11.0 41.0 50-51 31.595301533052425 36.0 30.0 38.5 9.0 40.5 52-53 31.66717374786755 36.5 30.0 39.0 9.0 40.0 54-55 31.705200684983943 36.5 30.0 39.0 8.0 40.0 56-57 31.56321782836111 36.0 30.0 39.0 7.5 40.0 58-59 31.15886532019605 36.0 29.0 39.0 4.5 40.0 60-61 30.728810205134057 35.5 28.0 39.0 2.0 40.0 62-63 30.31154021716678 35.0 28.0 38.5 2.0 40.0 64-65 29.696277801291096 34.5 27.5 38.0 2.0 40.0 66-67 29.144746193370015 34.0 26.0 38.0 2.0 40.0 68-69 28.612615361725545 34.0 26.0 37.0 2.0 39.0 70-71 28.051228262015748 33.5 25.5 36.5 2.0 39.0 72-73 27.28446352554389 32.5 24.0 36.0 2.0 39.0 74-75 26.80820413446024 32.0 23.0 35.5 2.0 38.0 76-77 26.406598892931896 32.0 22.0 35.0 2.0 37.5 78-79 25.803302209080442 31.5 20.5 35.0 2.0 37.0 80-81 25.109947542825942 31.0 18.5 34.5 2.0 36.0 82-83 24.421997850577586 31.0 15.5 34.0 2.0 36.0 84-85 23.732581083638244 30.0 7.5 34.0 2.0 35.0 86-87 23.045596500832758 30.0 4.5 34.0 2.0 35.0 88-89 22.22178828911465 29.0 2.0 34.0 2.0 35.0 90-91 20.957467473374173 28.0 2.0 33.5 2.0 35.0 92-93 19.31749446393723 25.0 2.0 32.0 2.0 34.0 94-95 19.03474087037163 25.0 2.0 32.0 2.0 34.0 96-97 18.38180090685981 25.0 2.0 32.0 2.0 34.0 98-99 17.374770143251492 22.0 2.0 32.0 2.0 34.0 100-101 15.741202788182221 10.0 2.0 31.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.1228983011452378 1101 2 0.9792070629757212 1101 3 1.0134636186140256 1101 4 1.1426907183028874 1101 5 1.0449999339354576 1101 6 0.9121062960241915 1101 7 0.9602842804150455 1101 8 0.9478523048609304 1101 9 1.2127681816896896 1101 10-11 1.174103494563063 1101 12-13 0.9908118037724805 1101 14-15 1.195072463191984 1101 16-17 1.0645994400513814 1101 18-19 1.1296204734654083 1101 20-21 1.2734124402334572 1101 22-23 1.389055437288171 1101 24-25 1.3212197642592258 1101 26-27 1.232482785279558 1101 28-29 1.279689684541765 1101 30-31 1.428768769149194 1101 32-33 1.381726903883738 1101 34-35 1.4286177821303667 1101 36-37 1.5143026643160553 1101 38-39 1.2656097961211188 1101 40-41 1.4678225582184865 1101 42-43 1.5481282144490223 1101 44-45 1.3456179173464378 1101 46-47 1.2709069845122158 1101 48-49 1.406737689447425 1101 50-51 1.418883614250955 1101 52-53 1.4305679180610156 1101 54-55 1.7204932811154983 1101 56-57 1.616955123157112 1101 58-59 1.621741678211439 1101 60-61 1.2531839978045234 1101 62-63 1.2585763880978575 1101 64-65 1.132821518756252 1101 66-67 1.0379938149076153 1101 68-69 1.088405982185165 1101 70-71 1.051134122088083 1101 72-73 1.1943679164936398 1101 74-75 1.0261466297489328 1101 76-77 0.38281408244433024 1101 78-79 0.6860128628097648 1101 80-81 0.48626927491726946 1101 82-83 0.34263599007861956 1101 84-85 0.05367743638707978 1101 86-87 -0.35249283853079305 1101 88-89 -0.902218728723291 1101 90-91 -4.139538866139777 1101 92-93 -8.95942941967793 1101 94-95 -7.403786490765217 1101 96-97 -4.2297937949537 1101 98-99 -3.1079439617357636 1101 100-101 -2.7625189464546533 1102 1 1.1251986716456308 1102 2 1.0347719836375866 1102 3 1.0690577610580867 1102 4 1.143712486741535 1102 5 1.087115678432923 1102 6 1.1203882261885028 1102 7 1.2160522030087364 1102 8 1.0765800202701925 1102 9 1.3144289728565255 1102 10-11 1.1030366172345154 1102 12-13 1.055248119569697 1102 14-15 1.3343253874574756 1102 16-17 1.4506603318842721 1102 18-19 1.391442374058073 1102 20-21 1.3557726418359266 1102 22-23 1.3784963351403476 1102 24-25 1.463745925705794 1102 26-27 1.4337996937183703 1102 28-29 1.6053206265526256 1102 30-31 1.734241452348904 1102 32-33 1.603386744845686 1102 34-35 1.6067968039123244 1102 36-37 1.573720586293497 1102 38-39 1.4913257993605598 1102 40-41 1.5707140436272766 1102 42-43 1.631156541289286 1102 44-45 1.4647326603517996 1102 46-47 1.342461289119278 1102 48-49 1.3836460209154708 1102 50-51 1.4205587976424887 1102 52-53 1.3895165819496853 1102 54-55 1.5193036961562463 1102 56-57 1.5047213147080853 1102 58-59 1.5274791664183631 1102 60-61 1.438295042564743 1102 62-63 1.4001715276606923 1102 64-65 1.3817609867289775 1102 66-67 1.1939268107525542 1102 68-69 1.293648946395038 1102 70-71 1.3390426257062167 1102 72-73 1.1128628992925442 1102 74-75 0.9639733123769254 1102 76-77 0.7960541141390323 1102 78-79 0.7726494833881823 1102 80-81 0.5707641575272078 1102 82-83 0.4733067552161998 1102 84-85 -0.09912395846599154 1102 86-87 -0.2557270934568514 1102 88-89 -0.7766656518286545 1102 90-91 -4.773278912345976 1102 92-93 -10.173150676476965 1102 94-95 -8.831330584844249 1102 96-97 -5.30896444896899 1102 98-99 -3.691487347335668 1102 100-101 -3.02452661409127 1103 1 0.8855327227881169 1103 2 0.9664720103876832 1103 3 0.8656690577100896 1103 4 0.9523666944839135 1103 5 0.8168623219221374 1103 6 0.8518946502374689 1103 7 0.8532286036386907 1103 8 0.8576473341877815 1103 9 0.9415740875046907 1103 10-11 0.8469741568042295 1103 12-13 0.8923043236982764 1103 14-15 1.029078611112432 1103 16-17 0.9873966829982166 1103 18-19 1.024671048013765 1103 20-21 1.0504053111286922 1103 22-23 1.012558079252571 1103 24-25 0.9784656356954002 1103 26-27 0.9632870521359536 1103 28-29 1.2171790454817994 1103 30-31 1.171956597525579 1103 32-33 1.1921133388844893 1103 34-35 1.1569415287904192 1103 36-37 1.237968784837058 1103 38-39 1.28120946179164 1103 40-41 1.134432802005314 1103 42-43 1.2218726923530738 1103 44-45 1.07865675628355 1103 46-47 1.1248051969414874 1103 48-49 0.9962565439916702 1103 50-51 0.9815475253250234 1103 52-53 1.0807586691251743 1103 54-55 0.9828591507155231 1103 56-57 0.8442594327001629 1103 58-59 1.0795857886625733 1103 60-61 0.8453357751774035 1103 62-63 1.0295038428800076 1103 64-65 0.789420743997951 1103 66-67 0.9554389695210865 1103 68-69 0.7943440164746249 1103 70-71 0.8732271309889903 1103 72-73 0.9972461962582742 1103 74-75 0.8333401730936174 1103 76-77 0.7559108535081087 1103 78-79 0.6736137393552859 1103 80-81 0.44673944008068744 1103 82-83 0.32798238569985827 1103 84-85 0.15868337165673552 1103 86-87 -0.07174510757386798 1103 88-89 -0.6111038804295497 1103 90-91 -2.2992580419032222 1103 92-93 -9.039951824255887 1103 94-95 -5.425456180272459 1103 96-97 -2.7784190154071933 1103 98-99 -1.7025982546649256 1103 100-101 -1.2428452706579112 1104 1 0.8251496103715326 1104 2 0.6526280665289335 1104 3 0.7596466780556987 1104 4 0.7092395724009037 1104 5 0.6940975217235348 1104 6 0.7542366692501687 1104 7 0.7586931128429981 1104 8 0.7031772877793117 1104 9 0.8282559743243212 1104 10-11 0.5358706225525154 1104 12-13 0.6485437854200242 1104 14-15 0.717597915768657 1104 16-17 0.811059807756422 1104 18-19 0.7806835193056898 1104 20-21 0.7910142837532277 1104 22-23 0.7873973222208832 1104 24-25 0.7909079026126804 1104 26-27 0.7669706672111474 1104 28-29 0.7899378981400957 1104 30-31 0.8502587469527683 1104 32-33 0.8156176397192851 1104 34-35 0.6814310147627438 1104 36-37 0.7044360733023538 1104 38-39 0.45302570184370694 1104 40-41 0.43722598893707243 1104 42-43 0.42355012577057494 1104 44-45 0.22547919931110272 1104 46-47 0.271847881864268 1104 48-49 0.38084316849703015 1104 50-51 0.4350904239934401 1104 52-53 0.5113323942166232 1104 54-55 0.6377139897364863 1104 56-57 0.7451483093113467 1104 58-59 0.5070137505786327 1104 60-61 0.498805966269412 1104 62-63 0.4073111544315928 1104 64-65 0.3613808728987493 1104 66-67 0.3923483702549966 1104 68-69 0.4476029216705797 1104 70-71 0.32461396746139926 1104 72-73 0.34307283309011183 1104 74-75 0.2259660682678124 1104 76-77 0.16835599643639299 1104 78-79 -0.10140538044154468 1104 80-81 -0.09084226606742973 1104 82-83 -0.26885252634907886 1104 84-85 -0.3357613229670271 1104 86-87 -0.3744003595042287 1104 88-89 -0.3740179582132832 1104 90-91 -0.6708927779100051 1104 92-93 -4.026457971666336 1104 94-95 -1.774919205051436 1104 96-97 -1.0325359683946296 1104 98-99 -0.8510581318516905 1104 100-101 -0.656990035176312 1105 1 0.9667150849851645 1105 2 0.9120953061122989 1105 3 0.9428810475981351 1105 4 0.7721718578409735 1105 5 0.5979972803267515 1105 6 0.6053455372785308 1105 7 0.6437273184763299 1105 8 0.7494602053116921 1105 9 1.0533696777332224 1105 10-11 0.8417751346437612 1105 12-13 0.8002640554915956 1105 14-15 0.9093764660106487 1105 16-17 1.0738703091060984 1105 18-19 0.9080856220820337 1105 20-21 0.7575770751463295 1105 22-23 1.04035286637491 1105 24-25 0.8881138790288787 1105 26-27 1.0131964461050913 1105 28-29 1.0109857624516465 1105 30-31 1.008508641950435 1105 32-33 1.1861584218796182 1105 34-35 1.0668777229009336 1105 36-37 1.0896441144024678 1105 38-39 1.0660431989231824 1105 40-41 0.916989232724255 1105 42-43 1.1433364111324806 1105 44-45 0.9743984973433797 1105 46-47 0.932062018372882 1105 48-49 0.7791192070584785 1105 50-51 0.6836286736899133 1105 52-53 0.7183789345992437 1105 54-55 1.0362020802698986 1105 56-57 0.905917205542984 1105 58-59 0.7410891624142124 1105 60-61 0.6702166082012333 1105 62-63 0.7315274524061799 1105 64-65 0.7403220895857103 1105 66-67 0.8267782774000558 1105 68-69 0.7275849797658722 1105 70-71 0.665006517972639 1105 72-73 0.7900041283752728 1105 74-75 0.6755987617435188 1105 76-77 0.39795461766429696 1105 78-79 0.2693679888583169 1105 80-81 0.2323656993441503 1105 82-83 0.36817374563704774 1105 84-85 0.3784317659254306 1105 86-87 0.35532457471808954 1105 88-89 0.04847717011289987 1105 90-91 -1.3228608969072724 1105 92-93 -7.06585366697408 1105 94-95 -2.934528329614288 1105 96-97 -1.2425386423091993 1105 98-99 -0.6804519597620207 1105 100-101 -0.7244258503697303 1106 1 0.8815339836316305 1106 2 0.833654945142392 1106 3 0.7956146652794089 1106 4 0.9456671680283932 1106 5 0.808803649849132 1106 6 0.9033537013196948 1106 7 0.8305349607461494 1106 8 0.7520442682823827 1106 9 0.939906172457313 1106 10-11 0.8057945371549593 1106 12-13 0.9642207848337279 1106 14-15 1.0360133204511826 1106 16-17 1.2153547816562877 1106 18-19 1.0458935901145807 1106 20-21 1.0671895552338242 1106 22-23 1.108676882526673 1106 24-25 1.03439932294431 1106 26-27 0.9600498742241257 1106 28-29 1.0049673475594005 1106 30-31 0.9881065970144576 1106 32-33 0.8207272890173201 1106 34-35 0.874312826900514 1106 36-37 0.9035153396353692 1106 38-39 0.9267636511066542 1106 40-41 0.9987608448090199 1106 42-43 1.0121531348133601 1106 44-45 0.8262209435667032 1106 46-47 0.6462888277507162 1106 48-49 0.625881311416876 1106 50-51 0.7055633875318001 1106 52-53 0.6628076686632127 1106 54-55 0.684787216260343 1106 56-57 0.7728746711226719 1106 58-59 0.7717475681603077 1106 60-61 0.8608160460711183 1106 62-63 0.7599830684386646 1106 64-65 0.7326192816211226 1106 66-67 0.6910052589569275 1106 68-69 0.6957809635865502 1106 70-71 0.8054707952993461 1106 72-73 0.8671268376192884 1106 74-75 0.773879932864002 1106 76-77 0.5369170805683225 1106 78-79 0.609145202960196 1106 80-81 0.8148530941169341 1106 82-83 0.9032023996721996 1106 84-85 0.5477756329426064 1106 86-87 0.37490408596379154 1106 88-89 0.5083650333504046 1106 90-91 0.07766583319933318 1106 92-93 -3.4605513102563084 1106 94-95 -1.219189519151037 1106 96-97 -0.10989749165736384 1106 98-99 -0.05746233940302048 1106 100-101 0.08976066911370673 1107 1 0.6077629493616818 1107 2 0.5975611631050342 1107 3 0.5655686566731788 1107 4 0.6655782230187981 1107 5 0.496184813456118 1107 6 0.5457452285360986 1107 7 0.5447406508177224 1107 8 0.48508120847660763 1107 9 0.457012590451491 1107 10-11 0.4307059744789754 1107 12-13 0.4858170175280634 1107 14-15 0.5173454925730354 1107 16-17 0.7149528915071599 1107 18-19 0.4115134070410633 1107 20-21 0.3943319937914538 1107 22-23 0.46395244355895926 1107 24-25 0.5928547492733571 1107 26-27 0.4732006618652136 1107 28-29 0.5494601532588064 1107 30-31 0.5447908374935153 1107 32-33 0.35693421473700226 1107 34-35 0.3907237360627889 1107 36-37 0.5301361804039075 1107 38-39 0.4690649069879882 1107 40-41 0.4101748904603326 1107 42-43 0.3089457279623531 1107 44-45 0.13264604305803118 1107 46-47 0.29325563645600994 1107 48-49 0.41078576523131716 1107 50-51 0.33940782985592577 1107 52-53 0.47488189958053795 1107 54-55 0.6901464508352113 1107 56-57 0.38330182235829113 1107 58-59 0.4686568238142783 1107 60-61 0.6000273019727587 1107 62-63 0.4495498269330689 1107 64-65 0.4637555162237348 1107 66-67 0.35809269560965973 1107 68-69 0.3651691012293625 1107 70-71 0.4744219222361359 1107 72-73 0.4951436359372927 1107 74-75 0.4582127810058232 1107 76-77 0.2810258609105567 1107 78-79 0.14137782149883904 1107 80-81 0.024481607948949602 1107 82-83 0.18670697109651968 1107 84-85 -0.17041742455563025 1107 86-87 -0.39701780700038825 1107 88-89 -0.40338566889155913 1107 90-91 -0.7137768637887589 1107 92-93 -4.1756959169500565 1107 94-95 -1.8209008850158845 1107 96-97 -1.1140490933645353 1107 98-99 -0.9355004875007928 1107 100-101 -0.7903476294205021 1108 1 0.28886326364041537 1108 2 0.3252984453337575 1108 3 0.09119565433971033 1108 4 -0.3099369142352657 1108 5 -0.9711175412278479 1108 6 -0.16239090068362572 1108 7 0.02051444856339657 1108 8 0.2642004793071564 1108 9 0.21005546106598416 1108 10-11 0.1918193733063518 1108 12-13 0.1424658043550906 1108 14-15 0.1594575954240085 1108 16-17 0.41122539951565074 1108 18-19 0.352737400609314 1108 20-21 0.10075001838113451 1108 22-23 0.11808361348517593 1108 24-25 0.13628600280520686 1108 26-27 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0.5555980758826138 2311 74-75 0.6457367452764942 2311 76-77 0.7403506487784028 2311 78-79 0.5771570149238165 2311 80-81 0.7103609569804945 2311 82-83 0.7683213247274807 2311 84-85 0.7920332498827669 2311 86-87 1.0685527360751195 2311 88-89 1.2896593194594423 2311 90-91 1.8553717705702049 2311 92-93 2.8681000235239864 2311 94-95 2.2156838517854958 2311 96-97 1.8700274547488434 2311 98-99 1.5813265141068555 2311 100-101 1.5353498060801467 2312 1 0.24480210018256088 2312 2 0.16419752798412546 2312 3 0.1723849640472217 2312 4 0.05730697417504871 2312 5 0.1286598999187305 2312 6 0.19958204999249318 2312 7 0.1931359091575473 2312 8 -0.3901482193436223 2312 9 0.021782205732705506 2312 10-11 -0.00788591512227299 2312 12-13 0.03641092117292999 2312 14-15 -0.1544421761942516 2312 16-17 0.013391836822691516 2312 18-19 0.19253947569170293 2312 20-21 0.27747856103180624 2312 22-23 0.15348984006582356 2312 24-25 0.2764041252387983 2312 26-27 0.37567430158834725 2312 28-29 0.27048349930522875 2312 30-31 0.3410750523126822 2312 32-33 0.34103694007522023 2312 34-35 0.1654148514875331 2312 36-37 0.32711756417035787 2312 38-39 0.4752053477992604 2312 40-41 0.6031396925418164 2312 42-43 0.5319604482517946 2312 44-45 0.3849332584376626 2312 46-47 0.4337239585571737 2312 48-49 0.3720137736520215 2312 50-51 0.4226463846657076 2312 52-53 0.2904505251552436 2312 54-55 0.26336986614285607 2312 56-57 0.26591736785881537 2312 58-59 0.5081704687320077 2312 60-61 0.5362283691424814 2312 62-63 0.5054301394287961 2312 64-65 0.5502637022630417 2312 66-67 0.46616624237693927 2312 68-69 0.23174913440184497 2312 70-71 0.4702010882762657 2312 72-73 0.3431140996685613 2312 74-75 0.4771496894944285 2312 76-77 0.6552410332206442 2312 78-79 0.6209099892386014 2312 80-81 0.6791451214070463 2312 82-83 0.7321706454047039 2312 84-85 0.889477590002901 2312 86-87 0.713377642719152 2312 88-89 1.0750132575593945 2312 90-91 1.643900937076097 2312 92-93 2.68104702820143 2312 94-95 2.0159195461352617 2312 96-97 1.6605848359799005 2312 98-99 1.5886616566810368 2312 100-101 1.4733998131541934 2313 1 -0.24561236498057326 2313 2 -0.980711088203865 2313 3 -0.3135493985960913 2313 4 -0.1968638899108086 2313 5 -0.9654633705732394 2313 6 -0.45634008017204053 2313 7 -1.2629856412491733 2313 8 -1.182382822524751 2313 9 -1.2954767253473847 2313 10-11 -2.044410314976993 2313 12-13 -0.5710682801866938 2313 14-15 -0.2173250836180287 2313 16-17 -1.5195780342737137 2313 18-19 -1.0866702787678406 2313 20-21 -1.448656112734497 2313 22-23 -1.200606584075672 2313 24-25 -0.2821108963821928 2313 26-27 -0.9845790512073052 2313 28-29 -2.4651009356364852 2313 30-31 -0.8514519996256737 2313 32-33 -0.5797534767884613 2313 34-35 -0.1442129145260438 2313 36-37 -0.19161063494835417 2313 38-39 -0.6936803830313849 2313 40-41 -0.2613072246679451 2313 42-43 -0.04810275288853916 2313 44-45 -0.7147100465930691 2313 46-47 -0.8730594134952057 2313 48-49 -0.3093922589816316 2313 50-51 -0.3187099299659799 2313 52-53 -0.20564159192019815 2313 54-55 -0.212103071639401 2313 56-57 -0.5171030435910602 2313 58-59 -0.19354893122550365 2313 60-61 -0.3503839195303122 2313 62-63 -0.29166319061513235 2313 64-65 -0.2021656611683582 2313 66-67 -0.27503416811352466 2313 68-69 -0.11637110709538234 2313 70-71 -0.13553018780769932 2313 72-73 0.21730631062913375 2313 74-75 0.1391983384394173 2313 76-77 0.14454446722350767 2313 78-79 0.09662063800541532 2313 80-81 0.1516177610868752 2313 82-83 0.20907113401856137 2313 84-85 0.3963416552976078 2313 86-87 0.3471690943751611 2313 88-89 0.3610343954197752 2313 90-91 0.9947670616760576 2313 92-93 2.2956644120271434 2313 94-95 1.6274856631970032 2313 96-97 0.9848534571019982 2313 98-99 0.7759826239034773 2313 100-101 0.6729215643517445 2314 1 -0.5075306097890113 2314 2 -0.6755904097955145 2314 3 -1.7085214312016035 2314 4 -0.602766705141093 2314 5 -0.2992230204800137 2314 6 -2.8334312143282183 2314 7 -3.755605998636007 2314 8 -1.4210218710683407 2314 9 -1.8239449133992238 2314 10-11 -1.0694349052251297 2314 12-13 -2.174935433778799 2314 14-15 -2.707925252603758 2314 16-17 -1.9989209417527434 2314 18-19 -2.4622211516895476 2314 20-21 -2.212236927975056 2314 22-23 -3.2417814457796013 2314 24-25 -1.8357593701174935 2314 26-27 -1.9386989771412146 2314 28-29 -1.2727095307949696 2314 30-31 -1.42087771781501 2314 32-33 -2.237788516928994 2314 34-35 -0.7559303282739833 2314 36-37 -0.4219413256325737 2314 38-39 -0.467420887214935 2314 40-41 -0.8186911047651222 2314 42-43 -0.6898459510846493 2314 44-45 -1.1371756459273925 2314 46-47 -1.4876689100821068 2314 48-49 -1.8548932066371435 2314 50-51 -1.1592537275734713 2314 52-53 -0.7640142182871088 2314 54-55 -0.5869342143219072 2314 56-57 -0.6730036116707332 2314 58-59 -0.8650829261199782 2314 60-61 -0.8679059159245988 2314 62-63 -0.8883019027281698 2314 64-65 -1.0730482392022687 2314 66-67 -0.8325259114401291 2314 68-69 -0.5822564715960539 2314 70-71 -0.6509963624125881 2314 72-73 -0.4365066545310441 2314 74-75 -0.4012311113040248 2314 76-77 -0.3668315722441555 2314 78-79 -0.3689369635272719 2314 80-81 -0.19761191568297676 2314 82-83 -0.20661738008841013 2314 84-85 -0.24090082124213907 2314 86-87 -0.1514216211689572 2314 88-89 0.012215947212101952 2314 90-91 0.5772041029363137 2314 92-93 1.4876413780913182 2314 94-95 0.7327104167808862 2314 96-97 0.4963900073277401 2314 98-99 0.5994878835199948 2314 100-101 0.7395999804059006 2315 1 -7.17436363601329 2315 2 -3.210269816302503 2315 3 -1.4832708018502743 2315 4 -0.9702962403121731 2315 5 -1.5017753062730321 2315 6 -1.7929448856550678 2315 7 -2.9971899194133016 2315 8 -2.8547580654474167 2315 9 -3.3630309433024586 2315 10-11 -2.3791976714163 2315 12-13 -2.786882822674528 2315 14-15 -4.852142374261696 2315 16-17 -4.604797265062597 2315 18-19 -3.490771605410899 2315 20-21 -4.282777507642869 2315 22-23 -5.4552434051057475 2315 24-25 -3.6379500249434216 2315 26-27 -4.796556799405714 2315 28-29 -3.0518294379384017 2315 30-31 -2.793196439868826 2315 32-33 -1.9646443255232242 2315 34-35 -2.6942862810041674 2315 36-37 -3.2650404518004237 2315 38-39 -2.357639664848932 2315 40-41 -3.626256485552844 2315 42-43 -7.745302946715704 2315 44-45 -4.2851099300923785 2315 46-47 -2.9054761768199704 2315 48-49 -5.704161182252982 2315 50-51 -6.815657215959838 2315 52-53 -6.966493060356857 2315 54-55 -5.0336096265062835 2315 56-57 -4.233948144515743 2315 58-59 -4.377311094391192 2315 60-61 -3.4370621766527805 2315 62-63 -3.620021506022006 2315 64-65 -2.4334058995519285 2315 66-67 -2.9824422236932087 2315 68-69 -2.40977282922762 2315 70-71 -2.1173467554317504 2315 72-73 -2.6084936522405293 2315 74-75 -3.0908385704771426 2315 76-77 -2.916488841924007 2315 78-79 -2.018881319853353 2315 80-81 -1.8877704144340122 2315 82-83 -2.5231560176247996 2315 84-85 -2.3187189965952726 2315 86-87 -2.6304805298079295 2315 88-89 -2.087650266685653 2315 90-91 -1.495110237892515 2315 92-93 -0.6157847398512573 2315 94-95 -0.818075494554126 2315 96-97 -0.8398344053658704 2315 98-99 -0.6956540787523657 2315 100-101 -0.40215910470966065 2316 1 -4.416880032524549 2316 2 -2.873296639534665 2316 3 -4.705442491980158 2316 4 -4.401492017538992 2316 5 -4.304277396282867 2316 6 -4.592088828394971 2316 7 -3.008194911884331 2316 8 -1.8435489507390201 2316 9 -2.0684548524765844 2316 10-11 -3.0949453992105447 2316 12-13 -4.154837773569671 2316 14-15 -3.4012572127595107 2316 16-17 -3.0386445565063127 2316 18-19 -3.315785742558166 2316 20-21 -5.5333622842064045 2316 22-23 -6.499862843868293 2316 24-25 -4.406753440200056 2316 26-27 -4.029007894745831 2316 28-29 -4.5477845651413205 2316 30-31 -6.137291688239607 2316 32-33 -4.501153942933101 2316 34-35 -6.202995290847568 2316 36-37 -9.470481969321398 2316 38-39 -6.40498161199114 2316 40-41 -5.768622941943697 2316 42-43 -4.892082088473664 2316 44-45 -3.795953531409797 2316 46-47 -5.966486631243178 2316 48-49 -3.552346221528566 2316 50-51 -4.542427544571968 2316 52-53 -5.304588797275144 2316 54-55 -6.973663467671816 2316 56-57 -5.122757939153068 2316 58-59 -6.250466003205748 2316 60-61 -6.5891695256589635 2316 62-63 -6.681798091765792 2316 64-65 -5.402687465541842 2316 66-67 -6.943336945294032 2316 68-69 -5.714026332896097 2316 70-71 -6.508778062803277 2316 72-73 -8.385671462672882 2316 74-75 -8.35619691828035 2316 76-77 -7.408730963198188 2316 78-79 -6.243262240659984 2316 80-81 -6.571470165875937 2316 82-83 -5.6037403926731635 2316 84-85 -6.951504829973654 2316 86-87 -5.493648477771565 2316 88-89 -5.280024652440343 2316 90-91 -5.82123622438052 2316 92-93 -4.615811825301986 2316 94-95 -4.565642570579342 2316 96-97 -5.3277404431895 2316 98-99 -6.241279436386861 2316 100-101 -5.2636414740439506 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 16780.0 3 6780.0 4 7096.0 5 7819.0 6 8775.0 7 10261.0 8 12289.0 9 14989.0 10 17882.0 11 20934.0 12 24226.0 13 26555.0 14 29269.0 15 32114.0 16 35776.0 17 39278.0 18 37913.0 19 37361.0 20 39415.0 21 42658.0 22 46935.0 23 52498.0 24 58052.0 25 65118.0 26 72979.0 27 82319.0 28 93189.0 29 105833.0 30 120924.0 31 137347.0 32 158632.0 33 183588.0 34 214835.0 35 257909.0 36 300582.0 37 265282.0 38 83490.0 39 1434.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.5606754735135 19.480495071584997 13.598926667801589 23.35990278709992 2 14.968947310013963 20.766570019740264 41.03339795338897 23.2310847168568 3 19.200775851723545 25.479991382638328 29.734090774362482 25.585141991275645 4 12.115444953883035 16.89804673436368 38.991680294269464 31.99482801748382 5 13.036393578675396 38.160520102424265 35.09961207750354 13.70347424139681 6 30.609341095413157 37.54616987042801 18.230864531275525 13.613624502883312 7 27.476516539267298 32.97291865765835 21.985897634231996 17.56466716884236 8 25.302894872952404 35.734022862459106 20.49373282939106 18.46934943519743 9 26.23810344746579 16.703730167593527 20.73853769218898 36.31962869275171 10-11 24.268768531876862 26.94133467645672 28.548908070686025 20.240988720980393 12-13 25.54321641368916 24.394796717406745 28.470478527784753 21.591508341119344 14-15 22.523104116043342 25.686065961155624 26.816534296746582 24.97429562605445 16-17 21.80935389357609 28.808310025829222 27.317220131032872 22.065115949561815 18-19 21.62685743626038 27.943898326741785 29.236917408098932 21.192326828898896 20-21 22.596969646579264 27.11959897016858 29.050496732432535 21.232934650819622 22-23 22.08511017519174 27.076548254328003 28.925066336132353 21.9132752343479 24-25 21.952415486582378 27.18937344652596 29.076531948927713 21.78167911796395 26-27 21.98446165382292 27.253927214201674 29.147011082467657 21.61460004950775 28-29 21.704339775823755 27.48413787616604 29.170811883980928 21.64071046402928 30-31 22.003483655874977 27.262165808349742 29.02561967974451 21.70873085603077 32-33 21.90729636890226 27.302375468618855 28.999384155176983 21.790944007301903 34-35 22.201879781357533 27.304616138984635 28.9131555766413 21.58034850301653 36-37 22.16538314663951 27.11462576374745 29.036542711997775 21.683448377615257 38-39 21.85511175699861 27.507370644666313 29.04472431429546 21.59279328403961 40-41 22.04902607903638 27.367684383836643 29.03427240716063 21.549017129966348 42-43 21.891580103089858 27.384149559298184 28.794615940473932 21.92965439713803 44-45 22.0074329819548 27.42249356496972 28.68833122342313 21.88174222965235 46-47 22.14206676092703 27.49161962688807 28.425647015430012 21.940666596754888 48-49 22.03715434876449 27.603615574901042 28.408336517628396 21.950893558706074 50-51 21.916867012504003 27.742350641296582 28.392355551600783 21.94842679459863 52-53 22.085233324766 27.938705165522858 28.069240930097617 21.906820579613523 54-55 22.007130107367605 27.624725784231323 28.188666598299246 22.17947751010183 56-57 21.983430667928257 27.888978712717062 28.05425978003388 22.0733308393208 58-59 22.190462346648616 27.689723974740595 28.12066992967749 21.999143748933303 60-61 22.101807777494738 27.66942908378858 28.35245523313194 21.876307905584742 62-63 21.982998705043748 27.619032295445994 28.35865605849883 22.039312941011424 64-65 21.82011003209571 27.705106319289747 28.36365036430327 22.11113328431128 66-67 21.877078445023475 27.822269595527704 27.924574964489608 22.376076994959217 68-69 21.84693230988113 27.75639258406918 28.13803588651033 22.258639219539354 70-71 21.949639108056417 27.584716904618688 28.10267363591379 22.362970351411107 72-73 22.01005713971561 27.48911133600164 28.129464605422015 22.371366918860737 74-75 21.84514459194387 27.94870890522144 27.82562691778648 22.380519585048205 76-77 21.891585915346727 27.608350687036626 27.94713452721024 22.552928870406408 78-79 21.959487140251806 27.67194035989606 27.94505751105627 22.423514988795866 80-81 21.950119646066874 27.745496925053576 27.78693756897353 22.517445859906022 82-83 21.99892555196602 27.74581164238607 27.87841734166362 22.376845463984285 84-85 22.021316072917926 27.73635718527724 27.66915672463942 22.573170017165413 86-87 21.79210235397283 27.64391312108329 27.905592247498177 22.658392277445703 88-89 21.95636347193018 27.54149539894639 27.7793122159669 22.722828913156526 90-91 21.737031193567397 27.51241687010691 28.101945513316622 22.648606423009067 92-93 21.529561628837328 27.564352956380695 28.36586980860014 22.540215606181835 94-95 21.832881708064193 27.498489206303784 27.79317763370547 22.875451451926555 96-97 21.823123113290233 27.410488527918194 27.777378785200867 22.989009573590703 98-99 21.833730789133714 27.344838442359258 27.57811906304168 23.24331170546535 100-101 21.886512289758073 27.46671405107564 27.64502080999473 23.001752849171552 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2556.0 1 1527.5 2 500.5 3 522.5 4 482.5 5 452.0 6 653.5 7 1143.0 8 1814.5 9 2150.0 10 2013.0 11 1856.5 12 1892.0 13 2086.5 14 2271.0 15 2430.0 16 2602.0 17 2862.0 18 3264.5 19 3683.0 20 4219.0 21 5072.5 22 6086.0 23 7612.0 24 9502.0 25 11769.0 26 15433.0 27 19996.0 28 24553.0 29 29383.5 30 34710.0 31 40528.0 32 46917.0 33 53925.0 34 61484.0 35 68940.5 36 77120.0 37 83802.5 38 86815.5 39 88909.5 40 90990.5 41 93183.5 42 95133.5 43 97738.0 44 101134.0 45 103406.0 46 104780.5 47 107564.5 48 113337.5 49 112068.5 50 103336.5 51 96357.0 52 91629.0 53 87680.0 54 82107.5 55 75383.5 56 68095.0 57 60927.5 58 54694.0 59 48605.5 60 41398.0 61 33015.0 62 25456.0 63 19342.0 64 14357.0 65 10466.0 66 7569.0 67 5526.0 68 3884.0 69 2638.5 70 1707.5 71 1134.5 72 752.0 73 475.0 74 305.5 75 205.0 76 147.0 77 86.5 78 59.0 79 47.5 80 31.5 81 20.0 82 13.0 83 12.0 84 8.5 85 7.5 86 6.5 87 4.5 88 2.5 89 3.5 90 6.5 91 4.5 92 1.5 93 2.0 94 1.5 95 0.0 96 0.5 97 1.5 98 1.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08685082170627738 2 0.09710680231525151 3 0.0941816810852272 4 0.09649288798302419 5 0.09331497849855333 6 0.07800323280064829 7 0.08984816815185785 8 0.08656192084405276 9 0.08100057924622875 10-11 0.08777169320461836 12-13 0.08670637127516508 14-15 0.09257467003910273 16-17 0.08963149250518938 18-19 0.09271912047021505 20-21 0.10248758087418512 22-23 0.11156990173037172 24-25 0.1351153220016785 26-27 0.1405683257761683 28-29 0.14015303078672037 30-31 0.14147114097062022 32-33 0.13755292302669878 34-35 0.13318329748555133 36-37 0.1373723599878084 38-39 0.12944564258052027 40-41 0.12514824225492902 42-43 0.1253288052938194 44-45 0.11557840119373836 46-47 0.1345555765811183 48-49 0.1532799637140517 50-51 0.1632831560685793 52-53 0.1711557045642003 54-55 0.16761666900194863 56-57 0.13818489366281514 58-59 0.12899423498329432 60-61 0.12576215658715634 62-63 0.12225923363268278 64-65 0.10884339984312684 66-67 0.12193422016268007 68-69 0.11965912587266117 70-71 0.12774835001495063 72-73 0.1557897899546281 74-75 0.1696750876452991 76-77 0.17393637536311227 78-79 0.17097514152530988 80-81 0.17772819917981045 82-83 0.17716845375925022 84-85 0.15362303348794346 86-87 0.15454390498628442 88-89 0.17346691146199728 90-91 0.26176223747939775 92-93 0.5223327589021189 94-95 0.17480307794978614 96-97 0.18816474282767498 98-99 0.20605853998171259 100-101 0.21308244219454872 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2769116.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.719700923301154 #Duplication Level Percentage of deduplicated Percentage of total 1 78.68370928822536 47.776512955170176 2 11.939977879715027 14.499837717742558 3 4.105706418847127 7.478917974938263 4 1.8711852655666494 4.544712387891792 5 0.9765990777660836 2.9649401961964164 6 0.5983015634424101 2.1797215196499984 7 0.3760929100677726 1.5985374313082408 8 0.2718351842131875 1.3204600868684178 9 0.19279628127733095 1.0535879284455762 >10 0.8947486866574181 9.571655224057114 >50 0.05779000932002859 2.3971835710108307 >100 0.02951106417209733 3.0806806297892684 >500 0.0011441739262825035 0.4535548622705221 >1k 4.8175744258772746E-4 0.5807286618748784 >5k 1.2043936064693186E-4 0.49896885278583236 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8013 0.2893703261257383 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5633 0.20342231961391288 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3963 0.14311426462452279 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3764 0.13592785567668528 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 7.222521555615583E-5 0.0 3 0.0 0.0 0.0 7.222521555615583E-5 0.0 4 0.0 0.0 0.0 1.0833782333423375E-4 0.0 5 0.0 0.0 0.0 1.0833782333423375E-4 0.0 6 0.0 0.0 0.0 1.0833782333423375E-4 0.0 7 0.0 3.6112607778077916E-5 0.0 1.4445043111231166E-4 0.0 8 0.0 3.6112607778077916E-5 0.0 1.4445043111231166E-4 0.0 9 0.0 3.6112607778077916E-5 0.0 2.166756466684675E-4 0.0 10-11 0.0 3.6112607778077916E-5 0.0 2.166756466684675E-4 0.0 12-13 0.0 5.4168911667116874E-5 0.0 2.527882544465454E-4 0.0 14-15 0.0 7.222521555615583E-5 0.0 2.889008622246233E-4 0.0 16-17 0.0 7.222521555615583E-5 0.0 3.6112607778077913E-4 0.0 18-19 0.0 7.222521555615583E-5 0.0 4.33351293336935E-4 0.0 20-21 0.0 7.222521555615583E-5 0.0 5.778017244492467E-4 0.0 22-23 0.0 7.222521555615583E-5 0.0 9.389278022300258E-4 0.0 24-25 0.0 7.222521555615583E-5 0.0 0.0013542227916779218 0.0 26-27 0.0 7.222521555615583E-5 0.0 0.001751461477236779 0.0 28-29 0.0 7.222521555615583E-5 0.0 0.0031056842689147005 0.0 30-31 0.0 7.222521555615583E-5 0.0 0.007150296340059427 0.0 32-33 0.0 7.222521555615583E-5 0.0 0.015618702864018699 0.0 34-35 0.0 7.222521555615583E-5 0.0 0.029160930780797914 0.0 36-37 0.0 7.222521555615583E-5 0.0 0.05146046608376103 0.0 38-39 0.0 7.222521555615583E-5 0.0 0.08455767111236943 0.0 40-41 0.0 7.222521555615583E-5 0.0 0.12045360324377888 0.0 42-43 0.0 7.222521555615583E-5 0.0 0.15793849011742375 0.0 44-45 0.0 7.222521555615583E-5 0.0 0.2060224273739345 0.0 46-47 0.0 7.222521555615583E-5 0.0 0.26306229135940856 0.0 48-49 0.0 7.222521555615583E-5 0.0 0.3281010979677269 0.0 50-51 0.0 7.222521555615583E-5 0.0 0.3965525460110736 0.0 52-53 0.0 7.222521555615583E-5 0.0 0.46404700994830117 0.0 54-55 0.0 7.222521555615583E-5 0.0 0.5425558192578426 1.8056303889038958E-5 56-57 0.0 7.222521555615583E-5 0.0 0.6357082910213946 3.6112607778077916E-5 58-59 0.0 7.222521555615583E-5 0.0 0.7403445720583752 3.6112607778077916E-5 60-61 0.0 7.222521555615583E-5 0.0 0.8579091666799079 3.6112607778077916E-5 62-63 0.0 7.222521555615583E-5 0.0 0.9770807723475651 3.6112607778077916E-5 64-65 1.8056303889038958E-5 7.222521555615583E-5 0.0 1.1019942826519364 3.6112607778077916E-5 66-67 3.6112607778077916E-5 7.222521555615583E-5 0.0 1.239926388060305 3.6112607778077916E-5 68-69 3.6112607778077916E-5 7.222521555615583E-5 0.0 1.3977204277466166 3.6112607778077916E-5 70-71 3.6112607778077916E-5 7.222521555615583E-5 0.0 1.5806127298386923 3.6112607778077916E-5 72-73 3.6112607778077916E-5 7.222521555615583E-5 0.0 1.768181614638029 3.6112607778077916E-5 74-75 3.6112607778077916E-5 7.222521555615583E-5 0.0 1.9640202866185454 3.6112607778077916E-5 76-77 3.6112607778077916E-5 7.222521555615583E-5 0.0 2.170638572020818 3.6112607778077916E-5 78-79 3.6112607778077916E-5 7.222521555615583E-5 0.0 2.4100109926778077 3.6112607778077916E-5 80-81 3.6112607778077916E-5 7.222521555615583E-5 0.0 2.674951139641676 3.6112607778077916E-5 82-83 3.6112607778077916E-5 7.222521555615583E-5 0.0 2.9436289415105756 3.6112607778077916E-5 84-85 3.6112607778077916E-5 7.222521555615583E-5 0.0 3.2113497592733564 3.6112607778077916E-5 86-87 3.6112607778077916E-5 1.263941272232727E-4 0.0 3.4973255002679555 3.6112607778077916E-5 88-89 3.6112607778077916E-5 1.4445043111231166E-4 0.0 3.814412252863369 3.6112607778077916E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACATGG 9290 0.0 27.126135 2 GTACATG 9575 0.0 26.963064 1 GTATCAA 14785 0.0 26.128363 1 ACATGGG 9555 0.0 25.181778 3 ATCAACG 16795 0.0 22.747044 3 TATCAAC 16785 0.0 22.732323 2 CAACGCA 16860 0.0 22.63079 5 TCAACGC 16875 0.0 22.610674 4 AACGCAG 17020 0.0 22.443495 6 GGTATCA 10595 0.0 21.366163 1 GAGTACT 7120 0.0 20.59585 12-13 CATGGGA 5990 0.0 20.202942 4 ACGCAGA 18785 0.0 19.80428 7 AGTACTT 7635 0.0 19.517397 12-13 CGCAGAG 18740 0.0 19.473423 8 AGAGTAC 12425 0.0 19.23076 10-11 GTACTTT 7925 0.0 18.623547 14-15 GCAGAGT 18845 0.0 17.98024 9 CATGGGG 4650 0.0 17.8602 4 TACACCG 350 1.3296813E-9 17.626925 5 >>END_MODULE