FastQCFastQC Report
Fri 27 May 2016
SRR939591_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939591_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2769116
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85780.30977394952035237No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64190.23180682932748214No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46880.16929590526362925No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT41540.15001177271013566No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA95900.026.1742921
GTACATG96350.025.3677521
TACATGG93300.025.082932
GTATCAA135800.024.7954881
ACATGGG94250.024.028143
ATCAACG153900.021.3842963
TCAACGC154850.021.283514
CAACGCA155800.021.1235125
AACGCAG157000.021.083416
TATCAAC160800.020.7031732
CATGGGA57950.019.0928544
GAGTACT78450.019.08870512-13
AGAGTAC129550.018.50941810-11
ACGCAGA176700.018.3603277
CGCAGAG174450.018.3009858
AGTACTT84650.017.88499812-13
GTACTTT84500.017.66619714-15
GCAGAGT181000.016.8328049
CATGGGG46300.016.778744
TTCGCGA1401.073249E-515.62923564-65
Produced by FastQC (version 0.11.5)