FastQCFastQC Report
Fri 27 May 2016
SRR939590_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939590_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2717488
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32340.11900696525614833No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG78450.034.337291
GTATCAA112650.032.4007341
TACATGG87300.027.8075892
GGTATCA82300.025.7350541
ACATGGG90200.025.4998043
TATCAAC139950.024.0282152
GTATAGG7350.023.7804071
ATCAACG148100.022.6740443
TCAACGC150500.022.3719254
CAACGCA151050.022.3385755
GAGTACT66350.021.90806212-13
AACGCAG155200.021.8328746
CATGGGA53650.020.9487254
ACGCAGA163300.020.5187237
GTGTAGC16600.020.4391821
CGCAGAG170850.019.3910058
CATGGGG45200.019.014434
GTACTTT75250.019.00598314-15
AGAGTAC117100.018.61998210-11
GAGTACA73350.018.3623771
Produced by FastQC (version 0.11.5)