FastQCFastQC Report
Fri 27 May 2016
SRR939590_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939590_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2717488
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84260.3100657666197606No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63770.23466524967175567No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47770.17578734478312324No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT41950.15437050687988318No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG92100.025.9231361
TACATGG89400.025.537862
ACATGGG88650.024.6874353
GTATCAA135650.024.174731
GGTATCA97600.023.1570031
ATCAACG154950.020.8209843
TCAACGC155100.020.7704374
CAACGCA155550.020.7406735
CATGGGA55450.020.6700484
AACGCAG156650.020.6122386
TATCAAC159850.020.302642
GCGCGTA1201.1294105E-720.22019870-71
GAGTACT72950.019.80551112-13
AGAGTAC120900.019.26076710-11
AGTACTT81150.019.05312512-13
ACGCAGA172700.018.3417957
CGCAGAG171500.018.2502548
GTATAGG9500.017.8803311
CATGGGG40000.017.805484
GTACTTT81550.017.65908414-15
Produced by FastQC (version 0.11.5)