##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939590_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2717488 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.469174840882463 31.0 31.0 34.0 30.0 34.0 2 31.674615306488935 31.0 31.0 34.0 30.0 34.0 3 31.729535512208333 31.0 31.0 34.0 30.0 34.0 4 35.52883802982755 37.0 35.0 37.0 33.0 37.0 5 35.28539555648452 37.0 35.0 37.0 33.0 37.0 6 35.28763034096195 37.0 35.0 37.0 32.0 37.0 7 35.19954347544497 37.0 35.0 37.0 32.0 37.0 8 35.158592052660396 37.0 35.0 37.0 32.0 37.0 9 36.76354191812439 39.0 35.0 39.0 32.0 39.0 10-11 36.64056124626862 39.0 35.0 39.0 32.0 39.0 12-13 36.57945904452936 39.0 35.0 39.0 32.0 39.0 14-15 37.659275772330915 39.0 36.0 41.0 32.0 41.0 16-17 37.605156306118005 39.0 36.0 41.0 32.0 41.0 18-19 37.57297493126005 39.0 36.0 41.0 32.0 41.0 20-21 37.51134890016074 39.0 36.0 41.0 32.0 41.0 22-23 37.37955530990385 39.0 36.0 41.0 32.0 41.0 24-25 37.2558011295726 39.0 36.0 40.5 31.0 41.0 26-27 36.96841752383083 39.0 36.0 40.0 31.0 41.0 28-29 36.776650715660935 39.0 36.0 40.0 30.0 41.0 30-31 36.54420479501657 38.0 35.0 40.0 30.0 41.0 32-33 36.38906611547135 38.0 35.0 40.0 30.0 41.0 34-35 36.377456496588024 38.0 35.0 40.0 30.0 41.0 36-37 36.31382640880107 38.0 35.0 40.0 30.0 41.0 38-39 36.366663072661225 38.0 35.0 40.0 30.0 41.0 40-41 36.27461832398156 38.0 35.0 40.0 30.0 41.0 42-43 36.109626243059765 38.0 35.0 40.0 29.5 41.0 44-45 35.917803500880225 38.0 35.0 40.0 29.0 41.0 46-47 35.65393131450811 38.0 34.5 40.0 27.5 41.0 48-49 35.38725010009244 38.0 34.0 40.0 27.0 41.0 50-51 35.162524176739694 38.0 34.0 40.0 26.5 41.0 52-53 34.952876516842025 38.0 34.0 40.0 26.0 41.0 54-55 34.677872726576894 38.0 33.0 40.0 26.0 41.0 56-57 33.95256722384791 37.5 32.5 40.0 23.5 41.0 58-59 30.905867477611675 35.5 27.5 39.0 8.5 40.5 60-61 30.649814093015316 35.0 26.5 39.0 10.5 40.0 62-63 32.38831229429532 36.0 30.5 39.0 20.0 40.0 64-65 32.428959391909 36.0 31.0 39.0 19.0 40.0 66-67 27.58198030681276 32.0 19.0 37.0 5.0 39.5 68-69 19.95685151139582 20.5 8.0 32.5 2.0 37.0 70-71 17.981549136555525 16.0 7.5 29.0 2.0 35.5 72-73 19.7146651613549 20.0 7.5 31.0 2.0 36.0 74-75 21.206194286782498 24.0 9.5 32.5 2.0 36.0 76-77 23.129663498054086 27.5 12.5 33.0 2.0 36.0 78-79 25.359454209181422 31.0 17.5 35.0 2.0 37.0 80-81 26.956843783670802 32.5 23.5 35.0 2.0 37.0 82-83 27.99741066013907 33.5 27.0 35.0 2.0 36.5 84-85 28.005777946397558 34.0 28.0 35.0 2.0 36.0 86-87 27.71515495192619 34.0 27.0 35.0 2.0 36.0 88-89 27.270123179936768 33.0 26.0 35.0 2.0 35.5 90-91 25.471781660121405 32.5 16.5 34.5 2.0 35.0 92-93 23.91144119127665 31.5 4.5 34.0 2.0 35.0 94-95 20.08362999211036 28.5 2.0 34.0 2.0 35.0 96-97 17.35141995107246 19.0 2.0 33.0 2.0 35.0 98-99 17.614437855843338 22.0 2.0 33.0 2.0 35.0 100-101 16.816422372426302 18.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.2808407139498428 1101 2 0.24705807321722872 1101 3 0.26412837361261055 1101 4 0.23435468616315092 1101 5 0.32994576866596503 1101 6 0.32020644803341014 1101 7 0.35263191567956653 1101 8 0.37841013420192127 1101 9 0.3221611224876142 1101 10-11 0.3087214228621278 1101 12-13 0.32897796061721607 1101 14-15 0.4214407083589151 1101 16-17 0.365168567010393 1101 18-19 0.44262040040280226 1101 20-21 0.39330131394968504 1101 22-23 0.3454565345507703 1101 24-25 0.3793782472171827 1101 26-27 0.3693363930448683 1101 28-29 0.22939477627271998 1101 30-31 0.15359860975281947 1101 32-33 0.19510648870490144 1101 34-35 0.1559941273160348 1101 36-37 0.18952592660307488 1101 38-39 0.20680781800228942 1101 40-41 0.2036578563785696 1101 42-43 0.2384814338657506 1101 44-45 0.16374538760550195 1101 46-47 0.0861727731692028 1101 48-49 3.91159432474808E-4 1101 50-51 0.030181122270271032 1101 52-53 -0.010641987530917163 1101 54-55 0.025112324277465348 1101 56-57 -3.7162297675192164 1101 58-59 -10.096250796319932 1101 60-61 -11.927207802228349 1101 62-63 -4.117357061737017 1101 64-65 -1.216447494329806 1101 66-67 -2.3454990338892614 1101 68-69 -4.528491585648663 1101 70-71 -4.639775634182509 1101 72-73 -3.6795197618932356 1101 74-75 -6.419931282406932 1101 76-77 -4.959077968447442 1101 78-79 1.219270975623651 1101 80-81 1.815521280072936 1101 82-83 0.4423450450342905 1101 84-85 0.10766864186598113 1101 86-87 0.08344347436730715 1101 88-89 0.03603937224020015 1101 90-91 1.4199258723089017 1101 92-93 0.8036161611523944 1101 94-95 -8.648522776353435 1101 96-97 -9.513817477810544 1101 98-99 -8.775733474495294 1101 100-101 -9.725887799689707 1102 1 0.2393662370752132 1102 2 0.29103764572935376 1102 3 0.3280682081769264 1102 4 0.21175079133068664 1102 5 0.32451251910972445 1102 6 0.29564069264326776 1102 7 0.3287725841630049 1102 8 0.31750489219525235 1102 9 0.22648819340283666 1102 10-11 0.35644714756609375 1102 12-13 0.30912246808172483 1102 14-15 0.3475682485542677 1102 16-17 0.36298118236439336 1102 18-19 0.3622001025928867 1102 20-21 0.3128362708253434 1102 22-23 0.31506862413127834 1102 24-25 0.22990116394404225 1102 26-27 0.11153976274581368 1102 28-29 0.17968523563440186 1102 30-31 0.3388597471312025 1102 32-33 0.06666836980561897 1102 34-35 0.12458529188185707 1102 36-37 -0.14930459650624783 1102 38-39 -0.11464945407426086 1102 40-41 -0.09426864446723471 1102 42-43 0.02042249459291412 1102 44-45 -0.03613405041389228 1102 46-47 -0.1874509874273116 1102 48-49 -0.0731021528442497 1102 50-51 -0.030445247934615338 1102 52-53 -0.04396787401134361 1102 54-55 -0.2687316320557329 1102 56-57 -3.4418889905428323 1102 58-59 -9.626043215295283 1102 60-61 -7.852422204185075 1102 62-63 -2.358988050916615 1102 64-65 -0.8244946534517155 1102 66-67 -2.1339858783174463 1102 68-69 -5.106224725107033 1102 70-71 -4.867545654821846 1102 72-73 -3.4560846442040702 1102 74-75 -4.179480105836273 1102 76-77 -4.455066939401508 1102 78-79 1.3871263782833303 1102 80-81 1.9349689932150262 1102 82-83 0.5702597015321551 1102 84-85 0.17483302793394628 1102 86-87 0.16113706846888576 1102 88-89 -0.03559081447680157 1102 90-91 1.3840437251757791 1102 92-93 0.9131581476004023 1102 94-95 -9.267450365338512 1102 96-97 -10.103879652612576 1102 98-99 -9.20805602594117 1102 100-101 -10.05220478525501 1103 1 0.13588622909987436 1103 2 0.15970469071332616 1103 3 0.19560275519307524 1103 4 0.07092534386822535 1103 5 0.13039095655700805 1103 6 0.151122050291157 1103 7 0.11995002009189903 1103 8 0.24092753908717413 1103 9 0.3149874495492142 1103 10-11 0.2927545661639712 1103 12-13 0.23287363754231905 1103 14-15 0.3216023891349309 1103 16-17 0.3788959689474183 1103 18-19 0.26309149996409076 1103 20-21 0.240033092959969 1103 22-23 0.2602884827224088 1103 24-25 0.23992002868538265 1103 26-27 0.2942458562106367 1103 28-29 0.2952273285470923 1103 30-31 0.21410644580146965 1103 32-33 0.13260662228640996 1103 34-35 0.0780176012022622 1103 36-37 0.14576449752605214 1103 38-39 0.4026435322296251 1103 40-41 0.3442724822026051 1103 42-43 0.22283196684026763 1103 44-45 0.2401629388960842 1103 46-47 0.17372322769209347 1103 48-49 0.1702189618625809 1103 50-51 0.07750248292840922 1103 52-53 0.24579271248272505 1103 54-55 0.23025059214328536 1103 56-57 0.5689451991168397 1103 58-59 -8.178108981157767 1103 60-61 -7.476904650773317 1103 62-63 -2.2891063006367105 1103 64-65 -0.4104930277097836 1103 66-67 -1.9919300816272099 1103 68-69 -3.529441240191158 1103 70-71 -4.141231641233098 1103 72-73 -3.04059561796214 1103 74-75 -3.7431999874331794 1103 76-77 -0.5444873470639671 1103 78-79 3.260021886020944 1103 80-81 2.7909840850080982 1103 82-83 1.0748047238477625 1103 84-85 0.721995494767274 1103 86-87 0.6465691937430371 1103 88-89 0.5810721201407709 1103 90-91 1.8148846631981996 1103 92-93 1.290793153198475 1103 94-95 -7.307245460505765 1103 96-97 -8.778862244904106 1103 98-99 -7.804063755883963 1103 100-101 -9.143913194635234 1104 1 0.2189366800406667 1104 2 0.18788825154616973 1104 3 0.27556095544038683 1104 4 0.15781810160154208 1104 5 0.31460540289528893 1104 6 0.20152357618504624 1104 7 0.2882319810278702 1104 8 0.23024520765881817 1104 9 0.29298445123457384 1104 10-11 0.247752274458513 1104 12-13 0.3741261858710416 1104 14-15 0.3902621830724158 1104 16-17 0.3204692366806867 1104 18-19 0.3088493383129176 1104 20-21 0.31087382659218576 1104 22-23 0.3861188081923288 1104 24-25 0.460746260118988 1104 26-27 0.4822638174521643 1104 28-29 0.5163259387232202 1104 30-31 0.32188230984604616 1104 32-33 0.5887489300338089 1104 34-35 0.49323786751753573 1104 36-37 0.3785459117947809 1104 38-39 0.31244725720588207 1104 40-41 0.2552326232379798 1104 42-43 0.33731100967128924 1104 44-45 0.42876051064317977 1104 46-47 0.4455725504453838 1104 48-49 0.28340629446066856 1104 50-51 0.3643114501808853 1104 52-53 0.2434046240992842 1104 54-55 0.3380121330287338 1104 56-57 0.7391100586782642 1104 58-59 -8.366177908973821 1104 60-61 -7.865639128049626 1104 62-63 -2.0904383544344967 1104 64-65 -0.31843301128589374 1104 66-67 -1.5601845301617097 1104 68-69 -3.348309734492929 1104 70-71 -3.6943944931650528 1104 72-73 -2.637766913396394 1104 74-75 -3.317276919507986 1104 76-77 -0.01578636210805584 1104 78-79 3.891772152192985 1104 80-81 3.281770414507598 1104 82-83 1.705087403328882 1104 84-85 1.3653922200156643 1104 86-87 1.385424045235748 1104 88-89 1.3433045754644475 1104 90-91 2.9670821342057394 1104 92-93 2.850997807270314 1104 94-95 -5.89556617752311 1104 96-97 -7.731641116812094 1104 98-99 -5.463373988348835 1104 100-101 -4.929963750612528 1105 1 0.15345407305608205 1105 2 0.29633223400201203 1105 3 0.2345330878314904 1105 4 0.22241594337329218 1105 5 0.3210390219771284 1105 6 0.2384555726032076 1105 7 0.26062744897669887 1105 8 0.2673290614422754 1105 9 0.34490541125830276 1105 10-11 0.3122702663480439 1105 12-13 0.2779694605147114 1105 14-15 0.41922951021168586 1105 16-17 0.4520094410963864 1105 18-19 0.37424710479763945 1105 20-21 0.30676476591197144 1105 22-23 0.5016227322436819 1105 24-25 0.453981121436847 1105 26-27 0.39215465125604254 1105 28-29 0.4795543550148764 1105 30-31 0.5557986186141335 1105 32-33 0.48713033014445983 1105 34-35 0.5725672996137661 1105 36-37 0.36396332705410117 1105 38-39 0.39446021570105216 1105 40-41 0.41716426318345157 1105 42-43 0.333258628148549 1105 44-45 0.5549432870235833 1105 46-47 0.3540264428419633 1105 48-49 0.24375512437470803 1105 50-51 0.33124575654207433 1105 52-53 0.44827981588159105 1105 54-55 0.4496526060822603 1105 56-57 0.986611898077328 1105 58-59 -8.486733407493745 1105 60-61 -7.7694749167668355 1105 62-63 -1.8514975631221624 1105 64-65 0.2095162298550477 1105 66-67 -1.5123205858056679 1105 68-69 -3.1692912891701326 1105 70-71 -3.8318350335206137 1105 72-73 -2.6397367814729833 1105 74-75 -3.3168088708680017 1105 76-77 0.16726521521229287 1105 78-79 4.1174614277834465 1105 80-81 3.548306113108513 1105 82-83 2.1573467985230614 1105 84-85 1.6688556373624799 1105 86-87 1.510699598623951 1105 88-89 1.3707601019318325 1105 90-91 2.850812506819512 1105 92-93 2.4257836384790714 1105 94-95 -6.204602688881755 1105 96-97 -7.794196013606122 1105 98-99 -5.72867201377532 1105 100-101 -5.06219586851658 1106 1 0.08786519968688822 1106 2 0.2243456139604696 1106 3 0.17290503878925634 1106 4 0.14195457915906928 1106 5 0.15419404391933966 1106 6 0.20217085554045866 1106 7 0.16348571453519156 1106 8 0.22441710434267748 1106 9 0.09212589989755315 1106 10-11 0.13990258825953816 1106 12-13 0.2075488437554256 1106 14-15 0.10808851543223597 1106 16-17 0.2121599821393687 1106 18-19 0.1881213820586467 1106 20-21 0.2397287635212848 1106 22-23 0.21614454401949246 1106 24-25 0.21942239259894336 1106 26-27 0.16476552626966878 1106 28-29 0.22391360623911538 1106 30-31 0.297015131569772 1106 32-33 0.20374069664040206 1106 34-35 0.24313948105551475 1106 36-37 0.29650713717227717 1106 38-39 0.37932933844202665 1106 40-41 0.32392061387572824 1106 42-43 0.32873067453592597 1106 44-45 0.2695038980372786 1106 46-47 0.16513359147771922 1106 48-49 0.12294833137111993 1106 50-51 0.24020210657393903 1106 52-53 0.25263094403135256 1106 54-55 0.1819543749972965 1106 56-57 0.8476355045242769 1106 58-59 -8.729179938841877 1106 60-61 -7.9513673949979875 1106 62-63 -2.168810938787942 1106 64-65 -0.16503427684649097 1106 66-67 0.7085205486510233 1106 68-69 -2.1260210582568817 1106 70-71 -2.2701714873032905 1106 72-73 -0.8688793018054355 1106 74-75 -0.8079263219396857 1106 76-77 2.5033731068121767 1106 78-79 4.681357168452639 1106 80-81 3.6776891887158456 1106 82-83 1.9541617938506413 1106 84-85 1.5631813726493142 1106 86-87 1.4690952583926062 1106 88-89 1.3744930839256817 1106 90-91 2.7438471355520804 1106 92-93 2.4532964257172587 1106 94-95 -5.85114080624367 1106 96-97 -7.398977070332258 1106 98-99 -5.19045641549107 1106 100-101 -4.595856271170119 1107 1 0.02648852295284243 1107 2 0.03219035121655622 1107 3 0.07901069200831756 1107 4 0.05598380982781492 1107 5 0.09983225850633914 1107 6 0.04697181854996302 1107 7 0.0018849366207547291 1107 8 0.07480035783920158 1107 9 0.040867354660122146 1107 10-11 0.012379150091142321 1107 12-13 -0.005302429785096763 1107 14-15 0.04754025870207812 1107 16-17 0.04754197989652198 1107 18-19 0.0566316703819254 1107 20-21 0.026651525890322603 1107 22-23 0.07383385477957205 1107 24-25 0.05637015476098384 1107 26-27 0.1501795154822645 1107 28-29 0.09104304122999451 1107 30-31 0.059910378987225954 1107 32-33 0.10570539978454008 1107 34-35 0.12861386535224284 1107 36-37 0.07486166321576349 1107 38-39 0.04266250771662072 1107 40-41 -0.11325642421687121 1107 42-43 -0.06586426442041926 1107 44-45 -0.11958418678998584 1107 46-47 -0.10352782254921777 1107 48-49 -0.3316218469806742 1107 50-51 -0.16277144286309664 1107 52-53 -0.08378945359184797 1107 54-55 -0.166155553255372 1107 56-57 0.3230769452115254 1107 58-59 -8.801510526721096 1107 60-61 -7.894716300112847 1107 62-63 -2.549199463629961 1107 64-65 -0.7359290440471256 1107 66-67 0.358093827253132 1107 68-69 -2.8156207993813815 1107 70-71 -2.5656045287960474 1107 72-73 -1.3025162358142595 1107 74-75 -1.2561079837290556 1107 76-77 1.7909724965136427 1107 78-79 3.914302916831911 1107 80-81 2.8409005788699275 1107 82-83 1.1663903724548668 1107 84-85 0.7948047998702492 1107 86-87 0.6046197802686919 1107 88-89 0.5203741207892243 1107 90-91 1.9392982541720727 1107 92-93 1.3788356806820268 1107 94-95 -6.9494596794084735 1107 96-97 -8.16791757588465 1107 98-99 -6.209690156886037 1107 100-101 -5.447954436870679 1108 1 -0.020530194747379937 1108 2 -0.028497939042839704 1108 3 -0.09240844421305283 1108 4 -0.07717218916446456 1108 5 -0.12453581768085087 1108 6 -0.01661945151646904 1108 7 -0.061016250172677644 1108 8 -0.05107067228115625 1108 9 -0.1696621744581961 1108 10-11 -0.11907524494834121 1108 12-13 -0.0563463495397869 1108 14-15 -0.07197987184746779 1108 16-17 -0.042595083510342135 1108 18-19 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2312 98-99 -1.2763789268692953 2312 100-101 -0.44567194116550546 2313 1 -0.2717578997712593 2313 2 -0.28829356218520985 2313 3 -0.21724124040557413 2313 4 -0.1587714612319644 2313 5 -0.18103939877095598 2313 6 -0.1158983297594034 2313 7 -0.23020607110455416 2313 8 -0.33311400496974386 2313 9 -0.25947575072628837 2313 10-11 -0.30531018298417933 2313 12-13 -0.2849487572746128 2313 14-15 -0.30319161248337423 2313 16-17 -0.31169936147595223 2313 18-19 -0.3419767741852482 2313 20-21 -0.3167815826923217 2313 22-23 -0.20809234059915838 2313 24-25 -0.2329195431627724 2313 26-27 -0.39452425715497697 2313 28-29 -0.30116283504768404 2313 30-31 -0.41367974705725175 2313 32-33 -0.3557731930919985 2313 34-35 -0.382624738569767 2313 36-37 -0.495917031635706 2313 38-39 -0.4477653847074521 2313 40-41 -0.3699196373618463 2313 42-43 -0.2348179017071459 2313 44-45 -0.26601267279941254 2313 46-47 -0.44498111738284507 2313 48-49 -0.4250677839649555 2313 50-51 -0.5152008486036905 2313 52-53 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-4.248110949585914 2314 76-77 0.33894640954194344 2314 78-79 3.2012969187835445 2314 80-81 2.314645370291114 2314 82-83 0.7300770861493042 2314 84-85 0.26937746510897753 2314 86-87 0.4844977768926064 2314 88-89 0.6596199220680852 2314 90-91 2.24690749249325 2314 92-93 3.828176742001421 2314 94-95 6.346371013631451 2314 96-97 3.259125007465702 2314 98-99 5.483775924290111 2314 100-101 5.94722831507441 2315 1 -0.4172866351776605 2315 2 -0.3842785740002448 2315 3 -0.5050323359414506 2315 4 -0.32144752542123456 2315 5 -0.3763028732458622 2315 6 -0.3703364665993263 2315 7 -0.4950568342904873 2315 8 -0.6074802906849399 2315 9 -0.5745204758482174 2315 10-11 -0.4894460237628451 2315 12-13 -0.5585858076941079 2315 14-15 -0.7018714927350445 2315 16-17 -0.6991270073296434 2315 18-19 -0.6332943297180336 2315 20-21 -0.6846777101959489 2315 22-23 -0.7471664198838539 2315 24-25 -0.6040939910468168 2315 26-27 -0.6047690149799436 2315 28-29 -0.538833285038308 2315 30-31 -0.7590759183595992 2315 32-33 -0.657789154935621 2315 34-35 -0.6313179707926722 2315 36-37 -0.5995083137275472 2315 38-39 -0.6849853184837968 2315 40-41 -0.6895382753333763 2315 42-43 -0.6956669490453251 2315 44-45 -0.587161186536548 2315 46-47 -0.5771689241397411 2315 48-49 -0.6599958505517307 2315 50-51 -0.5154350257214375 2315 52-53 -0.5198079130971323 2315 54-55 -0.4930065237991528 2315 56-57 0.3141080896557398 2315 58-59 3.370699461208755 2315 60-61 3.513862464821699 2315 62-63 0.7195020860290526 2315 64-65 -0.0633705299010785 2315 66-67 -1.9384652724877292 2315 68-69 -5.104256069349876 2315 70-71 -3.8118184526837595 2315 72-73 -3.238708626708526 2315 74-75 -0.7892913947657476 2315 76-77 1.8209316530448127 2315 78-79 3.4844928569853586 2315 80-81 2.460215853119813 2315 82-83 0.8368360017017018 2315 84-85 0.6018233350074844 2315 86-87 0.4939107925825468 2315 88-89 0.6406158865256479 2315 90-91 2.3340151960402906 2315 92-93 3.9632566472761432 2315 94-95 6.608689796762814 2315 96-97 4.278846813758815 2315 98-99 6.5474914513678435 2315 100-101 6.65397239783457 2316 1 -0.4174557179118672 2316 2 -0.3779941000732414 2316 3 -0.4878049818503598 2316 4 -0.29922006143173263 2316 5 -0.4771719284786613 2316 6 -0.6018291712207926 2316 7 -0.4333195494032367 2316 8 -0.4781566591480626 2316 9 -0.6549860591488113 2316 10-11 -0.6282224737652271 2316 12-13 -0.5595272886637161 2316 14-15 -0.7082705826689377 2316 16-17 -0.7348469947422487 2316 18-19 -0.6308547993112867 2316 20-21 -0.6699202291963147 2316 22-23 -0.8802536701129 2316 24-25 -0.6754437955616552 2316 26-27 -0.8127903127569382 2316 28-29 -0.725092659239202 2316 30-31 -0.6411249502081517 2316 32-33 -0.645916480560345 2316 34-35 -0.7269289496980704 2316 36-37 -0.5440526078821861 2316 38-39 -0.6940748824765777 2316 40-41 -0.6866782360594357 2316 42-43 -0.7228710421984417 2316 44-45 -0.4655085180689795 2316 46-47 -0.5873040241392573 2316 48-49 -0.5382161287930813 2316 50-51 -0.5633272009005879 2316 52-53 -0.42150876531914605 2316 54-55 -0.33210687928032456 2316 56-57 0.4323447936896514 2316 58-59 3.622785842436169 2316 60-61 3.4759538370454948 2316 62-63 0.9429484574781952 2316 64-65 0.37781489338741636 2316 66-67 -1.8926077726713402 2316 68-69 -3.051059499681518 2316 70-71 -3.2311137733164124 2316 72-73 -0.6525794687287494 2316 74-75 5.622860046324146 2316 76-77 5.749529633379968 2316 78-79 4.827653020792969 2316 80-81 3.197094330667568 2316 82-83 1.4870458540679188 2316 84-85 1.0948183027321967 2316 86-87 0.8097134879350207 2316 88-89 0.8136066620278406 2316 90-91 2.361174043211264 2316 92-93 4.144449490047965 2316 94-95 6.494952441111703 2316 96-97 3.798469738890116 2316 98-99 6.215294968009946 2316 100-101 6.251118201939841 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 12.0 4 40.0 5 99.0 6 223.0 7 540.0 8 1272.0 9 2247.0 10 3611.0 11 5480.0 12 8094.0 13 11226.0 14 14141.0 15 18819.0 16 25309.0 17 33604.0 18 36984.0 19 37617.0 20 39696.0 21 40749.0 22 41287.0 23 41808.0 24 44729.0 25 47617.0 26 59535.0 27 83740.0 28 116748.0 29 153799.0 30 188254.0 31 213935.0 32 225535.0 33 225416.0 34 229450.0 35 244581.0 36 248801.0 37 191460.0 38 76183.0 39 4844.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.67255597418716 20.298565806386073 14.483463560645882 23.545414658780885 2 15.342231925256538 21.59186849084366 39.75747975624316 23.30841982765664 3 18.91648224348864 25.607345329242293 30.44428629150073 25.03188613576833 4 12.482226232461745 17.69270002296238 38.42276396436709 31.402309780208782 5 12.82548358139271 38.47405741486559 35.194496668117395 13.505962335624302 6 30.632591569861578 38.10750958237902 17.80147695224413 13.458421895515272 7 27.26216270320237 33.07617181750205 21.898495963919622 17.763169515375964 8 25.257204636932812 36.33490660117476 20.158779106865683 18.24910965502674 9 25.667160259769318 16.677129761014584 20.644838174078416 37.01087180513768 10-11 24.12993176050823 27.140653426988454 28.49477164204589 20.234643170457424 12-13 25.19076441183917 24.476667422266445 28.623033477976715 21.709534687917664 14-15 22.129274264430837 25.895938888757026 26.773961299671882 25.200825547140255 16-17 21.53804627916275 29.013814788040342 27.342877324321574 22.10526160847533 18-19 21.377327777449658 28.10433344575368 29.249622674246485 21.268716102550176 20-21 22.335738005098825 27.23651401588526 29.090100857851077 21.337647121164842 22-23 21.812239833257774 27.21958661822978 28.946880354209476 22.021293194302974 24-25 21.738917642002242 27.358105394200184 29.130002077289525 21.772974886508052 26-27 21.870289184828536 27.350745643901792 29.150830170633974 21.628135000635698 28-29 21.604937703717535 27.603927568324437 29.11572674089659 21.675407987061433 30-31 21.875952212371143 27.378838105864673 29.007756068766845 21.73745361299734 32-33 21.79657948770885 27.368385607771415 28.991074548414325 21.843960356105406 34-35 22.04282121616022 27.374755192206777 28.93365000415833 21.648773587474672 36-37 22.018788896080995 27.184308150417973 29.11232916569604 21.68457378780499 38-39 21.76404742864077 27.58831368249592 29.030625181490432 21.61701370737288 40-41 21.949008392163925 27.421602331899376 29.005954048146894 21.623435227789805 42-43 21.760259994630097 27.445303784381835 28.808294105666278 21.98614211532179 44-45 21.95291062779691 27.40004788125449 28.738973499567226 21.908067991381376 46-47 21.998718680362312 27.53071217100811 28.533006871210066 21.937562277419513 48-49 21.893382278475418 27.60610601450015 28.528246632842567 21.97226507418187 50-51 21.829720001539272 27.729460700402143 28.44479894357296 21.996020354485626 52-53 21.995380482409306 27.895980217006127 28.14463000410918 21.964009296475382 54-55 21.910926046602384 27.603334842811755 28.265112272059557 22.220626838526307 56-57 21.877219773247575 27.797333160507858 28.303952388545056 22.02149467769951 58-59 21.260319830661096 27.297966306050565 29.677895450425435 21.763818412862907 60-61 21.42508883128545 27.52559709005195 29.276690785158348 21.772623293504253 62-63 22.005180326095218 27.54964172508502 28.43028244129997 22.01489550751979 64-65 21.782908195277084 27.666792643493064 28.4077130201282 22.14258614110165 66-67 21.88318603432776 27.24524316987063 28.851250081860343 22.020320713941263 68-69 21.462960631417214 26.630770355839395 30.29505139047944 21.611217622263958 70-71 22.376467347404443 26.545570132804347 29.378489663539582 21.699472856251628 72-73 21.56504724325493 26.77287053063892 29.67895424160536 21.983127984500786 74-75 21.52079764050858 27.209246063165303 29.23657987066337 22.03337642566275 76-77 20.401404377743503 27.51343946494246 29.525503470162533 22.5596526871515 78-79 21.661903365274746 27.403752476099452 28.64805201173539 22.286292146890407 80-81 21.600966117062733 27.7308618151471 28.12323183165031 22.54494023613986 82-83 21.85009702907229 27.79213644088862 27.98312189085163 22.374644639187455 84-85 21.88045951209729 27.6619511508502 27.852092818720507 22.605496518332004 86-87 21.58389193085933 27.680371142677927 28.062660318302612 22.673076608160127 88-89 21.728379615361614 27.607523493668324 27.950708144931667 22.71338874603839 90-91 21.72618355326096 27.430419020001175 28.273668757575994 22.569728669161876 92-93 21.537827967848884 27.60659892823473 28.164223202140366 22.69134990177602 94-95 20.55521334039725 27.422226961221035 29.013131154764388 23.009428543617325 96-97 20.372981036254675 27.372236211718757 29.102695044961695 23.15208770706487 98-99 20.97113649751676 27.226244133568073 28.549169324535562 23.253450044379605 100-101 20.192298262335896 27.492478547739363 28.975137712105507 23.340085477819233 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 486.0 1 365.5 2 281.5 3 341.0 4 350.5 5 356.5 6 481.0 7 746.0 8 1102.0 9 1407.5 10 1544.0 11 1707.0 12 1857.0 13 1997.5 14 2232.0 15 2456.5 16 2758.0 17 3050.5 18 3336.5 19 3740.5 20 4386.5 21 5231.0 22 6264.5 23 7668.5 24 9492.5 25 11960.0 26 15340.0 27 19531.5 28 24167.0 29 29401.5 30 34975.5 31 41019.0 32 47901.0 33 55324.5 34 62840.0 35 70266.0 36 77615.0 37 83745.5 38 88156.5 39 91768.5 40 94483.0 41 96781.0 42 99283.5 43 101609.5 44 103925.0 45 106011.5 46 107716.0 47 110074.0 48 111409.5 49 108406.5 50 102327.5 51 96200.0 52 90401.0 53 84620.5 54 78056.5 55 70631.5 56 63250.5 57 55896.0 58 48832.0 59 41514.0 60 33929.0 61 26599.0 62 19980.0 63 14783.5 64 10679.0 65 7518.0 66 5280.0 67 3606.5 68 2365.5 69 1479.0 70 937.5 71 599.0 72 367.5 73 219.0 74 139.5 75 85.0 76 43.0 77 20.0 78 10.5 79 3.5 80 2.5 81 2.0 82 1.0 83 0.0 84 0.0 85 0.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025759083388776693 2 0.013026736456609928 3 2.2079214333237167E-4 4 0.0 5 0.05909869703196481 6 0.0 7 0.0 8 7.359738111079056E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 6.439770847194174E-4 16-17 5.519803583309292E-5 18-19 3.127888697208598E-4 20-21 0.0 22-23 0.0 24-25 1.6559410749927874E-4 26-27 1.6559410749927874E-4 28-29 0.001122360061939556 30-31 0.004857427153312176 32-33 0.005391008166365408 34-35 0.001839934527769764 36-37 0.08539136143379474 38-39 0.07817881808493726 40-41 0.08377221904935735 42-43 0.08666091625795588 44-45 0.08971520757405368 46-47 0.08531776405268394 48-49 0.07606289337800204 50-51 0.07063508652108123 52-53 0.05961387869974035 54-55 0.048500674152010974 56-57 0.0676727919313719 58-59 0.7163417096966022 60-61 0.3357328532821488 62-63 0.0033670801858186675 64-65 0.0020975253616575306 66-67 1.103371937612972 68-69 2.3794769286929696 70-71 2.3495043952356 72-73 1.8113603445534994 74-75 1.2833911318099656 76-77 0.7436279387434278 78-79 0.5669022273511419 80-81 0.038528229011498855 82-83 1.2879541694388346E-4 84-85 0.0014903469674935087 86-87 0.0015639443486042991 88-89 1.839934527769764E-5 90-91 0.33475768798243083 92-93 0.05525323386892601 94-95 0.9521293194302973 96-97 1.6576522141036134 98-99 0.6434803023969194 100-101 0.5660926561589232 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2717488.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.496180502295424 #Duplication Level Percentage of deduplicated Percentage of total 1 74.67372153764042 41.44106329231037 2 13.985140925512752 15.522438103045891 3 4.8784504086476606 8.122060933494224 4 2.2290264461678126 4.948098160036761 5 1.2218545657890263 3.39041307652908 6 0.740930431862194 2.4671285391760813 7 0.4501732409674434 1.7488026806622796 8 0.31515696504758495 1.3992006255069107 9 0.24193600859682968 1.2083871962785102 >10 1.147797209031119 11.231363083053049 >50 0.0741649839004492 2.8458466716555124 >100 0.039524668959824455 3.914211644604097 >500 0.0013929614192299575 0.5239005113557195 >1k 5.969834653559381E-4 0.6921656348129949 >5k 1.326629923013196E-4 0.5449198474784217 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8426 0.3100657666197606 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6377 0.23466524967175567 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4777 0.17578734478312324 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4195 0.15437050687988318 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 3.679869055539528E-5 0.0 3 0.0 0.0 0.0 3.679869055539528E-5 0.0 4 0.0 0.0 0.0 7.359738111079056E-5 0.0 5 0.0 0.0 0.0 7.359738111079056E-5 0.0 6 0.0 0.0 0.0 7.359738111079056E-5 0.0 7 0.0 0.0 0.0 7.359738111079056E-5 0.0 8 0.0 0.0 0.0 7.359738111079056E-5 0.0 9 0.0 0.0 0.0 1.1039607166618584E-4 0.0 10-11 0.0 0.0 0.0 1.1039607166618584E-4 0.0 12-13 0.0 0.0 0.0 1.839934527769764E-4 0.0 14-15 0.0 0.0 0.0 2.391914886100693E-4 0.0 16-17 0.0 0.0 0.0 4.0478559610934804E-4 0.0 18-19 0.0 0.0 0.0 4.783829772201386E-4 0.0 20-21 0.0 0.0 0.0 5.151816677755338E-4 0.0 22-23 0.0 0.0 0.0 9.199672638848819E-4 0.0 24-25 0.0 0.0 0.0 0.0015271456580489041 0.0 26-27 0.0 0.0 0.0 0.002115924706935228 0.0 28-29 0.0 0.0 0.0 0.0035326742933179467 0.0 30-31 0.0 0.0 0.0 0.007396536801634451 0.0 32-33 0.0 0.0 0.0 0.015878634974653062 0.0 34-35 0.0 0.0 0.0 0.030211724945979526 0.0 36-37 0.0 0.0 0.0 0.0521621438622728 0.0 38-39 0.0 0.0 0.0 0.0858145463751818 0.0 40-41 0.0 0.0 0.0 0.12357000288501734 0.0 42-43 0.0 0.0 0.0 0.16320219261317806 0.0 44-45 0.0 0.0 0.0 0.21245723992157464 0.0 46-47 0.0 0.0 0.0 0.2692744181391049 0.0 48-49 0.0 0.0 0.0 0.33041544249689414 0.0 50-51 0.0 0.0 0.0 0.39113328191329644 0.0 52-53 1.839934527769764E-5 0.0 0.0 0.45093115406581374 0.0 54-55 3.679869055539528E-5 0.0 0.0 0.5213086497530073 0.0 56-57 7.359738111079056E-5 0.0 0.0 0.5894966233521546 0.0 58-59 7.359738111079056E-5 0.0 0.0 0.640407611735544 0.0 60-61 7.359738111079056E-5 0.0 0.0 0.6837564692097997 0.0 62-63 1.1039607166618584E-4 0.0 0.0 0.7215119257196352 0.0 64-65 1.2879541694388346E-4 0.0 0.0 0.7644191989072261 0.0 66-67 1.471947622215811E-4 0.0 0.0 0.8021010580359509 0.0 68-69 1.471947622215811E-4 0.0 0.0 0.8508225243312941 0.0 70-71 1.471947622215811E-4 0.0 0.0 0.9245119021684732 0.0 72-73 1.471947622215811E-4 0.0 0.0 1.0248803306583139 0.0 74-75 1.471947622215811E-4 0.0 0.0 1.1573004186219038 0.0 76-77 1.471947622215811E-4 0.0 0.0 1.3262984049975564 0.0 78-79 1.471947622215811E-4 0.0 0.0 1.5486544926785326 0.0 80-81 1.471947622215811E-4 0.0 0.0 1.8116731334232203 0.0 82-83 1.471947622215811E-4 1.839934527769764E-5 0.0 2.0754829460148487 0.0 84-85 1.471947622215811E-4 3.679869055539528E-5 0.0 2.309099433005776 0.0 86-87 1.471947622215811E-4 3.679869055539528E-5 0.0 2.5291924012175953 0.0 88-89 1.471947622215811E-4 3.679869055539528E-5 0.0 2.7452007147777655 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 9210 0.0 25.923136 1 TACATGG 8940 0.0 25.53786 2 ACATGGG 8865 0.0 24.687435 3 GTATCAA 13565 0.0 24.17473 1 GGTATCA 9760 0.0 23.157003 1 ATCAACG 15495 0.0 20.820984 3 TCAACGC 15510 0.0 20.770437 4 CAACGCA 15555 0.0 20.740673 5 CATGGGA 5545 0.0 20.670048 4 AACGCAG 15665 0.0 20.612238 6 TATCAAC 15985 0.0 20.30264 2 GCGCGTA 120 1.1294105E-7 20.220198 70-71 GAGTACT 7295 0.0 19.805511 12-13 AGAGTAC 12090 0.0 19.260767 10-11 AGTACTT 8115 0.0 19.053125 12-13 ACGCAGA 17270 0.0 18.341795 7 CGCAGAG 17150 0.0 18.250254 8 GTATAGG 950 0.0 17.880331 1 CATGGGG 4000 0.0 17.80548 4 GTACTTT 8155 0.0 17.659084 14-15 >>END_MODULE