##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939589_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 982632 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.465554754984574 31.0 31.0 34.0 30.0 34.0 2 31.6164515301761 31.0 31.0 34.0 30.0 34.0 3 31.649179957501893 31.0 31.0 34.0 30.0 34.0 4 35.37206604303544 37.0 35.0 37.0 33.0 37.0 5 35.16621990735087 37.0 35.0 37.0 32.0 37.0 6 35.25401574546728 37.0 35.0 37.0 32.0 37.0 7 35.20302819366762 37.0 35.0 37.0 32.0 37.0 8 35.2055632220404 37.0 35.0 37.0 32.0 37.0 9 36.72343563002222 39.0 35.0 39.0 32.0 39.0 10-11 36.60931203136067 39.0 35.0 39.0 32.0 39.0 12-13 36.57479961979662 39.0 35.0 39.0 32.0 39.0 14-15 37.315527074225145 39.0 36.0 41.0 32.0 41.0 16-17 37.33964342704084 39.0 36.0 40.5 32.0 41.0 18-19 37.51895012578463 39.0 36.0 41.0 32.0 41.0 20-21 37.48053849253841 39.0 36.0 41.0 32.0 41.0 22-23 37.34153935552679 39.0 36.0 41.0 31.5 41.0 24-25 37.15373049117065 39.0 36.0 40.5 31.0 41.0 26-27 36.67375782592059 38.5 35.5 40.0 30.5 41.0 28-29 36.43174657450602 38.0 35.0 40.0 30.0 41.0 30-31 36.20291116104503 38.0 35.0 40.0 30.0 41.0 32-33 35.95376448151495 38.0 35.0 40.0 30.0 41.0 34-35 35.8686018774068 38.0 35.0 40.0 30.0 41.0 36-37 35.57717029366029 38.0 34.5 40.0 29.0 41.0 38-39 35.40030855905364 38.0 34.0 40.0 28.0 41.0 40-41 35.23105750677772 38.0 34.0 40.0 27.0 41.0 42-43 35.06354464336598 38.0 34.0 40.0 26.5 41.0 44-45 34.78515710866326 38.0 34.0 40.0 25.5 41.0 46-47 34.27817789365703 38.0 33.0 40.0 23.5 41.0 48-49 33.85252770111293 38.0 33.0 40.0 22.5 41.0 50-51 33.55144601437771 38.0 33.0 40.0 20.0 41.0 52-53 33.154206254223354 37.0 32.5 40.0 18.5 41.0 54-55 32.92643532879043 37.0 32.0 40.0 15.5 41.0 56-57 31.152649720342914 36.0 29.0 39.5 6.5 40.5 58-59 26.62376505141294 32.5 15.5 38.0 2.0 40.0 60-61 27.271394581084273 32.0 18.0 38.0 2.0 40.0 62-63 29.965626501070595 34.5 28.5 38.0 2.0 40.0 64-65 29.982681207206767 35.0 28.5 38.0 2.0 40.0 66-67 23.663659437103618 26.0 16.0 35.5 2.0 38.5 68-69 16.69958692572601 13.0 7.0 28.0 2.0 35.0 70-71 15.633619707072434 12.0 7.0 26.0 2.0 33.5 72-73 16.737434767033847 13.0 7.0 28.5 2.0 34.0 74-75 17.688843839809817 17.0 7.5 29.0 2.0 34.5 76-77 18.71911305554877 21.0 7.0 30.0 2.0 34.0 78-79 21.626948338747365 27.0 9.0 33.0 2.0 35.5 80-81 24.925976866212377 30.5 18.0 34.0 2.0 36.0 82-83 26.054523463514315 32.5 24.0 35.0 2.0 36.0 84-85 26.08713689356748 33.0 24.0 35.0 2.0 36.0 86-87 25.82104999633637 33.0 22.5 35.0 2.0 35.0 88-89 25.26345111903541 32.5 19.0 35.0 2.0 35.0 90-91 24.024687268478942 31.5 4.5 34.5 2.0 35.0 92-93 21.925926491300913 30.5 2.0 34.0 2.0 35.0 94-95 15.255919306515562 4.5 2.0 31.5 2.0 34.5 96-97 12.846780381668825 2.0 2.0 29.5 2.0 34.0 98-99 12.558840440775388 2.0 2.0 29.0 2.0 34.0 100-101 12.269919461202159 2.0 2.0 30.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.57466580667624 1101 2 0.5199341406196396 1101 3 0.5970141917355747 1101 4 0.30484203969903945 1101 5 0.5096543065213766 1101 6 0.37054491816471113 1101 7 0.563849179952733 1101 8 0.5831721665373664 1101 9 0.5541132742733765 1101 10-11 0.6667407661551223 1101 12-13 0.7164431850857582 1101 14-15 1.005495767376111 1101 16-17 0.8196076410986848 1101 18-19 0.6824032899810675 1101 20-21 0.8566799112392474 1101 22-23 0.6936165420975158 1101 24-25 0.7242790072763015 1101 26-27 0.8902797965701055 1101 28-29 0.7569874274658517 1101 30-31 0.9801181156740455 1101 32-33 0.8995294328002146 1101 34-35 0.8272106289594845 1101 36-37 0.679141437380288 1101 38-39 0.8692908375067816 1101 40-41 0.8553695092694227 1101 42-43 0.5991308976941951 1101 44-45 0.4235535248738529 1101 46-47 0.7797248034131243 1101 48-49 0.5596269702882921 1101 50-51 0.3430153791986328 1101 52-53 0.2807374856549174 1101 54-55 0.11109508693504466 1101 56-57 -1.5922528072793938 1101 58-59 -9.272940828351146 1101 60-61 -9.36652551791645 1101 62-63 -3.1064493595483107 1101 64-65 -4.206239782184024 1101 66-67 -8.943349155099689 1101 68-69 -4.349351719245881 1101 70-71 -4.363470434967066 1101 72-73 -4.582439445707685 1101 74-75 -6.123465953561739 1101 76-77 -7.223333188801417 1101 78-79 -10.359584529768084 1101 80-81 -7.26593125130729 1101 82-83 -3.772749020388172 1101 84-85 -3.176306405722407 1101 86-87 -2.69637284022836 1101 88-89 -3.017990151797214 1101 90-91 -8.750328457575575 1101 92-93 -15.447447271321447 1101 94-95 -10.058387559778527 1101 96-97 -8.364685836234695 1101 98-99 -8.394743344887182 1101 100-101 -8.284097074682462 1102 1 0.15115293434516275 1102 2 0.18994646435307772 1102 3 0.2699531205395367 1102 4 0.15350118595945617 1102 5 0.24431328606218017 1102 6 0.285231789445902 1102 7 0.3729304422454831 1102 8 0.3085421601614655 1102 9 0.44350607694269684 1102 10-11 0.3380598111671631 1102 12-13 0.18952134729492798 1102 14-15 0.3188441505338204 1102 16-17 0.3441649892655647 1102 18-19 0.32793261348245295 1102 20-21 0.40709456994842697 1102 22-23 0.6093296401759574 1102 24-25 0.42911883253612615 1102 26-27 0.462247470989233 1102 28-29 0.44044884201417034 1102 30-31 0.6355041729580009 1102 32-33 0.6320109190379597 1102 34-35 0.15683107148740305 1102 36-37 0.36701528921160786 1102 38-39 0.29425124159907057 1102 40-41 0.2776792968371069 1102 42-43 0.4188653178591579 1102 44-45 0.37901460598294534 1102 46-47 0.14087214975509 1102 48-49 0.08022134823264082 1102 50-51 0.12442907138466097 1102 52-53 0.15057452849960384 1102 54-55 -0.01729591110712647 1102 56-57 -2.715537453980261 1102 58-59 -9.80246934979602 1102 60-61 -10.120386541349571 1102 62-63 -4.235316860263627 1102 64-65 -4.927084690139026 1102 66-67 -5.984408365344386 1102 68-69 -3.8536965674972397 1102 70-71 -3.84234323015653 1102 72-73 -4.402752878577132 1102 74-75 -4.8466753511407 1102 76-77 -6.906188555032585 1102 78-79 -9.258942208670618 1102 80-81 -5.227590246618863 1102 82-83 -3.1393575604876176 1102 84-85 -2.873577385921074 1102 86-87 -2.823702690473599 1102 88-89 -3.0134436629347583 1102 90-91 -8.911938338190815 1102 92-93 -13.204143654632365 1102 94-95 -10.180650485617587 1102 96-97 -8.747234122965391 1102 98-99 -8.820949640129589 1102 100-101 -8.726498828392536 1103 1 0.14800535952576155 1103 2 0.21917961510325767 1103 3 0.16694446768568483 1103 4 0.20094502179338036 1103 5 0.28771250809916893 1103 6 0.22210961750020175 1103 7 0.05059179018547155 1103 8 0.11604394630101922 1103 9 0.18215019855895065 1103 10-11 0.30285896969697745 1103 12-13 0.234303835188868 1103 14-15 0.4567797701257845 1103 16-17 0.3432401658949189 1103 18-19 0.5198513242866127 1103 20-21 0.3463817018052211 1103 22-23 0.3700683081127707 1103 24-25 0.3982781561846096 1103 26-27 0.4192193690602082 1103 28-29 0.238779793828094 1103 30-31 0.2953755709098971 1103 32-33 0.5613849763531888 1103 34-35 0.3716163378206545 1103 36-37 0.2933489400808611 1103 38-39 0.08806722953271162 1103 40-41 0.22095348910509927 1103 42-43 0.20960914575469047 1103 44-45 0.12072691843829375 1103 46-47 0.11663026676708199 1103 48-49 0.017357174222951244 1103 50-51 -0.080478843198172 1103 52-53 -0.17046222628327712 1103 54-55 -0.43515929127701014 1103 56-57 -3.208539756115691 1103 58-59 -9.906448471809199 1103 60-61 -10.096539593663614 1103 62-63 -4.277352662438748 1103 64-65 -5.465933225504589 1103 66-67 -6.424848549515222 1103 68-69 -4.093289327678235 1103 70-71 -3.9884057847330165 1103 72-73 -4.283097405051688 1103 74-75 -5.129411320022179 1103 76-77 -6.656319538600101 1103 78-79 -8.957779233359037 1103 80-81 -5.089827287184079 1103 82-83 -3.330599522859611 1103 84-85 -2.9809898650827797 1103 86-87 -2.575981010708574 1103 88-89 -2.605159944589122 1103 90-91 -4.993610561736105 1103 92-93 -9.03428972115614 1103 94-95 -7.790849342488272 1103 96-97 -7.349107584900771 1103 98-99 -7.55978229869909 1103 100-101 -7.412832876181692 1104 1 0.23147172542315175 1104 2 0.25612507175199184 1104 3 0.1222455261621711 1104 4 0.1918612112780238 1104 5 0.19176132526615675 1104 6 0.13115872386841687 1104 7 0.2598288773507207 1104 8 0.16817535933821404 1104 9 0.2468213767647498 1104 10-11 0.20384948389675372 1104 12-13 0.34737428460758224 1104 14-15 0.42521201027848576 1104 16-17 0.3660321145327856 1104 18-19 0.3219393571398683 1104 20-21 0.4005837956220972 1104 22-23 0.37718699629601105 1104 24-25 0.5381937094265581 1104 26-27 0.5774370219030516 1104 28-29 0.6071194895883991 1104 30-31 0.30730222947892116 1104 32-33 0.48916998416162727 1104 34-35 0.3227095184557456 1104 36-37 0.48032187084609035 1104 38-39 0.2818089149974554 1104 40-41 0.2602429216922957 1104 42-43 0.10133220666046583 1104 44-45 0.0865751456347823 1104 46-47 0.008664218991036421 1104 48-49 -0.007583539525668925 1104 50-51 -0.143014270348381 1104 52-53 0.022677920630947312 1104 54-55 0.034360345486255994 1104 56-57 -2.790328754901047 1104 58-59 -10.174218441153869 1104 60-61 -10.225800973731864 1104 62-63 -4.003136397761789 1104 64-65 -5.229402772615469 1104 66-67 -6.232279575022769 1104 68-69 -4.190168846447396 1104 70-71 -3.8412226046763323 1104 72-73 -4.072449015902519 1104 74-75 -4.639381532295879 1104 76-77 -6.414327520553945 1104 78-79 -8.76940801466507 1104 80-81 -4.9060861801217825 1104 82-83 -2.8260720776632446 1104 84-85 -2.264824962543589 1104 86-87 -2.134217461631124 1104 88-89 -2.175930376655753 1104 90-91 -4.291312857993841 1104 92-93 -6.437757243858437 1104 94-95 -7.275696644240831 1104 96-97 -7.16904511743591 1104 98-99 -7.426965189554547 1104 100-101 -7.4938525997053205 1105 1 0.12015992447674151 1105 2 0.21485210284835432 1105 3 0.19195595665627252 1105 4 0.19865105733259725 1105 5 0.19309317599132925 1105 6 0.0775745378433399 1105 7 0.13061247033252954 1105 8 0.1772188850265124 1105 9 0.44612078679424627 1105 10-11 0.3787934059714857 1105 12-13 0.21348007599769403 1105 14-15 0.35356285836108015 1105 16-17 0.3399141089096247 1105 18-19 0.3525680828650408 1105 20-21 0.354684397883652 1105 22-23 0.2738903056617872 1105 24-25 0.425238940498339 1105 26-27 0.3517944303347136 1105 28-29 0.5885825664996602 1105 30-31 0.7024336285079329 1105 32-33 0.5564274855443685 1105 34-35 0.5786322691932071 1105 36-37 0.2269082231690973 1105 38-39 0.31653689865035517 1105 40-41 0.35382151912961035 1105 42-43 0.34762040494586444 1105 44-45 0.4149747738304512 1105 46-47 0.5891907753455072 1105 48-49 0.5075781454239348 1105 50-51 0.006563512806728511 1105 52-53 0.3564928135451524 1105 54-55 0.08437004389455893 1105 56-57 1.1722277071196032 1105 58-59 -8.591738759799394 1105 60-61 -10.04044086510969 1105 62-63 -3.7210413879289455 1105 64-65 -4.687028890333021 1105 66-67 -5.507651475373063 1105 68-69 -3.6834241315998035 1105 70-71 -3.8620194489487023 1105 72-73 -2.0954078370369835 1105 74-75 -3.933591390487866 1105 76-77 -5.62325428124716 1105 78-79 -8.484730443530605 1105 80-81 -4.575490966718391 1105 82-83 -2.368821413178239 1105 84-85 -1.932929586283759 1105 86-87 -1.8595402937968082 1105 88-89 -1.8317569115008858 1105 90-91 -4.273704005363555 1105 92-93 -6.250010462577709 1105 94-95 -7.194378371287291 1105 96-97 -7.4150236388223405 1105 98-99 -7.655420319532423 1105 100-101 -7.605024205838555 1106 1 0.18757934172908364 1106 2 0.27298306419373475 1106 3 0.33594990118438517 1106 4 0.31382398864116823 1106 5 0.21676991419413838 1106 6 0.30747460686944095 1106 7 0.2249755999342682 1106 8 0.31899693472530544 1106 9 0.2372017774234294 1106 10-11 0.33762351808660895 1106 12-13 0.3231001971071308 1106 14-15 0.4313427278389881 1106 16-17 0.36911228180955646 1106 18-19 0.5042437961347872 1106 20-21 0.3701316033722719 1106 22-23 0.4421074450526845 1106 24-25 0.6181773608463956 1106 26-27 0.5598741450268179 1106 28-29 0.7076336405876802 1106 30-31 0.5969504980695177 1106 32-33 0.3508349330426981 1106 34-35 0.2769986513886522 1106 36-37 0.573514701164413 1106 38-39 0.36612849419921645 1106 40-41 0.1494931378963429 1106 42-43 0.24681679990067806 1106 44-45 0.15868660030301385 1106 46-47 0.06718739724978917 1106 48-49 -0.2218506374157556 1106 50-51 -0.19823811558182314 1106 52-53 -0.2172611868313581 1106 54-55 0.20424341759770215 1106 56-57 1.3117728669254944 1106 58-59 -8.823191916174896 1106 60-61 -9.867962540352327 1106 62-63 -3.672409359816495 1106 64-65 -5.242126232828003 1106 66-67 -6.804793820796423 1106 68-69 -3.998789760783204 1106 70-71 -3.8446818694640967 1106 72-73 -4.1929013266203174 1106 74-75 -4.869991286948709 1106 76-77 -5.933246855711685 1106 78-79 -8.522449679850887 1106 80-81 -4.417553879829168 1106 82-83 -2.387491797841893 1106 84-85 -1.9877994563891797 1106 86-87 -1.7427933581375044 1106 88-89 -1.7980530857813406 1106 90-91 -3.8603500687839016 1106 92-93 -5.252106084649331 1106 94-95 -5.904856350914132 1106 96-97 -6.542894497256075 1106 98-99 -6.220659139395208 1106 100-101 -7.185433080922223 1107 1 0.12435867597195838 1107 2 -0.005170699185139682 1107 3 -0.0341813414896599 1107 4 0.2921590193541306 1107 5 -0.029812588063691692 1107 6 -0.09896646843656498 1107 7 -0.1700949534114926 1107 8 0.06008184854325549 1107 9 0.19259207866027594 1107 10-11 0.13740958420115135 1107 12-13 0.03547889851563468 1107 14-15 0.05394413826538624 1107 16-17 0.07801359679837816 1107 18-19 0.08505443902532761 1107 20-21 0.20440778305111706 1107 22-23 0.1368968098483876 1107 24-25 0.20454516059403005 1107 26-27 0.03798050988016399 1107 28-29 0.26316998052477913 1107 30-31 0.37089860308927314 1107 32-33 0.45470752068192866 1107 34-35 0.33109189837981035 1107 36-37 0.49995603267866784 1107 38-39 0.24998673417667305 1107 40-41 0.23277683088200263 1107 42-43 0.32027618769108557 1107 44-45 0.3192305304093992 1107 46-47 0.42812524753450276 1107 48-49 0.4906004428222559 1107 50-51 0.6059528108031387 1107 52-53 0.5976117018525642 1107 54-55 0.416982091591926 1107 56-57 1.743289683794245 1107 58-59 -8.27389616859126 1107 60-61 -9.861939725008018 1107 62-63 -3.404608971661002 1107 64-65 -4.180538254118904 1107 66-67 -6.135555463859905 1107 68-69 -3.282870901934732 1107 70-71 -3.088288512943917 1107 72-73 -1.7002378497463155 1107 74-75 -4.174389742012986 1107 76-77 -6.295919876342854 1107 78-79 -6.981332099481563 1107 80-81 -1.9595617838535588 1107 82-83 -0.8424369372512075 1107 84-85 -0.6199029901414157 1107 86-87 -0.6207459980432191 1107 88-89 -0.5202035696946652 1107 90-91 -0.3380823695980055 1107 92-93 -3.075545936560964 1107 94-95 -6.045955356185617 1107 96-97 -6.443234613702724 1107 98-99 -6.178881182271658 1107 100-101 -6.582712789869656 1108 1 -0.3093979756400813 1108 2 -0.1966074473852899 1108 3 -0.17076856145120445 1108 4 -0.037711749326284405 1108 5 -0.21287389468623985 1108 6 -0.24429133540432701 1108 7 -0.3384131575880289 1108 8 -0.4829848240856336 1108 9 -0.4796969702151017 1108 10-11 -0.5245141290752429 1108 12-13 -0.3989149663504392 1108 14-15 -0.29455337995667463 1108 16-17 -0.46220238495719457 1108 18-19 -0.514004274027478 1108 20-21 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88-89 1.077882554707248 2316 90-91 2.027467869636446 2316 92-93 3.6230186567404736 2316 94-95 10.073661868694765 2316 96-97 11.763942419790098 2316 98-99 11.438343698111117 2316 100-101 10.272789602474843 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 9.0 4 31.0 5 117.0 6 229.0 7 432.0 8 861.0 9 1475.0 10 2624.0 11 4014.0 12 5969.0 13 7866.0 14 9775.0 15 12452.0 16 16190.0 17 21410.0 18 21139.0 19 18609.0 20 18558.0 21 18496.0 22 18225.0 23 15884.0 24 15990.0 25 18412.0 26 25592.0 27 37175.0 28 51411.0 29 67383.0 30 81505.0 31 89127.0 32 89245.0 33 81961.0 34 73903.0 35 64825.0 36 49744.0 37 30022.0 38 11198.0 39 769.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.826811400209486 19.31121953007065 14.509422304288567 19.352546765431295 2 14.074970434796768 24.42472526242298 39.18605621561328 22.31424808716697 3 22.592203991121796 29.23101347301276 31.868015562301615 16.30876697356383 4 11.086245919123334 16.844861555495854 40.90524224735201 31.163650278028804 5 10.663886714928585 42.196977108198006 33.897679840866104 13.241456336007303 6 24.64198194237517 38.957717639971015 18.977195939069762 17.423104478584047 7 23.241457636225974 38.114980989831395 21.0876503105944 17.55591106334823 8 22.80612483742477 31.48453830669372 22.093032722654286 23.616304133227224 9 25.6464271466836 19.644180120329892 23.219984694168318 31.489408038818194 10-11 22.640520561105276 25.9157039461365 28.538862972099423 22.9049125206588 12-13 30.038966774947284 24.573187113792343 27.101295296713317 18.286550814547052 14-15 24.910202127264412 25.868096987299815 30.927969868418575 18.2937310170172 16-17 18.210937563604688 29.362823518875835 31.986033428587714 20.440205488931767 18-19 17.587240365142527 31.138119129275516 33.717167194162606 17.557473311419354 20-21 19.921801854610884 28.22704735852282 30.885367055011436 20.965783731854856 22-23 18.603489114644155 27.925485496793033 30.036102185212606 23.434923203350202 24-25 20.33453028933023 27.92175907244985 32.13152641656613 19.612184221653795 26-27 21.937690592121033 25.281731600498052 34.25633390442064 18.524243902960276 28-29 18.656113770989478 27.339150859627797 35.150067855309175 18.854667514073554 30-31 18.38238100496626 30.094943617526376 33.109353857208504 18.41332152029886 32-33 18.053388696373414 29.61708926781362 32.07214496160716 20.25737707420581 34-35 20.019723213499717 26.57443503119535 32.3260212589374 21.07982049636754 36-37 21.03433223464885 26.476513263418898 33.754317442902284 18.734837059029967 38-39 22.248301055683402 25.338642820833318 34.30344556400062 18.10961055948266 40-41 18.97088533956576 27.390928272406533 35.033564841936034 18.604621546091675 42-43 17.3014646362505 30.161185891280397 32.73447501366522 19.802874458803892 44-45 18.512120861717392 29.870261519645176 30.488517413503008 21.12910020513442 46-47 19.13998431148827 29.149101985513592 28.739316021637922 22.971597681360215 48-49 20.10097655493911 27.98875117918041 30.11652238048765 21.79374988539283 50-51 21.12064179294537 29.149369667642937 30.975015917483766 18.754972621927926 52-53 19.287588719119228 29.718365016823338 31.404161962395367 19.589884301662067 54-55 17.105305368408136 28.239658913323428 32.710540211758484 21.94449550650995 56-57 19.76657316220755 30.37301831779986 28.902293127780442 20.958115392212147 58-59 19.146016907340616 28.19127864219967 30.66816370310685 21.99454074735286 60-61 19.654126014929282 27.399202789418542 31.23833649816658 21.708334697485597 62-63 23.191064409022097 26.8730774612948 29.6986273487094 20.237230780973707 64-65 20.999281642135507 28.456447643939047 31.37715825780386 19.16711245612158 66-67 19.90573567586026 27.6654166593147 31.03862014134459 21.39022752348045 68-69 20.59112623907057 29.425622739608688 29.708704069398962 20.27454695192178 70-71 20.478189096957596 29.16803707109833 28.695745077542195 21.65802875440188 72-73 19.700450949797325 27.82350837269035 29.08495467494384 23.39108600256849 74-75 20.018505780328802 29.30261990311741 29.272590871590044 21.406283444963748 76-77 19.006106253036474 27.862728829599554 31.23033228404428 21.900832633319688 78-79 18.62656242935415 27.45570119733159 32.1672400540916 21.75049631922266 80-81 18.859693334677374 30.60808684947659 30.11685009769752 20.415369718148526 82-83 18.567333144045207 30.011275873193238 28.966476666168006 22.45491431659355 84-85 19.018053091221056 28.040935062503213 28.82551358370576 24.11549826256997 86-87 20.08312419984816 28.69255444085542 29.200987964902207 22.023333394394204 88-89 21.35958393354986 26.564739681152087 29.926783336377877 22.148893048920172 90-91 18.927352585586828 27.35207545822302 31.388263981349695 22.332307974840457 92-93 17.63043035105852 29.883171231522464 31.028621438924564 21.457776978494454 94-95 17.9672828131648 29.686517005113455 29.43305205772081 22.913148124000934 96-97 17.489324641019365 29.088272420863014 28.29535011994287 25.12705281817475 98-99 18.065992366234866 28.711031580534264 28.662242807011744 24.560733246219126 100-101 19.701156204787583 28.57630211758516 29.227412287912667 22.495129389714585 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3486.0 1 2578.5 2 1668.5 3 1700.0 4 1675.5 5 1888.0 6 2575.5 7 3652.0 8 4415.5 9 4503.0 10 4363.5 11 4173.0 12 4142.0 13 4148.5 14 4023.5 15 3875.0 16 3693.5 17 3517.5 18 3473.0 19 3562.5 20 3718.0 21 3918.0 22 4242.5 23 4716.5 24 5392.5 25 6350.0 26 7610.0 27 9182.0 28 10689.5 29 12055.5 30 13792.5 31 15768.0 32 18261.5 33 21090.0 34 23399.5 35 25404.0 36 26894.5 37 27438.0 38 27124.5 39 26213.0 40 25524.5 41 25138.0 42 25346.5 43 27218.5 44 31242.0 45 37526.0 46 46867.5 47 58981.5 48 71158.5 49 65836.5 50 46747.0 51 32241.5 52 21590.0 53 15174.0 54 11898.5 55 10431.5 56 9841.0 57 9657.5 58 9575.0 59 9172.5 60 8425.0 61 7156.0 62 5496.5 63 3724.0 64 2298.5 65 1502.5 66 970.5 67 679.5 68 542.0 69 465.0 70 392.0 71 331.5 72 282.5 73 212.5 74 138.5 75 88.5 76 54.0 77 28.0 78 21.0 79 14.0 80 4.0 81 3.0 82 1.5 83 1.0 84 1.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023304757019922004 2 0.005902514878408194 3 1.0176749790358954E-4 4 0.0 5 0.1343330972327382 6 0.0 7 0.0 8 0.0010176749790358954 9 0.0 10-11 0.0 12-13 0.0 14-15 8.650237321805111E-4 16-17 0.0 18-19 2.0353499580717907E-4 20-21 0.0 22-23 4.5795374056615293E-4 24-25 5.088374895179477E-5 26-27 3.561862426625634E-4 28-29 0.002696838694445123 30-31 0.01032940103721434 32-33 0.0027477224433969175 34-35 0.0034092111797702496 36-37 0.1021236841462521 38-39 0.1010551254182644 40-41 0.1118424801960449 42-43 0.11453931889049004 44-45 0.10995978148482852 46-47 0.10400638285746851 48-49 0.10359931286585415 50-51 0.10247987038891468 52-53 0.08258432454876292 54-55 0.07240757475840397 56-57 0.5592124009802245 58-59 1.6112847943075332 60-61 0.5316334090483518 62-63 0.004528653656709735 64-65 0.12486871992770437 66-67 1.9509847023097153 68-69 2.9352290582842815 70-71 2.8419591464556415 72-73 2.6895114345960645 74-75 2.0587564825896165 76-77 1.4449458189841162 78-79 0.9635346701511859 80-81 0.05281733141196298 82-83 3.0530249371076864E-4 84-85 2.5441874475897385E-4 86-87 6.106049874215373E-4 88-89 5.088374895179477E-5 90-91 0.2530957672862272 92-93 0.40478022291152743 94-95 2.0528030839622566 96-97 2.4899962549560772 98-99 1.4421980965407193 100-101 0.9614993201931141 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 982632.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.74695764244215 #Duplication Level Percentage of deduplicated Percentage of total 1 73.40642245443254 24.038370067985163 2 13.191029344242125 8.639321583922166 3 5.066498356877749 4.977372212645351 4 2.500772744337279 3.2757079652874674 5 1.4626097111711172 2.3948009129572565 6 0.9210615795388597 1.809717871874394 7 0.6250343002405966 1.4327580228536596 8 0.4602964724107682 1.2058647268000773 9 0.32079466516433464 0.945454438085209 >10 1.715694258896748 10.357498949677 >50 0.17252269762987701 3.91035460558685 >100 0.12830203367106735 8.654990621667984 >500 0.01027661920180582 2.2565732991980907 >1k 0.013702158935741095 9.83579603704157 >5k 0.003114127030850248 7.126235635753912 >10k+ 0.001868476218510149 9.13918304866385 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 23384 2.379731170977538 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 20475 2.0836895195759957 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 12878 1.3105618380024262 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11642 1.1847772105935894 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 10827 1.1018366998021638 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 10413 1.059704955670078 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 9368 0.9533579203608268 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 8567 0.8718421545400517 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8512 0.8662449421553541 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 8009 0.8150558907098487 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7353 0.7482964120850939 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6005 0.6111138249110551 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5960 0.6065342875053937 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 5741 0.5842472054645076 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 5248 0.534075828998038 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5117 0.5207442867726677 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 4623 0.47047114280829444 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 4581 0.46619690789634366 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4547 0.46273681296762165 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 4211 0.42854293367201557 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 3845 0.3912960294393018 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3778 0.3844776070797613 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 3395 0.3455006553826865 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 3037 0.30906789113320143 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2963 0.3015370962883358 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2722 0.27701112929357075 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2710 0.2757899193187277 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2685 0.27324573187113793 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2613 0.26591847202207947 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 2605 0.26510433203885075 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 2496 0.25401167476735953 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 2355 0.23966245756295335 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2331 0.23722003761326724 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2280 0.23202989522018413 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2167 0.22053016795707853 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 2157 0.21951249297804265 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2059 0.20953927818349086 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1993 0.20282262332185397 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 1873 0.19061052357342323 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 1739 0.17697367885434223 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1724 0.17544716638578836 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1617 0.16455804411010427 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1549 0.1576378542526602 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1530 0.155704271792492 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1524 0.15509366680507045 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1441 0.14664696447907252 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 1430 0.14552752200213304 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1399 0.14237272956712177 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 1397 0.1421691945713146 No Hit GTACATGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTT 1377 0.1401338446132428 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1366 0.1390144021363033 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 1292 0.13148360729143768 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1240 0.12619169740045103 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1185 0.12059448501575362 No Hit TACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGA 1184 0.12049271751785001 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1145 0.11652378509961003 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1118 0.1137760626562131 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1108 0.1127583876771772 No Hit CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT 1046 0.10644880280715466 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1013 0.10309047537633621 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.5441874475897385E-4 0.0 12-13 0.0 0.0 0.0 6.61488736373332E-4 0.0 14-15 0.0 0.0 0.0 9.159074811323059E-4 0.0 16-17 0.0 0.0 0.0 0.001984466209119996 0.0 18-19 0.0 0.0 0.0 0.002595071196541533 0.0 20-21 0.0 0.0 0.0 0.003765397422432813 0.0 22-23 0.0 0.0 0.0 0.008141399832287163 0.0 24-25 0.0 0.0 0.0 0.013178890978514846 0.0 26-27 0.0 0.0 0.0 0.017351358392562015 0.0 28-29 0.0 0.0 0.0 0.031547924350112756 0.0 30-31 0.0 0.0 0.0 0.06579268739467065 0.0 32-33 0.0 0.0 0.0 0.1311783047977269 0.0 34-35 0.0 0.0 0.0 0.24515790244974722 0.0 36-37 0.0 0.0 0.0 0.40259222170660025 0.0 38-39 0.0 0.0 0.0 0.5802273892973158 0.0 40-41 0.0 0.0 0.0 0.79042815621718 0.0 42-43 0.0 0.0 0.0 0.9881115208949027 0.0 44-45 0.0 0.0 0.0 1.1993808465427547 0.0 46-47 0.0 0.0 0.0 1.460821548657076 0.0 48-49 0.0 0.0 0.0 1.7307089530973956 0.0 50-51 0.0 0.0 0.0 1.9704222944093008 0.0 52-53 0.0 0.0 0.0 2.167902124091216 0.0 54-55 0.0 0.0 0.0 2.361616556350699 0.0 56-57 0.0 0.0 0.0 2.521035341816672 0.0 58-59 0.0 0.0 0.0 2.6528751353507722 0.0 60-61 0.0 0.0 0.0 2.7933142824577257 0.0 62-63 0.0 0.0 0.0 2.898948945281652 0.0 64-65 0.0 0.0 0.0 2.972425078768043 0.0 66-67 0.0 0.0 0.0 3.0360806487067387 0.0 68-69 0.0 0.0 0.0 3.1251272093723794 0.0 70-71 0.0 0.0 0.0 3.2718759413493554 0.0 72-73 0.0 0.0 0.0 3.494695878009265 0.0 74-75 0.0 0.0 0.0 3.755169788893502 0.0 76-77 0.0 0.0 0.0 4.09191843945648 0.0 78-79 0.0 0.0 0.0 4.640648788152635 0.0 80-81 0.0 0.0 0.0 5.304987014467267 0.0 82-83 0.0 0.0 0.0 5.9317730340554755 0.0 84-85 0.0 5.088374895179477E-5 0.0 6.556625471183516 0.0 86-87 0.0 1.0176749790358954E-4 0.0 7.0551844434132 0.0 88-89 0.0 1.0176749790358954E-4 0.0 7.411472453573667 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCACT 170 0.0 47.043484 1 GGGCGAG 55 5.2918083E-5 42.714573 7 CATTCCG 185 0.0 33.015533 9 CTTTGCG 430 0.0 28.437656 2 CGGCTTG 370 0.0 26.889387 74-75 TGGGCGA 70 0.008848225 26.84916 6 GGCAACG 65 8.278053E-6 26.216019 66-67 GCGACTC 65 8.289922E-6 26.21118 68-69 TGCGGAC 75 8.555289E-7 25.965654 64-65 GTAGCAA 870 0.0 25.741814 64-65 GTACATG 7600 0.0 25.193024 1 TACATGG 7420 0.0 25.163755 2 CGGGAGT 730 0.0 25.12642 4 GTCGGGA 755 0.0 24.917355 2 CTGCGCG 100 1.603621E-8 24.312037 62-63 ACGAGAG 80 1.6123522E-6 24.1442 88-89 GCTTACG 380 0.0 24.022337 60-61 ACATGGG 7300 0.0 23.966738 3 GTCACTA 375 0.0 23.829296 2 TGCGTGG 380 0.0 23.672247 62-63 >>END_MODULE