FastQCFastQC Report
Fri 27 May 2016
SRR939587_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939587_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3740857
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74730.19976705872477885No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT49650.13272359782798435No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49410.13208203360887627No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA148700.026.9327491
TACATGG106700.024.9938242
GTACATG107650.024.948911
TCAACGC165250.024.0915034
ATCAACG166200.023.86913
CAACGCA166900.023.7967855
TATCAAC168850.023.5225942
ACATGGG108400.023.376143
AACGCAG173000.023.1463036
GGTATCA105550.021.354261
ACGCAGA189100.020.7488217
CGCAGAG188750.020.4867638
AGAGTAC121650.020.00873210-11
CATGGGG63950.019.7371524
GAGTACT75300.019.691612-13
GCAGAGT199350.017.9698739
AGTACTT85900.017.31690812-13
GTACTTT85450.016.77053614-15
ATGGGGA38700.016.552825
GTGTAGC24550.016.2359221
Produced by FastQC (version 0.11.5)