##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939587_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3740857 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.102182467814192 31.0 30.0 33.0 26.0 34.0 2 30.24724655339672 31.0 30.0 34.0 26.0 34.0 3 30.296756598822142 31.0 30.0 34.0 26.0 34.0 4 33.87291013797106 35.0 35.0 37.0 28.0 37.0 5 33.85441036639465 35.0 35.0 37.0 28.0 37.0 6 33.94897318983324 36.0 35.0 37.0 29.0 37.0 7 33.75172186480264 35.0 33.0 37.0 28.0 37.0 8 33.746323101898845 35.0 33.0 37.0 28.0 37.0 9 35.02319014065493 37.0 34.0 39.0 28.0 39.0 10-11 34.893091342438375 37.0 34.0 39.0 27.0 39.0 12-13 34.74006464294145 37.0 34.0 39.0 27.0 39.0 14-15 35.42791878973187 38.0 34.0 40.0 27.0 41.0 16-17 35.14659060744637 38.0 33.0 40.0 26.5 41.0 18-19 35.07861153206338 38.0 33.5 40.0 26.0 41.0 20-21 35.19815833644537 38.0 34.0 40.0 26.0 41.0 22-23 35.04851321501998 38.0 34.0 40.0 25.0 41.0 24-25 34.925233843474906 38.0 34.0 40.0 25.0 41.0 26-27 35.056863306990884 38.0 34.0 40.0 25.0 41.0 28-29 34.94072213933866 38.0 34.0 40.0 25.0 41.0 30-31 34.735488686148656 38.0 33.0 40.0 24.0 41.0 32-33 34.525332430509906 38.0 33.0 40.0 24.0 41.0 34-35 34.29896812414909 38.0 33.0 40.0 24.0 41.0 36-37 34.025143169065274 38.0 33.0 40.0 23.0 41.0 38-39 33.86563747291062 38.0 33.0 40.0 22.0 41.0 40-41 33.60386256411299 38.0 32.0 40.0 20.0 41.0 42-43 33.30411079600209 37.5 31.0 40.0 18.5 41.0 44-45 33.06453334088953 37.0 31.0 40.0 17.5 41.0 46-47 32.6590091521809 37.0 31.0 40.0 15.0 41.0 48-49 32.326430815184864 37.0 30.0 39.5 13.5 41.0 50-51 31.752503370217042 36.0 30.0 38.5 12.0 40.0 52-53 31.836745831235998 36.5 30.0 39.0 9.0 40.0 54-55 31.874813445154413 36.5 30.0 39.0 9.0 40.0 56-57 31.712187073710652 36.0 30.0 39.0 8.0 40.0 58-59 31.297800610929528 36.0 29.5 39.0 7.5 40.0 60-61 30.861685036343275 35.0 28.5 39.0 4.0 40.0 62-63 30.435560086899873 35.0 28.0 38.5 2.0 40.0 64-65 29.810376873534594 34.5 27.5 38.0 2.0 40.0 66-67 29.254085494313202 34.0 26.5 38.0 2.0 40.0 68-69 28.711732097751934 34.0 26.0 37.0 2.0 39.0 70-71 28.13981408538204 33.5 26.0 36.5 2.0 39.0 72-73 27.376446226092042 32.5 24.5 36.0 2.0 39.0 74-75 26.892899675127918 32.0 23.0 35.5 2.0 38.0 76-77 26.478176658450188 32.0 22.5 35.0 2.0 37.5 78-79 25.86911154315709 31.5 21.0 35.0 2.0 37.0 80-81 25.166734788311878 31.0 19.0 34.5 2.0 36.0 82-83 24.46209625227588 31.0 16.5 34.0 2.0 36.0 84-85 23.76048924083439 30.0 9.0 34.0 2.0 35.0 86-87 23.05797334674915 29.5 4.5 34.0 2.0 35.0 88-89 22.228232728489758 29.0 2.0 34.0 2.0 35.0 90-91 20.92979456311749 28.0 2.0 33.5 2.0 35.0 92-93 19.215405988520814 25.0 2.0 32.0 2.0 34.0 94-95 18.95050733027218 25.0 2.0 32.0 2.0 34.0 96-97 18.29863691662098 25.0 2.0 32.0 2.0 34.0 98-99 17.27955719772234 22.0 2.0 32.0 2.0 34.0 100-101 15.641441252632752 9.5 2.0 30.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.0299514164092543 1101 2 1.002811235688437 1101 3 1.1782775901436047 1101 4 1.0659231077228029 1101 5 0.9702120559722829 1101 6 1.031416595980076 1101 7 0.9426846909014728 1101 8 0.9363599803744052 1101 9 1.1709486178488575 1101 10-11 1.0765666543424999 1101 12-13 1.004289919390331 1101 14-15 1.3259321879356847 1101 16-17 1.2306188193835794 1101 18-19 1.0585005023360665 1101 20-21 1.28204650662871 1101 22-23 1.4947834612359614 1101 24-25 1.2117521271065286 1101 26-27 1.1816014526890086 1101 28-29 1.327744509298654 1101 30-31 1.6257428725350564 1101 32-33 1.4392905581930862 1101 34-35 1.4362990433224354 1101 36-37 1.402741617523347 1101 38-39 1.3726864148711755 1101 40-41 1.3286429380864249 1101 42-43 1.3104630786669205 1101 44-45 1.3758791753129884 1101 46-47 1.6567510413774968 1101 48-49 1.4593141619393961 1101 50-51 1.3903976556631115 1101 52-53 1.7221990568021717 1101 54-55 1.4435173486877062 1101 56-57 1.3220437105638183 1101 58-59 1.3383346529398352 1101 60-61 1.0602225751268932 1101 62-63 1.14661237098354 1101 64-65 1.1446324974295017 1101 66-67 0.810904046327547 1101 68-69 1.0285801489454478 1101 70-71 0.8494817352975623 1101 72-73 1.0283676970037874 1101 74-75 0.7515420990469508 1101 76-77 0.49495032161092567 1101 78-79 0.6351056396556203 1101 80-81 0.5488776428295559 1101 82-83 0.40570848752795285 1101 84-85 0.07218651932966935 1101 86-87 -0.3520651842363094 1101 88-89 -0.8001890201051474 1101 90-91 -4.280688914074279 1101 92-93 -9.544068977737789 1101 94-95 -7.8885552837078485 1101 96-97 -4.387826811245803 1101 98-99 -3.245893931383849 1101 100-101 -2.505939552291096 1102 1 1.0948749647236582 1102 2 1.0212320704380033 1102 3 0.9906611010819049 1102 4 1.079034780346987 1102 5 0.9006174285691984 1102 6 0.9867820650426964 1102 7 1.050780470589146 1102 8 0.9664185159333769 1102 9 1.2212869886143238 1102 10-11 1.1136971731382488 1102 12-13 1.0420061517247703 1102 14-15 1.1948891525030945 1102 16-17 1.4281690162866738 1102 18-19 1.2410976901640467 1102 20-21 1.257196907469357 1102 22-23 1.2034405049879169 1102 24-25 1.1880899849154716 1102 26-27 1.1319416287625756 1102 28-29 1.125229326034976 1102 30-31 1.2885505374997592 1102 32-33 1.1953128170194915 1102 34-35 1.1284709005611617 1102 36-37 1.2505824690311584 1102 38-39 1.1993218995092434 1102 40-41 1.1188683152401921 1102 42-43 1.0948760640613457 1102 44-45 0.9153022019556047 1102 46-47 0.9737514721613678 1102 48-49 1.0386978390905526 1102 50-51 1.0882574784690071 1102 52-53 1.1194736796484115 1102 54-55 1.1591739299072827 1102 56-57 1.2142874377406798 1102 58-59 1.0049873129056976 1102 60-61 1.1301522739392489 1102 62-63 0.9766649459131678 1102 64-65 0.836615186392347 1102 66-67 0.9861906701150325 1102 68-69 0.9254071798859407 1102 70-71 0.7883338488153377 1102 72-73 0.87486725127944 1102 74-75 0.7635658400935554 1102 76-77 0.4649337283393713 1102 78-79 0.46985045935514336 1102 80-81 0.32594317987136634 1102 82-83 0.27349293560791565 1102 84-85 0.07013018002138693 1102 86-87 -0.5279732386495404 1102 88-89 -1.213873660170286 1102 90-91 -5.257634429253187 1102 92-93 -10.488234377576182 1102 94-95 -9.088339004684409 1102 96-97 -5.645680679647558 1102 98-99 -4.18337673426786 1102 100-101 -3.416314642400872 1103 1 0.9107057465674799 1103 2 0.922221975831949 1103 3 0.813231373396313 1103 4 0.8930398699951354 1103 5 0.8829289310328576 1103 6 0.7727364534674237 1103 7 0.9645241967479592 1103 8 0.8518804070899506 1103 9 0.8182180554643281 1103 10-11 0.8201422768073314 1103 12-13 0.8405215288845156 1103 14-15 0.8705995654033032 1103 16-17 1.0241318796166183 1103 18-19 0.990730625610091 1103 20-21 1.0583505473264694 1103 22-23 1.0550583532206517 1103 24-25 0.8726369274213894 1103 26-27 0.8086953048482854 1103 28-29 0.9682650094440461 1103 30-31 1.0196896094594052 1103 32-33 1.0878435495783023 1103 34-35 0.9556992095676193 1103 36-37 0.9872872776723796 1103 38-39 0.9448172903605894 1103 40-41 0.8765977699173888 1103 42-43 0.8300482881329074 1103 44-45 0.8024742919342742 1103 46-47 0.9781872704903805 1103 48-49 0.8383685910810712 1103 50-51 0.7523003405521287 1103 52-53 0.8580714474724651 1103 54-55 0.8153007541291544 1103 56-57 0.7183894006722298 1103 58-59 0.6309761008589732 1103 60-61 0.515799719058208 1103 62-63 0.6889835970564242 1103 64-65 0.544002763039952 1103 66-67 0.537837527695455 1103 68-69 0.5358974334508702 1103 70-71 0.6572328433539916 1103 72-73 0.740237044741118 1103 74-75 0.43322957573297316 1103 76-77 0.216702592796171 1103 78-79 0.20457908889062537 1103 80-81 -0.12002569705371613 1103 82-83 0.036838044217265775 1103 84-85 -0.06697846579344713 1103 86-87 -0.3825510548646669 1103 88-89 -0.75547024174287 1103 90-91 -2.2923605886566847 1103 92-93 -9.074381553921258 1103 94-95 -5.604812064954453 1103 96-97 -3.0234157626969242 1103 98-99 -2.159688756635493 1103 100-101 -1.7839978320940677 1104 1 0.8083410068782229 1104 2 0.8967364840571719 1104 3 0.818516442461835 1104 4 1.0068270700245208 1104 5 0.8997707342798833 1104 6 0.8091879378584039 1104 7 0.882626083992136 1104 8 0.826997690856814 1104 9 0.9348115787782589 1104 10-11 0.8213624586723824 1104 12-13 0.8124911994639135 1104 14-15 0.9363433949819253 1104 16-17 1.06350499178658 1104 18-19 0.9677708121240656 1104 20-21 1.0917447366893214 1104 22-23 1.0675685900176362 1104 24-25 0.9032809414998795 1104 26-27 0.9198942499119198 1104 28-29 0.8835093656674786 1104 30-31 0.9304709122070207 1104 32-33 0.9965107993650122 1104 34-35 0.8000638567852647 1104 36-37 0.8514922150228728 1104 38-39 0.7580185307468668 1104 40-41 0.6953500302464946 1104 42-43 0.8072229384983913 1104 44-45 0.7777391502532041 1104 46-47 0.4821238375009429 1104 48-49 0.40929998284843094 1104 50-51 0.4269734755866388 1104 52-53 0.5546092408085634 1104 54-55 0.7588654269348396 1104 56-57 0.7691581817507682 1104 58-59 0.572425612311207 1104 60-61 0.6889299716513726 1104 62-63 0.5603818437694095 1104 64-65 0.6302559718364122 1104 66-67 0.5839050459031796 1104 68-69 0.4444375347769487 1104 70-71 0.5251447837820074 1104 72-73 0.5220501912902513 1104 74-75 0.2409280270868912 1104 76-77 0.15572592794304896 1104 78-79 -0.005476058389408678 1104 80-81 -0.012606180604223027 1104 82-83 -0.07270369562970203 1104 84-85 -0.008986195406464503 1104 86-87 -0.1304930548988672 1104 88-89 -0.22619126060263994 1104 90-91 -0.6864062721643727 1104 92-93 -4.294219785610771 1104 94-95 -2.0245959854086024 1104 96-97 -1.0560616920777512 1104 98-99 -0.7797737798452324 1104 100-101 -0.7047694253228673 1105 1 0.9191527316983539 1105 2 0.8544156871408823 1105 3 0.8591214786637238 1105 4 0.8333561307134971 1105 5 0.6624982358130893 1105 6 0.7342256095434152 1105 7 0.8510863152445793 1105 8 0.7983106945956919 1105 9 1.10481977972686 1105 10-11 0.8983990221562479 1105 12-13 0.9061013207742192 1105 14-15 1.1104537286324145 1105 16-17 1.1419897283319216 1105 18-19 1.0034841873444478 1105 20-21 0.9760829734658572 1105 22-23 0.8950034620639755 1105 24-25 0.9560845147982135 1105 26-27 0.9585602592887099 1105 28-29 0.9361557161803162 1105 30-31 1.0396707701699697 1105 32-33 1.0379243541477976 1105 34-35 0.8607928892489198 1105 36-37 1.074689030635497 1105 38-39 0.9910230890695289 1105 40-41 0.9176238001529811 1105 42-43 1.0735446146916416 1105 44-45 0.9987885985264171 1105 46-47 1.1628076444320143 1105 48-49 0.8577807912629609 1105 50-51 0.9817957439261029 1105 52-53 0.889020154508696 1105 54-55 0.9575864756677248 1105 56-57 0.8091959802545574 1105 58-59 0.7816847824480462 1105 60-61 0.7682568825341782 1105 62-63 0.8056186274627244 1105 64-65 0.8904878095968023 1105 66-67 0.8062733771044392 1105 68-69 0.7540829169144914 1105 70-71 0.797559387583842 1105 72-73 1.1044858860328617 1105 74-75 0.9129345995521234 1105 76-77 0.768080842366377 1105 78-79 0.5815572078058402 1105 80-81 0.5759669583950249 1105 82-83 0.6541569799437674 1105 84-85 0.4758224415386465 1105 86-87 0.11531984380803806 1105 88-89 -0.224943883963185 1105 90-91 -1.397397721976322 1105 92-93 -7.530969140298222 1105 94-95 -3.2990616393025576 1105 96-97 -1.4755268484518282 1105 98-99 -0.8398795792361113 1105 100-101 -0.6719839708530895 1106 1 0.8274277991818089 1106 2 0.7975451278447139 1106 3 0.8845829137113235 1106 4 0.886045639625543 1106 5 0.9036126289036801 1106 6 0.8116045141433617 1106 7 0.819726648795438 1106 8 0.7324853185764368 1106 9 0.9110895356292943 1106 10-11 0.883881758273688 1106 12-13 1.0290830381854192 1106 14-15 0.9988294194085725 1106 16-17 1.0591170420393183 1106 18-19 1.063368639616428 1106 20-21 1.0270442588958417 1106 22-23 1.0991787890298 1106 24-25 0.9926671115006513 1106 26-27 1.07690367496253 1106 28-29 0.9988851583772131 1106 30-31 1.0234339923549385 1106 32-33 1.0892275671628582 1106 34-35 1.0389636007755811 1106 36-37 1.081630852710596 1106 38-39 1.1005219571391365 1106 40-41 1.0544414924831642 1106 42-43 1.0037041363134094 1106 44-45 0.9611640441909657 1106 46-47 1.227491058525679 1106 48-49 1.174001829677671 1106 50-51 1.0457545545997782 1106 52-53 0.9825283673028942 1106 54-55 0.9875546360428871 1106 56-57 0.9151380082483058 1106 58-59 1.062020065891005 1106 60-61 1.1688868318134986 1106 62-63 1.0597729270874048 1106 64-65 1.128539162967492 1106 66-67 0.9052900689562193 1106 68-69 0.8404149274331374 1106 70-71 0.7561184952531583 1106 72-73 0.8174412943832046 1106 74-75 0.9176761716631496 1106 76-77 0.8131872108512432 1106 78-79 0.8477702676510219 1106 80-81 0.7603695859417137 1106 82-83 0.8263296890678262 1106 84-85 0.7295093027476192 1106 86-87 0.7816269045776743 1106 88-89 0.48451969487783586 1106 90-91 0.024365871608718948 1106 92-93 -3.2972336798025594 1106 94-95 -1.181635681360973 1106 96-97 -0.2194843651352798 1106 98-99 0.0489109038390545 1106 100-101 0.26796345146239453 1107 1 0.6351427193142669 1107 2 0.6177356728187071 1107 3 0.6421628388763239 1107 4 0.6823763427211276 1107 5 0.5241628275160792 1107 6 0.5215295254761756 1107 7 0.5049455834339085 1107 8 0.5103949326460366 1107 9 0.5246956760947441 1107 10-11 0.40393047017430206 1107 12-13 0.44359392153040034 1107 14-15 0.5197330866309926 1107 16-17 0.6474292976154459 1107 18-19 0.4802617961322184 1107 20-21 0.5317975876704395 1107 22-23 0.6058545475601491 1107 24-25 0.7156373081154044 1107 26-27 0.4433377563897736 1107 28-29 0.5833688045474901 1107 30-31 0.5472674038014631 1107 32-33 0.49720798252016607 1107 34-35 0.4696539805593716 1107 36-37 0.469642270042506 1107 38-39 0.5847955044257915 1107 40-41 0.5053891463981728 1107 42-43 0.4822454684379025 1107 44-45 0.3203196917749622 1107 46-47 0.37748564182380306 1107 48-49 0.4612213328777486 1107 50-51 0.3738964721890774 1107 52-53 0.3942426574076201 1107 54-55 0.5058340456607908 1107 56-57 0.35710799741490007 1107 58-59 0.30387676531399066 1107 60-61 0.1777525513896876 1107 62-63 0.20147307998437824 1107 64-65 0.2715435116955298 1107 66-67 0.19646606950941603 1107 68-69 0.11970568523357272 1107 70-71 0.19417459077151378 1107 72-73 0.15025847877388543 1107 74-75 0.06011437900374261 1107 76-77 -0.16563582818742617 1107 78-79 -0.15203407800370883 1107 80-81 0.012688395342959069 1107 82-83 -0.017586547976268463 1107 84-85 -0.25109172066328966 1107 86-87 -0.2945050708666095 1107 88-89 -0.5315782052858182 1107 90-91 -1.0348153612479933 1107 92-93 -4.326753821481969 1107 94-95 -1.848315473605485 1107 96-97 -1.0000383669638957 1107 98-99 -0.7308437310906051 1107 100-101 -0.632645024715643 1108 1 0.5204815033559704 1108 2 0.4352843834797717 1108 3 0.10866902190414152 1108 4 -0.28314616375504187 1108 5 -0.7779080168232895 1108 6 -0.12741188790567293 1108 7 0.2085081927640502 1108 8 0.3466948660522249 1108 9 0.3578747497079817 1108 10-11 0.3537560198918328 1108 12-13 0.2399012790308106 1108 14-15 0.3015975857595308 1108 16-17 0.4397318820286742 1108 18-19 0.20874508236132527 1108 20-21 0.25153497228631494 1108 22-23 0.1781176854985418 1108 24-25 0.18499080652065913 1108 26-27 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76-77 0.7097965154481081 2311 78-79 0.7181401135013772 2311 80-81 0.6203057946589858 2311 82-83 0.8381941466783438 2311 84-85 0.6445986311427667 2311 86-87 0.8337975136938489 2311 88-89 1.0757703408789325 2311 90-91 2.0014616112428634 2311 92-93 2.988275402382108 2311 94-95 2.1581211344428333 2311 96-97 1.696323062014276 2311 98-99 1.5530184256849502 2311 100-101 1.3840081950279828 2312 1 0.20284084298528882 2312 2 -0.050094030860279304 2312 3 -0.04792642337607944 2312 4 -0.10543923891828655 2312 5 0.007024249360426893 2312 6 0.08932695481358621 2312 7 0.024858787702115137 2312 8 -0.7029524582426703 2312 9 -0.18252497432512627 2312 10-11 -0.08785601619455008 2312 12-13 0.03034170931935165 2312 14-15 -0.32481034105884277 2312 16-17 -0.08161877051855981 2312 18-19 -0.04541964870073656 2312 20-21 0.11707948840668791 2312 22-23 -0.009881338057780908 2312 24-25 0.13133701809771736 2312 26-27 0.18231655237305944 2312 28-29 0.0665090883102053 2312 30-31 0.1528827523304983 2312 32-33 0.088324961693381 2312 34-35 0.013035112805177107 2312 36-37 0.21106159950356584 2312 38-39 0.14951052456903824 2312 40-41 0.2747833979542591 2312 42-43 0.2532460213899199 2312 44-45 0.04778344762488729 2312 46-47 0.4302896888521275 2312 48-49 0.1999231047423251 2312 50-51 0.12970960987735225 2312 52-53 0.07901987256076382 2312 54-55 0.2564288640329835 2312 56-57 0.15962162373218547 2312 58-59 0.24623756407976316 2312 60-61 0.3330061596836451 2312 62-63 0.263602315991708 2312 64-65 0.4562857764128374 2312 66-67 0.27916718363516324 2312 68-69 -0.011801487827508339 2312 70-71 0.13766811897609443 2312 72-73 0.39183028112336515 2312 74-75 0.6510412265163446 2312 76-77 0.7104726471320539 2312 78-79 0.6987737316776261 2312 80-81 0.5580970397722496 2312 82-83 0.7220804556203397 2312 84-85 0.6941857951474333 2312 86-87 0.661082114188531 2312 88-89 1.0402601908631866 2312 90-91 1.4014380745507289 2312 92-93 2.553301441476531 2312 94-95 1.8283881586282327 2312 96-97 1.4993830163188946 2312 98-99 1.2301269173141343 2312 100-101 1.3297612998945905 2313 1 -0.2024864656630747 2313 2 -0.8266478745397485 2313 3 -0.4327659959373449 2313 4 -0.1971270733707584 2313 5 -0.9198673950798266 2313 6 -0.5355761900618674 2313 7 -1.3591632100901023 2313 8 -1.0815037561547598 2313 9 -1.2763388139682021 2313 10-11 -2.1349662128029223 2313 12-13 -0.6906018018777687 2313 14-15 -0.16874067879356147 2313 16-17 -1.509284964588545 2313 18-19 -1.1223959934208736 2313 20-21 -1.5231464333947784 2313 22-23 -1.2435131862176902 2313 24-25 -0.38702393582109096 2313 26-27 -1.0518831693523865 2313 28-29 -2.4109066955641296 2313 30-31 -0.6373222007856967 2313 32-33 -0.30253473109352313 2313 34-35 -0.02871646295412944 2313 36-37 -0.04895942162769984 2313 38-39 -0.47857920003704635 2313 40-41 -0.27923043962982064 2313 42-43 -0.14891704563626007 2313 44-45 -0.7821789680220448 2313 46-47 -0.8017561536082667 2313 48-49 -0.3769604882666613 2313 50-51 -0.4030234887698718 2313 52-53 -0.21968453657393283 2313 54-55 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2315 32-33 -1.7883596030741629 2315 34-35 -2.52145563367592 2315 36-37 -3.1209217989942886 2315 38-39 -2.2863978798233333 2315 40-41 -3.526091274796265 2315 42-43 -7.828915688955284 2315 44-45 -4.3193852475850605 2315 46-47 -2.766103393120545 2315 48-49 -5.649835780639368 2315 50-51 -6.6910443736998495 2315 52-53 -6.748494413052303 2315 54-55 -4.523193634434829 2315 56-57 -3.857755220053644 2315 58-59 -4.077527744464682 2315 60-61 -3.179734084040703 2315 62-63 -3.4507846159935376 2315 64-65 -2.1426948151495075 2315 66-67 -2.7142428552824818 2315 68-69 -2.1093420045697293 2315 70-71 -1.8270079301214643 2315 72-73 -2.340950226439535 2315 74-75 -3.0015046572943938 2315 76-77 -2.9621356812514534 2315 78-79 -2.0261746346433966 2315 80-81 -1.7234337497241192 2315 82-83 -2.0952067795737754 2315 84-85 -1.90797380537105 2315 86-87 -2.379420260744208 2315 88-89 -1.7791291953701176 2315 90-91 -0.9082771067152677 2315 92-93 -0.09579107718027657 2315 94-95 -0.23278087431143746 2315 96-97 -0.3395609406217446 2315 98-99 -0.5549332068428647 2315 100-101 -0.4749389098678467 2316 1 -4.441981689831845 2316 2 -3.0449988994511337 2316 3 -4.689989223542312 2316 4 -4.542625011546562 2316 5 -4.0368789975125345 2316 6 -4.623315257972049 2316 7 -3.1421112870718915 2316 8 -1.6005267557326022 2316 9 -1.9751031933101615 2316 10-11 -3.138408306441427 2316 12-13 -4.155924308660154 2316 14-15 -3.102357203706525 2316 16-17 -3.04727053550382 2316 18-19 -3.160701918230881 2316 20-21 -5.733040031264846 2316 22-23 -6.639515342263621 2316 24-25 -4.364485886558391 2316 26-27 -3.944268684682104 2316 28-29 -4.443901778137111 2316 30-31 -6.4781957994348005 2316 32-33 -4.582668473317604 2316 34-35 -6.144070605078404 2316 36-37 -9.709437352160492 2316 38-39 -6.414739501209709 2316 40-41 -5.610348667376911 2316 42-43 -4.450882016206442 2316 44-45 -3.422527241027151 2316 46-47 -5.880054309073074 2316 48-49 -3.2277835861719915 2316 50-51 -4.366474509786055 2316 52-53 -5.383802479023927 2316 54-55 -7.073832891859411 2316 56-57 -5.236020360950548 2316 58-59 -6.166376526430216 2316 60-61 -6.640554526624872 2316 62-63 -6.806623693363637 2316 64-65 -5.466059871352535 2316 66-67 -6.863991760342142 2316 68-69 -5.6384376574485735 2316 70-71 -6.4421978940039 2316 72-73 -8.494481982376108 2316 74-75 -8.324380217031308 2316 76-77 -7.521848596747446 2316 78-79 -6.440845555203957 2316 80-81 -6.420356610030428 2316 82-83 -5.544671202812747 2316 84-85 -7.0468774143541495 2316 86-87 -5.616083535389166 2316 88-89 -5.60853695691312 2316 90-91 -6.079560016259222 2316 92-93 -4.884499271579388 2316 94-95 -4.916719971521658 2316 96-97 -5.59237653197486 2316 98-99 -6.47637089071071 2316 100-101 -5.471653875510292 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 21502.0 3 8675.0 4 9198.0 5 10473.0 6 11448.0 7 13507.0 8 16180.0 9 19548.0 10 23248.0 11 26907.0 12 31218.0 13 34743.0 14 37609.0 15 41183.0 16 45305.0 17 49757.0 18 49433.0 19 49599.0 20 52798.0 21 58198.0 22 64005.0 23 71293.0 24 79768.0 25 89634.0 26 100807.0 27 113604.0 28 128407.0 29 146467.0 30 166059.0 31 189505.0 32 218141.0 33 252757.0 34 296268.0 35 355273.0 36 405827.0 37 347742.0 38 103163.0 39 1608.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.50505445887472 19.25419340960528 13.435097633743231 23.805654497776775 2 15.08683074793971 20.378528063559664 40.85309025736558 23.681550931135046 3 19.076895002679713 25.116105211643674 29.760955844834154 26.046043940842466 4 12.328391045388758 16.728419622910504 38.64545222724053 32.297737104460204 5 13.270948817065742 37.882445866185186 35.2886623950228 13.557942921726276 6 31.36736215502138 37.16390994098636 18.05762999976725 13.411097904225016 7 27.621302788402236 32.550820145848306 22.21335698585531 17.614520079894152 8 25.412224518118244 35.67405922591234 20.384488708891592 18.529227547077834 9 26.06439161264225 16.637309059621984 20.551198881913933 36.74710044582184 10-11 24.397470163303346 26.48605565110974 28.81113273696283 20.305341448624077 12-13 25.602228275723032 23.926045025970552 28.41957208619385 22.052154612112567 14-15 22.606420160714727 25.428540276009787 26.442649806314204 25.52238975696128 16-17 22.219720539271872 28.329900542718043 27.008731007276555 22.44164791073353 18-19 22.093907317071213 27.71844099731456 28.539658768657638 21.64799291695659 20-21 22.97893691108145 26.940189122923208 28.376051565599298 21.70482240039604 22-23 22.477078153524353 26.860278732543424 28.250850457674215 22.411792656258008 24-25 22.444214359478966 26.94433990092665 28.495352289645805 22.11609344994858 26-27 22.3422972445727 27.087283194055082 28.483407406118516 22.087012155253703 28-29 22.171955512712852 27.349263002294926 28.40399836279245 22.07478312219977 30-31 22.507339562268424 27.072645002365096 28.253988607382475 22.166026827984002 32-33 22.399845277483323 27.07433908322894 28.266438598181875 22.259377041105864 34-35 22.631339380152617 27.14422361284225 28.20829017666008 22.016146830345058 36-37 22.62249961955497 26.83671968448693 28.38062384419327 22.16015685176483 38-39 22.307269603872783 27.366367221606296 28.260205641187337 22.066157533333584 40-41 22.596630923903042 27.143150604727413 28.261576807909133 21.998641663460415 42-43 22.344744115884495 27.088785057985337 28.169374094501826 22.39709673162834 44-45 22.42892920646713 27.155045983520655 28.10562721393711 22.310397596075102 46-47 22.679197196985786 27.201719668866573 27.766078029934672 22.35300510421297 48-49 22.438458317537627 27.214397546364417 27.997570138295437 22.349573997802523 50-51 22.18573686848565 27.35847288445638 28.016949624507877 22.438840622550092 52-53 22.522097584572236 27.542455652535292 27.601486083398523 22.33396067949395 54-55 22.46726833864017 27.31078975705478 27.70665030457351 22.51529159973154 56-57 22.434503076759995 27.452172898171934 27.619033724972887 22.494290300095187 58-59 22.57737405846138 27.306138934845166 27.728953043584397 22.387533963109057 60-61 22.44026205284363 27.427062788244612 27.83141823776234 22.301256921149417 62-63 22.269798769804925 27.324534474849305 27.974653121067316 22.43101363427845 64-65 22.23412918337752 27.486932151016248 27.79742151329344 22.481517152312794 66-67 22.311077982940482 27.512398530108424 27.440068195261127 22.736455291689975 68-69 22.306613430531165 27.32681383816577 27.750881221121364 22.615691510181698 70-71 22.475279577284685 27.128715892360855 27.63085292902565 22.765151601328807 72-73 22.405530363533508 27.126528979630738 27.698576928153518 22.769363728682237 74-75 22.189877320696105 27.598298012263513 27.46140155993675 22.750423107103636 76-77 22.27181243278677 27.293376899643956 27.520629908440053 22.914180759129216 78-79 22.328929676039117 27.38465386150444 27.47806639667137 22.80835006578508 80-81 22.4108167267651 27.327574006360777 27.35329509045962 22.9083141764145 82-83 22.386320470569903 27.308339585095442 27.55868232180202 22.74665762253264 84-85 22.360055135545117 27.336457074043846 27.374674997058356 22.92881279335268 86-87 22.14121136377753 27.270744848325183 27.55678836634989 23.031255421547396 88-89 22.340664260871336 27.23174739363246 27.356736816059485 23.070851529436716 90-91 22.082595624310567 27.13601020246385 27.708076159848787 23.073318013376795 92-93 21.88172244595938 27.131793571932246 28.057714644881486 22.928769337226885 94-95 22.258231621471335 27.15810146545684 27.38186109042114 23.20180582265068 96-97 22.202597301036217 27.060863337925923 27.460537727899233 23.276001633138627 98-99 22.170655657148476 27.10957691204819 27.266892733940317 23.45287469686302 100-101 22.256340084830477 27.04513518643109 27.321764453520913 23.376760275217524 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3012.0 1 1739.5 2 454.5 3 434.0 4 403.0 5 426.5 6 578.0 7 926.5 8 1427.0 9 1742.5 10 1692.5 11 1567.5 12 1663.5 13 1892.0 14 2055.5 15 2250.0 16 2514.0 17 2782.0 18 3113.5 19 3569.5 20 4229.5 21 5166.5 22 6480.0 23 8246.5 24 10361.5 25 13222.5 26 18052.5 27 24063.5 28 29524.5 29 35430.0 30 42191.5 31 49810.0 32 58236.0 33 67081.0 34 77102.5 35 87164.0 36 98205.0 37 107217.0 38 112802.5 39 117325.5 40 121320.5 41 125201.5 42 127165.5 43 130600.0 44 135468.5 45 139036.0 46 141538.0 47 145131.5 48 150653.0 49 150084.0 50 142859.5 51 136365.0 52 131029.5 53 125684.0 54 119425.0 55 111340.5 56 101655.0 57 91793.5 58 82828.5 59 73102.5 60 61786.5 61 49972.0 62 38567.0 63 28654.5 64 21100.5 65 15642.0 66 11326.5 67 7946.5 68 5696.5 69 4207.5 70 3073.5 71 2013.0 72 1249.5 73 872.5 74 600.0 75 386.0 76 248.5 77 165.0 78 112.0 79 71.0 80 48.0 81 40.0 82 33.0 83 20.5 84 15.0 85 13.0 86 11.5 87 7.0 88 4.0 89 4.5 90 4.0 91 6.0 92 7.0 93 6.5 94 4.5 95 1.5 96 2.0 97 3.0 98 2.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08730619748362474 2 0.09741083393457703 3 0.09393569441440824 4 0.09767815235920539 5 0.09350798493500286 6 0.0777361978819292 7 0.0912090464831989 8 0.08741312485347609 9 0.08129153292948649 10-11 0.08830864157598112 12-13 0.08710570866515346 14-15 0.09328076427406876 16-17 0.09016650462714827 18-19 0.09304017769190322 20-21 0.10343886440994671 22-23 0.11207324952544297 24-25 0.13470175417023425 26-27 0.13979417015940465 28-29 0.1397540723957104 30-31 0.1411441282037779 32-33 0.13732147473159226 34-35 0.1324161816396617 36-37 0.13634576248169872 38-39 0.12924845830781556 40-41 0.12482433838021609 42-43 0.12440999482204211 44-45 0.11497365443266076 46-47 0.1342339469271346 48-49 0.15143588755197004 50-51 0.16105935083859127 52-53 0.16954671082054193 54-55 0.1659646439305218 56-57 0.1365729831426328 58-59 0.12879401698594733 60-61 0.12467731324667047 62-63 0.1213358329388159 64-65 0.1073016156458266 66-67 0.12101505082926187 68-69 0.11822157329189542 70-71 0.12632132155813494 72-73 0.15418926732564223 74-75 0.16794280027277173 76-77 0.1730352162619421 78-79 0.1696803700328561 80-81 0.17553464353221734 82-83 0.17580196195684572 84-85 0.15233140427447506 86-87 0.15400214442840238 88-89 0.174852981549415 90-91 0.2671446676523588 92-93 0.5438727008276445 94-95 0.17355648718996744 96-97 0.18558581629824394 98-99 0.2032288323237162 100-101 0.2107137482133105 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3740857.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.167796741877694 #Duplication Level Percentage of deduplicated Percentage of total 1 74.70358063404099 41.21231952309244 2 13.341358808715094 14.720267420393077 3 4.838109062956285 8.00723452200626 4 2.394347697882373 5.283635485046301 5 1.3488695819190186 3.720708146330499 6 0.8423281704017096 2.7881633576807543 7 0.5360453527261633 2.0700708744537573 8 0.39249463939952256 1.7322451590935553 9 0.27748832847073523 1.3777577732985224 >10 1.2213323328467687 11.461839792159958 >50 0.06545484461741924 2.484881077246264 >100 0.03643093843595548 3.646391041671064 >500 0.0015215417291341445 0.5547357782315289 >1k 5.889838696729812E-4 0.737400250460415 >5k 4.9081989139414886E-5 0.20234979883562848 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7473 0.19976705872477885 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4965 0.13272359782798435 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4941 0.13208203360887627 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 1.8712289723985708E-4 0.0 4 0.0 0.0 0.0 1.8712289723985708E-4 0.0 5 0.0 0.0 0.0 3.475139520168774E-4 0.0 6 0.0 0.0 0.0 3.475139520168774E-4 0.0 7 2.6731842462836724E-5 0.0 0.0 3.475139520168774E-4 0.0 8 2.6731842462836724E-5 0.0 0.0 3.475139520168774E-4 2.6731842462836724E-5 9 2.6731842462836724E-5 0.0 0.0 3.7424579447971416E-4 2.6731842462836724E-5 10-11 2.6731842462836724E-5 0.0 0.0 4.0097763694255085E-4 2.6731842462836724E-5 12-13 5.346368492567345E-5 0.0 0.0 4.945390855624794E-4 2.6731842462836724E-5 14-15 5.346368492567345E-5 0.0 0.0 5.480027704881528E-4 9.356144861992854E-5 16-17 5.346368492567345E-5 0.0 0.0 8.153211951165201E-4 2.138547397026938E-4 18-19 9.356144861992854E-5 0.0 0.0 9.62346328662122E-4 2.138547397026938E-4 20-21 1.3365921231418362E-4 0.0 0.0 0.0010425418560506324 2.138547397026938E-4 22-23 1.3365921231418362E-4 0.0 0.0 0.0014836172566874382 2.6731842462836725E-4 24-25 2.138547397026938E-4 0.0 0.0 0.0018979608148614073 3.207821095540407E-4 26-27 2.405865821655305E-4 0.0 0.0 0.0022989384518039583 3.341480307854591E-4 28-29 2.94050267091204E-4 2.6731842462836724E-5 0.0 0.00433055847897955 3.6087987324829576E-4 30-31 2.94050267091204E-4 2.6731842462836724E-5 0.0 0.00979722026262966 3.876117157111325E-4 32-33 2.94050267091204E-4 2.6731842462836724E-5 0.0 0.021104789624409592 4.277094794053876E-4 34-35 2.94050267091204E-4 2.6731842462836724E-5 0.0 0.03655579456792922 4.5444132186822433E-4 36-37 2.94050267091204E-4 2.6731842462836724E-5 0.0 0.06076147791802787 4.5444132186822433E-4 38-39 3.0741618832262233E-4 2.6731842462836724E-5 0.0 0.09647521944837774 4.5444132186822433E-4 40-41 3.207821095540407E-4 2.6731842462836724E-5 0.0 0.13758879315622063 4.5444132186822433E-4 42-43 3.207821095540407E-4 2.6731842462836724E-5 0.0 0.1818433583534468 4.5444132186822433E-4 44-45 3.475139520168774E-4 2.6731842462836724E-5 0.0 0.2331551299608619 4.6780724309964267E-4 46-47 3.7424579447971416E-4 2.6731842462836724E-5 0.0 0.2931012866837732 5.079050067938978E-4 48-49 3.7424579447971416E-4 2.6731842462836724E-5 0.0 0.3608531414058329 5.346368492567345E-4 50-51 3.7424579447971416E-4 2.6731842462836724E-5 0.0 0.4332697026376576 5.346368492567345E-4 52-53 3.7424579447971416E-4 2.6731842462836724E-5 0.0 0.5084396436431545 6.014664554138264E-4 54-55 3.7424579447971416E-4 4.0097763694255085E-5 0.0 0.5909474754047 6.682960615709181E-4 56-57 3.7424579447971416E-4 5.346368492567345E-5 0.0 0.691071591349255 6.682960615709181E-4 58-59 3.7424579447971416E-4 5.346368492567345E-5 0.0 0.7989613075292641 6.682960615709181E-4 60-61 3.7424579447971416E-4 5.346368492567345E-5 0.0 0.9178912746464246 6.682960615709181E-4 62-63 3.7424579447971416E-4 5.346368492567345E-5 0.0 1.0412052639274905 6.950279040337548E-4 64-65 3.7424579447971416E-4 5.346368492567345E-5 0.0 1.1700126468346692 7.083938252651731E-4 66-67 4.5444132186822433E-4 8.019552738851017E-5 0.0 1.3199916489724146 7.351256677280099E-4 68-69 4.5444132186822433E-4 1.069273698513469E-4 0.0 1.4910353429708754 7.484915889594283E-4 70-71 4.5444132186822433E-4 1.2029329108276525E-4 0.0 1.6809383518268675 7.618575101908467E-4 72-73 4.5444132186822433E-4 1.3365921231418362E-4 0.0 1.8774574916924118 8.554189588107752E-4 74-75 4.5444132186822433E-4 1.3365921231418362E-4 0.0 2.088612315306359 8.554189588107752E-4 76-77 4.5444132186822433E-4 1.3365921231418362E-4 0.0 2.3146567751721063 8.554189588107752E-4 78-79 4.5444132186822433E-4 1.3365921231418362E-4 0.0 2.5699325047709656 8.554189588107752E-4 80-81 5.747346129509896E-4 1.3365921231418362E-4 0.0 2.8495743087747005 8.687848800421935E-4 82-83 5.88100534182408E-4 1.3365921231418362E-4 0.0 3.132905107038307 9.088826437364487E-4 84-85 6.014664554138264E-4 1.3365921231418362E-4 0.0 3.4113439781312143 9.088826437364487E-4 86-87 6.28198297876663E-4 1.3365921231418362E-4 0.0 3.7169557670875952 9.088826437364487E-4 88-89 6.415642191080814E-4 1.3365921231418362E-4 0.0 4.050930040897046 9.088826437364487E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 14870 0.0 26.932749 1 TACATGG 10670 0.0 24.993824 2 GTACATG 10765 0.0 24.94891 1 TCAACGC 16525 0.0 24.091503 4 ATCAACG 16620 0.0 23.8691 3 CAACGCA 16690 0.0 23.796785 5 TATCAAC 16885 0.0 23.522594 2 ACATGGG 10840 0.0 23.37614 3 AACGCAG 17300 0.0 23.146303 6 GGTATCA 10555 0.0 21.35426 1 ACGCAGA 18910 0.0 20.748821 7 CGCAGAG 18875 0.0 20.486763 8 AGAGTAC 12165 0.0 20.008732 10-11 CATGGGG 6395 0.0 19.737152 4 GAGTACT 7530 0.0 19.6916 12-13 GCAGAGT 19935 0.0 17.969873 9 AGTACTT 8590 0.0 17.316908 12-13 GTACTTT 8545 0.0 16.770536 14-15 ATGGGGA 3870 0.0 16.55282 5 GTGTAGC 2455 0.0 16.235922 1 >>END_MODULE