FastQCFastQC Report
Fri 27 May 2016
SRR939587_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939587_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3740857
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76440.2043382037859239No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59300.1585198258046218No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT55450.14822806645642964No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41400.11066982779614405No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG105200.024.4746461
GTATCAA140650.024.0621261
TACATGG102700.023.6922762
GGTATCA102600.022.4798581
ACATGGG100100.022.424893
TCAACGC161450.020.724194
CAACGCA162450.020.5966175
ATCAACG162650.020.5712913
AACGCAG166000.020.2122466
TATCAAC169050.019.9335462
AGAGTAC127600.018.80025310-11
GAGTACT79350.018.4767812-13
ACGCAGA183700.017.983357
CGCAGAG182500.017.8443458
CATGGGG58950.017.5624394
AGTACTT89950.017.05692112-13
GTACTTT88050.016.67770814-15
CATGGGA48900.015.6864524
GCAGAGT197500.015.6796719
AGGACCG8350.015.2167995
Produced by FastQC (version 0.11.5)