##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939587_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3740857 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.529094536358915 31.0 31.0 34.0 30.0 34.0 2 31.747910973341135 31.0 31.0 34.0 30.0 34.0 3 31.798850905019894 31.0 31.0 34.0 30.0 34.0 4 35.58284986568586 37.0 35.0 37.0 33.0 37.0 5 35.31408658497237 37.0 35.0 37.0 33.0 37.0 6 35.3286899231914 37.0 35.0 37.0 32.0 37.0 7 35.260286613468516 37.0 35.0 37.0 32.0 37.0 8 35.22204403964118 37.0 35.0 37.0 32.0 37.0 9 36.83927693573959 39.0 37.0 39.0 33.0 39.0 10-11 36.718674357239536 39.0 35.0 39.0 32.0 39.0 12-13 36.661191807117994 39.0 35.0 39.0 32.0 39.0 14-15 37.74272726810995 40.0 37.0 41.0 32.0 41.0 16-17 37.696028877874774 39.0 37.0 41.0 32.0 41.0 18-19 37.660094464984894 39.0 36.5 41.0 32.0 41.0 20-21 37.58373910042538 39.0 36.0 41.0 32.0 41.0 22-23 37.44865294236054 39.0 36.0 41.0 32.0 41.0 24-25 37.32333606978294 39.0 36.0 41.0 31.5 41.0 26-27 37.06045045293097 39.0 36.0 40.0 31.0 41.0 28-29 36.86848949318298 39.0 36.0 40.0 30.5 41.0 30-31 36.65259230705691 38.5 35.5 40.0 30.0 41.0 32-33 36.506087776143275 38.0 35.0 40.0 30.0 41.0 34-35 36.50938501525185 38.0 35.0 40.0 30.0 41.0 36-37 36.429429406149445 38.0 35.0 40.0 30.0 41.0 38-39 36.48356927303021 39.0 35.0 40.0 30.0 41.0 40-41 36.40812426136578 39.0 35.0 40.0 30.0 41.0 42-43 36.24489228003102 38.5 35.0 40.0 30.0 41.0 44-45 36.06245026206562 38.0 35.0 40.0 29.0 41.0 46-47 35.82116103342095 38.0 35.0 40.0 28.5 41.0 48-49 35.567434414092816 38.0 34.0 40.0 27.5 41.0 50-51 35.3434678203417 38.0 34.0 40.0 27.0 41.0 52-53 35.11854529590413 38.0 34.0 40.0 27.0 41.0 54-55 34.82978058236388 38.0 34.0 40.0 26.0 41.0 56-57 32.70646699406045 37.0 30.5 39.5 16.5 41.0 58-59 27.485104883720496 32.5 17.5 38.5 7.5 40.0 60-61 28.262489985583514 33.0 20.0 38.0 9.5 40.0 62-63 31.47745757188794 34.5 29.5 38.5 15.0 40.0 64-65 31.74627952899563 35.5 31.0 39.0 10.5 40.0 66-67 24.902360608812366 27.0 18.0 35.5 2.0 39.0 68-69 17.270516087623772 13.0 7.5 28.0 2.0 35.0 70-71 16.115369953997174 12.5 7.5 26.0 2.0 33.5 72-73 17.18314867422091 13.0 7.0 28.0 2.0 34.0 74-75 18.212166757510378 17.0 8.5 29.0 2.0 34.5 76-77 19.466747325545988 21.0 8.0 30.0 2.0 34.0 78-79 22.565076799246803 27.5 10.5 33.5 2.0 35.5 80-81 26.30156191482326 31.5 22.5 34.5 2.0 36.0 82-83 27.659549937353926 33.0 26.5 35.0 2.0 36.0 84-85 27.727799271664217 34.0 27.0 35.0 2.0 36.0 86-87 27.4903963182768 34.0 27.0 35.0 2.0 36.0 88-89 27.053704004189413 33.0 26.0 35.0 2.0 35.5 90-91 25.635930884286676 32.5 18.5 35.0 2.0 35.0 92-93 23.036353835498122 31.5 2.0 34.0 2.0 35.0 94-95 15.537486196344847 4.5 2.0 32.0 2.0 34.5 96-97 12.948515941667912 2.0 2.0 29.5 2.0 34.0 98-99 12.569994656304692 2.0 2.0 29.0 2.0 34.0 100-101 12.269289497032364 2.0 2.0 30.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.36811586727483814 1101 2 0.41363364492728394 1101 3 0.4060035149778294 1101 4 0.28148937416993647 1101 5 0.4300262722458825 1101 6 0.4722089239413876 1101 7 0.4579329854447778 1101 8 0.4068570922477974 1101 9 0.5238932969991055 1101 10-11 0.45521977751236165 1101 12-13 0.5687965110664237 1101 14-15 0.6763186138948143 1101 16-17 0.6344809776564162 1101 18-19 0.6678360092147528 1101 20-21 0.5391171680934406 1101 22-23 0.563943675257164 1101 24-25 0.48979279226905703 1101 26-27 0.4574103335225672 1101 28-29 0.5391805113174897 1101 30-31 0.5435048171486585 1101 32-33 0.6081895953385796 1101 34-35 0.5423297152527766 1101 36-37 0.30675059945571803 1101 38-39 0.5282541405615362 1101 40-41 0.562627766353593 1101 42-43 0.47728519904133293 1101 44-45 0.43607214489293966 1101 46-47 0.508005937934918 1101 48-49 0.348523028625074 1101 50-51 0.20535327302252426 1101 52-53 0.33942264070362427 1101 54-55 0.19348464063431692 1101 56-57 -2.2844266228354506 1101 58-59 -9.912648278608213 1101 60-61 -10.348691984597597 1101 62-63 -3.7561705837414827 1101 64-65 -5.591698859092176 1101 66-67 -9.968137821660003 1101 68-69 -5.213984915555287 1101 70-71 -4.77388499986974 1101 72-73 -5.173515514406789 1101 74-75 -7.025978447476636 1101 76-77 -8.115668615836833 1101 78-79 -11.326471493892612 1101 80-81 -8.215520181445736 1101 82-83 -4.5505910130237375 1101 84-85 -3.75449536429765 1101 86-87 -3.5317234285450603 1101 88-89 -3.6338925505784196 1101 90-91 -9.854442852858387 1101 92-93 -16.773646459798396 1101 94-95 -11.1145042810111 1101 96-97 -9.273136016201903 1101 98-99 -9.290054175679488 1101 100-101 -9.072751400038777 1102 1 0.3554047862936969 1102 2 0.35463804365386054 1102 3 0.41172789796710063 1102 4 0.2582827610150886 1102 5 0.28487249734664033 1102 6 0.3347583675952279 1102 7 0.4593137348841978 1102 8 0.4164630432903351 1102 9 0.4335214408809165 1102 10-11 0.4272399141679628 1102 12-13 0.3473854324508565 1102 14-15 0.4217038728693794 1102 16-17 0.43200929298023993 1102 18-19 0.36265956836100344 1102 20-21 0.5139928041532826 1102 22-23 0.538122322321918 1102 24-25 0.48652534023111116 1102 26-27 0.4649951176388427 1102 28-29 0.5393788617639004 1102 30-31 0.4630035609032248 1102 32-33 0.478164924869553 1102 34-35 0.23463588118922019 1102 36-37 0.26009700259720603 1102 38-39 0.17460945694489283 1102 40-41 0.23595975504364475 1102 42-43 0.3377385399196058 1102 44-45 0.354909227664443 1102 46-47 0.46423118498199756 1102 48-49 0.32648683095255393 1102 50-51 0.38052387463834236 1102 52-53 0.12030430337993891 1102 54-55 0.05182584884610009 1102 56-57 -2.95145474019791 1102 58-59 -10.462961203867213 1102 60-61 -10.811703089109997 1102 62-63 -4.662193791910937 1102 64-65 -5.817149392405092 1102 66-67 -7.276829555340953 1102 68-69 -4.708919236578909 1102 70-71 -4.551437114134538 1102 72-73 -4.783612151304549 1102 74-75 -5.473775317943913 1102 76-77 -7.792774087538941 1102 78-79 -10.504294886826013 1102 80-81 -6.254317419822353 1102 82-83 -3.767922095324618 1102 84-85 -3.327784025593214 1102 86-87 -3.1652293866843273 1102 88-89 -3.3852811593332923 1102 90-91 -9.447380503119161 1102 92-93 -13.770992112180718 1102 94-95 -10.71094811974355 1102 96-97 -9.189412829696145 1102 98-99 -9.331538677420676 1102 100-101 -9.17090841390977 1103 1 0.24560403772607842 1103 2 0.2514271073209251 1103 3 0.2700173080407673 1103 4 0.24283002699066714 1103 5 0.23903963941569373 1103 6 0.31681227058020767 1103 7 0.17354945840349956 1103 8 0.2735657607883937 1103 9 0.41542125468001245 1103 10-11 0.4109849018584697 1103 12-13 0.35651962766421263 1103 14-15 0.49613718342260427 1103 16-17 0.45564625062453956 1103 18-19 0.4044130327211164 1103 20-21 0.2874510448076535 1103 22-23 0.3962680824579934 1103 24-25 0.3658321917891172 1103 26-27 0.40278219696654816 1103 28-29 0.4502136399283998 1103 30-31 0.4097971384424355 1103 32-33 0.4615384370561273 1103 34-35 0.4408775665485223 1103 36-37 0.2686485848343665 1103 38-39 0.33871701296101975 1103 40-41 0.3888171096263946 1103 42-43 0.34758311862683655 1103 44-45 0.21379411598507403 1103 46-47 0.21528885727076386 1103 48-49 0.2522550272974655 1103 50-51 0.28921750416793657 1103 52-53 0.21397141427827648 1103 54-55 0.1885508890445564 1103 56-57 -2.8318292621979637 1103 58-59 -10.653283035599468 1103 60-61 -10.606595973600744 1103 62-63 -4.432303092310747 1103 64-65 -5.588648583142806 1103 66-67 -6.852114987913993 1103 68-69 -4.308595605179061 1103 70-71 -4.103872134960103 1103 72-73 -4.654189512309632 1103 74-75 -5.240926708804661 1103 76-77 -7.087146259384847 1103 78-79 -9.404080970455272 1103 80-81 -5.357477703746383 1103 82-83 -3.174105894317041 1103 84-85 -2.592146372783205 1103 86-87 -2.339081686906521 1103 88-89 -2.4367826025961286 1103 90-91 -5.552552915391249 1103 92-93 -10.603555759248259 1103 94-95 -8.62408026768799 1103 96-97 -8.197900526674468 1103 98-99 -8.494949156308694 1103 100-101 -8.440973457778137 1104 1 0.1737573353839501 1104 2 0.22696735368808874 1104 3 0.16035877528650033 1104 4 0.1500229880012185 1104 5 0.21946536349688017 1104 6 0.21288843424988357 1104 7 0.22083418165054525 1104 8 0.24116579955046547 1104 9 0.2427630189749479 1104 10-11 0.23176387381487018 1104 12-13 0.3418548974121265 1104 14-15 0.40764334323507256 1104 16-17 0.3539377743349519 1104 18-19 0.32363056457790407 1104 20-21 0.2577709522347078 1104 22-23 0.38843307714087416 1104 24-25 0.4491581991754501 1104 26-27 0.42811887912326085 1104 28-29 0.4849990202938983 1104 30-31 0.48004778716876473 1104 32-33 0.4577586917584071 1104 34-35 0.40450392298349414 1104 36-37 0.28967951025347816 1104 38-39 0.28263728976780556 1104 40-41 0.17915917857094854 1104 42-43 0.3309672374515813 1104 44-45 0.19949578864444817 1104 46-47 0.26829576794002463 1104 48-49 0.28844638778915765 1104 50-51 0.2253536350418699 1104 52-53 0.2715383152696873 1104 54-55 0.29354806106011466 1104 56-57 -2.880104983397331 1104 58-59 -10.506802403651037 1104 60-61 -10.637644360952905 1104 62-63 -4.363866913870943 1104 64-65 -5.683806301070199 1104 66-67 -6.672787490848066 1104 68-69 -4.2411724342009585 1104 70-71 -4.128513316620577 1104 72-73 -4.4571236807587 1104 74-75 -5.2031348927952905 1104 76-77 -6.918848218326186 1104 78-79 -9.48954304031187 1104 80-81 -5.281431903699133 1104 82-83 -2.87757801317726 1104 84-85 -2.3582289904644114 1104 86-87 -2.2842980229660768 1104 88-89 -2.28016271220665 1104 90-91 -4.7273007877525615 1104 92-93 -6.8915627300874025 1104 94-95 -7.975497300071419 1104 96-97 -8.044461469580849 1104 98-99 -8.324123346849607 1104 100-101 -8.294996416071541 1105 1 0.2804960958245495 1105 2 0.2860079402542688 1105 3 0.3203951170000643 1105 4 0.202285923030324 1105 5 0.2502393418414357 1105 6 0.21751862089463003 1105 7 0.2609099607569263 1105 8 0.21018487895008775 1105 9 0.3633272390361242 1105 10-11 0.34358231222678626 1105 12-13 0.33425757374390486 1105 14-15 0.5010924371960996 1105 16-17 0.45943068646514007 1105 18-19 0.4577461855965268 1105 20-21 0.44324112803157334 1105 22-23 0.530049249333473 1105 24-25 0.5042345278299294 1105 26-27 0.5699292141394849 1105 28-29 0.542705213018138 1105 30-31 0.6521606597965288 1105 32-33 0.6136845437368095 1105 34-35 0.44463881915793024 1105 36-37 0.458237099317671 1105 38-39 0.4351655362150879 1105 40-41 0.5125536559051724 1105 42-43 0.45560234076337736 1105 44-45 0.49458533246750136 1105 46-47 0.662455827564365 1105 48-49 0.44440030249018747 1105 50-51 0.5679557095371734 1105 52-53 0.7033198851822888 1105 54-55 0.5980195941432385 1105 56-57 1.7833381013039684 1105 58-59 -8.600052847354224 1105 60-61 -9.906206339898386 1105 62-63 -3.685278511091859 1105 64-65 -4.9570500721538195 1105 66-67 -6.2779714528882735 1105 68-69 -3.983866078368237 1105 70-71 -4.149203835192376 1105 72-73 -2.318418097415016 1105 74-75 -4.522836224782829 1105 76-77 -6.47310354162631 1105 78-79 -9.148758929470945 1105 80-81 -4.74721398218761 1105 82-83 -2.2743813991739934 1105 84-85 -1.689116130193355 1105 86-87 -1.5270941302144543 1105 88-89 -1.5778380586983545 1105 90-91 -4.467867550693118 1105 92-93 -7.0534170354553485 1105 94-95 -8.3214352941363 1105 96-97 -8.251944005982722 1105 98-99 -8.459686425541644 1105 100-101 -8.396346255792098 1106 1 0.2481076138306122 1106 2 0.2651994265451272 1106 3 0.26463891346783797 1106 4 0.14942160101588087 1106 5 0.12649095077519235 1106 6 0.22624185691533683 1106 7 0.25261878060673126 1106 8 0.23657481419459003 1106 9 0.2696950706879946 1106 10-11 0.268668210159575 1106 12-13 0.23329344215753878 1106 14-15 0.252314029289316 1106 16-17 0.39089679605242367 1106 18-19 0.23671531397590684 1106 20-21 0.35908370541125123 1106 22-23 0.4246098090091266 1106 24-25 0.41761595757714787 1106 26-27 0.3041219259883974 1106 28-29 0.4066353754947869 1106 30-31 0.4447468578977194 1106 32-33 0.43211539706737057 1106 34-35 0.5338312168409374 1106 36-37 0.47935876799271426 1106 38-39 0.4727966227127425 1106 40-41 0.38850486314206023 1106 42-43 0.3372962517306277 1106 44-45 0.48605782802096087 1106 46-47 0.5092702475923474 1106 48-49 0.5952650985529786 1106 50-51 0.5245350277163112 1106 52-53 0.5185169294100049 1106 54-55 0.4427104393490353 1106 56-57 1.8650288550594922 1106 58-59 -8.687130690819977 1106 60-61 -10.01824831868317 1106 62-63 -3.726806887737574 1106 64-65 -5.258130491779923 1106 66-67 -7.002281236636382 1106 68-69 -4.311554638561489 1106 70-71 -4.10870116353804 1106 72-73 -4.5660824606131865 1106 74-75 -5.141569392800616 1106 76-77 -6.802333692096223 1106 78-79 -9.530062543747391 1106 80-81 -5.1058034022000385 1106 82-83 -2.515725662162577 1106 84-85 -1.9873817322627403 1106 86-87 -1.871772144387382 1106 88-89 -1.838044251063689 1106 90-91 -4.529978925924777 1106 92-93 -6.759247021314772 1106 94-95 -7.612717237187043 1106 96-97 -7.297205435555748 1106 98-99 -6.889595314321318 1106 100-101 -7.427533908636917 1107 1 0.054270744914798996 1107 2 0.03131430672461022 1107 3 0.04047250931273183 1107 4 0.06423347627038822 1107 5 -0.04032917630600963 1107 6 -0.004031590488871473 1107 7 -0.018273937962952402 1107 8 0.16646117870404709 1107 9 0.04976183826154568 1107 10-11 0.026273797429944068 1107 12-13 0.05223634013146494 1107 14-15 0.1101042070032392 1107 16-17 0.01797485034237667 1107 18-19 0.06772900555427697 1107 20-21 0.040491894235174186 1107 22-23 0.0627386650477959 1107 24-25 0.15560505502399025 1107 26-27 0.06346735021987371 1107 28-29 0.13866879001462706 1107 30-31 0.18266334848956234 1107 32-33 0.19309720309566814 1107 34-35 0.18968797773004553 1107 36-37 0.05014119329217692 1107 38-39 -0.07951899813349428 1107 40-41 -0.02597106134680871 1107 42-43 -0.0791875404216853 1107 44-45 -0.011526130858200645 1107 46-47 0.06516262041510146 1107 48-49 -0.029034917581881814 1107 50-51 -0.06170124805144184 1107 52-53 -0.09194229934384879 1107 54-55 -0.07130844611653231 1107 56-57 1.188821852531703 1107 58-59 -8.874214392309764 1107 60-61 -10.507121875189739 1107 62-63 -4.087043916465301 1107 64-65 -5.529407219938321 1107 66-67 -7.132348618871134 1107 68-69 -4.10785414760085 1107 70-71 -4.034408369672359 1107 72-73 -2.414094146859373 1107 74-75 -4.789691948561913 1107 76-77 -6.92440817366791 1107 78-79 -7.893183332357719 1107 80-81 -2.6574817346241844 1107 82-83 -1.4468704686595437 1107 84-85 -1.1730081488647421 1107 86-87 -1.2365581654548912 1107 88-89 -1.0477398401012046 1107 90-91 -0.542907248744843 1107 92-93 -3.671064420836874 1107 94-95 -6.111468898693117 1107 96-97 -6.541092821939466 1107 98-99 -6.140584419451434 1107 100-101 -7.0200286648085 1108 1 -0.1481817552206195 1108 2 -0.12976750806813797 1108 3 -0.14897546308110776 1108 4 -0.04736391478182611 1108 5 -0.13085424775893273 1108 6 -0.2615514969389139 1108 7 -0.2749357083385817 1108 8 -0.19992902276611346 1108 9 -0.20217521306666697 1108 10-11 -0.1117134764617731 1108 12-13 -0.17357879673633647 1108 14-15 -0.16916533222497065 1108 16-17 -0.21595593670720348 1108 18-19 -0.23256222286247663 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2316 78-79 7.863459656467388 2316 80-81 3.811170375849109 2316 82-83 2.043880669362178 2316 84-85 1.6300932115189966 2316 86-87 1.4517874436597644 2316 88-89 1.6998313026386107 2316 90-91 2.840451509872235 2316 92-93 5.054705283758025 2316 94-95 11.839040917563336 2316 96-97 13.819674479922433 2316 98-99 13.607597454330817 2316 100-101 12.309115350638889 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9.0 3 16.0 4 58.0 5 137.0 6 259.0 7 687.0 8 1581.0 9 2860.0 10 4681.0 11 7362.0 12 11282.0 13 15598.0 14 19691.0 15 27567.0 16 38120.0 17 51121.0 18 58763.0 19 63057.0 20 67489.0 21 67431.0 22 67536.0 23 57733.0 24 57408.0 25 67299.0 26 95013.0 27 141904.0 28 202050.0 29 273913.0 30 339205.0 31 368376.0 32 366643.0 33 330043.0 34 289069.0 35 256724.0 36 207639.0 37 131421.0 38 48085.0 39 3027.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.58162596852278 20.0602998844377 14.300821342608264 24.05725280443126 2 15.376146945959803 21.16430420260355 39.735405094282015 23.72414375715463 3 18.759521573590153 25.301997114557214 30.443747097916596 25.49473421393604 4 12.720721481735334 17.50705787470625 38.13631475354444 31.635905890013973 5 12.997379788284324 38.15925576136049 35.48397890981204 13.359385540543144 6 31.294727384660785 37.77359038316621 17.63315197560345 13.298530256569553 7 27.33424453273675 32.785856289080286 22.08197212563859 17.797927052544377 8 25.22719161887144 36.266051109593306 20.167638610612478 18.339118660922775 9 25.539575557151746 16.559280400186374 20.477767527601294 37.42337651506059 10-11 24.228124731846204 26.671428498870714 28.832056397771954 20.268390371511128 12-13 25.23126385210662 24.00340349818237 28.613657244850575 22.151675404860438 14-15 22.22811812902234 25.580119358697612 26.48752614730708 25.704236364972964 16-17 21.911195750064756 28.525415432880752 27.130681552382246 22.43270726467224 18-19 21.824089562472725 27.887119313044284 28.600258898205688 21.6885322262773 20-21 22.697272309526934 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22.368058500569614 46-47 22.51619074177804 27.25755770899833 27.86231359470999 22.363937954513645 48-49 22.310147776395787 27.30383514772622 28.026545824599246 22.35947125127875 50-51 22.12226817598644 27.394445654693055 28.028992085330913 22.454294083989588 52-53 22.45005557964371 27.51999736717442 27.62949776042598 22.40044929275589 54-55 22.41034450977961 27.257564430133403 27.775394535790166 22.55669652429683 56-57 21.524999741230644 27.29776099309361 28.77438632342782 22.40285294224792 58-59 21.29799910052093 26.70283327757432 29.95577719705098 22.04339042485377 60-61 22.232397106612247 27.200368392028455 28.41216800912434 22.15506649223496 62-63 22.24958356490894 27.261815301446607 28.049118889912766 22.439482243731685 64-65 21.814489035285227 27.444138629313297 28.24149578705346 22.499876548348016 66-67 21.95476480240006 26.764651415298164 29.048772846835362 22.231810935466413 68-69 22.27672561410598 26.104829204837888 29.731044365216558 21.88740081583957 70-71 22.19326785233528 26.0508616690312 29.662509869037084 22.09336060959643 72-73 21.46396659872834 26.252630070041832 29.982331641191166 22.30107169003866 74-75 21.80913617698057 26.75334139167826 29.193775822045993 22.24374660929518 76-77 20.017336287083705 27.29180996044549 29.657960914158256 23.032892838312545 78-79 20.877656236800963 27.3150353454211 28.967038738408803 22.84026967936914 80-81 21.947272378346465 27.346520922475943 27.755575571198072 22.95063112797953 82-83 22.25878701048706 27.31392356573649 27.63842213872067 22.788867285055783 84-85 22.225946862921372 27.33467473862245 27.451573210708897 22.987805187747288 86-87 21.98444601717394 27.29951875814071 27.610117239632515 23.10591798505283 88-89 22.188251959078322 27.294827236618595 27.35764712518146 23.159273679121622 90-91 21.523356115298792 27.298881841624695 27.99102407355693 23.186737969519587 92-93 20.556844497743786 27.49368710591383 28.693740894348135 23.255727501994254 94-95 19.960446134426117 27.146074141738826 29.480344277151538 23.41313544668352 96-97 19.948208249854233 27.14241717484798 29.24152842381226 23.667846151485524 98-99 20.13677803305458 27.35441310899114 28.707233977914786 23.801574880039496 100-101 20.51462748137363 27.292940582524377 28.33530745166806 23.857124484433932 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 288.0 1 240.0 2 182.0 3 190.0 4 227.0 5 282.0 6 354.0 7 481.5 8 691.5 9 868.5 10 1015.5 11 1208.0 12 1433.5 13 1668.5 14 1918.5 15 2213.5 16 2572.0 17 2924.5 18 3349.0 19 3910.0 20 4675.5 21 5749.5 22 7133.5 23 8955.5 24 11349.5 25 14585.0 26 18905.0 27 24136.0 28 29963.0 29 36757.5 30 44511.5 31 53140.5 32 62552.5 33 72503.5 34 82670.5 35 92659.5 36 102209.0 37 111203.5 38 118395.5 39 124420.0 40 129885.5 41 133945.0 42 138043.0 43 141938.0 44 145081.5 45 148418.0 46 151711.0 47 153640.0 48 153633.5 49 151099.5 50 145537.0 51 138467.0 52 130690.5 53 122778.5 54 114465.5 55 104848.0 56 94181.0 57 82571.0 58 71103.5 59 59540.5 60 47511.0 61 36640.5 62 27437.5 63 20093.5 64 14479.0 65 10043.5 66 6914.0 67 4728.5 68 3258.0 69 2229.5 70 1438.5 71 902.0 72 541.5 73 290.0 74 173.0 75 106.0 76 60.5 77 35.0 78 19.5 79 8.0 80 5.0 81 4.0 82 2.5 83 3.0 84 1.5 85 0.0 86 0.5 87 1.0 88 1.5 89 1.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023604216894684827 2 0.007244329307428752 3 2.6731842462836725E-4 4 0.0 5 0.14117086004624074 6 0.0 7 0.0 8 0.001924692657324244 9 0.0 10-11 0.0 12-13 0.0 14-15 8.554189588107752E-4 16-17 0.0 18-19 1.2029329108276525E-4 20-21 0.0 22-23 4.277094794053876E-4 24-25 1.3365921231418362E-5 26-27 3.341480307854591E-4 28-29 0.004023142290656927 30-31 0.014141144662840627 32-33 0.0031677233318461516 34-35 0.005159245595327488 36-37 0.11113763503924368 38-39 0.11104407359062375 40-41 0.1231535982262888 42-43 0.12609410089720083 44-45 0.12166998096960135 46-47 0.1151607773299006 48-49 0.1149335566689665 50-51 0.11363706230951892 52-53 0.09162339004137288 54-55 0.07889903302906259 56-57 0.570150101968613 58-59 1.5995666233699926 60-61 0.5298518494558867 62-63 0.0047582679583849374 64-65 0.12233827703117227 66-67 1.914334068369895 68-69 2.891249251174263 70-71 2.7930498278870326 72-73 2.654979861566481 74-75 2.0184011310777183 76-77 1.4379860016033759 78-79 0.9507981727181767 80-81 0.05652448088766825 82-83 2.2722066093411216E-4 84-85 1.069273698513469E-4 86-87 3.876117157111325E-4 88-89 9.356144861992854E-5 90-91 0.25510197262285084 92-93 0.4049606814695135 94-95 2.024362331946931 96-97 2.4390400381516857 98-99 1.449587621232247 100-101 0.9577350858372827 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3740857.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.21473131826216 #Duplication Level Percentage of deduplicated Percentage of total 1 69.84884144735788 34.37591964723612 2 15.227992277322077 14.988830968899544 3 5.948196291797322 8.782166469872656 4 2.94527016439707 5.798027192019825 5 1.6657128708226703 4.098880569545442 6 1.018646764995124 3.007945610847118 7 0.7114055392856516 2.450814273198672 8 0.4908298004308112 1.9324845400958888 9 0.3740767984935061 1.6569080217228238 >10 1.6303142031301632 13.581670917311106 >50 0.08541479143691838 2.911590096198728 >100 0.0497601133894169 4.427747807666706 >500 0.002613368983478045 0.8244837641627124 >1k 7.622326006602607E-4 0.6502355241715546 >5k 1.633355572843412E-4 0.5122945970509719 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7644 0.2043382037859239 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5930 0.1585198258046218 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5545 0.14822806645642964 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4140 0.11066982779614405 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.6731842462836724E-5 2 0.0 0.0 0.0 0.0 2.6731842462836724E-5 3 0.0 0.0 0.0 1.3365921231418362E-4 2.6731842462836724E-5 4 0.0 0.0 0.0 1.3365921231418362E-4 2.6731842462836724E-5 5 5.346368492567345E-5 0.0 0.0 2.138547397026938E-4 2.6731842462836724E-5 6 5.346368492567345E-5 0.0 0.0 2.138547397026938E-4 2.6731842462836724E-5 7 5.346368492567345E-5 0.0 0.0 2.405865821655305E-4 2.6731842462836724E-5 8 8.019552738851017E-5 0.0 0.0 2.405865821655305E-4 2.6731842462836724E-5 9 8.019552738851017E-5 0.0 0.0 3.7424579447971416E-4 5.346368492567345E-5 10-11 8.019552738851017E-5 0.0 0.0 4.5444132186822433E-4 5.346368492567345E-5 12-13 8.019552738851017E-5 0.0 0.0 5.480027704881528E-4 6.682960615709181E-5 14-15 1.069273698513469E-4 0.0 0.0 6.014664554138263E-4 1.3365921231418362E-4 16-17 1.069273698513469E-4 0.0 0.0 8.019552738851017E-4 2.6731842462836725E-4 18-19 1.069273698513469E-4 0.0 0.0 9.356144861992853E-4 2.94050267091204E-4 20-21 1.069273698513469E-4 0.0 0.0 0.001029175934819214 3.207821095540407E-4 22-23 1.3365921231418362E-4 0.0 0.0 0.0013766898868360913 4.1434355817396924E-4 24-25 1.3365921231418362E-4 0.0 0.0 0.0017910334450100605 5.212709280253162E-4 26-27 1.3365921231418362E-4 0.0 0.0 0.0021251814757955196 5.346368492567345E-4 28-29 2.138547397026938E-4 0.0 0.0 0.004009776369425509 5.346368492567345E-4 30-31 2.138547397026938E-4 0.0 0.0 0.009596731444158384 5.346368492567345E-4 32-33 2.138547397026938E-4 0.0 0.0 0.02145230357642647 5.88100534182408E-4 34-35 2.539525033969489E-4 0.0 0.0 0.03735774984181432 6.148323766452447E-4 36-37 3.0741618832262233E-4 0.0 0.0 0.06241885215072375 6.148323766452447E-4 38-39 3.7424579447971416E-4 4.0097763694255085E-5 0.0 0.09929542882820702 6.148323766452447E-4 40-41 3.7424579447971416E-4 8.019552738851017E-5 0.0 0.1412911533373235 6.415642191080814E-4 42-43 3.7424579447971416E-4 8.019552738851017E-5 0.0 0.18688231065769154 6.415642191080814E-4 44-45 3.7424579447971416E-4 8.019552738851017E-5 0.0 0.23995838386765383 6.415642191080814E-4 46-47 4.0097763694255085E-4 8.019552738851017E-5 0.0 0.29922287860776287 6.415642191080814E-4 48-49 4.0097763694255085E-4 1.069273698513469E-4 0.0 0.35530628409479437 6.415642191080814E-4 50-51 4.0097763694255085E-4 1.6039105477702034E-4 0.0 0.4099060723251383 6.415642191080814E-4 52-53 4.0097763694255085E-4 1.8712289723985708E-4 0.0 0.4649603018773506 6.950279040337548E-4 54-55 4.277094794053876E-4 1.8712289723985708E-4 0.0 0.5260826596686268 7.618575101908467E-4 56-57 4.277094794053876E-4 2.0048881847127542E-4 0.0 0.5775013586458931 7.75223431422265E-4 58-59 4.277094794053876E-4 2.539525033969489E-4 0.0 0.6126537314845235 8.019552738851017E-4 60-61 4.277094794053876E-4 2.6731842462836725E-4 0.0 0.6406286046218821 8.019552738851017E-4 62-63 4.81173164331061E-4 2.806843458597856E-4 0.0 0.6665852236532965 8.019552738851017E-4 64-65 4.81173164331061E-4 3.475139520168774E-4 0.0 0.6917265214895945 8.019552738851017E-4 66-67 4.81173164331061E-4 3.475139520168774E-4 0.0 0.7141545373159145 8.153211951165201E-4 68-69 4.81173164331061E-4 3.475139520168774E-4 0.0 0.746406505247327 8.286871163479385E-4 70-71 5.079050067938978E-4 3.475139520168774E-4 0.0 0.7957534864337237 8.286871163479385E-4 72-73 5.079050067938978E-4 3.475139520168774E-4 0.0 0.8671275058094977 9.088826437364487E-4 74-75 5.079050067938978E-4 3.475139520168774E-4 0.0 0.9645249738228432 9.088826437364487E-4 76-77 5.079050067938978E-4 3.475139520168774E-4 0.0 1.119623123792222 9.088826437364487E-4 78-79 5.079050067938978E-4 3.475139520168774E-4 0.0 1.3563202228794098 9.088826437364487E-4 80-81 5.613686917195712E-4 3.475139520168774E-4 0.0 1.645171146611592 9.356144861992853E-4 82-83 5.613686917195712E-4 3.475139520168774E-4 0.0 1.9372699892030087 0.0010960055409763058 84-85 5.613686917195712E-4 3.475139520168774E-4 0.0 2.158609644795297 0.0010960055409763058 86-87 5.613686917195712E-4 3.475139520168774E-4 0.0 2.3279291349549047 0.0010960055409763058 88-89 5.613686917195712E-4 3.7424579447971416E-4 0.0 2.494562074946997 0.0010960055409763058 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 10520 0.0 24.474646 1 GTATCAA 14065 0.0 24.062126 1 TACATGG 10270 0.0 23.692276 2 GGTATCA 10260 0.0 22.479858 1 ACATGGG 10010 0.0 22.42489 3 TCAACGC 16145 0.0 20.72419 4 CAACGCA 16245 0.0 20.596617 5 ATCAACG 16265 0.0 20.571291 3 AACGCAG 16600 0.0 20.212246 6 TATCAAC 16905 0.0 19.933546 2 AGAGTAC 12760 0.0 18.800253 10-11 GAGTACT 7935 0.0 18.47678 12-13 ACGCAGA 18370 0.0 17.98335 7 CGCAGAG 18250 0.0 17.844345 8 CATGGGG 5895 0.0 17.562439 4 AGTACTT 8995 0.0 17.056921 12-13 GTACTTT 8805 0.0 16.677708 14-15 CATGGGA 4890 0.0 15.686452 4 GCAGAGT 19750 0.0 15.679671 9 AGGACCG 835 0.0 15.216799 5 >>END_MODULE