FastQCFastQC Report
Fri 27 May 2016
SRR939586_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939586_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3487687
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA120150.028.9118271
GTACATG84000.027.8113331
TACATGG94200.025.5274942
ACATGGG94400.024.372753
GGTATCA89850.023.9657481
TATCAAC152100.023.3266322
ATCAACG157850.022.7466033
CAACGCA159350.022.6302645
TCAACGC160200.022.352024
AACGCAG165050.021.9631186
ACGCAGA170700.020.9872997
GAGTACT68850.020.18998112-13
CATGGGG58250.019.9502944
AGAGTAC114050.019.92705310-11
CGCAGAG178350.019.663088
GTATAGG10750.018.8970831
GTATAGT13850.018.3342651
GTACTTT74850.017.94273614-15
GCAGAGT184500.017.5236199
GAGTACA70900.017.191281
Produced by FastQC (version 0.11.5)