FastQCFastQC Report
Fri 27 May 2016
SRR939586_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939586_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3487687
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72750.20859096587509143No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54490.15623535024788635No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT49700.14250131964250234No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38960.11170727189681873No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA134450.023.6242181
TACATGG97650.023.2398172
GTACATG101200.023.038261
GGTATCA99150.022.2293831
ACATGGG95100.022.1745973
ATCAACG154150.020.1683623
TCAACGC154350.020.142234
CAACGCA156100.019.946645
AACGCAG158500.019.6024746
TATCAAC160750.019.5177882
AGAGTAC115850.018.90413710-11
CATGGGG56450.018.5531544
GTGTAGC20800.017.698421
ACGCAGA172800.017.6255827
GAGTACT73500.017.38422412-13
CGCAGAG172300.017.2936448
AGTACTT80250.016.5682812-13
GAGTACA80400.016.2602391
GTACTTT79350.015.89462414-15
GCAGAGT181900.015.785269
Produced by FastQC (version 0.11.5)