FastQCFastQC Report
Fri 27 May 2016
SRR939585_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939585_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2260283
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134990.5972260995636387No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT122190.5405960227104305No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88790.39282691592159036No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT67230.29744063022196776No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65770.2909812620808987No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT54730.24213782079500665No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC44550.19709921279768952No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA36940.16343086241855556No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT36530.16161693026935123No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT31050.13737217861657147No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC30150.13339037633783027No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC28410.12569222526559726No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA27000.11945406836223606No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA23850.10551776038664185No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC23500.10396928172268693No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG111950.029.9686741
TACATGG117200.028.3023012
ACATGGG116100.027.4662653
CATGGGG31350.025.430424
CATGGGA84250.024.2766744
GAGTACT103950.023.16697512-13
AGTACTT106900.022.54985612-13
ATGGGAT31650.022.3405765
TAGCCCG1353.8236164E-421.08979
CGTATAC1601.6370905E-1120.90715490-91
GTACTTT115650.020.90529814-15
CATTCCG5450.020.0255419
ATGGGAG32750.019.4166955
CTTTGCG3951.8189894E-1219.2218462
GTCGGGA4200.019.2075422
GTATATG10200.019.074621
AGAGTAC201600.018.28872710-11
ACTTTTT137850.018.22848716-17
CCCTATA3951.8189894E-1118.0204812
AGTACAT79550.017.7765832
Produced by FastQC (version 0.11.5)