FastQCFastQC Report
Fri 27 May 2016
SRR939585_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939585_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2260283
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140690.622444180662333No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT137300.6074460587457411No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104730.46334905850285124No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78710.3482307303996889No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT75280.33305563949293077No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT59440.26297591938708564No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC49130.21736216217172807No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT38350.16966901932191678No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA38050.16834175189566972No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT37630.1664835774989238No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC35220.1558211958414057No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC34250.15152969782987352No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA31500.13936307975594206No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC29180.12909887832629807No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA27260.12060436679831685No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG27090.11985224859011018No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24970.11047289211129757No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG115600.026.7610361
TACATGG117000.026.1153892
ACATGGG113400.025.6142083
CATGGGA82800.023.8796394
GAGTACT103600.022.71057912-13
CGTACGA652.51584E-422.43766268-69
ATGGGAT32850.022.3562035
AGTACTT106400.021.67111612-13
CATGGGG30350.021.095464
TGCACCG5450.020.7311575
GTACTTT113700.020.71386714-15
CATTCCG5000.020.6840979
GCACCGT5900.019.1228566
ACTTTTT129050.018.54144916-17
ATGGGAG33850.018.0797945
TACTTTT131600.017.55704914-15
AGAGTAC204650.017.43467710-11
CGCGGAA3950.017.25658482-83
GTATCAA291850.017.182461
TGGGATA11450.016.8334036
Produced by FastQC (version 0.11.5)