##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939585_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2260283 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.54988866438406 31.0 31.0 34.0 30.0 34.0 2 31.747304209251674 31.0 31.0 34.0 30.0 34.0 3 31.78364257926994 31.0 31.0 34.0 30.0 34.0 4 35.572573876811 37.0 35.0 37.0 33.0 37.0 5 35.310925667272635 37.0 35.0 37.0 33.0 37.0 6 35.34686408737313 37.0 35.0 37.0 32.0 37.0 7 35.27788157500632 37.0 35.0 37.0 32.0 37.0 8 35.25097653700886 37.0 35.0 37.0 32.0 37.0 9 36.83520957331449 39.0 37.0 39.0 32.0 39.0 10-11 36.72205958280445 39.0 35.0 39.0 32.0 39.0 12-13 36.67576184044211 39.0 35.0 39.0 32.0 39.0 14-15 37.67384261174375 39.0 36.5 41.0 32.0 41.0 16-17 37.66041774414974 39.0 36.5 41.0 32.0 41.0 18-19 37.70229236781412 39.5 37.0 41.0 32.0 41.0 20-21 37.65870402069122 39.0 37.0 41.0 32.0 41.0 22-23 37.512603510268406 39.0 36.0 41.0 32.0 41.0 24-25 37.368129344865224 39.0 36.0 41.0 31.5 41.0 26-27 37.08945251545935 39.0 36.0 40.5 31.0 41.0 28-29 36.903195971477906 39.0 36.0 40.0 31.0 41.0 30-31 36.64069477140694 38.5 35.0 40.0 30.0 41.0 32-33 36.41779392226549 38.0 35.0 40.0 30.0 41.0 34-35 36.35494559752031 38.0 35.0 40.0 30.0 41.0 36-37 36.1881441394728 38.0 35.0 40.0 30.0 41.0 38-39 36.17324888078174 38.0 35.0 40.0 30.0 41.0 40-41 36.07681206291424 38.0 35.0 40.0 30.0 41.0 42-43 35.847677038671705 38.0 35.0 40.0 29.0 41.0 44-45 35.57827603888539 38.0 35.0 40.0 27.5 41.0 46-47 35.179798724319035 38.0 34.0 40.0 26.5 41.0 48-49 34.80590771155647 38.0 34.0 40.0 24.5 41.0 50-51 34.54086966101148 38.0 33.5 40.0 24.0 41.0 52-53 34.31192067541984 38.0 33.0 40.0 24.0 41.0 54-55 34.037069473158894 38.0 33.0 40.0 23.0 41.0 56-57 32.09323500641291 37.0 30.0 39.5 14.5 41.0 58-59 27.178733592209472 32.5 17.0 38.5 4.0 40.0 60-61 27.882474672419338 33.0 19.0 38.0 6.5 40.0 62-63 30.9214136017481 34.5 29.0 38.5 2.0 40.0 64-65 31.158823253548338 35.5 30.5 39.0 2.0 40.0 66-67 24.5771890068633 27.0 17.5 35.5 2.0 39.0 68-69 17.242275856607336 13.0 7.0 28.0 2.0 35.5 70-71 16.13095992846913 12.5 7.0 26.5 2.0 33.5 72-73 17.258767818012167 13.5 7.0 28.5 2.0 34.0 74-75 18.245062233357505 17.5 7.5 29.5 2.0 34.5 76-77 19.40958344596672 21.5 8.0 30.0 2.0 34.5 78-79 22.42485985162035 27.5 10.0 33.5 2.0 35.5 80-81 25.968194248242366 31.5 22.5 34.5 2.0 36.0 82-83 27.22755137299179 33.0 26.0 35.0 2.0 36.0 84-85 27.260651210490014 34.0 26.0 35.0 2.0 36.0 86-87 27.041836354120257 34.0 26.0 35.0 2.0 36.0 88-89 26.618661689708766 33.0 25.5 35.0 2.0 35.5 90-91 25.301054337001162 32.5 15.5 35.0 2.0 35.0 92-93 22.935021853458174 31.5 2.0 34.5 2.0 35.0 94-95 15.779923354730359 4.5 2.0 32.0 2.0 35.0 96-97 13.262432845798513 2.0 2.0 30.0 2.0 34.0 98-99 12.973914328427014 2.0 2.0 29.5 2.0 34.0 100-101 12.67544572958342 2.0 2.0 30.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.31840010934524443 1101 2 0.34302296312973724 1101 3 0.3919557575928181 1101 4 0.2604900893352351 1101 5 0.3512984930805487 1101 6 0.4526952386637717 1101 7 0.3820365692939731 1101 8 0.34260694781035994 1101 9 0.4301221752465807 1101 10-11 0.4973233605395322 1101 12-13 0.39174894011097905 1101 14-15 0.5822822890189983 1101 16-17 0.46888700615749457 1101 18-19 0.43124989062219043 1101 20-21 0.3636275707505021 1101 22-23 0.5044730722617032 1101 24-25 0.48896188793144546 1101 26-27 0.4443097213305691 1101 28-29 0.528711016739436 1101 30-31 0.4459694584624785 1101 32-33 0.5685155982830921 1101 34-35 0.41648518368720744 1101 36-37 0.12553038210377565 1101 38-39 0.42259905923102536 1101 40-41 0.5217432571066993 1101 42-43 0.4971948628065661 1101 44-45 0.5389226101839455 1101 46-47 0.5642359310011713 1101 48-49 0.6473154277788709 1101 50-51 0.6586497692055318 1101 52-53 0.7816450234992232 1101 54-55 0.5833848380767606 1101 56-57 -1.4968516079136087 1101 58-59 -9.317112612239164 1101 60-61 -9.607571738825815 1101 62-63 -3.406337154165225 1101 64-65 -4.513160849034481 1101 66-67 -9.429518344517984 1101 68-69 -4.982430321802198 1101 70-71 -4.814229060530472 1101 72-73 -4.754791825779655 1101 74-75 -6.4265727077688535 1101 76-77 -7.904133072969927 1101 78-79 -10.972264780065913 1101 80-81 -8.037193887545925 1101 82-83 -4.391863175931864 1101 84-85 -3.625179201753941 1101 86-87 -3.4604431664508972 1101 88-89 -3.5737654600598354 1101 90-91 -9.620151104650812 1101 92-93 -16.602685051932117 1101 94-95 -11.074992002992126 1101 96-97 -9.37522259232927 1101 98-99 -9.484178900778783 1101 100-101 -9.18767256359586 1102 1 0.3392009751461096 1102 2 0.3259883310951075 1102 3 0.3737739394109951 1102 4 0.2913126001577453 1102 5 0.27746732424937903 1102 6 0.24451342048195102 1102 7 0.3689196861770938 1102 8 0.324641579844986 1102 9 0.4443862445106532 1102 10-11 0.45982336053953077 1102 12-13 0.414725130587172 1102 14-15 0.35247709421380335 1102 16-17 0.32154934381982514 1102 18-19 0.5367260810983865 1102 20-21 0.574233631356563 1102 22-23 0.5199817302703593 1102 24-25 0.4208774723470299 1102 26-27 0.48331405033489716 1102 28-29 0.553981579509994 1102 30-31 0.4407530082460269 1102 32-33 0.46156754633503994 1102 34-35 0.3693531490551649 1102 36-37 0.4178896894630739 1102 38-39 0.4136380202699854 1102 40-41 0.31541208827552936 1102 42-43 0.4307987589104627 1102 44-45 0.5176347313960648 1102 46-47 0.5936190478842818 1102 48-49 0.4699885879520309 1102 50-51 0.34890950946526544 1102 52-53 0.28923160358581157 1102 54-55 0.20553851773043874 1102 56-57 -2.6349684910304916 1102 58-59 -10.1409762486028 1102 60-61 -10.305115028869103 1102 62-63 -4.260806851134923 1102 64-65 -4.90303097890461 1102 66-67 -6.158187175686816 1102 68-69 -4.021380538252416 1102 70-71 -3.967302653604065 1102 72-73 -4.307356760844591 1102 74-75 -5.191832448028594 1102 76-77 -7.266936103272958 1102 78-79 -9.66638815668929 1102 80-81 -5.348773974126011 1102 82-83 -2.731148890217579 1102 84-85 -2.368079634654375 1102 86-87 -2.2783219543296873 1102 88-89 -2.5016117804061544 1102 90-91 -8.602878377378087 1102 92-93 -12.855488082235146 1102 94-95 -10.37234048784061 1102 96-97 -9.217549432156108 1102 98-99 -9.313919160519042 1102 100-101 -9.033473429396725 1103 1 0.22896202035841995 1103 2 0.22315095787867278 1103 3 0.14470826982186225 1103 4 0.07481956250947519 1103 5 0.24826584582407918 1103 6 0.302383456583037 1103 7 0.23063276852194292 1103 8 0.2879903850710477 1103 9 0.3446673172285486 1103 10-11 0.36906781644463393 1103 12-13 0.42457299030867546 1103 14-15 0.5089314521302626 1103 16-17 0.4245869920921379 1103 18-19 0.4708428938884808 1103 20-21 0.3117078448686854 1103 22-23 0.4418138602342552 1103 24-25 0.4508271886956621 1103 26-27 0.4878039076496634 1103 28-29 0.4510634086315335 1103 30-31 0.5807517189216327 1103 32-33 0.5085092688724302 1103 34-35 0.5193595956771375 1103 36-37 0.563863559155358 1103 38-39 0.4845921203215582 1103 40-41 0.29398996346892403 1103 42-43 0.2877997903699736 1103 44-45 0.2002542087899073 1103 46-47 0.20042668068469283 1103 48-49 0.18371344612634743 1103 50-51 0.30392927910597933 1103 52-53 0.4692182805670697 1103 54-55 0.3566219799962127 1103 56-57 -2.3414069472794203 1103 58-59 -10.274066759175263 1103 60-61 -10.385688778224441 1103 62-63 -3.9854948346315666 1103 64-65 -4.987441757656544 1103 66-67 -6.198165686946915 1103 68-69 -3.986023825170072 1103 70-71 -3.90338095857226 1103 72-73 -4.158547666809865 1103 74-75 -4.766765832934901 1103 76-77 -6.3726237429497665 1103 78-79 -8.886192609156197 1103 80-81 -4.515062438970432 1103 82-83 -2.4383003428563974 1103 84-85 -1.9921306919003747 1103 86-87 -1.8902013595213667 1103 88-89 -1.9878456638512283 1103 90-91 -5.191593350732049 1103 92-93 -9.856041202400178 1103 94-95 -8.377239920537878 1103 96-97 -8.107466055845295 1103 98-99 -8.443356155533655 1103 100-101 -8.33693139742148 1104 1 0.28061170779527345 1104 2 0.34432009041848843 1104 3 0.24086558876355468 1104 4 0.24889869850188973 1104 5 0.3415656264173208 1104 6 0.33349975807560384 1104 7 0.34255229126848974 1104 8 0.2751095854694512 1104 9 0.30774266332321787 1104 10-11 0.3604828707076351 1104 12-13 0.4209087860678693 1104 14-15 0.4658863997606133 1104 16-17 0.47381151091672535 1104 18-19 0.4966316405015263 1104 20-21 0.4563670444397303 1104 22-23 0.6633907210151762 1104 24-25 0.5816606996970322 1104 26-27 0.6530491969276895 1104 28-29 0.5691057164278703 1104 30-31 0.49281747969349254 1104 32-33 0.6311712106301997 1104 34-35 0.6330612016900616 1104 36-37 0.7173106636703039 1104 38-39 0.6731614100578156 1104 40-41 0.4826144429944108 1104 42-43 0.6729334312015922 1104 44-45 0.7359377388044024 1104 46-47 0.6947302989073947 1104 48-49 0.6773061601990875 1104 50-51 0.7133862036246441 1104 52-53 0.8198931074998512 1104 54-55 0.6438007058141366 1104 56-57 -2.3866213064096193 1104 58-59 -10.055082525754472 1104 60-61 -10.139497072563618 1104 62-63 -3.9388818369688323 1104 64-65 -4.792404522945407 1104 66-67 -6.0649367402107295 1104 68-69 -3.798949532355124 1104 70-71 -3.907740753039519 1104 72-73 -3.984281879944678 1104 74-75 -4.646021413169521 1104 76-77 -6.4081514488206075 1104 78-79 -8.883659348109887 1104 80-81 -4.382947828771435 1104 82-83 -2.0215169986773596 1104 84-85 -1.4635839474416308 1104 86-87 -1.426896006251031 1104 88-89 -1.4914524905994426 1104 90-91 -3.604493521452465 1104 92-93 -6.1281991569691385 1104 94-95 -7.28351910648948 1104 96-97 -7.742100104783026 1104 98-99 -8.078771413451946 1104 100-101 -8.122517375754466 1105 1 0.33891001684449407 1105 2 0.34687812218028213 1105 3 0.2531277413225226 1105 4 0.19747347303265173 1105 5 0.10191458805721254 1105 6 0.19534823398106482 1105 7 0.13069673092039125 1105 8 0.10155522274442319 1105 9 0.2902071021609913 1105 10-11 0.353720848486752 1105 12-13 0.31898293317534154 1105 14-15 0.4875785912957653 1105 16-17 0.4083318863217258 1105 18-19 0.4396339724965088 1105 20-21 0.4399656790601938 1105 22-23 0.4589959398618362 1105 24-25 0.47237201687887165 1105 26-27 0.46252152728660434 1105 28-29 0.36083474706475727 1105 30-31 0.4664774436685093 1105 32-33 0.5338537681061624 1105 34-35 0.4239902969871636 1105 36-37 0.38443540544040644 1105 38-39 0.25093058560872805 1105 40-41 0.3336117837842849 1105 42-43 0.2806845746932609 1105 44-45 0.3334462851472253 1105 46-47 0.5799232853874585 1105 48-49 0.3740484712303882 1105 50-51 0.42743441854079833 1105 52-53 0.5516315866696289 1105 54-55 0.3674166366511926 1105 56-57 1.5381149057385208 1105 58-59 -8.548296725639087 1105 60-61 -9.616515688600138 1105 62-63 -3.5558093885619684 1105 64-65 -4.54806777159682 1105 66-67 -5.908677322011776 1105 68-69 -3.8096022247955936 1105 70-71 -4.120044766434987 1105 72-73 -2.4706917857959567 1105 74-75 -4.345573038403288 1105 76-77 -6.187095115368027 1105 78-79 -8.917012786647845 1105 80-81 -4.4332554931990025 1105 82-83 -1.9265202003757445 1105 84-85 -1.4268946562225047 1105 86-87 -1.264994964563975 1105 88-89 -1.4502364949456101 1105 90-91 -3.8493720761009165 1105 92-93 -6.248932639454125 1105 94-95 -7.97672389306771 1105 96-97 -8.005325800252193 1105 98-99 -8.469951047518958 1105 100-101 -8.563210805697157 1106 1 0.197742641812777 1106 2 0.19700023585701132 1106 3 0.18508346322052205 1106 4 0.12354474301488949 1106 5 0.018032800439854668 1106 6 0.16665627762480995 1106 7 0.0954970671294717 1106 8 0.17002848460689535 1106 9 0.2502791016535113 1106 10-11 0.2848233605395265 1106 12-13 0.238698344872887 1106 14-15 0.25399495135666683 1106 16-17 0.4494064866769705 1106 18-19 0.32328858109838876 1106 20-21 0.3090550599279922 1106 22-23 0.16337458741321598 1106 24-25 0.18699354377559985 1106 26-27 0.33521881223965977 1106 28-29 0.43560360331952097 1106 30-31 0.38369943681745866 1106 32-33 0.15429076062075353 1106 34-35 0.23542457762659552 1106 36-37 0.4101962370821255 1106 38-39 0.3117630202699928 1106 40-41 0.27188530256124466 1106 42-43 0.22410233033903637 1106 44-45 0.19443532663415652 1106 46-47 0.232235119312854 1106 48-49 0.18159573080917113 1106 50-51 0.05166248565574705 1106 52-53 0.19558577025247814 1106 54-55 0.075836136778058 1106 56-57 1.3928737708742709 1106 58-59 -8.50905660574566 1106 60-61 -9.739132886011962 1106 62-63 -3.7381282797063484 1106 64-65 -5.353745264618897 1106 66-67 -6.789109794734436 1106 68-69 -4.207213871585749 1106 70-71 -4.211171701223112 1106 72-73 -4.403398427511258 1106 74-75 -4.8858800670762115 1106 76-77 -6.57602836517772 1106 78-79 -8.89762327573691 1106 80-81 -4.594160878887912 1106 82-83 -2.3504941283128176 1106 84-85 -1.852945706082945 1106 86-87 -1.6730243352820686 1106 88-89 -1.6206742804061562 1106 90-91 -3.9498724249971353 1106 92-93 -5.9986130822351456 1106 94-95 -7.100465487840612 1106 96-97 -6.991239908346584 1106 98-99 -6.3591721367095175 1106 100-101 -6.828027000825298 1107 1 0.024684209860115658 1107 2 -0.048767938276547795 1107 3 0.03355134149608929 1107 4 -3.144041706804046E-5 1107 5 -0.10159156557873672 1107 6 -0.06286612305143535 1107 7 0.006879674906315358 1107 8 0.01507550489429832 1107 9 -0.033897779157989305 1107 10-11 0.05295522867140079 1107 12-13 -0.07896464117952462 1107 14-15 -0.02387117290368934 1107 16-17 -0.07838303149716097 1107 18-19 0.0043338579369347485 1107 20-21 0.035286040485900116 1107 22-23 -0.07024227649210957 1107 24-25 0.11632971241465384 1107 26-27 0.005633013423093303 1107 28-29 -0.03269072254692418 1107 30-31 0.1019448932840632 1107 32-33 0.18753119806792284 1107 34-35 0.12546050323944513 1107 36-37 0.03429149278796473 1107 38-39 0.004305814014699649 1107 40-41 -0.0067138626965785875 1107 42-43 -0.0640659917235169 1107 44-45 0.002678405670508255 1107 46-47 0.2144559033365212 1107 48-49 0.031125527935124353 1107 50-51 0.1641955055204889 1107 52-53 0.032254990455342636 1107 54-55 0.07972983922945076 1107 56-57 1.128343709589398 1107 58-59 -8.845118260437118 1107 60-61 -10.171759153974765 1107 62-63 -3.703634408193249 1107 64-65 -5.1366911648724916 1107 66-67 -6.836676891099607 1107 68-69 -3.9673892731073046 1107 70-71 -3.8373181509272545 1107 72-73 -2.340832387781756 1107 74-75 -4.706526728107489 1107 76-77 -6.8549947113315675 1107 78-79 -7.5407908053227075 1107 80-81 -2.595209589792397 1107 82-83 -1.6203430294117176 1107 84-85 -1.3259367775115187 1107 86-87 -1.2010198974122446 1107 88-89 -1.0674528624010833 1107 90-91 -0.7974289550055857 1107 92-93 -3.258674895421958 1107 94-95 -6.077327808212548 1107 96-97 -6.751098319166534 1107 98-99 -6.033824767732341 1107 100-101 -6.7898428292276645 1108 1 -0.18718357784417705 1108 2 -0.13274374004660316 1108 3 -0.18746312708157475 1108 4 -0.0773134310607233 1108 5 -0.09955485313008694 1108 6 -0.19972006518485586 1108 7 -0.23513475820140428 1108 8 -0.12676898853691654 1108 9 -0.12531700197281737 1108 10-11 -0.2040721228719491 1108 12-13 -0.20993241585475886 1108 14-15 -0.2869768793157803 1108 16-17 -0.2732254202611202 1108 18-19 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1.707214086792046 2316 92-93 3.778593055074147 2316 94-95 10.504190949977236 2316 96-97 12.455518066688091 2316 98-99 12.21822831983232 2316 100-101 10.848919081005496 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 15.0 4 43.0 5 102.0 6 300.0 7 611.0 8 1401.0 9 2434.0 10 4176.0 11 6877.0 12 9980.0 13 13865.0 14 16869.0 15 22543.0 16 30082.0 17 40524.0 18 41783.0 19 38017.0 20 38418.0 21 38432.0 22 38264.0 23 32977.0 24 33454.0 25 39543.0 26 54960.0 27 81374.0 28 113541.0 29 153557.0 30 191573.0 31 211598.0 32 214283.0 33 196960.0 34 177545.0 35 160648.0 36 131878.0 37 85953.0 38 33421.0 39 2278.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.48944682745458 20.768519661714343 14.819048327091483 21.922985183739595 2 15.098249947015688 22.807810086531454 39.22020368098268 22.873736285470176 3 18.49245977707167 27.557804836396233 31.691190198727764 22.258545187804334 4 11.9412480649547 18.22864658982968 39.553542631608515 30.276562713607102 5 11.910030217370114 39.666412639899335 34.99490469565526 13.428652447075295 6 28.375561821240968 39.21734579254014 18.136799683933383 14.270292702285511 7 25.858045209383075 34.9620821817445 22.420599544393337 16.759273064479093 8 25.25207477783352 34.39215800785934 20.891656734101925 19.464110480205214 9 26.145796787393436 16.93256994809942 21.958931691297064 34.96270157321008 10-11 23.710681361581713 27.17849932950874 28.80143769607611 20.309381612833437 12-13 25.814378111059543 25.37651258714064 28.561865925638514 20.247243376161304 14-15 22.291825571126964 26.417154956845824 28.272178343453074 23.018841128574135 16-17 20.374793775823647 29.814452438035417 28.529325752571694 21.281428033569245 18-19 20.213482542084968 28.578004878602346 31.032259962655047 20.17625261665764 20-21 21.623951514036076 27.159165467333075 30.508657544210173 20.70822547442068 22-23 21.03253273824403 27.433252650007056 30.44045044036638 21.093764171382535 24-25 21.332974971048973 27.583675934313522 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21.48130414966779 50-51 20.750549432054232 28.087338658313943 29.619259752695783 21.542852156936043 52-53 21.299584796552967 28.630771816114887 28.78794822898117 21.281695158350974 54-55 20.77902125241384 27.701502330767795 29.276002002133634 22.243474414684734 56-57 20.597362658673482 28.080030281343394 29.50923140630775 21.813375653675372 58-59 20.497225345105072 27.400764391872695 30.46352067261094 21.638489590411293 60-61 21.243509377289296 28.00787489981291 29.616693364696573 21.131922358201223 62-63 21.169943794436342 27.874725326748624 29.39148136292353 21.563849515891505 64-65 20.53614338025062 28.344888803057678 29.487465124303426 21.63150269238827 66-67 20.828563036778114 27.765458972970087 29.06503089168536 22.340947098566446 68-69 21.03940218318927 27.40165156998372 29.47025594646667 22.088690300360344 70-71 21.0869125281102 27.566776644148188 29.336860798522924 22.00945002921869 72-73 20.4215867376501 27.192871323455957 30.203514297494692 22.18202764139925 74-75 20.36591064628359 28.26329519047025 29.24448881019584 22.12630535305032 76-77 18.748557635053018 28.12345320727017 30.16226479499186 22.96572436268495 78-79 19.546898357423387 28.022991964823106 29.730935877538116 22.69917380021539 80-81 20.63388113594439 28.253092109849714 28.73543579466421 22.377590959541692 82-83 20.696175580210838 27.93194640923515 28.719858512948075 22.652019497605934 84-85 20.4317119047835 27.87713737299419 28.46558203727369 23.225568684948616 86-87 20.474645049271583 27.95336946309935 28.50969471239938 23.06229077522969 88-89 20.563115451276555 27.752128329091608 28.27513817409992 23.409618045531918 90-91 19.92281803963675 27.520781762829234 29.089899931324332 23.466500266209685 92-93 19.17795230409978 28.022185193113415 29.71187760971576 23.087984893071045 94-95 18.681228189076286 27.701463672600834 29.421407576732488 24.195900561590395 96-97 18.53579480663602 27.904413355685097 28.891414878906925 24.66837695877196 98-99 18.62522270845781 27.8732979511719 28.8764728362965 24.62500650407379 100-101 18.741533350895445 27.425268008233356 29.01920818692039 24.813990453950804 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1182.0 1 892.0 2 622.0 3 660.5 4 713.0 5 841.5 6 1103.0 7 1578.0 8 2087.0 9 2384.0 10 2574.5 11 2836.5 12 3147.0 13 3443.0 14 3827.5 15 4139.5 16 4402.5 17 4755.0 18 5181.0 19 5729.5 20 6247.0 21 7067.0 22 8075.5 23 9204.5 24 10831.5 25 13083.0 26 16182.5 27 20059.0 28 24048.5 29 28258.0 30 33173.5 31 39071.0 32 46137.0 33 53841.0 34 61352.0 35 67938.0 36 72865.5 37 76310.5 38 77853.0 39 77970.5 40 78210.5 41 78588.5 42 79176.5 43 80264.0 44 81836.5 45 83937.5 46 86650.0 47 90308.0 48 93269.0 49 88838.0 50 78806.0 51 69973.5 52 62975.5 53 57805.5 54 53240.5 55 48154.0 56 43063.0 57 38174.0 58 33765.5 59 29350.0 60 24544.5 61 19985.5 62 15791.0 63 12140.5 64 9215.5 65 6779.5 66 4829.5 67 3374.5 68 2136.5 69 1294.0 70 841.5 71 587.0 72 415.5 73 298.5 74 224.5 75 150.5 76 87.0 77 46.0 78 30.0 79 19.5 80 12.5 81 6.5 82 2.5 83 1.5 84 1.5 85 2.5 86 2.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023713844682280934 2 0.007963604557482403 3 2.2121123770784453E-4 4 0.0 5 0.14613214362980212 6 0.0 7 0.0 8 0.0018139321492043254 9 0.0 10-11 0.0 12-13 0.0 14-15 6.636337131235337E-4 16-17 0.0 18-19 1.9909011393706008E-4 20-21 0.0 22-23 2.654534852494134E-4 24-25 2.2121123770784454E-5 26-27 2.43332361478629E-4 28-29 0.003583622050867082 30-31 0.0138035812329695 32-33 0.0027430193475772723 34-35 0.0046675571156355195 36-37 0.10965441053177855 38-39 0.1085925965907809 40-41 0.12131224275898195 42-43 0.12412162547787157 44-45 0.11974164297125627 46-47 0.11281773123100072 48-49 0.11219833976541876 50-51 0.11047289211129757 52-53 0.08815267822657605 54-55 0.07488000396410538 56-57 0.5324112069152402 58-59 1.5188584792258315 60-61 0.5028352644337014 62-63 0.004313619135302969 64-65 0.11600317305399369 66-67 1.8639037678025276 68-69 2.7970833740730696 70-71 2.7141734021801693 72-73 2.5695454949667806 74-75 1.9625639798202261 76-77 1.3658245449795448 78-79 0.9127618090301082 80-81 0.04890980465720443 82-83 2.43332361478629E-4 84-85 1.1060561885392227E-4 86-87 1.9909011393706008E-4 88-89 2.2121123770784454E-5 90-91 0.24251387989910997 92-93 0.38052757110503416 94-95 1.9500655448897328 96-97 2.3646375254780043 98-99 1.3675721137574366 100-101 0.9119654485743599 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2260283.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.28682405806915 #Duplication Level Percentage of deduplicated Percentage of total 1 68.992684117803 25.72518073994462 2 14.56848315215752 10.864249361748843 3 5.818861473645447 6.509005919582843 4 3.1088160577723176 4.636715095002263 5 1.8024246251228349 3.360334493744319 6 1.1775952638846379 2.6345272449649197 7 0.8158980110462128 2.129557191104609 8 0.5438067595492863 1.622146157192236 9 0.43175124942888465 1.4488769586875716 >10 2.341901663979267 16.026310349692377 >50 0.22864683731645244 5.970993280876766 >100 0.15578347822727204 10.912659246798999 >500 0.007746206735270768 1.9105089372226138 >1k 0.004886068863786177 3.5999062923631318 >5k 3.575172339355739E-4 0.9483891672785731 >10k+ 3.575172339355739E-4 1.7006395637954153 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14069 0.622444180662333 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 13730 0.6074460587457411 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10473 0.46334905850285124 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7871 0.3482307303996889 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 7528 0.33305563949293077 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5944 0.26297591938708564 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4913 0.21736216217172807 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3835 0.16966901932191678 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3805 0.16834175189566972 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3763 0.1664835774989238 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3522 0.1558211958414057 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3425 0.15152969782987352 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3150 0.13936307975594206 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2918 0.12909887832629807 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2726 0.12060436679831685 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2709 0.11985224859011018 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2497 0.11047289211129757 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 6.636337131235337E-5 0.0 14-15 0.0 0.0 0.0 2.2121123770784456E-4 0.0 16-17 0.0 0.0 0.0 6.193914655819646E-4 0.0 18-19 0.0 0.0 0.0 8.848449508313781E-4 0.0 20-21 0.0 0.0 0.0 0.0012830251787054985 0.0 22-23 0.0 0.0 0.0 0.0024996869860986434 0.0 24-25 0.0 0.0 0.0 0.0042251346402198305 0.0 26-27 0.0 0.0 0.0 0.00566300768532082 0.0 28-29 0.0 0.0 0.0 0.011591468855891055 0.0 30-31 0.0 0.0 0.0 0.024687174128195453 0.0 32-33 0.0 0.0 0.0 0.05353311952529838 0.0 34-35 0.0 0.0 0.0 0.09332902118893961 0.0 36-37 0.0 0.0 0.0 0.15172878794381056 0.0 38-39 0.0 0.0 0.0 0.23669602434739367 0.0 40-41 0.0 0.0 0.0 0.33644017142986077 0.0 42-43 0.0 0.0 0.0 0.4335519047836045 0.0 44-45 0.0 0.0 0.0 0.5468563007375625 0.0 46-47 0.0 0.0 0.0 0.6821269725959094 0.0 48-49 0.0 0.0 0.0 0.7998777144277951 0.0 50-51 0.0 6.636337131235337E-5 0.0 0.9207917769589029 0.0 52-53 0.0 1.3272674262470674E-4 0.0 1.0357552571956696 0.0 54-55 0.0 1.3272674262470674E-4 0.0 1.1598768826735415 0.0 56-57 0.0 1.3272674262470674E-4 0.0 1.2638019221486867 0.0 58-59 0.0 1.3272674262470674E-4 0.0 1.3346337604627385 0.0 60-61 0.0 1.3272674262470674E-4 2.2121123770784454E-5 1.3959977578028946 0.0 62-63 0.0 1.7696899016627564E-4 4.424224754156891E-5 1.4493539083380267 0.0 64-65 0.0 1.7696899016627564E-4 4.424224754156891E-5 1.4995024959263952 4.424224754156891E-5 66-67 2.2121123770784454E-5 1.7696899016627564E-4 4.424224754156891E-5 1.5422405070515506 4.424224754156891E-5 68-69 4.424224754156891E-5 1.7696899016627564E-4 4.424224754156891E-5 1.602542690450709 4.424224754156891E-5 70-71 4.424224754156891E-5 1.7696899016627564E-4 4.424224754156891E-5 1.701401992582345 4.424224754156891E-5 72-73 4.424224754156891E-5 1.7696899016627564E-4 4.424224754156891E-5 1.83746902489644 4.424224754156891E-5 74-75 4.424224754156891E-5 1.7696899016627564E-4 4.424224754156891E-5 2.0122259026856373 4.424224754156891E-5 76-77 4.424224754156891E-5 1.7696899016627564E-4 4.424224754156891E-5 2.2800021059309827 4.424224754156891E-5 78-79 4.424224754156891E-5 1.7696899016627564E-4 4.424224754156891E-5 2.701807693992301 4.424224754156891E-5 80-81 4.424224754156891E-5 1.7696899016627564E-4 4.424224754156891E-5 3.207408098897351 4.424224754156891E-5 82-83 4.424224754156891E-5 1.7696899016627564E-4 4.424224754156891E-5 3.697191900306289 4.424224754156891E-5 84-85 4.424224754156891E-5 1.990901139370601E-4 4.424224754156891E-5 4.079710372550694 4.424224754156891E-5 86-87 4.424224754156891E-5 2.2121123770784456E-4 4.424224754156891E-5 4.37400980319721 4.424224754156891E-5 88-89 4.424224754156891E-5 2.2121123770784456E-4 4.424224754156891E-5 4.645104175008174 4.424224754156891E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 11560 0.0 26.761036 1 TACATGG 11700 0.0 26.115389 2 ACATGGG 11340 0.0 25.614208 3 CATGGGA 8280 0.0 23.879639 4 GAGTACT 10360 0.0 22.710579 12-13 CGTACGA 65 2.51584E-4 22.437662 68-69 ATGGGAT 3285 0.0 22.356203 5 AGTACTT 10640 0.0 21.671116 12-13 CATGGGG 3035 0.0 21.09546 4 TGCACCG 545 0.0 20.731157 5 GTACTTT 11370 0.0 20.713867 14-15 CATTCCG 500 0.0 20.684097 9 GCACCGT 590 0.0 19.122856 6 ACTTTTT 12905 0.0 18.541449 16-17 ATGGGAG 3385 0.0 18.079794 5 TACTTTT 13160 0.0 17.557049 14-15 AGAGTAC 20465 0.0 17.434677 10-11 CGCGGAA 395 0.0 17.256584 82-83 GTATCAA 29185 0.0 17.18246 1 TGGGATA 1145 0.0 16.833403 6 >>END_MODULE