FastQCFastQC Report
Fri 27 May 2016
SRR939584_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939584_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2272282
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58270.25643824138025123No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT54740.24090319775450403No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39990.17599048005485235No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT30310.1333901337950131No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29090.12802108189036396No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT24680.10861327951372232No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG92650.033.9274441
TACATGG114000.027.3080622
ACATGGG111150.026.691323
CATGGGA81800.024.6579044
GAGTACT99400.023.6495512-13
ATGGGAT32250.022.6840025
GAATAAC10250.022.1110761
AGTACTT105100.021.71569312-13
GTACTTT111000.021.20166414-15
CATGGGG32300.020.912944
CGTACAC1405.0359505E-420.246183
TAGGACG3204.274625E-1019.1899994
CCCTATA3759.094947E-1218.8960192
GTGCTAG6405.2568794E-918.4759671
CTGGTCG6650.018.4547279
ATGGGAG33250.018.3111555
ACTTTTT137350.018.05003716-17
GTATCAA301450.017.880431
TACTTTT136050.016.7080414-15
AGAGTAC207400.016.69349910-11
Produced by FastQC (version 0.11.5)