FastQCFastQC Report
Fri 27 May 2016
SRR939584_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939584_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2272282
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139520.6140082965054514No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT138190.6081551497569404No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104760.46103432584511955No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76960.33869035621458954No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT72520.31915052797144017No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT59690.2626874657282855No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC50160.22074724880098512No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT37780.1662645745554469No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT37740.16608854006676987No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA37400.1645922469130152No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC35100.15447026381408646No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC34340.15112560852922305No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA29510.12986944402147269No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC28190.12406030589513098No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA26930.1185152195018048No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG26100.11486250386175659No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25740.1132781934636634No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG113200.028.7724571
TACATGG115900.027.6049292
ACATGGG113400.026.7535213
CATGGGA83800.024.3233134
ATGGGAG33950.022.931225
CATGGGG30300.022.8906294
TCGCGCG1153.3960532E-922.85095460-61
GATTGGC7400.022.3004389
GTAGGAC7850.022.238993
AGTACTT109050.022.22360612-13
GAGTACT107100.022.0779412-13
TAGGACG3401.8189894E-1220.8159074
GTACTTT117100.020.23281114-15
ATGGGAT32450.019.1929725
CGCGGAA5100.018.4982-83
GGATATG10150.018.137311
CATTCCG3952.1827873E-1117.9048739
ACTTTTT135300.017.65057216-17
TACTTTT138350.017.46593314-15
CCCTATA5950.017.4480172
Produced by FastQC (version 0.11.5)