FastQCFastQC Report
Fri 27 May 2016
SRR939583_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939583_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2837511
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT142000.5004385886081146No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136680.481689762612374No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95970.3382189531600054No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT74000.26079194054225696No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67940.23943519514109374No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT61810.21783175466103918No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA60040.2115938933805014No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC53340.18798165011518897No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA47320.1667658733305351No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT43790.1543253929235869No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT36260.12778805086570588No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC33660.11862509079259957No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC31680.11164714427538783No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG133300.029.9446891
TACATGG135500.028.8630282
ACATGGG138800.026.8773863
CATGGGA94750.025.0476934
ATGGGAT40800.022.336655
GAGTACT104200.021.56401412-13
AGTACTT110250.020.27307512-13
GTACTTT112200.020.04726614-15
CATGGGG45650.019.8595564
ATGGGAG39300.018.9619985
GTATAGG8500.017.3099841
TTTGCGT7250.017.0211663
ACTTTTT142850.016.74337416-17
TAGGACA15000.016.4546644
TACTTTT136800.016.39024414-15
AGTACAT97550.016.056122
GATATAG15650.015.7704091
CTTTGCG7600.015.6127852
GTACAGG12950.015.3933621
CCCTATA6300.015.0675762
Produced by FastQC (version 0.11.5)