FastQCFastQC Report
Fri 27 May 2016
SRR939583_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939583_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2837511
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT161770.57011232731785No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143980.5074165351253264No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110890.3908002471179848No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT82660.2913116460165265No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82040.28912663246063186No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT68500.24140875577222431No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA61780.2177260281986572No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC58410.2058494222577463No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA52910.1864662374877137No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT45140.15908308373077673No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT43970.15495975169787887No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC39570.1394532038818528No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC38080.13420212291688033No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT32480.1144665166055744No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC31680.11164714427538783No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA30680.10812292886265462No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG30540.10762953870487198No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG130850.026.7257481
TACATGG135300.025.3195762
ACATGGG133100.024.7106173
CATGGGA89250.022.0897244
CATGGGG44600.021.7328594
GAGTACT109400.020.63597112-13
AGTACTT114150.019.6332112-13
ATGGGAT38700.019.5748925
ATGGGAG36000.019.4745885
GTACTTT118000.019.23153714-15
GTATAGG9300.018.7255081
GCTTACG7150.017.80644460-61
ATATAGG14700.017.6063042
CTCGCGA1253.6183537E-617.52278364-65
AGTACAT100500.017.1371212
ACTTTTT140850.016.51219716-17
CTTTGCG8700.016.22652
TACTTTT140700.015.861649514-15
TTACGCT4500.015.66524362-63
GAATAGG9950.015.6100991
Produced by FastQC (version 0.11.5)