FastQCFastQC Report
Fri 27 May 2016
SRR939582_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939582_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2649415
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT56760.214235972846836No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53490.20189362557394747No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37980.14335240043556785No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT31190.11772410135822436No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG103150.031.7164781
GTATAGG6500.028.6699051
TACATGG124600.026.7256552
GTCTACG2000.001001902325.8825551
ACATGGG124500.025.836583
CATGGGA84700.023.9206854
GAGTACT91300.022.715512-13
GTACTTT100850.020.66213814-15
ATGGGAT37750.020.4993725
AGTACTT101050.020.08012412-13
CATGGGG41800.019.9984684
CTTTGCG5750.018.0722452
AGTACAT89850.017.9789922
GTGTAGC11200.017.5631621
TTGCGTT6200.017.5200734
ACTTTTT128550.017.51639616-17
ATGGGAG35300.017.5109735
TAGGACA14450.017.322394
GTCCTAC6056.5125823E-717.112431
TACTTTT124650.016.39517414-15
Produced by FastQC (version 0.11.5)