##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939582_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2649415 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 14.902727205817133 2.0 2.0 31.0 2.0 33.0 2 22.387725969695197 16.0 16.0 31.0 16.0 34.0 3 26.99839209787821 28.0 25.0 31.0 16.0 34.0 4 32.269975824851905 35.0 32.0 35.0 25.0 37.0 5 32.90685717413089 35.0 33.0 35.0 26.0 37.0 6 33.32958558776183 35.0 33.0 37.0 28.0 37.0 7 33.508757216215656 35.0 33.0 37.0 28.0 37.0 8 33.444424146462524 35.0 33.0 37.0 27.0 37.0 9 34.80215443786647 37.0 34.0 39.0 27.0 39.0 10-11 34.64145405683897 37.0 34.0 39.0 27.0 39.0 12-13 34.651832763081664 37.0 34.0 39.0 27.0 39.0 14-15 35.301733778966295 38.0 33.5 40.0 27.0 41.0 16-17 34.949792123921696 38.0 33.0 40.0 25.0 41.0 18-19 35.25414685883487 38.0 33.5 40.0 27.0 41.0 20-21 35.265608445637994 38.0 34.0 40.0 26.5 41.0 22-23 35.08389172704163 38.0 34.0 40.0 25.5 41.0 24-25 34.891730249885356 38.0 33.0 40.0 25.0 41.0 26-27 34.961803454724915 38.0 34.0 40.0 25.0 41.0 28-29 34.71746008081029 38.0 33.0 40.0 24.0 41.0 30-31 34.59225583760943 38.0 33.0 40.0 24.0 41.0 32-33 34.27396123295142 38.0 33.0 40.0 24.0 41.0 34-35 33.990825521860486 38.0 33.0 40.0 23.0 41.0 36-37 33.708051400026044 38.0 32.0 40.0 20.5 41.0 38-39 33.49696083852473 37.5 32.0 40.0 19.0 41.0 40-41 33.138019902506784 37.0 31.0 40.0 17.0 41.0 42-43 32.87933506075869 37.0 31.0 40.0 16.0 41.0 44-45 32.517119439574394 37.0 30.5 40.0 15.0 41.0 46-47 32.148499763155264 37.0 30.0 39.5 11.0 41.0 48-49 31.75239232056888 36.5 30.0 39.0 9.0 40.0 50-51 31.087043932339782 35.5 29.5 38.5 8.0 40.0 52-53 31.143111592559112 36.0 29.5 39.0 8.0 40.0 54-55 31.27465459356122 36.0 29.5 39.0 7.0 40.0 56-57 30.98328668781599 36.0 29.0 39.0 2.0 40.0 58-59 30.579943685681556 35.0 28.0 39.0 2.0 40.0 60-61 29.981031472985546 35.0 28.0 38.0 2.0 40.0 62-63 29.602278427501922 34.0 27.0 38.0 2.0 40.0 64-65 29.032936138732513 34.0 26.0 38.0 2.0 40.0 66-67 28.425583194780735 34.0 26.0 37.0 2.0 39.0 68-69 28.071669972427877 33.5 25.5 37.0 2.0 39.0 70-71 27.535990775322098 33.0 24.5 36.0 2.0 39.0 72-73 26.932118033603643 32.0 22.5 36.0 2.0 38.5 74-75 26.37538135777143 32.0 22.0 35.0 2.0 38.0 76-77 25.73805972261801 31.5 20.5 35.0 2.0 37.0 78-79 25.06341701847389 31.0 18.5 34.5 2.0 36.5 80-81 24.30613286329246 30.5 14.0 34.0 2.0 36.0 82-83 23.60568616090722 30.0 7.5 34.0 2.0 35.5 84-85 22.908277487671807 29.0 4.5 34.0 2.0 35.0 86-87 22.237649065925872 29.0 2.0 34.0 2.0 35.0 88-89 21.421866713972705 29.0 2.0 33.5 2.0 35.0 90-91 20.475303604758032 27.0 2.0 33.0 2.0 34.0 92-93 19.4590081206606 25.5 2.0 32.0 2.0 34.0 94-95 18.46604250372252 25.0 2.0 32.0 2.0 34.0 96-97 17.472854384835898 22.0 2.0 31.5 2.0 34.0 98-99 16.396425248592614 17.0 2.0 31.0 2.0 34.0 100-101 14.789342741699581 2.0 2.0 30.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 14.939823555750696 1101 2 8.058899475015231 1101 3 3.8168716769800106 1101 4 2.4454883640643175 1101 5 1.6102389343130596 1101 6 1.4946928595297067 1101 7 1.165459181566078 1101 8 1.1202327535814973 1101 9 1.142043710513967 1101 10-11 1.1884846940936598 1101 12-13 0.9363284439031503 1101 14-15 0.9658695388257996 1101 16-17 1.088601929078017 1101 18-19 1.0341330557747597 1101 20-21 1.053103889373567 1101 22-23 0.9402617931707127 1101 24-25 1.1604189449909512 1101 26-27 1.205830311427654 1101 28-29 1.2111952708008502 1101 30-31 1.1947086884842193 1101 32-33 1.2405229414964936 1101 34-35 1.2407399628691849 1101 36-37 1.2022533691473072 1101 38-39 1.068609133202962 1101 40-41 1.248183815213764 1101 42-43 1.1951830666447663 1101 44-45 1.1815785071047316 1101 46-47 0.8920527119132515 1101 48-49 0.9261072752074817 1101 50-51 0.8872501469325123 1101 52-53 1.0759443533475377 1101 54-55 1.230469879057079 1101 56-57 0.8106096076535678 1101 58-59 0.881965880242408 1101 60-61 1.0587916883519384 1101 62-63 0.8559561717191606 1101 64-65 0.9835767872946697 1101 66-67 0.7461635919608867 1101 68-69 0.5550435152693503 1101 70-71 0.41953826661875837 1101 72-73 0.467719277113666 1101 74-75 0.4681226208643565 1101 76-77 0.3402685913950698 1101 78-79 0.39622111150469763 1101 80-81 0.21508982232787588 1101 82-83 0.20651639129000543 1101 84-85 0.02292043554776413 1101 86-87 -0.2723165102352638 1101 88-89 -0.15227604963019914 1101 90-91 0.023375381297558562 1101 92-93 0.10493550495417381 1101 94-95 0.004859581486080344 1101 96-97 -0.5909458223095179 1101 98-99 -0.8551729333303619 1101 100-101 -0.9835568549095601 1102 1 14.81079137755739 1102 2 8.011758768461736 1102 3 3.7574821613861147 1102 4 2.1566992307659802 1102 5 1.625912061708597 1102 6 1.3542289547668602 1102 7 1.1065376313873116 1102 8 1.0605485930965983 1102 9 1.1831974309628137 1102 10-11 1.1523411747956587 1102 12-13 0.7252178555047379 1102 14-15 0.8625667924383009 1102 16-17 1.150571444473833 1102 18-19 1.0767711427123388 1102 20-21 0.9301862116705095 1102 22-23 1.0008137942799564 1102 24-25 0.8959083031107653 1102 26-27 1.038805036343227 1102 28-29 1.1271756707255847 1102 30-31 1.0987089261809402 1102 32-33 1.0618604312210635 1102 34-35 1.2651373026467496 1102 36-37 1.1915310187229196 1102 38-39 1.014602457886781 1102 40-41 1.0215053297380265 1102 42-43 0.9783380449054206 1102 44-45 0.8678634064302742 1102 46-47 0.8322992232187048 1102 48-49 0.6582589752342223 1102 50-51 0.5263250114949045 1102 52-53 0.7246360309911708 1102 54-55 0.7506703861434083 1102 56-57 0.6529151667855793 1102 58-59 0.5022527999900497 1102 60-61 0.4830407152809961 1102 62-63 0.20203640426578318 1102 64-65 0.23134466654473584 1102 66-67 0.3970638682833254 1102 68-69 0.1595954074342707 1102 70-71 0.23922178334171207 1102 72-73 0.24864832464310638 1102 74-75 0.29206734647320687 1102 76-77 0.09119957021034963 1102 78-79 -0.2662916399338613 1102 80-81 -0.25935778040167534 1102 82-83 -0.22390656030900047 1102 84-85 -0.5693516974831638 1102 86-87 -0.5421072380824228 1102 88-89 -0.5276177386634693 1102 90-91 -0.3067445069849839 1102 92-93 -0.2596541215648571 1102 94-95 -0.0632702405385075 1102 96-97 -0.6913801140228166 1102 98-99 -1.1212583159725593 1102 100-101 -0.9360162731468424 1103 1 14.8330683706373 1103 2 7.958754595019606 1103 3 3.873148733257068 1103 4 2.354520873843093 1103 5 1.5775601702910151 1103 6 1.1670876289393988 1103 7 0.9294203447809721 1103 8 0.9848991816807597 1103 9 0.9534648764127454 1103 10-11 0.855945964166871 1103 12-13 0.6396009176980115 1103 14-15 0.8595995650185131 1103 16-17 1.0270179906284014 1103 18-19 0.9051817831667748 1103 20-21 0.7121742965782971 1103 22-23 0.8533219916786692 1103 24-25 0.9298342311972831 1103 26-27 0.9537173746132268 1103 28-29 1.1354557118523374 1103 30-31 0.9087881413696408 1103 32-33 1.0486346059514418 1103 34-35 0.9725175136765927 1103 36-37 1.07740123123547 1103 38-39 1.0681717596312623 1103 40-41 0.8221074040627272 1103 42-43 0.8089940069482466 1103 44-45 0.85625457730319 1103 46-47 0.7587979070166497 1103 48-49 0.6324301811572504 1103 50-51 0.7488251391045218 1103 52-53 0.9956515118606717 1103 54-55 0.7718408215474248 1103 56-57 0.8200193521379404 1103 58-59 0.8218717455214062 1103 60-61 0.8168632188115268 1103 62-63 0.6708595519210476 1103 64-65 0.5018563932608409 1103 66-67 0.5111523345615723 1103 68-69 0.2320108265650198 1103 70-71 0.30146914668396363 1103 72-73 0.18683908765138568 1103 74-75 0.03574094781103909 1103 76-77 0.14577030361320098 1103 78-79 0.04257103024268005 1103 80-81 0.06504956407866302 1103 82-83 -0.14851601411553617 1103 84-85 -0.22588859124633487 1103 86-87 -0.23778139361059658 1103 88-89 -0.3886174445536845 1103 90-91 -0.18663165147066962 1103 92-93 -0.052149946907398714 1103 94-95 -0.2701552047227409 1103 96-97 -0.9493399157782427 1103 98-99 -1.3042742051928293 1103 100-101 -1.14587028774538 1104 1 14.725798509104653 1104 2 7.975560924764753 1104 3 3.7717666649742974 1104 4 2.2398378303736948 1104 5 1.4624940261376054 1104 6 1.0890192579923976 1104 7 0.8781459755527408 1104 8 1.0285122052336035 1104 9 0.9261718751979799 1104 10-11 0.8324353724516627 1104 12-13 0.5042830426609868 1104 14-15 0.7199284704828273 1104 16-17 0.8562121999630392 1104 18-19 0.6953843923262539 1104 20-21 0.7051211691640873 1104 22-23 0.6732519781081479 1104 24-25 0.8227108296907275 1104 26-27 0.816792948320689 1104 28-29 0.8406766026298982 1104 30-31 0.7764196739961591 1104 32-33 0.8112451148006699 1104 34-35 0.8232371718185192 1104 36-37 0.9178514864299601 1104 38-39 0.6679709908246778 1104 40-41 0.793177457379521 1104 42-43 0.6524536232263216 1104 44-45 0.4142203494113943 1104 46-47 0.5772108548177712 1104 48-49 0.5436737353295946 1104 50-51 0.6003610989654575 1104 52-53 0.7071067233158779 1104 54-55 0.8109032302186598 1104 56-57 0.7196979082859052 1104 58-59 0.6337157023001687 1104 60-61 0.4694632099270635 1104 62-63 0.3899530628901857 1104 64-65 0.4018402275475843 1104 66-67 0.38805126311546445 1104 68-69 0.38783043323718047 1104 70-71 0.2559800319872281 1104 72-73 0.1983124020334266 1104 74-75 0.15220205273753962 1104 76-77 -0.050539404732617754 1104 78-79 -0.04519392963863211 1104 80-81 -0.03438009352551674 1104 82-83 -0.13809914913498034 1104 84-85 -0.07548666953047345 1104 86-87 -0.050998100006836466 1104 88-89 -0.12255067948992604 1104 90-91 0.041028351515315364 1104 92-93 0.334267213331227 1104 94-95 0.2957207448041643 1104 96-97 -0.29583060329199995 1104 98-99 -0.3804365917865091 1104 100-101 -0.5850514676487268 1105 1 14.785094944606811 1105 2 7.951049550969454 1105 3 3.8754191078100497 1105 4 2.345066560040955 1105 5 1.6124161657448326 1105 6 1.1140801744373832 1105 7 0.9904672010044564 1105 8 1.0765360321491357 1105 9 1.0577626393382218 1105 10-11 0.8871566013557626 1105 12-13 0.7240124300986537 1105 14-15 0.9261864541257836 1105 16-17 1.1953567849862239 1105 18-19 0.9309337146591901 1105 20-21 1.0207777176137895 1105 22-23 0.9248580190570834 1105 24-25 1.0322895204732205 1105 26-27 1.0382560809250307 1105 28-29 1.1535327435163225 1105 30-31 1.012362329862306 1105 32-33 0.6318579383255951 1105 34-35 0.9251513268742642 1105 36-37 0.8996361747339989 1105 38-39 1.0737613919192697 1105 40-41 1.085319622098318 1105 42-43 0.9220197945664239 1105 44-45 1.0045268213634841 1105 46-47 0.7744091639662329 1105 48-49 0.6833031080492056 1105 50-51 0.8359146799984067 1105 52-53 1.0038684986782016 1105 54-55 0.9656758587927889 1105 56-57 1.1438665873302796 1105 58-59 1.0863868952267701 1105 60-61 1.0997597267420005 1105 62-63 0.7743812001098149 1105 64-65 0.8446651356959443 1105 66-67 0.9911392294601526 1105 68-69 0.8177736823686352 1105 70-71 0.8330186660052235 1105 72-73 0.6355393909712532 1105 74-75 0.5900116318371609 1105 76-77 0.5667373008369871 1105 78-79 0.49124362123864174 1105 80-81 0.7072667219208206 1105 82-83 0.8719848278866671 1105 84-85 0.6783595044080108 1105 86-87 0.601183762609999 1105 88-89 0.9229211640297628 1105 90-91 1.0777176767373575 1105 92-93 1.1314480447748316 1105 94-95 1.1780746112040887 1105 96-97 0.6872411209386655 1105 98-99 0.4269361605284061 1105 100-101 0.4987072814245792 1106 1 14.609248309998591 1106 2 7.806515564480954 1106 3 3.5761511359509335 1106 4 2.145188782097563 1106 5 1.520995092128402 1106 6 0.9657045074328678 1106 7 0.9377879342250139 1106 8 0.9523430051410386 1106 9 1.0179583762850584 1106 10-11 0.8186777815438759 1106 12-13 0.5806283510120451 1106 14-15 0.7931203922657772 1106 16-17 1.0033152620919452 1106 18-19 0.7582173317799672 1106 20-21 0.6481745322014305 1106 22-23 0.5644210286261782 1106 24-25 0.6462688079335379 1106 26-27 0.6314821307118876 1106 28-29 0.934540069387154 1106 30-31 0.6794825937576121 1106 32-33 0.7986989057243505 1106 34-35 0.8765060517069543 1106 36-37 0.8364189818541803 1106 38-39 0.9101600193432162 1106 40-41 1.0239262114694654 1106 42-43 0.7762189885715856 1106 44-45 0.8508924385848644 1106 46-47 0.8077948476831196 1106 48-49 0.9311827370232812 1106 50-51 0.8104520620615787 1106 52-53 0.6610548435307138 1106 54-55 0.8588842203837608 1106 56-57 0.9083440088547832 1106 58-59 0.8741050224718592 1106 60-61 0.9327552687111194 1106 62-63 0.8171441894638285 1106 64-65 0.8265119114370982 1106 66-67 0.7384464665523112 1106 68-69 0.8490789943702453 1106 70-71 0.803783653112287 1106 72-73 0.7193849328472126 1106 74-75 0.7260570309965111 1106 76-77 0.5381551442325296 1106 78-79 0.6943128434517511 1106 80-81 0.6073620681669638 1106 82-83 0.7578800695174337 1106 84-85 0.5935401801879188 1106 86-87 0.8059427373607093 1106 88-89 0.7167502194358555 1106 90-91 0.9250719232085665 1106 92-93 1.3183088709288633 1106 94-95 1.1511397042307223 1106 96-97 0.49916843794094845 1106 98-99 0.16075990365341752 1106 100-101 0.2777436368969983 1107 1 14.645670583239514 1107 2 7.912874549139559 1107 3 3.7013834614917975 1107 4 2.0456667649889866 1107 5 1.3387730315038695 1107 6 1.0017233635751381 1107 7 0.6864929018535264 1107 8 0.6761375729191528 1107 9 0.5692083921562627 1107 10-11 0.745843104064015 1107 12-13 0.5365206646177612 1107 14-15 0.54888250430146 1107 16-17 0.8318427954532126 1107 18-19 0.6910366690216563 1107 20-21 0.6077083921123929 1107 22-23 0.5485674869241635 1107 24-25 0.8063521577152031 1107 26-27 0.6344033552992059 1107 28-29 0.6997210761176973 1107 30-31 0.6568474894289906 1107 32-33 0.6629147702316089 1107 34-35 0.5329996241587054 1107 36-37 0.6196700735043095 1107 38-39 0.552977694437196 1107 40-41 0.8323915643468851 1107 42-43 0.49225704021127825 1107 44-45 0.465693286741903 1107 46-47 0.4390676372863851 1107 48-49 0.509183057910132 1107 50-51 0.6905241808035605 1107 52-53 0.5778137940229513 1107 54-55 0.4960573457639512 1107 56-57 0.5516543837729699 1107 58-59 0.6250685487182075 1107 60-61 0.508419810368121 1107 62-63 0.32274470380619746 1107 64-65 0.24393283533728294 1107 66-67 0.16185533526457263 1107 68-69 0.47755972211391295 1107 70-71 0.3163986616134835 1107 72-73 0.18081918163147748 1107 74-75 0.2570529691230625 1107 76-77 0.2260492838921806 1107 78-79 0.3226169102885592 1107 80-81 0.18821158724068354 1107 82-83 0.15255513695561618 1107 84-85 0.2820553166975728 1107 86-87 0.29276213693293585 1107 88-89 0.35240634647010793 1107 90-91 0.6911664763274601 1107 92-93 0.8029856582282058 1107 94-95 1.042924308356774 1107 96-97 0.6355178190794923 1107 98-99 0.5366922357736108 1107 100-101 0.6204596785845862 1108 1 14.318228882069285 1108 2 7.591546300899893 1108 3 3.354457028644177 1108 4 -0.13404366591713313 1108 5 0.39546921757021636 1108 6 0.5390679557866846 1108 7 0.47942888569554043 1108 8 0.5165367368952758 1108 9 0.4945692194706055 1108 10-11 0.5063364965041828 1108 12-13 0.2826340381646162 1108 14-15 0.35601496055819837 1108 16-17 0.5911270038785474 1108 18-19 0.3841832465063888 1108 20-21 0.40036165654066735 1108 22-23 -0.2838184585301562 1108 24-25 0.11764137185049606 1108 26-27 0.422073931046576 1108 28-29 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0.9227941053354094 1108 94-95 0.8972184567566828 1108 96-97 0.4373777227076694 1108 98-99 0.17146797112193113 1108 100-101 0.18417655998418958 1109 1 14.161979267893521 1109 2 6.357820756675697 1109 3 2.814006436704698 1109 4 -4.154598304340833 1109 5 -0.9353346087908179 1109 6 -0.12017426983329216 1109 7 -0.04027183354429553 1109 8 0.05003308854129074 1109 9 0.25204878650744433 1109 10-11 0.26490750622193815 1109 12-13 0.14965561120594373 1109 14-15 0.17792991572297012 1109 16-17 0.389758367757274 1109 18-19 0.35327086434937627 1109 20-21 0.2715537660297045 1109 22-23 0.21213464185822062 1109 24-25 0.43382783950743686 1109 26-27 0.26348554409361924 1109 28-29 0.3683127828389061 1109 30-31 0.29525658898916873 1109 32-33 0.1708601022776577 1109 34-35 0.2483028793900104 1109 36-37 0.11131935148452499 1109 38-39 0.4342205789174969 1109 40-41 0.43474656346447915 1109 42-43 0.1789885208276516 1109 44-45 -0.017287947318465058 1109 46-47 -0.27662111041675175 1109 48-49 -0.20956834789163636 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0.12851888292128422 2312 9 -0.01634103019613775 2312 10-11 0.01686497081077931 2312 12-13 -0.5343422395165192 2312 14-15 0.23598588006653642 2312 16-17 -0.1316709041199644 2312 18-19 5.919492089034861E-4 2312 20-21 0.18113284732123702 2312 22-23 0.411057317071986 2312 24-25 0.355995430592678 2312 26-27 0.5658103621709003 2312 28-29 -0.3531133399137403 2312 30-31 0.3113954473226741 2312 32-33 0.4329027541601107 2312 34-35 -0.49447274513522643 2312 36-37 0.32327864997534306 2312 38-39 0.490554828854485 2312 40-41 0.5144219049274099 2312 42-43 0.4659349594282318 2312 44-45 0.5428189423062264 2312 46-47 0.6519605064989591 2312 48-49 0.5898847464185089 2312 50-51 0.5695285992131858 2312 52-53 0.34741313313901756 2312 54-55 0.21429799262913107 2312 56-57 0.3611170400757757 2312 58-59 0.39548648270491427 2312 60-61 0.39393794086766576 2312 62-63 0.2488020555141084 2312 64-65 0.38840231326400954 2312 66-67 0.3396249771606108 2312 68-69 0.5897466471261161 2312 70-71 0.5544107527854223 2312 72-73 0.4546078740075359 2312 74-75 0.46552429952747687 2312 76-77 0.498728993003116 2312 78-79 0.8790523927463454 2312 80-81 0.7619251791698893 2312 82-83 0.508262770916037 2312 84-85 0.5525133042559354 2312 86-87 0.74631876892823 2312 88-89 0.9984347845059816 2312 90-91 1.343473311719798 2312 92-93 1.862236781605727 2312 94-95 2.0236247451910394 2312 96-97 1.8093747276947667 2312 98-99 1.4612075249729166 2312 100-101 1.5109394452725322 2313 1 -14.179918642349712 2313 2 -7.405354642802742 2313 3 -3.7939413260773307 2313 4 -1.1497261945769317 2313 5 -0.5694168517718055 2313 6 -1.0064917086756253 2313 7 -0.4666613817743297 2313 8 -0.29259043005730234 2313 9 -2.5357358107975543 2313 10-11 -0.7060534639959073 2313 12-13 -0.853392190772098 2313 14-15 -1.0590204788589048 2313 16-17 -0.7882225341179563 2313 18-19 -0.24262065461507376 2313 20-21 -1.2309318423068376 2313 22-23 -1.9935796767741394 2313 24-25 -1.2817775955483839 2313 26-27 -0.4480398135200758 2313 28-29 0.037483162781640544 2313 30-31 -1.259833273820064 2313 32-33 -0.5612042511420441 2313 34-35 -1.3187281315498325 2313 36-37 -1.4986535193216852 2313 38-39 -0.802725759687668 2313 40-41 -0.5158908479712139 2313 42-43 -0.38405087998820164 2313 44-45 -0.4874473624865985 2313 46-47 -0.904331002508961 2313 48-49 -0.5447854766692473 2313 50-51 -1.225179065338942 2313 52-53 -0.30141535401196506 2313 54-55 -1.0344428056009924 2313 56-57 -0.6425741056511249 2313 58-59 -0.3673712923832646 2313 60-61 -1.9185816154665254 2313 62-63 -0.7380150326570387 2313 64-65 -0.45315113638636006 2313 66-67 -1.4813517296652101 2313 68-69 -0.48011101407432477 2313 70-71 -0.33777693096293504 2313 72-73 -1.2339689421134032 2313 74-75 -0.7590906219004196 2313 76-77 -0.15839201770766564 2313 78-79 -0.10451901080755377 2313 80-81 -0.11701905364541076 2313 82-83 -0.03141011612425615 2313 84-85 0.026080098951592134 2313 86-87 -0.06385020011865805 2313 88-89 0.27350812148403136 2313 90-91 0.6203594585403422 2313 92-93 0.9661056478753061 2313 94-95 1.0996718339443845 2313 96-97 0.7950699868334468 2313 98-99 0.7730864917629283 2313 100-101 0.8475782678171075 2314 1 -14.179918642349712 2314 2 -7.3345286982636875 2314 3 -3.7398148797065467 2314 4 -1.8745833552611337 2314 5 -1.4923788996480596 2314 6 -6.484936023084252 2314 7 -2.077122661762033 2314 8 -4.379475182693604 2314 9 -1.9330900101421449 2314 10-11 -0.7420506338297272 2314 12-13 -3.078437800340705 2314 14-15 -3.1778539724350168 2314 16-17 -2.8860959224855023 2314 18-19 -1.5207166427316494 2314 20-21 -2.4541053697013666 2314 22-23 -1.5601789218222493 2314 24-25 -3.3911420897790414 2314 26-27 -4.969188548292699 2314 28-29 -2.1811871047904816 2314 30-31 -2.328627596046097 2314 32-33 -4.433140375823541 2314 34-35 -2.9096813685222926 2314 36-37 -1.4081447543105199 2314 38-39 -1.8614841700246672 2314 40-41 -2.96493888298356 2314 42-43 -1.8842811863269446 2314 44-45 -3.159135438086821 2314 46-47 -2.858949210730465 2314 48-49 -1.7960962473691744 2314 50-51 -2.8129104226310417 2314 52-53 -1.419662903453748 2314 54-55 -0.8989883492817441 2314 56-57 -1.714509182390593 2314 58-59 -2.1259552023055406 2314 60-61 -1.1755992736509668 2314 62-63 -3.5274691464076504 2314 64-65 -1.7885918971874482 2314 66-67 -2.8122674888582537 2314 68-69 -2.6322780377238466 2314 70-71 -3.361890016675197 2314 72-73 -3.3337304329181343 2314 74-75 -2.5946376825675905 2314 76-77 -1.3076636498207534 2314 78-79 -0.8662498785782304 2314 80-81 -0.7962377972086969 2314 82-83 -0.9185477841473073 2314 84-85 -0.47517984053758155 2314 86-87 -0.25258190841110917 2314 88-89 -0.05419961013584995 2314 90-91 0.2964438316048117 2314 92-93 0.7370203422628911 2314 94-95 0.7864548518873171 2314 96-97 0.5529865865482613 2314 98-99 0.41403451311589023 2314 100-101 0.36395772208262933 2315 1 -14.179918642349712 2315 2 -7.399536217980698 2315 3 -4.630226515336513 2315 4 -2.7618011537023186 2315 5 -3.27982519665769 2315 6 -2.7608739800038045 2315 7 -3.2012154094563705 2315 8 -3.4733108387811527 2315 9 -2.3193289723464616 2315 10-11 -4.52840029224723 2315 12-13 -4.609126897251382 2315 14-15 -3.493565614563117 2315 16-17 -4.360813953681344 2315 18-19 -1.6364676536468679 2315 20-21 -3.359588296656689 2315 22-23 -4.407917730946835 2315 24-25 -5.129657341827311 2315 26-27 -4.583859387611678 2315 28-29 -2.636851555041723 2315 30-31 -3.2513847183701614 2315 32-33 -4.423499899744954 2315 34-35 -3.966175514695255 2315 36-37 -5.159501286432118 2315 38-39 -3.7027928705899527 2315 40-41 -4.67491043434811 2315 42-43 -3.5635324801578143 2315 44-45 -2.2449379868991173 2315 46-47 -1.5399340358870646 2315 48-49 -1.2464692184927735 2315 50-51 -0.9289981202777433 2315 52-53 -0.7281753649795633 2315 54-55 -2.3360662479258636 2315 56-57 -4.454437936032811 2315 58-59 -3.928270689933189 2315 60-61 -4.690445663813122 2315 62-63 -2.62525447479565 2315 64-65 -3.219796968414176 2315 66-67 -1.8455573577670314 2315 68-69 -1.559126701851472 2315 70-71 -2.0605975728349364 2315 72-73 -2.235046196557967 2315 74-75 -2.9464027355235487 2315 76-77 -2.5262392698759832 2315 78-79 -1.832839737264802 2315 80-81 -1.483171071294624 2315 82-83 -1.1549400604168838 2315 84-85 -0.9183071434627941 2315 86-87 -1.6308053070242607 2315 88-89 -2.167670702134547 2315 90-91 -1.7225991724616492 2315 92-93 -0.7027723306586147 2315 94-95 -0.5819526338063454 2315 96-97 -1.2501953817365568 2315 98-99 -0.8645410765387318 2315 100-101 -0.4609518139420281 2316 1 -14.179918642349712 2316 2 -7.302678913580264 2316 3 -4.959960631479355 2316 4 -2.752747604174928 2316 5 -2.1230991947193587 2316 6 -1.1407328869249937 2316 7 -4.751046415758914 2316 8 -2.849236213697779 2316 9 -1.2457350705020076 2316 10-11 -4.2436825608729905 2316 12-13 -3.5583632753301657 2316 14-15 -1.8037969409739674 2316 16-17 -3.203812970001458 2316 18-19 -2.3039306765855443 2316 20-21 -3.2833071872030004 2316 22-23 -2.8335750844323044 2316 24-25 -3.4853648293399857 2316 26-27 -4.095002217799237 2316 28-29 -3.7647696569287916 2316 30-31 -2.6064152414754247 2316 32-33 -2.8884332621950364 2316 34-35 -3.996444420184794 2316 36-37 -2.324827922913247 2316 38-39 -3.298316170476408 2316 40-41 -2.695013196238861 2316 42-43 -6.459528025559159 2316 44-45 -3.3932071127435037 2316 46-47 -4.731208247706135 2316 48-49 -4.305537579296793 2316 50-51 -3.298127725215803 2316 52-53 -4.728603209651823 2316 54-55 -4.735655886497728 2316 56-57 -4.712048458174841 2316 58-59 -3.4680620257651995 2316 60-61 -3.09738657770518 2316 62-63 -2.1767111589037746 2316 64-65 -2.2119083307415437 2316 66-67 -2.433647837843722 2316 68-69 -2.4215806763866325 2316 70-71 -2.7336170346545003 2316 72-73 -3.4687711765512006 2316 74-75 -4.088030405759216 2316 76-77 -3.6806010094125945 2316 78-79 -3.847841482290491 2316 80-81 -3.9394483146422523 2316 82-83 -3.5998251559182783 2316 84-85 -3.122647408297656 2316 86-87 -3.3341480373264147 2316 88-89 -3.1611364550982906 2316 90-91 -2.1971884316801003 2316 92-93 -2.278860530656374 2316 94-95 -1.4395832781727478 2316 96-97 -1.730594553888455 2316 98-99 -1.9002173844998111 2316 100-101 -1.7001165898893067 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 17801.0 3 5389.0 4 5829.0 5 6787.0 6 8020.0 7 10058.0 8 12530.0 9 15441.0 10 18599.0 11 22514.0 12 26026.0 13 29289.0 14 31657.0 15 35618.0 16 39821.0 17 41782.0 18 37946.0 19 38050.0 20 40942.0 21 44535.0 22 49363.0 23 54896.0 24 61257.0 25 68408.0 26 76514.0 27 86038.0 28 97560.0 29 109596.0 30 124517.0 31 142443.0 32 164340.0 33 189979.0 34 221407.0 35 257787.0 36 272947.0 37 163077.0 38 20556.0 39 96.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.51594965158106 19.55399397083393 13.864020210863586 22.06603616672142 2 14.880818140710863 21.700999519109633 40.50725056503674 22.910931775142764 3 19.355472580509243 26.982958541154083 30.386375599844463 23.27519327849221 4 11.93223889966683 17.00619069769201 39.80183716612021 31.259733236520958 5 12.413821250043458 39.06839278615275 35.03289946051838 13.484886503285413 6 29.10037550188507 38.076359861895135 18.591198876896314 14.232065759323476 7 26.502076685589387 34.18798701556447 22.481846248527116 16.828090050319037 8 25.118119731202242 34.32932298937518 21.047633844142815 19.50492343527976 9 26.591670266811352 17.239495987303304 21.59179688607328 34.57703685981207 10-11 24.082644868595366 26.839930318632206 28.573347878314216 20.504076934458208 12-13 26.150455196676752 24.927122986539228 28.263316909980613 20.65910490680341 14-15 23.037264295097973 25.9396113887158 27.671596268221883 23.351528047964347 16-17 21.08381411128649 29.130967391764486 28.17954108383679 21.605677413112236 18-19 20.81406785580905 28.53435202223254 30.28037884824449 20.371201273713925 20-21 22.25248127945335 27.00719480768442 29.770135564567525 20.97018834829471 22-23 21.68326480785638 27.098857454906305 29.702504129681063 21.515373607556256 24-25 21.953697253660014 27.148537574571037 29.920108914705736 20.977656257063217 26-27 21.748750394092294 26.80009161286491 30.56384087125272 20.887317121790076 28-29 21.113453543154893 27.655950087347897 30.478392624686595 20.752203744810615 30-31 21.367250570865217 27.265264307947927 30.09344938782366 21.274035733363196 32-33 21.230913206142574 27.53476348385894 30.059696676652365 21.174626633346126 34-35 21.950882809134484 27.098872429418574 29.887844302748523 21.06240045869842 36-37 21.804931664952644 26.735235229841013 30.384851082142543 21.0749820230638 38-39 21.472128630689706 27.4198421611892 30.396563394555432 20.711465813565667 40-41 21.341650875739425 27.448060107750155 30.300504717236205 20.909784299274218 42-43 20.988905633509106 27.7730480685298 29.439065306098527 21.798980991862567 44-45 21.56591601384081 27.3552076717068 29.369420236936595 21.709456077515796 46-47 21.901908710333874 27.708539461048883 28.938941053793922 21.450610774823325 48-49 21.814597250058824 27.675642978253023 28.985073023323732 21.52468674836442 50-51 21.38148199854189 27.927334348032858 29.308055889437856 21.383127763987396 52-53 21.60414529654958 28.38975233465277 28.574696683887645 21.431405684910004 54-55 21.22868626027552 27.746822012453322 28.97959114172569 22.04490058554547 56-57 21.725009131230777 28.087684783293337 28.443060368642588 21.744245716833298 58-59 22.09862689953442 27.656011154964194 28.461872237846524 21.78348970765486 60-61 21.720868907365045 27.818339721579488 29.17982936566238 21.28096200539309 62-63 21.587021068431845 27.785131418168042 29.134522328740754 21.493325184659366 64-65 21.35968992248062 28.21652486292305 28.908848553601814 21.514936660994515 66-67 21.443727014894073 27.94168228709994 28.379974658120037 22.23461603988595 68-69 21.1206611320241 28.117566093350298 28.75597217679345 22.005800597832152 70-71 21.7803357637015 27.829203068927583 28.40633829529824 21.984122872072675 72-73 21.590224778943163 27.5109091019131 28.71972383782508 22.179142281318654 74-75 21.165551607563774 28.70845677245397 28.204811916902102 21.92117970308016 76-77 21.550195443566366 27.712783377090016 28.362861919654826 22.374159259688792 78-79 21.412708733487236 27.88480183549449 28.587565576684167 22.11492385433411 80-81 21.658809538134033 28.164031416405866 28.13754458773819 22.03961445772191 82-83 21.58708754979784 27.88720580281617 28.397825778930986 22.127880868455012 84-85 21.3108497788106 27.911337394019714 28.224399066828852 22.55341376034083 86-87 21.37511681670714 28.07735540403875 28.292840972860695 22.254686806393416 88-89 21.56837526250456 27.783390022150346 27.946732497946396 22.701502217398698 90-91 21.35684683243294 27.500195737795323 28.418253277520638 22.7247041522511 92-93 21.65085766146243 28.04310821362874 28.26024918827439 22.045784936634437 94-95 21.468903145933414 27.77070222754969 28.164341194383148 22.596053432133743 96-97 21.087582751190624 27.651643177206676 28.299610878741856 22.96116319286085 98-99 21.327305038302715 27.930820601566403 27.582023910403088 23.15985044972779 100-101 21.365742572083654 27.605188516581958 27.956545586895388 23.072523324439 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4360.0 1 3015.0 2 1545.0 3 1275.5 4 1129.5 5 1185.5 6 1637.5 7 3410.0 8 4603.0 9 4088.0 10 3470.5 11 3150.5 12 3253.5 13 3472.5 14 3703.5 15 3944.0 16 4123.0 17 4426.0 18 4824.0 19 5222.0 20 5854.5 21 6843.5 22 8079.0 23 9546.0 24 11266.0 25 14150.5 26 18425.0 27 22470.5 28 25676.5 29 30381.5 30 36466.0 31 43247.0 32 50565.5 33 58054.0 34 64873.0 35 71153.0 36 79718.5 37 85577.5 38 86888.5 39 86926.0 40 86653.0 41 86340.5 42 85899.5 43 87619.5 44 90239.5 45 92112.5 46 97318.5 47 116625.0 48 129634.5 49 115033.5 50 94292.5 51 82034.0 52 76185.5 53 72543.0 54 68020.5 55 62536.0 56 56528.0 57 50635.5 58 45204.5 59 39592.0 60 32871.5 61 26024.0 62 20523.0 63 15658.0 64 11610.5 65 8422.5 66 6004.5 67 4271.0 68 2911.5 69 2053.0 70 1384.0 71 897.5 72 613.5 73 406.0 74 272.5 75 183.5 76 126.0 77 79.5 78 53.0 79 38.5 80 29.0 81 19.5 82 15.0 83 14.5 84 8.5 85 5.0 86 4.5 87 5.5 88 6.0 89 5.5 90 4.0 91 4.0 92 3.5 93 1.5 94 3.0 95 4.5 96 3.5 97 2.5 98 1.5 99 1.5 100 1.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 54.38770445551187 2 0.08462245439087497 3 0.11715793863928453 4 0.1934389289711125 5 0.11968679878388247 6 0.14678712093046956 7 0.15452467808931405 8 0.15120319013820033 9 0.13920054049667568 10-11 0.12721676294578238 12-13 0.13929490094983232 14-15 0.1459378768520598 16-17 0.13976670321561552 18-19 0.14807042309339988 20-21 0.17590675677460874 22-23 0.1941183242338403 24-25 0.22187916955252385 26-27 0.2144435658437806 28-29 0.2142548449374673 30-31 0.22869199427043327 32-33 0.21893512341403668 34-35 0.2042715089934948 36-37 0.19077796419209525 38-39 0.1866449763438344 40-41 0.18736211578782486 42-43 0.18058703525117809 44-45 0.1964773355627563 46-47 0.22376637861565665 48-49 0.22695576193235112 50-51 0.23658052815432842 52-53 0.2641526525666987 54-55 0.2515838402062342 56-57 0.2270123782042451 58-59 0.1831536395770387 60-61 0.19270291743649068 62-63 0.19710011455359014 64-65 0.1855126509059547 66-67 0.19534501012487662 68-69 0.18343672093650862 70-71 0.197553044728742 72-73 0.2170667864415352 74-75 0.24837558479890845 76-77 0.24837558479890845 78-79 0.2423742599781461 80-81 0.24880964288342897 82-83 0.2398076556522855 84-85 0.20861208983870022 86-87 0.22082233247716948 88-89 0.22229435554641305 90-91 0.21031057799551978 92-93 0.20798931084786643 94-95 0.24575236420115382 96-97 0.2758344766674907 98-99 0.29295146287010526 100-101 0.2890449401094204 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2649415.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.68633441544233 #Duplication Level Percentage of deduplicated Percentage of total 1 81.29803550485593 53.401699474904696 2 10.965354945475761 14.405479438650904 3 3.585616484790071 7.065780105163301 4 1.5151327927960194 3.9809407724560963 5 0.7706092298473539 2.5309247787689877 6 0.4737739876431245 1.8672285953798347 7 0.2934811510301039 1.3494390721834637 8 0.20463658765466214 1.0753461864255291 9 0.14540289224867334 0.8595884704696996 >10 0.6932005722161922 7.830324566468228 >50 0.03674647940563573 1.6163153026940047 >100 0.01571090619586745 1.7430744152871158 >500 0.0012168348567740195 0.5766463696649247 >1k 9.464271108241062E-4 1.207644745834411 >5k 1.3520387297487232E-4 0.489567705648786 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5676 0.214235972846836 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5349 0.20189362557394747 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3798 0.14335240043556785 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3119 0.11772410135822436 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 3.774418126265609E-5 0.0 0.0 0.0 5 3.774418126265609E-5 3.774418126265609E-5 0.0 0.0 0.0 6 7.548836252531218E-5 3.774418126265609E-5 0.0 0.0 0.0 7 7.548836252531218E-5 3.774418126265609E-5 0.0 0.0 0.0 8 7.548836252531218E-5 3.774418126265609E-5 0.0 0.0 0.0 9 7.548836252531218E-5 3.774418126265609E-5 0.0 0.0 0.0 10-11 7.548836252531218E-5 3.774418126265609E-5 0.0 3.774418126265609E-5 0.0 12-13 7.548836252531218E-5 3.774418126265609E-5 0.0 7.548836252531218E-5 0.0 14-15 7.548836252531218E-5 3.774418126265609E-5 0.0 1.1323254378796828E-4 0.0 16-17 7.548836252531218E-5 3.774418126265609E-5 0.0 3.019534501012488E-4 0.0 18-19 9.436045315664023E-5 3.774418126265609E-5 0.0 4.15185993889217E-4 0.0 20-21 1.1323254378796828E-4 3.774418126265609E-5 0.0 5.284185376771853E-4 0.0 22-23 1.5097672505062437E-4 3.774418126265609E-5 0.0 0.0010568370753543707 0.0 24-25 1.5097672505062437E-4 3.774418126265609E-5 0.0 0.0021514183319713974 0.0 26-27 1.5097672505062437E-4 3.774418126265609E-5 0.0 0.0028874298665931917 0.0 28-29 2.075929969446085E-4 3.774418126265609E-5 0.0 0.006076813183287632 0.0 30-31 2.2646508757593657E-4 3.774418126265609E-5 0.0 0.014418277242334628 3.774418126265609E-5 32-33 2.2646508757593657E-4 3.774418126265609E-5 0.0 0.03044068218833214 3.774418126265609E-5 34-35 2.4533717820726463E-4 3.774418126265609E-5 0.0 0.053766586208653606 3.774418126265609E-5 36-37 3.0195345010124874E-4 3.774418126265609E-5 0.0 0.08890641896418644 3.774418126265609E-5 38-39 3.3969763136390487E-4 3.774418126265609E-5 0.0 0.14091790074412652 3.774418126265609E-5 40-41 3.3969763136390487E-4 3.774418126265609E-5 0.0 0.19961010260755677 3.774418126265609E-5 42-43 3.3969763136390487E-4 3.774418126265609E-5 0.0 0.26339776894144556 3.774418126265609E-5 44-45 3.3969763136390487E-4 3.774418126265609E-5 0.0 0.3371687712193069 3.774418126265609E-5 46-47 4.1518599388921706E-4 3.774418126265609E-5 0.0 0.4297552478566023 3.774418126265609E-5 48-49 4.1518599388921706E-4 3.774418126265609E-5 0.0 0.5260783984389007 3.774418126265609E-5 50-51 4.1518599388921706E-4 3.774418126265609E-5 0.0 0.6334605941311573 3.774418126265609E-5 52-53 4.1518599388921706E-4 3.774418126265609E-5 0.0 0.7373703251472494 3.774418126265609E-5 54-55 4.1518599388921706E-4 3.774418126265609E-5 0.0 0.8512822641979456 3.774418126265609E-5 56-57 4.1518599388921706E-4 3.774418126265609E-5 0.0 0.9853495960429 3.774418126265609E-5 58-59 4.1518599388921706E-4 3.774418126265609E-5 0.0 1.140213971763578 3.774418126265609E-5 60-61 4.1518599388921706E-4 3.774418126265609E-5 0.0 1.3160641122662926 3.774418126265609E-5 62-63 4.1518599388921706E-4 3.774418126265609E-5 0.0 1.4910650086905979 3.774418126265609E-5 64-65 4.1518599388921706E-4 3.774418126265609E-5 0.0 1.6631029868857843 3.774418126265609E-5 66-67 4.5293017515187314E-4 3.774418126265609E-5 0.0 1.861335426877254 3.774418126265609E-5 68-69 4.5293017515187314E-4 3.774418126265609E-5 0.0 2.090744560591678 3.774418126265609E-5 70-71 4.5293017515187314E-4 3.774418126265609E-5 0.0 2.34912235342519 3.774418126265609E-5 72-73 4.5293017515187314E-4 3.774418126265609E-5 0.0 2.607405785805546 3.774418126265609E-5 74-75 4.5293017515187314E-4 3.774418126265609E-5 0.0 2.8794847164374024 3.774418126265609E-5 76-77 4.5293017515187314E-4 3.774418126265609E-5 0.0 3.1641702036109858 3.774418126265609E-5 78-79 4.5293017515187314E-4 3.774418126265609E-5 0.0 3.507453532194843 5.6616271893984135E-5 80-81 5.284185376771853E-4 3.774418126265609E-5 0.0 3.8805170197949357 7.548836252531218E-5 82-83 5.284185376771853E-4 3.774418126265609E-5 0.0 4.247371589577321 7.548836252531218E-5 84-85 5.284185376771853E-4 3.774418126265609E-5 0.0 4.636495226304675 7.548836252531218E-5 86-87 5.284185376771853E-4 3.774418126265609E-5 0.0 5.039225640377216 7.548836252531218E-5 88-89 5.284185376771853E-4 3.774418126265609E-5 0.0 5.4685090859680345 7.548836252531218E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 10315 0.0 31.716478 1 GTATAGG 650 0.0 28.669905 1 TACATGG 12460 0.0 26.725655 2 GTCTACG 200 0.0010019023 25.882555 1 ACATGGG 12450 0.0 25.83658 3 CATGGGA 8470 0.0 23.920685 4 GAGTACT 9130 0.0 22.7155 12-13 GTACTTT 10085 0.0 20.662138 14-15 ATGGGAT 3775 0.0 20.499372 5 AGTACTT 10105 0.0 20.080124 12-13 CATGGGG 4180 0.0 19.998468 4 CTTTGCG 575 0.0 18.072245 2 AGTACAT 8985 0.0 17.978992 2 GTGTAGC 1120 0.0 17.563162 1 TTGCGTT 620 0.0 17.520073 4 ACTTTTT 12855 0.0 17.516396 16-17 ATGGGAG 3530 0.0 17.510973 5 TAGGACA 1445 0.0 17.32239 4 GTCCTAC 605 6.5125823E-7 17.11243 1 TACTTTT 12465 0.0 16.395174 14-15 >>END_MODULE