FastQCFastQC Report
Fri 27 May 2016
SRR939582_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939582_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2649415
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT150620.5685028581781261No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134390.5072440519888353No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103060.38899153209293375No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT77420.2922154513354835No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76950.2904414748161387No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT64000.241562760080999No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA57740.2179349026105763No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC56780.2143114612093613No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA49570.18709790651898628No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT41700.15739323586527593No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT40940.15452467808931405No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC37250.14059707520339396No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC35800.13512416892030882No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT31550.11908289188367997No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC30740.11602561320140485No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG27740.104702358822608No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA27430.10353228920346566No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG123950.026.9518451
TACATGG125800.026.4379792
ACATGGG126900.025.1645643
TACGCGG1204.5183697E-922.29297464-65
CATGGGA86900.022.146384
CATGGGG41250.021.497914
GAGTACT103150.020.83989712-13
ATGGGAG35550.019.9027925
AGTACTT108900.019.71789212-13
GTACTTT111350.019.22054114-15
ATGGGAT36150.018.2676245
ATGGGGA25400.017.642185
CTCGCGA850.001526358417.16678264-65
ATATAGG14750.016.9513232
ACTTTTT131700.016.59067216-17
TACTTTT131000.016.26548814-15
AGTACAT93050.015.9703322
CGATACG1205.8204285E-515.9368888-89
TAGGACA13950.015.2160054
ACGCATA9750.015.21534364-65
Produced by FastQC (version 0.11.5)