##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939582_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2649415 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.491189187046952 31.0 31.0 34.0 30.0 34.0 2 31.687987725592254 31.0 31.0 34.0 30.0 34.0 3 31.737763619516006 31.0 31.0 34.0 30.0 34.0 4 35.52705106599004 37.0 35.0 37.0 33.0 37.0 5 35.29345798978265 37.0 35.0 37.0 33.0 37.0 6 35.309955216528934 37.0 35.0 37.0 32.0 37.0 7 35.22095745664609 37.0 35.0 37.0 32.0 37.0 8 35.19043109516629 37.0 35.0 37.0 32.0 37.0 9 36.7739753870194 39.0 35.0 39.0 32.0 39.0 10-11 36.65521917102455 39.0 35.0 39.0 32.0 39.0 12-13 36.59877180434171 39.0 35.0 39.0 32.0 39.0 14-15 37.62919248211398 39.0 36.0 41.0 32.0 41.0 16-17 37.5958151139025 39.0 36.0 41.0 32.0 41.0 18-19 37.60530494467647 39.0 36.0 41.0 32.0 41.0 20-21 37.55475321910686 39.0 36.0 41.0 32.0 41.0 22-23 37.41597371495217 39.0 36.0 41.0 32.0 41.0 24-25 37.28153913222353 39.0 36.0 41.0 31.0 41.0 26-27 36.977724139102406 39.0 36.0 40.0 31.0 41.0 28-29 36.793177550515864 39.0 36.0 40.0 30.0 41.0 30-31 36.55475774840861 38.0 35.0 40.0 30.0 41.0 32-33 36.37299573679472 38.0 35.0 40.0 30.0 41.0 34-35 36.329001496556785 38.0 35.0 40.0 30.0 41.0 36-37 36.231876282122656 38.0 35.0 40.0 30.0 41.0 38-39 36.255763630839255 38.0 35.0 40.0 30.0 41.0 40-41 36.16578546584812 38.0 35.0 40.0 30.0 41.0 42-43 35.98765765272711 38.0 35.0 40.0 29.5 41.0 44-45 35.75298396061017 38.0 35.0 40.0 28.5 41.0 46-47 35.43322658020733 38.0 34.0 40.0 27.0 41.0 48-49 35.11513805877901 38.0 34.0 40.0 26.0 41.0 50-51 34.87363097136537 38.0 34.0 40.0 26.0 41.0 52-53 34.663122991301854 38.0 33.5 40.0 25.5 41.0 54-55 34.40214273717028 38.0 33.0 40.0 24.0 41.0 56-57 33.69715182408192 37.5 32.5 40.0 21.5 41.0 58-59 30.641169843154053 35.5 27.0 39.0 8.0 40.5 60-61 30.39818639209033 34.5 26.0 39.0 10.5 40.0 62-63 32.137745502309 35.5 30.5 39.0 18.5 40.0 64-65 32.18257200174378 36.0 31.0 39.0 15.5 40.0 66-67 27.341006599570093 32.0 19.0 37.0 4.0 39.5 68-69 19.710156770456877 20.0 8.0 32.0 2.0 37.0 70-71 17.752439123353646 15.5 7.5 29.0 2.0 35.0 72-73 19.504614414880265 20.0 7.5 31.0 2.0 35.5 74-75 21.026038578327668 23.5 9.5 32.5 2.0 36.0 76-77 22.914912159854158 27.5 12.0 33.0 2.0 35.5 78-79 25.190517906783196 30.5 14.0 34.5 2.0 36.5 80-81 26.788648060043442 32.5 23.5 35.0 2.0 37.0 82-83 27.821663650277515 33.0 26.5 35.0 2.0 36.0 84-85 27.826032727979573 34.0 27.0 35.0 2.0 36.0 86-87 27.565753383294048 34.0 27.0 35.0 2.0 36.0 88-89 27.11203359987016 33.0 26.0 35.0 2.0 35.5 90-91 25.36386994864904 32.5 16.0 34.5 2.0 35.0 92-93 23.86442327834635 31.5 4.0 34.0 2.0 35.0 94-95 19.98478135739399 28.0 2.0 34.0 2.0 35.0 96-97 17.25517538777428 18.5 2.0 33.0 2.0 35.0 98-99 17.558567079902545 22.0 2.0 33.0 2.0 35.0 100-101 16.757990160091943 17.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3154414469680553 1101 2 0.19566712410208353 1101 3 0.18346732027544377 1101 4 0.21477464689706238 1101 5 0.3641093790033878 1101 6 0.2725666180371107 1101 7 0.24967712099529393 1101 8 0.32575468801068297 1101 9 0.35754429872530125 1101 10-11 0.32320759087453865 1101 12-13 0.2713437677888493 1101 14-15 0.44515316251118975 1101 16-17 0.4494403339128965 1101 18-19 0.3981845127838355 1101 20-21 0.4175304243814324 1101 22-23 0.4503572353553409 1101 24-25 0.4334415316723508 1101 26-27 0.524777529186089 1101 28-29 0.4412413818609835 1101 30-31 0.15353554028637006 1101 32-33 0.3514327412545981 1101 34-35 0.20290067776615217 1101 36-37 0.08576651779988964 1101 38-39 0.31170847828627046 1101 40-41 0.37457582612407947 1101 42-43 0.4589054449254846 1101 44-45 0.2778228976972983 1101 46-47 0.16021761761240327 1101 48-49 0.13730381888762366 1101 50-51 0.1625833496879352 1101 52-53 0.20355185077094262 1101 54-55 0.16567238429647801 1101 56-57 -3.1802007913019175 1101 58-59 -10.201304081381572 1101 60-61 -11.685027626047503 1101 62-63 -3.750993947437891 1101 64-65 -0.9396828200180707 1101 66-67 -2.0284475879129715 1101 68-69 -4.5474270824066565 1101 70-71 -4.629821687130182 1101 72-73 -3.59357572690525 1101 74-75 -6.102175559220619 1101 76-77 -4.6570677356254855 1101 78-79 1.361205453121645 1101 80-81 1.9727727691320105 1101 82-83 0.4937278716995621 1101 84-85 0.16632554817256207 1101 86-87 0.1489267521500146 1101 88-89 0.08122152349640643 1101 90-91 1.430537670235843 1101 92-93 1.0949223982377134 1101 94-95 -8.420861651938784 1101 96-97 -9.593090570781113 1101 98-99 -8.946356900596866 1101 100-101 -9.719282119871362 1102 1 0.2383410498164551 1102 2 0.23762059496937837 1102 3 0.2552096372242083 1102 4 0.20419714291069369 1102 5 0.2656360852201445 1102 6 0.27180648373846594 1102 7 0.26686556497315195 1102 8 0.28611133212917395 1102 9 0.22747467339475946 1102 10-11 0.27992821799771406 1102 12-13 0.34067940545981656 1102 14-15 0.35851270851046024 1102 16-17 0.3818094273970374 1102 18-19 0.3877493792279907 1102 20-21 0.28296065544245863 1102 22-23 0.23667036116623308 1102 24-25 0.2588582437324902 1102 26-27 0.05958400444087175 1102 28-29 0.13341422699166827 1102 30-31 0.12418866154141028 1102 32-33 -0.029504724700295526 1102 34-35 0.12988940698006246 1102 36-37 -0.0618864747027601 1102 38-39 0.05878802745483114 1102 40-41 0.003668666500836082 1102 42-43 0.12074348432371096 1102 44-45 0.07513073475619336 1102 46-47 0.06791205848038828 1102 48-49 0.2961731253083997 1102 50-51 0.19191042037299866 1102 52-53 -0.03964903266852815 1102 54-55 -0.08035733769740716 1102 56-57 -3.401699469113918 1102 58-59 -10.103031344204812 1102 60-61 -8.141927763713518 1102 62-63 -2.4355543075484185 1102 64-65 -0.7576455115372482 1102 66-67 -2.1688892086084337 1102 68-69 -5.326549882474005 1102 70-71 -4.907055293484607 1102 72-73 -3.467415717397383 1102 74-75 -4.037120938495445 1102 76-77 -4.052023117970563 1102 78-79 1.7727327535802004 1102 80-81 2.131774541953373 1102 82-83 0.7767169574585573 1102 84-85 0.4560481362936706 1102 86-87 0.38176430625077984 1102 88-89 0.324556111340204 1102 90-91 1.7123989345843071 1102 92-93 1.1146363698527644 1102 94-95 -8.77939496414352 1102 96-97 -9.654022639039686 1102 98-99 -8.806604847986659 1102 100-101 -9.69418406997486 1103 1 0.21576873162369736 1103 2 0.21177539394169642 1103 3 0.2618975964669126 1103 4 0.20126576771654214 1103 5 0.13089855997166921 1103 6 0.22636530691042367 1103 7 0.2870303150649107 1103 8 0.23831849236553637 1103 9 0.36641602401493856 1103 10-11 0.2960835954669605 1103 12-13 0.3167088504673714 1103 14-15 0.3335607747993947 1103 16-17 0.47326663199292796 1103 18-19 0.3753597007799172 1103 20-21 0.34518796584494993 1103 22-23 0.36531665404610436 1103 24-25 0.3723098772571163 1103 26-27 0.4710830892976219 1103 28-29 0.3590760347702684 1103 30-31 0.4155092630809918 1103 32-33 0.14468240204157468 1103 34-35 0.17433036747941344 1103 36-37 0.2775119614110011 1103 38-39 0.3450911404002781 1103 40-41 0.39868666553342536 1103 42-43 0.2825761771783064 1103 44-45 0.2607249458978558 1103 46-47 0.11675858907874925 1103 48-49 0.150493986928538 1103 50-51 0.01342467254418267 1103 52-53 0.16164723797080427 1103 54-55 0.1356097852040179 1103 56-57 0.5458517631088498 1103 58-59 -8.19536723216114 1103 60-61 -7.420180532842203 1103 62-63 -2.094002307237293 1103 64-65 -0.3461762022577233 1103 66-67 -1.623516697907455 1103 68-69 -3.5669287250426276 1103 70-71 -4.008021845181085 1103 72-73 -3.11240515618244 1103 74-75 -3.990738488828324 1103 76-77 -0.6429139789344163 1103 78-79 3.252936856047075 1103 80-81 2.737100481220917 1103 82-83 1.2358271902914204 1103 84-85 0.7329044916171803 1103 86-87 0.6728673637771934 1103 88-89 0.586108427637047 1103 90-91 1.782471805752067 1103 92-93 1.3948774183419879 1103 94-95 -7.418175283580773 1103 96-97 -8.846850492451482 1103 98-99 -8.02913171284929 1103 100-101 -9.468242736369291 1104 1 0.13509046639176958 1104 2 0.1248549589771315 1104 3 0.1459403340703318 1104 4 0.0400845884812 1104 5 0.18751730064256833 1104 6 0.08212906839133893 1104 7 0.2964926013576843 1104 8 0.18362193516145453 1104 9 0.2774591767832675 1104 10-11 0.18965241381010145 1104 12-13 0.1738418545411875 1104 14-15 0.28935356108365795 1104 16-17 0.25001579389755335 1104 18-19 0.24887084359898637 1104 20-21 0.2106065659676588 1104 22-23 0.2501493071836691 1104 24-25 0.2416244476750009 1104 26-27 0.24966425403726333 1104 28-29 0.32040657985206167 1104 30-31 0.2249522440385121 1104 32-33 0.29448028052551223 1104 34-35 0.13470666787577557 1104 36-37 0.16834697060107828 1104 38-39 0.050556353889568584 1104 40-41 0.06039940949290923 1104 42-43 0.11709989485147076 1104 44-45 0.34411364011904055 1104 46-47 0.19174662344491367 1104 48-49 -0.006748135709770509 1104 50-51 -0.007652879125146228 1104 52-53 0.016225625146979894 1104 54-55 0.05117766767261145 1104 56-57 0.5731923596570638 1104 58-59 -8.477595869454973 1104 60-61 -7.628968902613522 1104 62-63 -2.0992832182353602 1104 64-65 -0.27534182099204685 1104 66-67 -1.758162059232859 1104 68-69 -3.146091120654372 1104 70-71 -3.835648915536339 1104 72-73 -2.975858486536474 1104 74-75 -3.5951559703772027 1104 76-77 -0.4665467153053662 1104 78-79 3.296158094855077 1104 80-81 2.74563908404237 1104 82-83 1.0375079568668681 1104 84-85 0.643525465427718 1104 86-87 0.6067786547596974 1104 88-89 0.5540827802653574 1104 90-91 2.1397678029678247 1104 92-93 1.8060125887549923 1104 94-95 -6.049992146089712 1104 96-97 -7.773196129429014 1104 98-99 -5.564794812704967 1104 100-101 -5.000237321523354 1105 1 0.17050115952188705 1105 2 0.18262677987460307 1105 3 0.20579869084355096 1105 4 0.18760856893705835 1105 5 0.2587136796346172 1105 6 0.25503308387983026 1105 7 0.26936161601033604 1105 8 0.20691997693169384 1105 9 0.22290023274099724 1105 10-11 0.20890616691725228 1105 12-13 0.26218847274726187 1105 14-15 0.2932753695343706 1105 16-17 0.2600122120469308 1105 18-19 0.3457553603739498 1105 20-21 0.20963132366466652 1105 22-23 0.3734273160290442 1105 24-25 0.4284996679917441 1105 26-27 0.3286589373625972 1105 28-29 0.32544944849422563 1105 30-31 0.41999843333103115 1105 32-33 0.4120675305000745 1105 34-35 0.33750533788943926 1105 36-37 0.28155304035018247 1105 38-39 0.3098001745726364 1105 40-41 0.25392291479142415 1105 42-43 0.3265658152576165 1105 44-45 0.24443530257016022 1105 46-47 0.1522138493981373 1105 48-49 0.22681135162905264 1105 50-51 0.18270868337031487 1105 52-53 0.21561557488751504 1105 54-55 0.3055415291846799 1105 56-57 0.9549965698419669 1105 58-59 -8.087311451890685 1105 60-61 -7.200614602848283 1105 62-63 -1.8693905851731252 1105 64-65 -0.12551348635044235 1105 66-67 -1.5043401882757834 1105 68-69 -3.3516096804097266 1105 70-71 -3.764660247539597 1105 72-73 -2.648191098048546 1105 74-75 -3.6690516837855434 1105 76-77 -0.03146259064878976 1105 78-79 3.9176563077909847 1105 80-81 3.3313967533364846 1105 82-83 1.8362235670125209 1105 84-85 1.5418767693394564 1105 86-87 1.3667190284168989 1105 88-89 1.2677197850296515 1105 90-91 2.5115441374059237 1105 92-93 2.3089289465239524 1105 94-95 -5.855791053227794 1105 96-97 -7.265492595366684 1105 98-99 -5.143557425270927 1105 100-101 -4.636142205559523 1106 1 0.13221978143093338 1106 2 0.22856788039479525 1106 3 0.18964234628914056 1106 4 0.17961138507484264 1106 5 0.24036639758486444 1106 6 0.1253108362981905 1106 7 0.18801151875437228 1106 8 0.25570574458308926 1106 9 0.15929643808150473 1106 10-11 0.24006522234196837 1106 12-13 0.25008486766237326 1106 14-15 0.17685740503466718 1106 16-17 0.275291541148448 1106 18-19 0.18603211601180902 1106 20-21 0.1510148666324227 1106 22-23 0.20584813718985373 1106 24-25 0.1289751066683138 1106 26-27 0.2073223385639551 1106 28-29 0.29934602646295616 1106 30-31 0.41116062349065885 1106 32-33 0.4480451346123928 1106 34-35 0.35001755310832294 1106 36-37 0.19849030885489327 1106 38-39 0.24570235640402416 1106 40-41 0.33268627351315416 1106 42-43 0.35423078242907025 1106 44-45 0.31368290098127716 1106 46-47 0.3363869780592097 1106 48-49 0.3134535847527218 1106 50-51 0.2266661790664557 1106 52-53 0.2591726699151593 1106 54-55 0.32855528102918896 1106 56-57 0.9382558394347598 1106 58-59 -8.385018227948578 1106 60-61 -7.3492399889842766 1106 62-63 -1.9450589754005811 1106 64-65 -0.1962092111868543 1106 66-67 0.7748295564552521 1106 68-69 -2.27929377971142 1106 70-71 -2.4570334095508954 1106 72-73 -1.1140520066465704 1106 74-75 -1.0558422229413154 1106 76-77 2.2367639365679786 1106 78-79 4.588316423342999 1106 80-81 3.477225914010045 1106 82-83 1.8455042804278854 1106 84-85 1.4278362311152542 1106 86-87 1.2430383652131916 1106 88-89 1.1691609596097017 1106 90-91 2.4883859921256324 1106 92-93 2.209603030976414 1106 94-95 -5.784411264489364 1106 96-97 -7.602226529966703 1106 98-99 -5.357936321564766 1106 100-101 -4.6592734517400825 1107 1 0.007654623509587566 1107 2 0.03342781559411989 1107 3 0.03848767305698786 1107 4 0.04048950694028264 1107 5 0.09409832317143696 1107 6 0.04088412142924369 1107 7 0.010373638408232466 1107 8 0.08911214315918414 1107 9 0.016351273950192535 1107 10-11 0.00469535407871291 1107 12-13 -0.029332145573626178 1107 14-15 0.013319904558528606 1107 16-17 0.07847068719698314 1107 18-19 0.029559204979420883 1107 20-21 0.07487124552822877 1107 22-23 0.17111455984400692 1107 24-25 0.1601313150785515 1107 26-27 0.09205101026554274 1107 28-29 -0.027141451447221243 1107 30-31 -0.01054431297258418 1107 32-33 0.02717398453315667 1107 34-35 0.05895500210404947 1107 36-37 0.11651555041459716 1107 38-39 0.1401257354348786 1107 40-41 -0.023787156940393572 1107 42-43 -0.04746045285832423 1107 44-45 -0.11161752644462553 1107 46-47 -0.14395847163831377 1107 48-49 -0.06843893200438345 1107 50-51 -0.08148042236091158 1107 52-53 -0.003463577140010443 1107 54-55 0.008092857687096 1107 56-57 0.4998107670678493 1107 58-59 -8.583549420343324 1107 60-61 -7.950133912795579 1107 62-63 -2.340024803259791 1107 64-65 -0.6374201435016644 1107 66-67 0.48111570672494963 1107 68-69 -2.5716443797582826 1107 70-71 -2.5916715288307692 1107 72-73 -1.0061743499516353 1107 74-75 -1.0249505730404103 1107 76-77 2.2726297366093 1107 78-79 4.329874732984955 1107 80-81 3.192439586560024 1107 82-83 1.5465257509899786 1107 84-85 1.051199159911846 1107 86-87 0.812818153727985 1107 88-89 0.7798646713932875 1107 90-91 2.047151570431833 1107 92-93 1.7445203679849364 1107 94-95 -7.015936781342273 1107 96-97 -8.528180273781263 1107 98-99 -6.404918888636466 1107 100-101 -5.659333127459682 1108 1 -0.0423567737230961 1108 2 -0.1182259171980462 1108 3 -0.10035001333879734 1108 4 -0.09336651142230323 1108 5 -0.1287346999984038 1108 6 -0.10752826106869406 1108 7 -0.1266258614743947 1108 8 -0.08727687331066392 1108 9 -0.21375624561505902 1108 10-11 -0.15455670394397458 1108 12-13 -0.1673968047396741 1108 14-15 -0.2491721428947855 1108 16-17 -0.16733263625874883 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-0.5222630019764054 2312 36-37 -0.46740606975237853 2312 38-39 -0.5679976295658165 2312 40-41 -0.42107102411273445 2312 42-43 -0.4597168457838663 2312 44-45 -0.6022274672404322 2312 46-47 -0.6642844075776253 2312 48-49 -0.6576532561629449 2312 50-51 -0.557147877582274 2312 52-53 -0.6488198661765949 2312 54-55 -0.47246739164638285 2312 56-57 0.11079858827871902 2312 58-59 3.1899989506028135 2312 60-61 3.144667258771392 2312 62-63 0.441398475375383 2312 64-65 -0.1558504189951364 2312 66-67 -2.2573419804316224 2312 68-69 -4.887478683139054 2312 70-71 -4.603071776662244 2312 72-73 -7.249780172739614 2312 74-75 -7.745998099106522 2312 76-77 -5.075925205625939 2312 78-79 -0.36556917323962423 2312 80-81 1.0756640242454303 2312 82-83 -0.030129900944483978 2312 84-85 -0.28660202417186653 2312 86-87 -0.21458161846732438 2312 88-89 -0.1018690224221892 2312 90-91 1.5720285934501206 2312 92-93 2.9023414536870646 2312 94-95 5.030241231750239 2312 96-97 0.40298641166051397 2312 98-99 -0.8621103822591856 2312 100-101 -0.2551813244231216 2313 1 -0.329714954216886 2313 2 -0.3240039719680752 2313 3 -0.27232533000035275 2313 4 -0.20125278442451844 2313 5 -0.18905533650933393 2313 6 -0.21533975078639855 2313 7 -0.2480631661507431 2313 8 -0.41608464282003155 2313 9 -0.2463792109491365 2313 10-11 -0.3123548089563499 2313 12-13 -0.3303556498915725 2313 14-15 -0.40963060257179507 2313 16-17 -0.43602614569380904 2313 18-19 -0.5318899930174794 2313 20-21 -0.42768820854117706 2313 22-23 -0.42681994419893243 2313 24-25 -0.45019443152035166 2313 26-27 -0.611091256603558 2313 28-29 -0.5574124755754895 2313 30-31 -0.5993067948030131 2313 32-33 -0.545247091937334 2313 34-35 -0.4452300652244645 2313 36-37 -0.5580694669981554 2313 38-39 -0.6439378470844375 2313 40-41 -0.5895519889715288 2313 42-43 -0.5952870371639776 2313 44-45 -0.5757258944994561 2313 46-47 -0.682708358981202 2313 48-49 -0.6878209239670241 2313 50-51 -0.6618448074610583 2313 52-53 -0.445424596623333 2313 54-55 -0.5411445403148818 2313 56-57 0.23449679458160944 2313 58-59 3.3096950814118173 2313 60-61 3.2225569630181496 2313 62-63 0.3909904510910813 2313 64-65 -0.17732063549549792 2313 66-67 -2.1209274889009535 2313 68-69 -4.776361650844805 2313 70-71 -3.574983024015978 2313 72-73 -3.069363320415828 2313 74-75 -3.735500247316917 2313 76-77 0.31329710579417025 2313 78-79 2.7440558948668716 2313 80-81 2.0086192278597785 2313 82-83 0.33264766562939485 2313 84-85 0.12884914408207848 2313 86-87 0.259696800640949 2313 88-89 0.3009135264503797 2313 90-91 1.746977558521376 2313 92-93 3.129513978304562 2313 94-95 5.343063077691905 2313 96-97 1.2113232029905738 2313 98-99 2.877855203231654 2313 100-101 3.9316249548504913 2314 1 -0.48990397404900676 2314 2 -0.4500582678919649 2314 3 -0.479694145124828 2314 4 -0.33004076358999157 2314 5 -0.4462810172079017 2314 6 -0.4508673703222499 2314 7 -0.4462544182198229 2314 8 -0.5555273014802609 2314 9 -0.5603059027069861 2314 10-11 -0.6882293888460183 2314 12-13 -0.7213611876026746 2314 14-15 -0.8885774473388182 2314 16-17 -0.8138066550803629 2314 18-19 -0.7523324269122114 2314 20-21 -0.7433541226971414 2314 22-23 -0.8218160330865842 2314 24-25 -0.8055753006280639 2314 26-27 -0.9835973653828276 2314 28-29 -0.8113256356314054 2314 30-31 -0.7475078999361742 2314 32-33 -0.7352754779163106 2314 34-35 -0.7125665194174644 2314 36-37 -0.7604815765825279 2314 38-39 -0.7857104904013497 2314 40-41 -0.889070622223862 2314 42-43 -1.0257101311080064 2314 44-45 -0.8700811849082797 2314 46-47 -0.8875839152637539 2314 48-49 -0.8233834869489414 2314 50-51 -0.8473631882436763 2314 52-53 -0.769592693269125 2314 54-55 -0.8191291695349321 2314 56-57 0.010004727261808455 2314 58-59 3.1020335652396582 2314 60-61 3.076606463944799 2314 62-63 0.44956673633175015 2314 64-65 -0.40414076022227974 2314 66-67 -2.129996704387459 2314 68-69 -5.036576194690099 2314 70-71 -3.79158962874887 2314 72-73 -2.9676085113857944 2314 74-75 -3.957163955253794 2314 76-77 0.5518102157897786 2314 78-79 3.268097371207592 2314 80-81 2.164033908154348 2314 82-83 0.6123155667797953 2314 84-85 0.2152599739726604 2314 86-87 0.35815504906488016 2314 88-89 0.5330938746224909 2314 90-91 2.019325042605306 2314 92-93 3.7043022277667994 2314 94-95 6.2264458610403715 2314 96-97 3.6029339573329686 2314 98-99 5.733857550139973 2314 100-101 6.2224629543364 2315 1 -0.49768057619587225 2315 2 -0.42803974100155173 2315 3 -0.46516050121550734 2315 4 -0.29703672372922085 2315 5 -0.5236057231865274 2315 6 -0.51907126061203 2315 7 -0.5015540105096719 2315 8 -0.5922660560104234 2315 9 -0.7117814912089173 2315 10-11 -0.42314755230983536 2315 12-13 -0.5242367430946118 2315 14-15 -0.6317320221426215 2315 16-17 -0.6455363141286767 2315 18-19 -0.6654840338566714 2315 20-21 -0.7551262513945645 2315 22-23 -0.6646966984140192 2315 24-25 -0.5535348181653461 2315 26-27 -0.6642828078871119 2315 28-29 -0.6042103480397572 2315 30-31 -0.511827504566142 2315 32-33 -0.5774231719225398 2315 34-35 -0.5718310856470339 2315 36-37 -0.47855976669247724 2315 38-39 -0.704386267792394 2315 40-41 -0.7265865936265143 2315 42-43 -0.6453071989187151 2315 44-45 -0.7181510439849959 2315 46-47 -0.6700483126686052 2315 48-49 -0.8635260962001752 2315 50-51 -0.7858044274788583 2315 52-53 -0.8061682978707125 2315 54-55 -0.6393950127166903 2315 56-57 0.11646309571946034 2315 58-59 3.0992364559827905 2315 60-61 3.215126960097919 2315 62-63 0.5214648071471792 2315 64-65 -0.04349283966818973 2315 66-67 -1.8745702275607705 2315 68-69 -4.927741195382346 2315 70-71 -3.8337873824226243 2315 72-73 -3.1133262247686133 2315 74-75 -0.8465229065037541 2315 76-77 1.7028505055886392 2315 78-79 3.401431270330015 2315 80-81 2.294692173781936 2315 82-83 0.47959911666890065 2315 84-85 0.2830915834859731 2315 86-87 0.24613368605109542 2315 88-89 0.44593697873228777 2315 90-91 2.087475415158419 2315 92-93 3.6681129642227894 2315 94-95 6.374700251806491 2315 96-97 4.01696661189251 2315 98-99 6.203063254459352 2315 100-101 6.402579272128648 2316 1 -0.484009734882374 2316 2 -0.47117334531417043 2316 3 -0.5264616372305291 2316 4 -0.43386217563809026 2316 5 -0.48650232068332144 2316 6 -0.6598637595745842 2316 7 -0.43664868722469663 2316 8 -0.47405517905749406 2316 9 -0.6631617108644434 2316 10-11 -0.6399297513324953 2316 12-13 -0.5702036885475508 2316 14-15 -0.7494365447609965 2316 16-17 -0.7568733072877762 2316 18-19 -0.7651019095171634 2316 20-21 -0.7383840988971357 2316 22-23 -0.8071014823574032 2316 24-25 -0.6637617902587962 2316 26-27 -0.7030997662380685 2316 28-29 -0.7128609252836995 2316 30-31 -0.6852286948781767 2316 32-33 -0.6115582494458778 2316 34-35 -0.5637525733457522 2316 36-37 -0.6294760536208841 2316 38-39 -0.8483138093117972 2316 40-41 -0.7667918535099929 2316 42-43 -0.8835969549911695 2316 44-45 -0.8020606536336388 2316 46-47 -0.8078755214785787 2316 48-49 -0.7759349771603894 2316 50-51 -0.6852198273800312 2316 52-53 -0.6300108906160276 2316 54-55 -0.5776069188719148 2316 56-57 0.2083713885169587 2316 58-59 3.412826280602758 2316 60-61 3.2624443377168575 2316 62-63 0.5195529979085016 2316 64-65 0.1294481124890794 2316 66-67 -2.058559534412815 2316 68-69 -2.9249307473956563 2316 70-71 -3.445131170955859 2316 72-73 -1.02860954898647 2316 74-75 5.411156690946196 2316 76-77 5.585845378709767 2316 78-79 4.626721937510403 2316 80-81 2.9940304494854395 2316 82-83 1.1804244577406031 2316 84-85 0.7908827108385417 2316 86-87 0.6778059352605545 2316 88-89 0.6147076163824856 2316 90-91 2.3732147617601065 2316 92-93 3.9410388741926567 2316 94-95 6.245907130044603 2316 96-97 3.356271187818276 2316 98-99 5.687104945069269 2316 100-101 5.811998716267041 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 12.0 4 31.0 5 117.0 6 300.0 7 646.0 8 1364.0 9 2500.0 10 4045.0 11 6357.0 12 9169.0 13 12853.0 14 15748.0 15 20876.0 16 27912.0 17 36690.0 18 39141.0 19 36606.0 20 38336.0 21 39268.0 22 39082.0 23 39855.0 24 42605.0 25 45821.0 26 56744.0 27 79963.0 28 111663.0 29 147971.0 30 181588.0 31 207469.0 32 220578.0 33 220566.0 34 225790.0 35 239103.0 36 240672.0 37 182436.0 38 70961.0 39 4576.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.406891719245046 20.629249183764077 14.648980799956506 22.31487829703437 2 15.03113744832603 22.48993133793446 39.41589114029462 23.063040073444892 3 19.10673730878297 26.9278341487976 30.951483178714064 23.013945363705368 4 12.153097948037587 17.77935883959289 39.13018534280209 30.937357869567432 5 12.112213053427649 39.48856179343284 35.117603670136404 13.281621483003109 6 29.182970580297912 38.68370942264613 17.997293742203468 14.136026254852485 7 26.26859136828319 34.32965390472991 22.26314110850886 17.13861361847804 8 24.989063623479147 34.972248590726636 20.68992588930009 19.348761896494132 9 25.82498400590319 17.12181745781616 21.64073955948766 35.41245897679299 10-11 23.952646150187874 27.117948679236736 28.47836220448665 20.45104296608874 12-13 25.808093484788152 25.005576702781557 28.359807731140645 20.826522081289646 14-15 22.590590185566263 26.166149065978612 27.661164548706047 23.582096199749074 16-17 20.796368405170274 29.355618005412136 28.193115875224507 21.65489771419308 18-19 20.55909502750899 28.686953096853955 30.268797936443505 20.48515393919355 20-21 21.911270978687746 27.20381669160928 29.759173251453625 21.12573907824935 22-23 21.34875057323975 27.28022978657553 29.738319591306006 21.632700048878714 24-25 21.709563590123548 27.40759934370368 29.82500274589126 21.05783432028151 26-27 21.555470253577116 26.998227153129505 30.496382026042767 20.949920567250608 28-29 20.89163920751569 27.782300831512156 30.469806257289413 20.856253703682732 30-31 21.247364136860867 27.43851193977823 29.987492802274733 21.32663112108617 32-33 21.149872993147348 27.561553358259726 29.977014392474295 21.311559256118628 34-35 21.789179333693294 27.215478574020835 29.852468449521215 21.142873642764652 36-37 21.688429708199763 26.85880250476618 30.303602126682566 21.149165660351485 38-39 21.389404892882606 27.528277386118415 30.283741860541983 20.798575860456992 40-41 21.186611478363627 27.541816699555376 30.25513036451593 21.016441457565065 42-43 20.888863115140715 27.830245615214857 29.447833755294507 21.833057514349925 44-45 21.365570362847926 27.485705887731054 29.3223302482079 21.826393501213122 46-47 21.687920852524815 27.825019795935635 28.95047918253364 21.536580169005912 48-49 21.62230768925667 27.744449076024885 28.990556171805487 21.64268706291296 50-51 21.212766198545022 27.94312284227146 29.360859087578284 21.483251871605237 52-53 21.54575337612825 28.37239393755704 28.583724751387457 21.498127934927254 54-55 21.125486180566977 27.76444833575617 29.009225417281186 22.100840066395662 56-57 21.555413031645973 28.07480541142636 28.561319338291202 21.808462218636464 58-59 20.998742573186217 27.38324973622178 29.93141671291936 21.68659097767264 60-61 20.89646848445903 27.812704244072272 30.03622441143144 21.25460286003726 62-63 21.50645898664267 27.81070851067363 29.188810613392356 21.494021889291336 64-65 21.181027303065708 28.224139125209557 28.997931382908554 21.59690218881618 66-67 21.32836176465872 27.582260716055547 28.98370705999403 22.1056704592917 68-69 20.746791967029342 27.320263587290317 30.260869397004903 21.67207504867544 70-71 21.8716270853338 27.263050508980196 29.08101646765463 21.784305938031377 72-73 21.19180566540653 27.145045783578293 29.62726434826787 22.0358842027473 74-75 20.804094624208712 28.069046004275783 29.276463637660722 21.850395733854782 76-77 19.883242672524943 27.790891118029954 29.77268111306269 22.553185096382418 78-79 21.015948846460038 27.738108028179546 29.070323209859577 22.17561991550084 80-81 21.175065493708146 28.190330870664184 28.433142799317007 22.201460836310662 82-83 21.358210803000816 27.890943348939313 28.530840050547063 22.22000579751281 84-85 21.010845340987434 27.88468198203622 28.4004428949872 22.704029781989153 86-87 21.064894585128872 28.12539042020632 28.432480092579837 22.37723490208497 88-89 21.25231820087042 27.741148091399058 28.134782232074297 22.871751475656225 90-91 20.996648977347267 27.442822797255555 28.70464492339762 22.855883301999555 92-93 21.010219721269344 28.111739244632254 28.586933719188877 22.291107314909524 94-95 20.035811455014766 27.76537130653866 29.145812549105422 23.053004689341154 96-97 19.59019353689193 27.862546394153554 29.04324412228722 23.50401594666729 98-99 20.22520598826876 27.781611020894804 28.529612689622052 23.463570301214386 100-101 19.43932082148403 27.65448387672424 29.304438124817278 23.60175717697445 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1455.0 1 1103.5 2 743.0 3 776.0 4 820.0 5 949.5 6 1252.5 7 1821.5 8 2569.0 9 3135.5 10 3309.5 11 3361.0 12 3534.5 13 3714.5 14 3913.5 15 4150.0 16 4506.0 17 4833.5 18 5144.0 19 5613.5 20 6146.5 21 6938.5 22 8002.0 23 9354.5 24 11078.5 25 13567.0 26 17219.0 27 21336.5 28 25341.5 29 29951.0 30 35605.0 31 41927.5 32 49765.5 33 57596.5 34 64761.0 35 72195.5 36 79690.0 37 85453.5 38 87985.5 39 89400.5 40 89953.0 41 90015.0 42 90831.0 43 92621.0 44 94976.5 45 98415.0 46 104147.5 47 112564.0 48 120552.0 49 114891.5 50 98909.0 51 87014.0 52 78865.0 53 73188.0 54 68211.0 55 62195.5 56 55974.0 57 49650.0 58 43422.5 59 36963.5 60 30284.0 61 23798.5 62 18106.5 63 13492.5 64 9866.0 65 7136.5 66 4846.5 67 3320.5 68 2243.0 69 1378.0 70 859.5 71 546.5 72 338.0 73 221.5 74 142.5 75 76.0 76 41.5 77 27.5 78 17.0 79 7.0 80 3.5 81 2.0 82 3.0 83 3.0 84 0.5 85 0.5 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02532634562724224 2 0.013361440166980258 3 1.5097672505062437E-4 4 0.0 5 0.06288180598358506 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 4.1518599388921706E-4 16-17 1.8872090631328046E-5 18-19 3.208255407325768E-4 20-21 0.0 22-23 0.0 24-25 7.548836252531218E-5 26-27 1.5097672505062437E-4 28-29 9.436045315664023E-4 30-31 0.005303057467403182 32-33 0.005605010917504431 34-35 0.0018683369725014769 36-37 0.08988776767701549 38-39 0.08313155923100005 40-41 0.08894416314544909 42-43 0.09245437200287611 44-45 0.09515308096315601 46-47 0.09085024429921322 48-49 0.0804139781800888 50-51 0.07378987436849266 52-53 0.063051654799267 54-55 0.05097351679521706 56-57 0.07194040948662252 58-59 0.748787939979203 60-61 0.3527005018088899 62-63 0.003132767044800456 64-65 0.0022835229663906937 66-67 1.1368924838124643 68-69 2.4417654463343794 70-71 2.398944672691896 72-73 1.8608447525208394 74-75 1.327009924832463 76-77 0.7624702056869157 78-79 0.5777124384062142 80-81 0.040348529769779366 82-83 1.1323254378796828E-4 84-85 0.0010757091659856987 86-87 9.813487128290585E-4 88-89 1.8872090631328046E-5 90-91 0.3407167242579966 92-93 0.054596958196432045 94-95 0.9901053628819947 96-97 1.7073391673256173 98-99 0.6593531024773394 100-101 0.5905077158542547 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2649415.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.24839013178985 #Duplication Level Percentage of deduplicated Percentage of total 1 68.96804670260818 28.448208970166846 2 14.897228410085361 12.289733786831688 3 6.0271711108876405 7.458333161188397 4 3.102361769592198 5.118697144083556 5 1.7920529084986634 3.6959648753280776 6 1.1226405022865384 2.778426804963819 7 0.7859986985732891 2.2694826673281083 8 0.536554138800196 1.7705595555245601 9 0.4078491280643439 1.5140807954377538 >10 2.0892249817035946 15.609626906673052 >50 0.16347062917640237 4.720135514267894 >100 0.09862081819099276 7.447836714648108 >500 0.004755942496883935 1.2736660028260827 >1k 0.003292575574764109 2.885356572024052 >5k 4.573021631616818E-4 1.255859351561265 >10k+ 2.743812978970091E-4 1.4640311771467454 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 15062 0.5685028581781261 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13439 0.5072440519888353 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10306 0.38899153209293375 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 7742 0.2922154513354835 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7695 0.2904414748161387 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 6400 0.241562760080999 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5774 0.2179349026105763 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 5678 0.2143114612093613 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4957 0.18709790651898628 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4170 0.15739323586527593 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 4094 0.15452467808931405 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3725 0.14059707520339396 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3580 0.13512416892030882 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3155 0.11908289188367997 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 3074 0.11602561320140485 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2774 0.104702358822608 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2743 0.10353228920346566 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 3.774418126265609E-5 0.0 16-17 0.0 0.0 0.0 2.6420926883859267E-4 0.0 18-19 1.8872090631328046E-5 0.0 0.0 4.340580845205451E-4 0.0 20-21 3.774418126265609E-5 0.0 0.0 5.661627189398414E-4 0.0 22-23 3.774418126265609E-5 0.0 0.0 0.0010568370753543707 0.0 24-25 1.1323254378796828E-4 0.0 0.0 0.0022080346038653815 0.0 26-27 1.1323254378796828E-4 0.0 0.0 0.0029440461384871754 0.0 28-29 1.5097672505062437E-4 0.0 0.0 0.006171173636444272 0.0 30-31 1.5097672505062437E-4 0.0 0.0 0.015041056233168454 0.0 32-33 1.5097672505062437E-4 0.0 0.0 0.03212029825452034 0.0 34-35 1.6984881568195243E-4 0.0 0.0 0.05674837652840344 0.0 36-37 1.8872090631328047E-4 0.0 0.0 0.09360556953138713 0.0 38-39 1.8872090631328047E-4 0.0 0.0 0.14918387644064823 0.0 40-41 1.8872090631328047E-4 0.0 0.0 0.21097110116761625 0.0 42-43 1.8872090631328047E-4 0.0 0.0 0.2778160461837802 0.0 44-45 2.2646508757593657E-4 0.0 0.0 0.35487079223149265 0.0 46-47 3.019534501012488E-4 0.0 0.0 0.44991064065086067 0.0 48-49 3.3969763136390487E-4 3.774418126265609E-5 0.0 0.5416667453003776 0.0 50-51 3.3969763136390487E-4 3.774418126265609E-5 0.0 0.6403111630303293 0.0 52-53 3.3969763136390487E-4 3.774418126265609E-5 0.0 0.7346149999150756 0.0 54-55 3.3969763136390487E-4 3.774418126265609E-5 0.0 0.8388266843812691 0.0 56-57 3.7744181262656094E-4 3.774418126265609E-5 0.0 0.9379617764676353 0.0 58-59 3.7744181262656094E-4 3.774418126265609E-5 0.0 1.0149032899715598 0.0 60-61 3.7744181262656094E-4 3.774418126265609E-5 0.0 1.0807480141842634 1.8872090631328046E-5 62-63 3.7744181262656094E-4 3.774418126265609E-5 0.0 1.1377039837096112 3.774418126265609E-5 64-65 3.7744181262656094E-4 3.774418126265609E-5 0.0 1.1997176735241553 3.774418126265609E-5 66-67 3.7744181262656094E-4 3.774418126265609E-5 0.0 1.2526350156543993 3.774418126265609E-5 68-69 3.7744181262656094E-4 3.774418126265609E-5 0.0 1.3167812517102833 3.774418126265609E-5 70-71 3.7744181262656094E-4 3.774418126265609E-5 0.0 1.4219742848893058 3.774418126265609E-5 72-73 3.7744181262656094E-4 3.774418126265609E-5 0.0 1.5602123487637836 3.774418126265609E-5 74-75 3.7744181262656094E-4 3.774418126265609E-5 0.0 1.7504430223275704 3.774418126265609E-5 76-77 3.7744181262656094E-4 3.774418126265609E-5 0.0 1.9872122713882119 3.774418126265609E-5 78-79 3.7744181262656094E-4 3.774418126265609E-5 0.0 2.305093765982302 3.774418126265609E-5 80-81 4.1518599388921706E-4 3.774418126265609E-5 0.0 2.6774401141384043 3.774418126265609E-5 82-83 4.1518599388921706E-4 3.774418126265609E-5 0.0 3.030895499572547 3.774418126265609E-5 84-85 4.1518599388921706E-4 3.774418126265609E-5 0.0 3.363327376043391 3.774418126265609E-5 86-87 4.1518599388921706E-4 3.774418126265609E-5 0.0 3.660241977946075 3.774418126265609E-5 88-89 4.1518599388921706E-4 3.774418126265609E-5 0.0 3.9492303017836017 3.774418126265609E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 12395 0.0 26.951845 1 TACATGG 12580 0.0 26.437979 2 ACATGGG 12690 0.0 25.164564 3 TACGCGG 120 4.5183697E-9 22.292974 64-65 CATGGGA 8690 0.0 22.14638 4 CATGGGG 4125 0.0 21.49791 4 GAGTACT 10315 0.0 20.839897 12-13 ATGGGAG 3555 0.0 19.902792 5 AGTACTT 10890 0.0 19.717892 12-13 GTACTTT 11135 0.0 19.220541 14-15 ATGGGAT 3615 0.0 18.267624 5 ATGGGGA 2540 0.0 17.64218 5 CTCGCGA 85 0.0015263584 17.166782 64-65 ATATAGG 1475 0.0 16.951323 2 ACTTTTT 13170 0.0 16.590672 16-17 TACTTTT 13100 0.0 16.265488 14-15 AGTACAT 9305 0.0 15.970332 2 CGATACG 120 5.8204285E-5 15.93688 88-89 TAGGACA 1395 0.0 15.216005 4 ACGCATA 975 0.0 15.215343 64-65 >>END_MODULE