##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939581_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1843954 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.173622552406403 31.0 30.0 33.0 26.0 34.0 2 30.251371780424023 31.0 30.0 34.0 26.0 34.0 3 30.287592315209597 31.0 30.0 34.0 26.0 34.0 4 33.85598284989756 35.0 35.0 37.0 28.0 37.0 5 33.88376933480987 35.0 35.0 37.0 28.0 37.0 6 34.03072690533495 36.0 35.0 37.0 29.0 37.0 7 33.84623043741872 35.0 35.0 37.0 28.0 37.0 8 33.85932078565951 35.0 35.0 37.0 28.0 37.0 9 35.063656143266044 37.0 35.0 39.0 28.0 39.0 10-11 34.95944502953978 37.0 34.0 39.0 27.0 39.0 12-13 34.813169417458354 37.0 34.0 39.0 27.0 39.0 14-15 35.349787738739685 38.0 34.0 40.0 27.0 41.0 16-17 35.1417952942427 38.0 33.0 40.0 27.0 41.0 18-19 35.22401562078013 38.0 33.5 40.0 27.0 41.0 20-21 35.36662953631164 38.0 34.0 40.0 27.0 41.0 22-23 35.225259144208586 38.0 34.0 40.0 26.0 41.0 24-25 35.05727827266841 38.0 34.0 40.0 25.0 41.0 26-27 35.113442905842554 38.0 34.0 40.0 25.5 41.0 28-29 34.98608533618518 38.0 34.0 40.0 25.0 41.0 30-31 34.73543862807857 38.0 34.0 40.0 24.5 41.0 32-33 34.43539724960601 38.0 33.0 40.0 24.0 41.0 34-35 34.15193112192603 38.0 33.0 40.0 23.5 41.0 36-37 33.7788982263115 37.5 33.0 40.0 21.5 41.0 38-39 33.55970349585727 37.0 32.5 40.0 20.0 41.0 40-41 33.28134948051849 37.0 31.5 40.0 18.5 41.0 42-43 32.89928924474255 37.0 31.0 40.0 16.0 41.0 44-45 32.55634224064158 37.0 31.0 40.0 14.5 41.0 46-47 31.962938609097627 37.0 30.0 40.0 9.0 41.0 48-49 31.4937935002717 36.0 30.0 39.0 7.5 41.0 50-51 30.80551168846945 35.5 28.5 38.5 6.5 40.0 52-53 30.83652927350682 35.5 29.0 39.0 2.0 40.0 54-55 30.871012237832396 36.0 29.0 39.0 2.0 40.0 56-57 30.705924876650933 35.5 28.5 39.0 2.0 40.0 58-59 30.300593181825576 35.0 28.0 39.0 2.0 40.0 60-61 29.790377091836348 35.0 27.5 38.5 2.0 40.0 62-63 29.393429554099505 34.0 27.0 38.0 2.0 40.0 64-65 28.745677224052226 34.0 26.0 38.0 2.0 40.0 66-67 28.14849882372337 34.0 25.5 37.0 2.0 39.0 68-69 27.634556230795347 33.0 24.5 36.5 2.0 39.0 70-71 27.0309318996027 32.5 23.0 36.0 2.0 39.0 72-73 26.264005230065393 32.0 21.5 35.0 2.0 38.0 74-75 25.828008724729575 31.5 20.0 35.0 2.0 37.0 76-77 25.4591085243992 31.0 19.0 35.0 2.0 37.0 78-79 24.861367745616214 31.0 17.5 34.5 2.0 36.0 80-81 24.163595187298597 31.0 10.0 34.0 2.0 36.0 82-83 23.496894716462556 30.0 7.0 34.0 2.0 35.0 84-85 22.828297506336924 29.5 2.0 34.0 2.0 35.0 86-87 22.175712897393318 29.0 2.0 34.0 2.0 35.0 88-89 21.33376130857928 29.0 2.0 34.0 2.0 35.0 90-91 20.066071875979553 26.5 2.0 33.0 2.0 34.5 92-93 18.51443528417737 24.0 2.0 32.0 2.0 34.0 94-95 18.22485674805337 24.0 2.0 32.0 2.0 34.0 96-97 17.545789916668202 23.0 2.0 32.0 2.0 34.0 98-99 16.597207142911373 18.0 2.0 32.0 2.0 34.0 100-101 15.025521515178795 2.0 2.0 30.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.0035524325801681 1101 2 1.0142737030270013 1101 3 1.0625413412822269 1101 4 0.9752310068487091 1101 5 0.8940315063561641 1101 6 0.904726432304976 1101 7 0.9678998548666087 1101 8 0.9340023105964477 1101 9 1.2670189453186396 1101 10-11 1.084060379679947 1101 12-13 1.1047035196861756 1101 14-15 1.261429293036251 1101 16-17 1.0525592475449628 1101 18-19 1.1060662045507357 1101 20-21 1.4693562814776016 1101 22-23 1.5241585805424762 1101 24-25 1.4059954225572753 1101 26-27 1.3576351687378363 1101 28-29 1.534246917319102 1101 30-31 1.5990142068515212 1101 32-33 1.4221930839898107 1101 34-35 1.2953662134853516 1101 36-37 1.158088997248008 1101 38-39 1.24620309765168 1101 40-41 1.4494906951071798 1101 42-43 1.3527926286797154 1101 44-45 1.2571743173571477 1101 46-47 1.3556760607276672 1101 48-49 1.2317958153314237 1101 50-51 1.2735175635619456 1101 52-53 1.3070330749779409 1101 54-55 1.4134019895043402 1101 56-57 1.2551106435965416 1101 58-59 1.0489328758853915 1101 60-61 0.9937316896400361 1101 62-63 1.0151003230580677 1101 64-65 1.2044192338456376 1101 66-67 1.0349930005396004 1101 68-69 1.0200443665290209 1101 70-71 0.8788417883724549 1101 72-73 1.2109601022545498 1101 74-75 1.0324817651299618 1101 76-77 0.484875031849203 1101 78-79 0.6383466240982294 1101 80-81 0.5778008585039949 1101 82-83 0.27726346116097744 1101 84-85 0.10522390651525271 1101 86-87 -0.08205649612718346 1101 88-89 -0.23345863330525063 1101 90-91 -3.771997180008615 1101 92-93 -8.767409025314922 1101 94-95 -7.182801994886544 1101 96-97 -3.840921974455707 1101 98-99 -2.659843719838495 1101 100-101 -2.1215006750299032 1102 1 1.1616744591010715 1102 2 1.0011406261298816 1102 3 1.0505533701650442 1102 4 1.1131945321717893 1102 5 1.075516221408293 1102 6 1.1272169003010575 1102 7 1.1393586732355416 1102 8 1.1391875520176384 1102 9 1.25238858462086 1102 10-11 1.1428675717034267 1102 12-13 1.065992828658068 1102 14-15 1.3418389161424997 1102 16-17 1.4607713219789389 1102 18-19 1.4939122669052267 1102 20-21 1.573598444989429 1102 22-23 1.7148186929020284 1102 24-25 1.4037821840378584 1102 26-27 1.375648336399884 1102 28-29 1.5602659138437147 1102 30-31 1.5398744491162546 1102 32-33 1.6087310264266321 1102 34-35 1.4049110813869632 1102 36-37 1.6815908699071827 1102 38-39 1.6481816211189724 1102 40-41 1.630240897556824 1102 42-43 1.6641222166947003 1102 44-45 1.4010607599795435 1102 46-47 1.2309183676413156 1102 48-49 1.2211948351377373 1102 50-51 1.185943964682167 1102 52-53 1.4246980714350705 1102 54-55 1.5838259949704785 1102 56-57 1.3679400460326896 1102 58-59 1.495318517144625 1102 60-61 1.4919406429753934 1102 62-63 1.3211944790117762 1102 64-65 1.3142274971651347 1102 66-67 1.3337713434892926 1102 68-69 0.9839326227627829 1102 70-71 0.8651163353913844 1102 72-73 0.9288625552693617 1102 74-75 0.9597962284697061 1102 76-77 0.7044592930767202 1102 78-79 0.8790991041063272 1102 80-81 0.5949704859966545 1102 82-83 0.4728437324434971 1102 84-85 -0.00843279794201024 1102 86-87 -0.37820953118471223 1102 88-89 -0.8166430817986878 1102 90-91 -4.867139232173139 1102 92-93 -9.711165562751237 1102 94-95 -8.101336892143351 1102 96-97 -4.995730575191276 1102 98-99 -3.889078291488799 1102 100-101 -3.263347648407777 1103 1 0.849705900978666 1103 2 0.8650792285300213 1103 3 0.8391923588040306 1103 4 0.9077752344447987 1103 5 0.7750049516662543 1103 6 0.8172821134431985 1103 7 0.7999702261547839 1103 8 0.6328444149052714 1103 9 0.8634190879590591 1103 10-11 0.8391088910216737 1103 12-13 0.8297850770657007 1103 14-15 0.850555378705117 1103 16-17 0.8742449354525519 1103 18-19 0.7848950194264432 1103 20-21 1.061364640447934 1103 22-23 0.7742592523425884 1103 24-25 0.7410861367264303 1103 26-27 0.574233096523109 1103 28-29 0.6819424585971845 1103 30-31 0.8426278749466078 1103 32-33 0.9030627976814856 1103 34-35 0.7946120249340609 1103 36-37 0.9422327920875659 1103 38-39 0.8267197611955694 1103 40-41 0.5320281608825468 1103 42-43 0.8011134306089858 1103 44-45 0.7888902785782932 1103 46-47 0.7735994564762549 1103 48-49 0.755937592957423 1103 50-51 0.6414888586885965 1103 52-53 0.46786959152967 1103 54-55 0.49598743020883873 1103 56-57 0.40438671324858433 1103 58-59 0.4695466452189052 1103 60-61 0.4070340042225986 1103 62-63 0.5285011940107971 1103 64-65 0.3677836891828683 1103 66-67 0.0443970310380557 1103 68-69 -0.12447368564352956 1103 70-71 0.1721931809297672 1103 72-73 0.23217862012388935 1103 74-75 -0.08027197313695922 1103 76-77 -0.23088503457529796 1103 78-79 -0.15581165388135432 1103 80-81 -0.2914423388777081 1103 82-83 -0.39967903238696323 1103 84-85 -0.4591253947884546 1103 86-87 -0.8225434832109748 1103 88-89 -1.2655017306573342 1103 90-91 -3.0324270051532203 1103 92-93 -9.305224979887576 1103 94-95 -5.995914525182524 1103 96-97 -3.5576806371413383 1103 98-99 -2.4936306114257327 1103 100-101 -2.00669799175812 1104 1 0.7057191346914884 1104 2 0.677562789618289 1104 3 0.6722536193100872 1104 4 0.6934268149277258 1104 5 0.6777386330944282 1104 6 0.6096526483519469 1104 7 0.7683390731938573 1104 8 0.7445048567040331 1104 9 0.7753070867191738 1104 10-11 0.6151826312424618 1104 12-13 0.601523469709214 1104 14-15 0.8242254035762997 1104 16-17 0.8766071893920966 1104 18-19 0.5957964855086928 1104 20-21 0.6600470386935413 1104 22-23 0.5760174310723727 1104 24-25 0.4592240612462959 1104 26-27 0.40841517263675087 1104 28-29 0.588147896773016 1104 30-31 0.5904262627595571 1104 32-33 0.6279744022946119 1104 34-35 0.5392206275261628 1104 36-37 0.47843629577469216 1104 38-39 0.47495476650410495 1104 40-41 0.46848939842362114 1104 42-43 0.5986648034454802 1104 44-45 0.5280475259252952 1104 46-47 0.45434179106474204 1104 48-49 0.5408720833638796 1104 50-51 0.5710942569302446 1104 52-53 0.6299286963817856 1104 54-55 0.684411438458131 1104 56-57 0.7440031754081211 1104 58-59 0.7755995868578722 1104 60-61 0.4103352205182347 1104 62-63 0.3371999166260444 1104 64-65 0.4768505302929036 1104 66-67 0.223366932769423 1104 68-69 0.3678476103055317 1104 70-71 0.4561513767418859 1104 72-73 0.48706065873560433 1104 74-75 0.19180141978089082 1104 76-77 0.1434917008788439 1104 78-79 0.21219371291071454 1104 80-81 -0.08679266516712758 1104 82-83 -0.18247215126355343 1104 84-85 -0.350080755047383 1104 86-87 -0.6138865331077703 1104 88-89 -0.48764184081006334 1104 90-91 -0.8318440000324827 1104 92-93 -4.209051619154643 1104 94-95 -1.9159587695412519 1104 96-97 -0.876342259430718 1104 98-99 -0.6378695117311217 1104 100-101 -0.5306992271158215 1105 1 0.8615765976613545 1105 2 0.906408833634508 1105 3 0.9748038186966426 1105 4 0.9930945599951357 1105 5 0.6683723363091403 1105 6 0.664059813501801 1105 7 0.8983048398494375 1105 8 0.9858818501496387 1105 9 1.134251597793778 1105 10-11 0.9197334151826908 1105 12-13 0.9503373968684485 1105 14-15 0.9991732472787405 1105 16-17 1.0319506482700902 1105 18-19 1.009210312554714 1105 20-21 1.0783010709380676 1105 22-23 0.9898453169701895 1105 24-25 1.040929714955361 1105 26-27 0.9573060324345803 1105 28-29 1.0375898831804662 1105 30-31 1.281800400626878 1105 32-33 1.282546855034795 1105 34-35 1.172081446640398 1105 36-37 1.396660092484396 1105 38-39 1.3351641307851878 1105 40-41 1.098617261140852 1105 42-43 1.3193405340535875 1105 44-45 1.4688648260264188 1105 46-47 1.2810191073586736 1105 48-49 1.144122235381431 1105 50-51 1.033451855960024 1105 52-53 1.149197970695358 1105 54-55 1.1576235944869318 1105 56-57 0.9703841834608475 1105 58-59 0.7936376030036456 1105 60-61 0.7900280274845031 1105 62-63 0.9302615332864974 1105 64-65 0.846954473342592 1105 66-67 0.7362279534538878 1105 68-69 0.8247115806343928 1105 70-71 0.7635778959935209 1105 72-73 0.8935009956266526 1105 74-75 0.9214268686945921 1105 76-77 0.5996400968837214 1105 78-79 0.4564859158381829 1105 80-81 0.552802279451452 1105 82-83 0.5258716805193693 1105 84-85 0.28862896030185325 1105 86-87 -0.02649071972149386 1105 88-89 -0.006978305201002399 1105 90-91 -1.167291301175048 1105 92-93 -6.929961675157571 1105 94-95 -2.9221108610003874 1105 96-97 -1.0967845045199631 1105 98-99 -0.36742021168375416 1105 100-101 -0.11376212038774369 1106 1 0.9706988681254813 1106 2 0.8180806430698979 1106 3 0.8033985230101948 1106 4 0.9723459913232446 1106 5 0.8744690203610972 1106 6 0.8302122032963624 1106 7 0.9082044420813062 1106 8 0.8526240654541581 1106 9 1.1064888387211198 1106 10-11 0.8813060101418628 1106 12-13 0.904554467219711 1106 14-15 0.9720922463958246 1106 16-17 1.0342293954370092 1106 18-19 0.8968767698697349 1106 20-21 0.9943114657024523 1106 22-23 0.9421691202524585 1106 24-25 0.8286840089396819 1106 26-27 0.7891156498671918 1106 28-29 1.007382260960064 1106 30-31 0.8266535358953462 1106 32-33 0.8459990636946699 1106 34-35 0.9248772013530839 1106 36-37 0.9824455707618824 1106 38-39 1.0263212992586475 1106 40-41 1.0031137704296995 1106 42-43 1.0068572594297436 1106 44-45 0.7235524824712698 1106 46-47 1.0363930731160238 1106 48-49 0.9119394258823306 1106 50-51 0.6657930445312417 1106 52-53 0.8605339072709093 1106 54-55 1.003815214959701 1106 56-57 1.009781887874528 1106 58-59 0.9819667037928141 1106 60-61 0.8787890298237819 1106 62-63 1.0519945098118058 1106 64-65 0.9880474709851086 1106 66-67 0.9217285314464796 1106 68-69 1.1624727013028604 1106 70-71 1.1889863592614098 1106 72-73 1.2438236702304764 1106 74-75 1.0744647431382184 1106 76-77 0.93314182175925 1106 78-79 0.7823331073403281 1106 80-81 0.8350952261213926 1106 82-83 1.1900147496145124 1106 84-85 0.9767600872508737 1106 86-87 0.7058668528916705 1106 88-89 0.5489763838207793 1106 90-91 0.16117369613978738 1106 92-93 -3.506992158577832 1106 94-95 -1.5320291228355813 1106 96-97 -0.37552575498645524 1106 98-99 -0.44457994699045145 1106 100-101 -0.1876873727475008 1107 1 0.5886220086225684 1107 2 0.6766476848232372 1107 3 0.6545072981459548 1107 4 0.5489578512770876 1107 5 0.4084026106517129 1107 6 0.4421120757047987 1107 7 0.37199992891778777 1107 8 0.3968433396163462 1107 9 0.2818058586670915 1107 10-11 0.23839532571587085 1107 12-13 0.47592206251049873 1107 14-15 0.6198749178941227 1107 16-17 0.6078955609039056 1107 18-19 0.26744111490216227 1107 20-21 0.33567567530744213 1107 22-23 0.2913625434469225 1107 24-25 0.4298913237951538 1107 26-27 0.27691919729711145 1107 28-29 0.2538458456177253 1107 30-31 0.3398853577934915 1107 32-33 0.04083167923304387 1107 34-35 0.0712584354873016 1107 36-37 0.07223262738858693 1107 38-39 0.01772786689770811 1107 40-41 0.10460332433232367 1107 42-43 -0.07363941624078763 1107 44-45 -0.16189306047298402 1107 46-47 0.009349575185133574 1107 48-49 0.2941134205628124 1107 50-51 0.35871341290568637 1107 52-53 0.3757946272539954 1107 54-55 0.3560121406387218 1107 56-57 0.2693429019243858 1107 58-59 0.26573137941010927 1107 60-61 0.09699026966487523 1107 62-63 0.17448492565977247 1107 64-65 0.36225357202047803 1107 66-67 0.185455565334383 1107 68-69 0.023254040703815093 1107 70-71 -0.08295680261429084 1107 72-73 0.05526093081148531 1107 74-75 0.11588646504774758 1107 76-77 -0.26507012217142645 1107 78-79 -0.22497154672094055 1107 80-81 -0.31295342130452397 1107 82-83 -0.19032639149466135 1107 84-85 -0.2058349743789556 1107 86-87 -0.18900834245057396 1107 88-89 -0.34704447436925534 1107 90-91 -0.5815682953826915 1107 92-93 -3.6362651823500762 1107 94-95 -1.2281811209076636 1107 96-97 -0.4337552630862298 1107 98-99 -0.3585946163242717 1107 100-101 -0.3332354916220339 1108 1 0.4357276853748182 1108 2 0.35879782390959036 1108 3 0.08345855712842365 1108 4 -0.05955585987416612 1108 5 -0.6082358307970495 1108 6 -0.07356305269941288 1108 7 0.12608291662134263 1108 8 0.041415660677600385 1108 9 0.3939353381124846 1108 10-11 0.2290174275316943 1108 12-13 0.15879895791136533 1108 14-15 0.255472917678496 1108 16-17 0.31120613657914475 1108 18-19 0.0843472654566142 1108 20-21 0.29853348722922135 1108 22-23 0.10072987207507822 1108 24-25 0.17228422359655582 1108 26-27 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0.5204215183249303 2311 72-73 0.4178740606622888 2311 74-75 0.517049325087104 2311 76-77 0.6355561601149091 2311 78-79 0.5097253293535751 2311 80-81 0.377362168632839 2311 82-83 0.6956437941188653 2311 84-85 0.7780661704640472 2311 86-87 0.8251620555609556 2311 88-89 0.9310701571125932 2311 90-91 1.582830297258493 2311 92-93 2.5874393991789084 2311 94-95 1.8038592575515757 2311 96-97 1.1417842011988064 2311 98-99 0.8743606976225635 2311 100-101 0.7731795878749619 2312 1 0.07167450285536248 2312 2 -0.07596523154906976 2312 3 -0.07822630800754382 2312 4 -0.1961602816026513 2312 5 0.02340835832423238 2312 6 0.06772827131267434 2312 7 0.10920263084054227 2312 8 -0.7096606162088648 2312 9 -0.27083896541581964 2312 10-11 0.02714271620198616 2312 12-13 -0.0618728223255971 2312 14-15 -0.35222902260732525 2312 16-17 -0.06455351718756219 2312 18-19 -0.003956899006674064 2312 20-21 0.28221337366264265 2312 22-23 -0.016118655325271902 2312 24-25 0.24712504122238244 2312 26-27 0.12410898743460308 2312 28-29 0.022952981678912465 2312 30-31 0.3053384561052752 2312 32-33 0.13841495999594144 2312 34-35 -0.02067861710219887 2312 36-37 0.3347558367195518 2312 38-39 0.4034740675236108 2312 40-41 0.32009252674793487 2312 42-43 0.38210829407854163 2312 44-45 0.046883172970481723 2312 46-47 0.3113111936873736 2312 48-49 0.1573243595139857 2312 50-51 0.33544611374720645 2312 52-53 0.07553399721028953 2312 54-55 0.20049506812328488 2312 56-57 0.17882115540821175 2312 58-59 0.290130570577368 2312 60-61 0.4374538589597101 2312 62-63 0.28096619827839575 2312 64-65 0.44801033293923 2312 66-67 0.40882264539829194 2312 68-69 0.1349774023651129 2312 70-71 0.21148407272561087 2312 72-73 0.43754617818319375 2312 74-75 0.6074938046276976 2312 76-77 0.5240864481579806 2312 78-79 0.427122503902428 2312 80-81 0.4110519389892282 2312 82-83 0.8418753186678956 2312 84-85 0.9313563355333763 2312 86-87 0.667089142914346 2312 88-89 0.8280004446806828 2312 90-91 1.3096647208814183 2312 92-93 2.3891948267786525 2312 94-95 1.9619637567328176 2312 96-97 1.6524644872825611 2312 98-99 1.3698730347767594 2312 100-101 1.6206942857070104 2313 1 -0.1955850030042079 2313 2 -0.6634384904316164 2313 3 -0.11096321249691243 2313 4 -0.1023681670913561 2313 5 -0.9755454129132488 2313 6 -0.45350831830962335 2313 7 -1.0806304789122052 2313 8 -0.8937343566751892 2313 9 -1.0900622362440515 2313 10-11 -1.9664668728733048 2313 12-13 -0.5876841963845933 2313 14-15 -0.17656671415740988 2313 16-17 -1.5578975671745283 2313 18-19 -1.1312216776119755 2313 20-21 -1.3818711214933543 2313 22-23 -1.1782307442002704 2313 24-25 -0.2286939043853593 2313 26-27 -0.8028795306742396 2313 28-29 -2.3224582199817405 2313 30-31 -0.5614233244282119 2313 32-33 -0.36287802756127974 2313 34-35 -0.09260714168191697 2313 36-37 0.002148872844045968 2313 38-39 -0.312137729068219 2313 40-41 -0.1584297113781048 2313 42-43 -0.10117391644284623 2313 44-45 -0.5195038575013697 2313 46-47 -0.7615172397722603 2313 48-49 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70-71 -0.9645877553529374 2314 72-73 -0.8529809172512444 2314 74-75 -0.6217448808437318 2314 76-77 -0.7066871821521872 2314 78-79 -0.6045830078387873 2314 80-81 -0.5972127178155979 2314 82-83 -0.544932925470551 2314 84-85 -0.6968171587503171 2314 86-87 -0.6581617601555898 2314 88-89 -0.495764843403272 2314 90-91 0.13544264312708876 2314 92-93 1.0773033772388025 2314 94-95 0.7290218768317089 2314 96-97 0.6280475208635643 2314 98-99 0.35601921033098094 2314 100-101 0.35388793850396283 2315 1 -6.88547903970024 2315 2 -2.8855263655051395 2315 3 -1.3828536718991558 2315 4 -0.9320785815715666 2315 5 -0.9915457249453397 2315 6 -1.5641287493328306 2315 7 -2.879347391412484 2315 8 -2.846515908452176 2315 9 -3.202636238529884 2315 10-11 -2.217863252578802 2315 12-13 -2.74350245117661 2315 14-15 -4.499830150858589 2315 16-17 -4.224952227443573 2315 18-19 -2.8738551561991343 2315 20-21 -4.021120376143557 2315 22-23 -5.197380028199792 2315 24-25 -3.3121024167459083 2315 26-27 -4.007432334039368 2315 28-29 -2.614699262843054 2315 30-31 -2.222319323603827 2315 32-33 -1.372980788684103 2315 34-35 -2.15596819434721 2315 36-37 -2.6756480217143945 2315 38-39 -1.7168797018862776 2315 40-41 -3.3416494876636165 2315 42-43 -7.398511001865721 2315 44-45 -4.076006223383555 2315 46-47 -2.386533762304662 2315 48-49 -5.0824041893663505 2315 50-51 -6.108193163099827 2315 52-53 -6.511858652529423 2315 54-55 -4.162329998701498 2315 56-57 -3.494812672253726 2315 58-59 -3.8465961963883863 2315 60-61 -2.663001904097012 2315 62-63 -2.7537746859229593 2315 64-65 -1.7249598456129576 2315 66-67 -2.378054254858686 2315 68-69 -1.7713096337583742 2315 70-71 -1.3974610476975542 2315 72-73 -1.889028429665398 2315 74-75 -2.4674458685215335 2315 76-77 -2.6301948888966926 2315 78-79 -1.44147449457844 2315 80-81 -1.0513640925563195 2315 82-83 -1.4782754142486922 2315 84-85 -1.2118324100479718 2315 86-87 -1.7113193749192526 2315 88-89 -1.09838503033847 2315 90-91 -0.49796602551837665 2315 92-93 0.33528659526019666 2315 94-95 0.24119865865585055 2315 96-97 -0.029773964374850692 2315 98-99 0.010292156184647183 2315 100-101 -0.06356390007508494 2316 1 -4.555627342700731 2316 2 -3.4319734879842336 2316 3 -4.876632816021143 2316 4 -4.484740903263649 2316 5 -4.257681976789904 2316 6 -4.340374691290531 2316 7 -3.1519821676053006 2316 8 -1.8627448804147875 2316 9 -1.95426757153529 2316 10-11 -2.9590828787470187 2316 12-13 -3.9735829971677603 2316 14-15 -3.040360032806447 2316 16-17 -2.941935134477518 2316 18-19 -3.210835480842519 2316 20-21 -5.6132476511066685 2316 22-23 -6.5591813070979725 2316 24-25 -4.267493341487665 2316 26-27 -3.99179460654306 2316 28-29 -4.847854206276406 2316 30-31 -6.375016692024882 2316 32-33 -4.621736691291083 2316 34-35 -6.0033051348292545 2316 36-37 -9.233492463426145 2316 38-39 -6.298094655157307 2316 40-41 -5.526099192533266 2316 42-43 -4.82379444997197 2316 44-45 -3.261322873654091 2316 46-47 -5.7126582089352596 2316 48-49 -3.3780356453427416 2316 50-51 -4.529221200483001 2316 52-53 -5.275725351988349 2316 54-55 -6.916606437461951 2316 56-57 -4.905560704718063 2316 58-59 -5.652791843215738 2316 60-61 -6.254793591258842 2316 62-63 -6.4958715870542925 2316 64-65 -5.285533013345372 2316 66-67 -6.68309126912332 2316 68-69 -5.515924734594577 2316 70-71 -6.219057088032038 2316 72-73 -7.707062369655564 2316 74-75 -7.6412232910498155 2316 76-77 -6.84870361983619 2316 78-79 -5.9167544994972765 2316 80-81 -6.053763748237582 2316 82-83 -5.0576269882772245 2316 84-85 -6.569615981125196 2316 86-87 -5.340525597250782 2316 88-89 -5.122896835552439 2316 90-91 -5.6178426856265915 2316 92-93 -4.287673070258743 2316 94-95 -4.362557612864073 2316 96-97 -5.085236581197282 2316 98-99 -5.750360573771079 2316 100-101 -4.941444495254624 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10006.0 3 3913.0 4 4044.0 5 4590.0 6 5497.0 7 6616.0 8 8131.0 9 10263.0 10 12519.0 11 15127.0 12 18383.0 13 21420.0 14 23274.0 15 26370.0 16 30100.0 17 33724.0 18 29672.0 19 25992.0 20 27297.0 21 29333.0 22 32139.0 23 35758.0 24 39652.0 25 44624.0 26 50156.0 27 56535.0 28 64067.0 29 73272.0 30 82883.0 31 93714.0 32 107554.0 33 123916.0 34 145218.0 35 169291.0 36 185558.0 37 150714.0 38 42062.0 39 570.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.05076465878713 18.470356750301928 13.70626789518679 20.772610695724154 2 15.169337134742285 22.32835574586727 39.16446449858967 23.33784262080077 3 21.175450710127652 26.837038677584026 30.22265720843077 21.764853403857554 4 12.106931980850563 16.246350039057774 39.03719743798391 32.60952054210775 5 12.436652379525528 39.51078483739149 33.50421448950612 14.548348293576865 6 28.951419003630956 37.14516768069298 17.92921535530722 15.97419796036885 7 26.704445837641842 35.025145403669896 21.112640009553356 17.157768749134906 8 25.79544887090226 31.705110195857912 20.4325331260625 22.066907807177333 9 27.009739322242172 17.383148758051874 21.474597650491635 34.13251426921432 10-11 24.911510981540115 25.970665232242624 27.200066872240907 21.91775691397635 12-13 28.320824383022202 24.305241003665163 26.932378392241418 20.441556221071213 14-15 24.13186269174744 25.033512291730503 28.321828735701125 22.512796280820925 16-17 20.960737563321587 28.585273973996934 28.31208025913507 22.141908203546414 18-19 20.63063970203673 28.154983022032138 30.71458368220653 20.499793593724597 20-21 22.909312402231542 25.533056086894803 29.840744803257902 21.716886707615753 22-23 21.742877789474772 25.966227966115035 30.03118011552333 22.25971412888686 24-25 22.71196602222501 25.83740216211343 30.34936264519216 21.1012691704694 26-27 22.408570114303313 24.9641427468964 31.794508205757904 20.832778933042384 28-29 20.940230354481745 27.21298557134602 31.007893181907047 20.83889089226518 30-31 21.338371769694653 26.336981456700443 30.45719573869067 21.867451034914236 32-33 21.44892496330196 26.4049932143247 30.45695644405633 21.689125378317012 34-35 22.661641763892924 25.7230130953383 30.253679754764473 21.361665386004304 36-37 22.55589201306826 25.203105449742807 31.299364399416095 20.941638137772834 38-39 22.151475486215247 26.079344767750783 31.03982497310004 20.72935477293393 40-41 21.608796579108123 26.57324373854612 30.729627367135002 21.08833231521075 42-43 20.951552231795603 27.085491190512712 29.425275809781866 22.53768076790982 44-45 21.74636583259605 26.340075051967094 29.306129008701042 22.60743010673581 46-47 22.73479399862282 26.913281098963637 28.68460838342084 21.667316518992706 48-49 22.426227068465117 26.716448351275908 28.850084908224975 22.007239672034004 50-51 21.448068656938567 27.344410759614345 29.81168121825977 21.395839365187317 52-53 22.04612508739516 28.122010433637534 28.139041285283334 21.69282319368397 54-55 20.955966034867988 26.96589417050688 28.957333896847558 23.120805897777576 56-57 22.304330931981674 27.273502377411457 27.99667751623974 22.42548917436713 58-59 23.22268074434259 26.616842547111947 27.887026352064805 22.273450356480662 60-61 21.959569802323337 26.91388037207489 29.595380367866635 21.53116945773514 62-63 22.13045434195769 26.804190885034103 29.089271737209323 21.976083035798887 64-65 21.59558983607928 28.09449017252041 28.567174486285285 21.742745505115025 66-67 21.72794029811846 27.21126425597442 27.973637171197662 23.087158274709452 68-69 21.260854480328774 27.598790498824215 28.478252489071544 22.662102531775467 70-71 22.413083914737786 27.40542884285559 27.662190732516667 22.519296509889962 72-73 22.04077763981363 26.57727241618678 28.645458209355706 22.736491734643884 74-75 21.236208367059795 28.80456217167629 27.734505293922716 22.224724167341193 76-77 22.10440221914611 26.946153327958587 27.81326533051413 23.13617912238117 78-79 21.778722916483883 27.255930891757203 28.38584186081381 22.579504330945106 80-81 22.29518839760846 27.64942371058129 27.791030910192294 22.264356981617954 82-83 21.79722533403888 27.258545060614324 28.29937076712951 22.644858838217285 84-85 21.55331521361282 27.5431972833455 27.86262796781034 23.04085953523134 86-87 21.86029736861259 27.744391613192676 27.666366842976032 22.7289441752187 88-89 21.876276685442093 27.311724209867528 27.187315288063697 23.624683816626682 90-91 21.581788132674067 26.762260084165778 28.104812739433267 23.55113904372689 92-93 21.669206465172955 27.776145158344395 28.248493158900516 22.30615521758213 94-95 21.776081199609823 27.580589651156885 27.508551780750622 23.13477736848267 96-97 20.843393100143253 27.41278871542064 28.075558345923042 23.668259838513066 98-99 21.775510070994752 27.84799601768565 26.62716234017486 23.74933157114474 100-101 21.658721447960065 27.154387062359664 27.381751748849386 23.80513974083089 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4628.0 1 3121.0 2 1702.0 3 1716.0 4 1467.5 5 1469.0 6 2176.5 7 3746.5 8 5761.0 9 6447.5 10 5293.5 11 4216.0 12 3950.5 13 3905.5 14 4034.0 15 4247.5 16 4373.0 17 4490.0 18 4743.0 19 5100.5 20 5474.0 21 5919.5 22 6610.5 23 7482.5 24 8417.0 25 9664.5 26 11354.0 27 13474.5 28 15597.0 29 17525.0 30 19700.0 31 22306.5 32 25192.5 33 27867.0 34 30842.0 35 33682.5 36 36630.0 37 40131.5 38 42120.5 39 42611.5 40 42962.5 41 44712.0 42 47257.5 43 49761.5 44 52701.5 45 55952.5 46 62736.0 47 82159.5 48 119911.5 49 119950.5 50 83597.5 51 65846.0 52 59216.0 53 56819.0 54 54654.5 55 51114.5 56 47029.5 57 42453.0 58 38701.5 59 35317.5 60 31244.5 61 26483.0 62 21316.0 63 16218.0 64 11554.0 65 8130.5 66 5757.0 67 4150.5 68 2941.5 69 1890.0 70 1295.5 71 923.5 72 654.0 73 485.0 74 369.0 75 274.5 76 197.5 77 127.0 78 65.0 79 38.0 80 25.0 81 16.5 82 13.0 83 11.0 84 7.5 85 5.5 86 6.5 87 4.0 88 2.0 89 2.5 90 3.5 91 3.0 92 1.0 93 1.5 94 1.5 95 0.0 96 1.5 97 2.5 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08834276776969491 2 0.09924325661052281 3 0.09490475358929779 4 0.09821286214298187 5 0.09425397813611402 6 0.07939460528841827 7 0.09051201928030742 8 0.0866615978489702 9 0.08275694512986766 10-11 0.08866815549628679 12-13 0.08712256379497536 14-15 0.09325069931245573 16-17 0.0906475974997207 18-19 0.09292531158586385 20-21 0.10360887527563051 22-23 0.11252992211302451 24-25 0.13639168872976226 26-27 0.14235713038394668 28-29 0.14197751136958947 30-31 0.143279062275957 32-33 0.139645565995681 34-35 0.1333547366149047 36-37 0.13807285865048696 38-39 0.1306160565827564 40-41 0.12630466920541403 42-43 0.1264402474248273 44-45 0.11732939108025471 46-47 0.13612053229093568 48-49 0.15447782319949413 50-51 0.16448349579219437 52-53 0.17229280123039944 54-55 0.16947277426660318 56-57 0.13885921232308396 58-59 0.1306702878705217 60-61 0.12665717257588854 62-63 0.12421676462644946 64-65 0.1099268203002895 66-67 0.12310502322726055 68-69 0.11947152694698458 70-71 0.12822987992108262 72-73 0.1570538093683465 74-75 0.17158779448945038 76-77 0.17603476008620605 78-79 0.17297069232746587 80-81 0.17890901833776765 82-83 0.17896324962553295 84-85 0.1554268707353871 86-87 0.155752258461979 88-89 0.1762516852372673 90-91 0.2627777048668242 92-93 0.5324970145676085 94-95 0.17654995731997652 96-97 0.18956546638365165 98-99 0.2070550566879651 100-101 0.21467455261899157 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1843954.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.18124060587613 #Duplication Level Percentage of deduplicated Percentage of total 1 74.75005577121178 34.520503108729905 2 12.362325626817434 11.418150684404887 3 4.863032587629268 6.737426340105708 4 2.4023933860837956 4.43782027970805 5 1.4356520767387573 3.3150096991099143 6 0.91530585351129 2.536197590938302 7 0.6098642648962777 1.9715001847870541 8 0.4589279554641637 1.6955089865642679 9 0.3429475026405558 1.4253967021165062 >10 1.7091340076666184 13.842479453213501 >50 0.09260243460204308 2.955285083492432 >100 0.04919766581349889 4.025824149693735 >500 0.0030143897621799447 0.9440547284689049 >1k 0.004220145667051923 4.502273508927292 >5k 8.440291334103845E-4 2.6333747917159047 >10k+ 4.823023619487912E-4 3.039194708023642 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 19313 1.0473688606114904 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14752 0.8000199571138976 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 10429 0.5655781001044494 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10086 0.5469767684009471 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 7977 0.43260298250390194 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 7937 0.43043373099328947 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7440 0.4034807809739288 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 7081 0.3840117486661815 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 6107 0.3311904743827666 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 5740 0.3112875917728967 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 5010 0.2716987517042182 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 4720 0.25597167825227746 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 4501 0.2440950262316739 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 3828 0.20759736956561825 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3766 0.20423502972416885 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3538 0.19187029611367745 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3228 0.17505859690643044 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2979 0.16155500625286748 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2976 0.16139231238957155 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2944 0.15965691118108152 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2826 0.15325761922477457 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2641 0.1432248309881917 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 2606 0.1413267359164057 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2480 0.13449359365797628 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 2455 0.13313781146384346 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 2411 0.1307516348021697 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 2269 0.12305079193949524 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2218 0.12028499626346428 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2147 0.11643457483212705 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2073 0.1124214595374939 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 2052 0.1112826024944223 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2042 0.11074028961676918 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1954 0.10596793629342163 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 5.423128776531302E-5 0.0 10-11 0.0 0.0 0.0 1.0846257553062604E-4 0.0 12-13 0.0 0.0 0.0 1.8980950717859555E-4 0.0 14-15 0.0 0.0 0.0 3.253877265918781E-4 0.0 16-17 0.0 0.0 0.0 4.3385030212250415E-4 0.0 18-19 0.0 0.0 0.0 6.778910970664128E-4 0.0 20-21 0.0 0.0 0.0 0.0011930883308368864 0.0 22-23 0.0 0.0 0.0 0.0024675235933217425 0.0 24-25 0.0 0.0 0.0 0.004067346582398476 0.0 26-27 0.0 0.0 0.0 0.005640053927592554 0.0 28-29 0.0 0.0 0.0 0.010927604484710573 0.0 30-31 0.0 0.0 0.0 0.024404079494390857 0.0 32-33 0.0 0.0 0.0 0.05073336970445033 0.0 34-35 0.0 0.0 0.0 0.08695986993167942 0.0 36-37 5.423128776531302E-5 0.0 0.0 0.13937440955685446 0.0 38-39 5.423128776531302E-5 0.0 0.0 0.2176030421583185 0.0 40-41 5.423128776531302E-5 0.0 0.0 0.301553075619023 0.0 42-43 5.423128776531302E-5 0.0 0.0 0.38791640138528405 0.0 44-45 5.423128776531302E-5 0.0 0.0 0.4886781340532356 0.0 46-47 5.423128776531302E-5 0.0 0.0 0.6102646812230674 0.0 48-49 5.423128776531302E-5 0.0 0.0 0.7405011187914666 0.0 50-51 5.423128776531302E-5 0.0 0.0 0.8784112835786576 0.0 52-53 5.423128776531302E-5 0.0 0.0 1.009895040765659 0.0 54-55 5.423128776531302E-5 0.0 0.0 1.1525775588761975 0.0 56-57 5.423128776531302E-5 0.0 0.0 1.3315408085017304 0.0 58-59 5.423128776531302E-5 0.0 0.0 1.5251193901800153 0.0 60-61 5.423128776531302E-5 0.0 0.0 1.7518332886829064 0.0 62-63 5.423128776531302E-5 0.0 0.0 1.9724461673121998 0.0 64-65 5.423128776531302E-5 0.0 0.0 2.18516839357164 0.0 66-67 5.423128776531302E-5 0.0 0.0 2.4245181821238493 0.0 68-69 1.0846257553062604E-4 0.0 0.0 2.698277722763149 0.0 70-71 1.0846257553062604E-4 0.0 0.0 3.007721450752025 0.0 72-73 1.0846257553062604E-4 2.711564388265651E-5 0.0 3.3213409878988305 0.0 74-75 1.3557821941328254E-4 5.423128776531302E-5 0.0 3.6476777620266017 0.0 76-77 1.6269386329593904E-4 5.423128776531302E-5 0.0 3.971818168999877 0.0 78-79 1.6269386329593904E-4 5.423128776531302E-5 0.0 4.378281670800899 0.0 80-81 1.6269386329593904E-4 5.423128776531302E-5 0.0 4.813948721063541 0.0 82-83 1.6269386329593904E-4 5.423128776531302E-5 0.0 5.219381828397021 0.0 84-85 1.6269386329593904E-4 5.423128776531302E-5 0.0 5.6530152053684635 0.0 86-87 1.6269386329593904E-4 5.423128776531302E-5 0.0 6.108178403582736 0.0 88-89 1.6269386329593904E-4 5.423128776531302E-5 0.0 6.583488525201822 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 11685 0.0 27.006575 1 TACATGG 11710 0.0 26.544394 2 TTTGCGT 550 0.0 25.022095 3 ACATGGG 12105 0.0 24.42371 3 CATGGGA 8375 0.0 24.083305 4 GAGTACT 9875 0.0 23.424316 12-13 AGTACTT 10055 0.0 22.34433 12-13 GTACTTT 10960 0.0 21.302814 14-15 ATGGGAT 3050 0.0 21.006123 5 ATGGGAG 2765 0.0 20.081802 5 ACTTTTT 12450 0.0 19.592684 16-17 CTTTGCG 710 0.0 19.383312 2 TACTTTT 11820 0.0 18.990051 14-15 TATAACG 155 9.6880394E-4 18.36995 2 TACCTGG 1090 0.0 18.285683 2 CATGGGG 3710 0.0 18.036703 4 AGTACAT 8565 0.0 17.89631 2 GTATTAG 320 8.378265E-9 17.795404 1 GTCGGGA 440 5.456968E-12 17.256618 2 TTGCGTT 825 0.0 16.682302 4 >>END_MODULE